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The map label for this gene is murF [H]

Identifier: 55981048

GI number: 55981048

Start: 1025984

End: 1027300

Strand: Direct

Name: murF [H]

Synonym: TTHA1079

Alternate gene names: 55981048

Gene position: 1025984-1027300 (Clockwise)

Preceding gene: 55981047

Following gene: 55981049

Centisome position: 55.47

GC content: 74.03

Gene sequence:

>1317_bases
ATGCTGAAGGGCGGTGAGGATGTTGTGCAAGGGACGCATGTTAGGGAACTAACGCCGGAGTGGGTGGCCCGGGCCGTGGG
GGGACGGCTCCATCCGGGCGGAGGGCCGGTGCGGGACCTGGTCTGGCACAGCGGCGCCGCCACGCCGGGAAGCCTCTTCG
CCGCCCTCCCCGGGCGCACCACCCACGGGCGGCATTTCATCCCGGAGGCCCTGGCCCGGGGGGCAAGCCTGGTCCTGGCC
GACCAGGCGGGCCCCGCCACCCTGGAGGTGGAGGACCCCTGGCAGGCCCTCCTGCGCCTGGGCGAGGCCCTCCGCCGCCT
CTTCCCGGGCCCGGTCCTGGCCGTGGGGGGAAGCTCGGGCAAGACCACCACCAAGGAGGCCCTGGCCCAGGGCCTGGGCC
TTCCCGCCCCCCCGGGGAACCTCAACACCGCCCCGCCCCTTGTCCGCTTCTTCTGGCACCTGGACCCCCAAAGCCCCGGG
GCCGTGGTGGAGCTCGGCGTGGACCGCCAGGGGGAGATGGCGGAGCTCTTCGCCCTGGCGAAGCCCCACCTCGCCGTCCT
CACCGCCCTCGGGGAGGAGCACCTGGAGGCCTTCGGCACCCTGGAGAAGGCGGTGCAGGAGGAGGCCCACCTCCTCCGGG
CCCCCACCGCTTTGGTGGGCGAGCAGGCCGCCCCCCTCGTCCTGGGGCTCAGGGAAGACCTCCTCGTCTACGGGTTCGCC
GAGGGCACCTTCCGGGGACGGGACCTGGAGCTCGGCCTCCGGGAAAGCCGCTTCCGCTACGGAGGCCTTCACGTGCGCGT
TCCCTACCCCGGGCTGGGCCCCGCCTACGGGGCGCTCGCGGCCCTGGCGGCGGCCGAGGTCCTGGGCCTTTCCCTGGAAG
AGGTGGCGGAAAGGCTCGCCCGCCTCACCCTCCCCCCGGGGCGCATGGAGACGCGGGAGGTGGGCGGCGTGGTCTTCGTC
AACGACGCCTACAACGCCAACCCCCTTTCGGTGAAGGCCGGCCTCCGCTGGCTCGCCGCCCAGCCGGGGCGGAAGTGGGC
CGTGCTCGGGGAGATGCGGGAGCTCGGCAGGGAAAGCCTCCGCCTCCACCTCGAGGTGGCGGAGGAGGCGGCGAGGCTGG
GCCTCTCCCCCCTCTACCTGGGGCCCCACGCCCGGCACCAGGCCGCCTTCTCCGGCGAGGCGGTGGAGAGCCTGGAGGAA
GCCCTGGCCTGGCTCAAGGCCCGGGTGGCCCCGGGAGACCTCGTCTACCTCAAGGCCTCCAGGGCCGTGGGCCTGGAGCG
GATCGTGGAGCTATGGGACGCGCCCTTCTCCTCCTAG

Upstream 100 bases:

>100_bases
GTCCACCACCCCAAAACCCGGATCCACGGGAGCCAGGTGGCCGCCCCCATCTTCCGGGAGATCGCCGCCGGGCTCTTGGC
CTGGGCGGGCCTGCCCCCCT

Downstream 100 bases:

>100_bases
TCCTCCTCGCGGCCTGCGCCCCCCTGCCCGAGGGGCCAGGCCTGCCCTACCCCGGAGGGTTCGTCCGGGGAAGCGTGGTG
GAGGGCCGCTTCCAGGCCAT

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6- diaminopimelate--D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MLKGGEDVVQGTHVRELTPEWVARAVGGRLHPGGGPVRDLVWHSGAATPGSLFAALPGRTTHGRHFIPEALARGASLVLA
DQAGPATLEVEDPWQALLRLGEALRRLFPGPVLAVGGSSGKTTTKEALAQGLGLPAPPGNLNTAPPLVRFFWHLDPQSPG
AVVELGVDRQGEMAELFALAKPHLAVLTALGEEHLEAFGTLEKAVQEEAHLLRAPTALVGEQAAPLVLGLREDLLVYGFA
EGTFRGRDLELGLRESRFRYGGLHVRVPYPGLGPAYGALAALAAAEVLGLSLEEVAERLARLTLPPGRMETREVGGVVFV
NDAYNANPLSVKAGLRWLAAQPGRKWAVLGEMRELGRESLRLHLEVAEEAARLGLSPLYLGPHARHQAAFSGEAVESLEE
ALAWLKARVAPGDLVYLKASRAVGLERIVELWDAPFSS

Sequences:

>Translated_438_residues
MLKGGEDVVQGTHVRELTPEWVARAVGGRLHPGGGPVRDLVWHSGAATPGSLFAALPGRTTHGRHFIPEALARGASLVLA
DQAGPATLEVEDPWQALLRLGEALRRLFPGPVLAVGGSSGKTTTKEALAQGLGLPAPPGNLNTAPPLVRFFWHLDPQSPG
AVVELGVDRQGEMAELFALAKPHLAVLTALGEEHLEAFGTLEKAVQEEAHLLRAPTALVGEQAAPLVLGLREDLLVYGFA
EGTFRGRDLELGLRESRFRYGGLHVRVPYPGLGPAYGALAALAAAEVLGLSLEEVAERLARLTLPPGRMETREVGGVVFV
NDAYNANPLSVKAGLRWLAAQPGRKWAVLGEMRELGRESLRLHLEVAEEAARLGLSPLYLGPHARHQAAFSGEAVESLEE
ALAWLKARVAPGDLVYLKASRAVGLERIVELWDAPFSS
>Mature_438_residues
MLKGGEDVVQGTHVRELTPEWVARAVGGRLHPGGGPVRDLVWHSGAATPGSLFAALPGRTTHGRHFIPEALARGASLVLA
DQAGPATLEVEDPWQALLRLGEALRRLFPGPVLAVGGSSGKTTTKEALAQGLGLPAPPGNLNTAPPLVRFFWHLDPQSPG
AVVELGVDRQGEMAELFALAKPHLAVLTALGEEHLEAFGTLEKAVQEEAHLLRAPTALVGEQAAPLVLGLREDLLVYGFA
EGTFRGRDLELGLRESRFRYGGLHVRVPYPGLGPAYGALAALAAAEVLGLSLEEVAERLARLTLPPGRMETREVGGVVFV
NDAYNANPLSVKAGLRWLAAQPGRKWAVLGEMRELGRESLRLHLEVAEEAARLGLSPLYLGPHARHQAAFSGEAVESLEE
ALAWLKARVAPGDLVYLKASRAVGLERIVELWDAPFSS

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=414, Percent_Identity=30.1932367149758, Blast_Score=118, Evalue=7e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 46672; Mature: 46672

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKGGEDVVQGTHVRELTPEWVARAVGGRLHPGGGPVRDLVWHSGAATPGSLFAALPGRT
CCCCCCHHHCCCCHHHCCHHHHHHHHCCEECCCCCCHHHHHHCCCCCCCCHHHEECCCCC
THGRHFIPEALARGASLVLADQAGPATLEVEDPWQALLRLGEALRRLFPGPVLAVGGSSG
CCCCCCCHHHHHCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHCCCCEEEECCCCC
KTTTKEALAQGLGLPAPPGNLNTAPPLVRFFWHLDPQSPGAVVELGVDRQGEMAELFALA
CCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHH
KPHLAVLTALGEEHLEAFGTLEKAVQEEAHLLRAPTALVGEQAAPLVLGLREDLLVYGFA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCEEEECCCCEEEEEEC
EGTFRGRDLELGLRESRFRYGGLHVRVPYPGLGPAYGALAALAAAEVLGLSLEEVAERLA
CCCCCCCCEECCCHHCCCEECCEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHH
RLTLPPGRMETREVGGVVFVNDAYNANPLSVKAGLRWLAAQPGRKWAVLGEMRELGRESL
HCCCCCCCCCHHHCCCEEEEECCCCCCCEEHHHHHHHHHCCCCCCCHHHHHHHHHCHHHH
RLHLEVAEEAARLGLSPLYLGPHARHQAAFSGEAVESLEEALAWLKARVAPGDLVYLKAS
EEHHHHHHHHHHCCCCCEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEECC
RAVGLERIVELWDAPFSS
HHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLKGGEDVVQGTHVRELTPEWVARAVGGRLHPGGGPVRDLVWHSGAATPGSLFAALPGRT
CCCCCCHHHCCCCHHHCCHHHHHHHHCCEECCCCCCHHHHHHCCCCCCCCHHHEECCCCC
THGRHFIPEALARGASLVLADQAGPATLEVEDPWQALLRLGEALRRLFPGPVLAVGGSSG
CCCCCCCHHHHHCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHCCCCEEEECCCCC
KTTTKEALAQGLGLPAPPGNLNTAPPLVRFFWHLDPQSPGAVVELGVDRQGEMAELFALA
CCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHH
KPHLAVLTALGEEHLEAFGTLEKAVQEEAHLLRAPTALVGEQAAPLVLGLREDLLVYGFA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCEEEECCCCEEEEEEC
EGTFRGRDLELGLRESRFRYGGLHVRVPYPGLGPAYGALAALAAAEVLGLSLEEVAERLA
CCCCCCCCEECCCHHCCCEECCEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHH
RLTLPPGRMETREVGGVVFVNDAYNANPLSVKAGLRWLAAQPGRKWAVLGEMRELGRESL
HCCCCCCCCCHHHCCCEEEEECCCCCCCEEHHHHHHHHHCCCCCCCHHHHHHHHHCHHHH
RLHLEVAEEAARLGLSPLYLGPHARHQAAFSGEAVESLEEALAWLKARVAPGDLVYLKAS
EEHHHHHHHHHHCCCCCEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEECC
RAVGLERIVELWDAPFSS
HHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]