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The map label for this gene is hpaG [H]

Identifier: 55980927

GI number: 55980927

Start: 903971

End: 904711

Strand: Direct

Name: hpaG [H]

Synonym: TTHA0958

Alternate gene names: 55980927

Gene position: 903971-904711 (Clockwise)

Preceding gene: 55980926

Following gene: 55980928

Centisome position: 48.87

GC content: 67.48

Gene sequence:

>741_bases
GTGAAGCTCGCCCGGTTTCTCGCCAAGGGAAGGGTCCACCAGGGGGTTTACCGGGAAGGCCTCCTCCTGGACGAGGCGGG
GGAGGCCCACAGGCCCGAGGACGTCACCTGGCTCCTCCCCTTCACCCCGGGGAAGATCCTGGGCGTGGCCTTGAACTACG
CCGATCACGCCGAGGAGCTCGGCCTCTCCCGCCCCGAGGAGCCCGCCCTCTTCTGGAAGCCCAACACGAGCCTCCTCCCC
CACAAGGGGGTGGTCCTCTACCCCAAGGGGGCGAGGTTCGTCCACTACGAGGTGGAGCTCGCCGTGGTGGTGGGCCGCCC
CATGAAGCGGGTGAGGGCCAAGGACGCCCTGGACTACGTCCTGGGCTACACCATCGCCAACGACCTCGTGGCCCGGGACT
ACGTGACCAACACCTTCCGCCCCCCCATCCGGGCCAAGGGGCGGGACACCTTCCTCCCCCTGGGGCCCTTCCTCGTGGTG
GAGGAGGTGGAAGACCCCCAGGACCTTTGGCTTCGGGCCTACGTGAACGGGGAGCTCAGGCAGGAGGGGCACACCTCGAG
GATGCTCTACTCCGTGGCGGAGCTTCTGGAGTTCATCTCCGAGTTCATGACCCTGGAGCCCTACGACGTCCTCCTCACCG
GCACCCCCAAGGGGATCAGCCAGGTGCGGCCCGGGGACGTGATGCGTTTGGAGATTGAGGGCCTTGGGGCCCTGGAGAAC
CCCATAGAGGAGGAGCCATGA

Upstream 100 bases:

>100_bases
AGTGGCGGCCCCCCTTGGGCCCCACCACCCCGGAGGTGGAGGAGAGGCTCAGGCGGATGGCCGAGCGCTACGGCCTCCTC
CCCAAGGAGAAGGAGGCGGC

Downstream 100 bases:

>100_bases
GGTACGCGGACCGGGTGGCCGGGATTTCCTGGGAGACGATTGAGGAGGTGCGCCGGCGCCTCAAGGAAAGGCCCGCCCTC
CACTTCATCGCCGGGGAGTT

Product: 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase/5-carboxymethyl-2-oxo-hex-3-ene-1,7-dioate decarboxylase

Products: NA

Alternate protein names: 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase; HHDD isomerase; 5-carboxymethyl-2-hydroxymuconate Delta-isomerase; 5-carboxymethyl-2-oxo-hex-3-ene-1,7-dioate decarboxylase; 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase; OPET decarboxylase [H]

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MKLARFLAKGRVHQGVYREGLLLDEAGEAHRPEDVTWLLPFTPGKILGVALNYADHAEELGLSRPEEPALFWKPNTSLLP
HKGVVLYPKGARFVHYEVELAVVVGRPMKRVRAKDALDYVLGYTIANDLVARDYVTNTFRPPIRAKGRDTFLPLGPFLVV
EEVEDPQDLWLRAYVNGELRQEGHTSRMLYSVAELLEFISEFMTLEPYDVLLTGTPKGISQVRPGDVMRLEIEGLGALEN
PIEEEP

Sequences:

>Translated_246_residues
MKLARFLAKGRVHQGVYREGLLLDEAGEAHRPEDVTWLLPFTPGKILGVALNYADHAEELGLSRPEEPALFWKPNTSLLP
HKGVVLYPKGARFVHYEVELAVVVGRPMKRVRAKDALDYVLGYTIANDLVARDYVTNTFRPPIRAKGRDTFLPLGPFLVV
EEVEDPQDLWLRAYVNGELRQEGHTSRMLYSVAELLEFISEFMTLEPYDVLLTGTPKGISQVRPGDVMRLEIEGLGALEN
PIEEEP
>Mature_246_residues
MKLARFLAKGRVHQGVYREGLLLDEAGEAHRPEDVTWLLPFTPGKILGVALNYADHAEELGLSRPEEPALFWKPNTSLLP
HKGVVLYPKGARFVHYEVELAVVVGRPMKRVRAKDALDYVLGYTIANDLVARDYVTNTFRPPIRAKGRDTFLPLGPFLVV
EEVEDPQDLWLRAYVNGELRQEGHTSRMLYSVAELLEFISEFMTLEPYDVLLTGTPKGISQVRPGDVMRLEIEGLGALEN
PIEEEP

Specific function: Decarboxylates OPET (5-oxo-pent-3-ene-1,2,5- tricarboxylic acid) into HHDD (2-hydroxy-hept-2,4-diene-1,7- dioate) and isomerizes it to OHED (2-oxo-hept-3-ene-1,7-dioate) [H]

COG id: COG0179

COG function: function code Q; 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAH family [H]

Homologues:

Organism=Homo sapiens, GI40786394, Length=255, Percent_Identity=38.0392156862745, Blast_Score=158, Evalue=5e-39,
Organism=Homo sapiens, GI156231349, Length=255, Percent_Identity=38.0392156862745, Blast_Score=158, Evalue=5e-39,
Organism=Homo sapiens, GI13654274, Length=203, Percent_Identity=38.423645320197, Blast_Score=142, Evalue=2e-34,
Organism=Homo sapiens, GI66348062, Length=194, Percent_Identity=39.6907216494845, Blast_Score=141, Evalue=4e-34,
Organism=Homo sapiens, GI215422413, Length=194, Percent_Identity=39.6907216494845, Blast_Score=141, Evalue=5e-34,
Organism=Escherichia coli, GI1787428, Length=201, Percent_Identity=36.81592039801, Blast_Score=132, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17557057, Length=189, Percent_Identity=39.1534391534392, Blast_Score=146, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6324161, Length=224, Percent_Identity=31.6964285714286, Blast_Score=118, Evalue=1e-27,
Organism=Drosophila melanogaster, GI28572127, Length=221, Percent_Identity=39.3665158371041, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI28571789, Length=204, Percent_Identity=39.7058823529412, Blast_Score=150, Evalue=9e-37,
Organism=Drosophila melanogaster, GI24663695, Length=209, Percent_Identity=36.3636363636364, Blast_Score=145, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234
- InterPro:   IPR012684
- InterPro:   IPR012686 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: =5.3.3.10; =4.1.1.68 [H]

Molecular weight: Translated: 27731; Mature: 27731

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLARFLAKGRVHQGVYREGLLLDEAGEAHRPEDVTWLLPFTPGKILGVALNYADHAEEL
CCHHHHHHHCCHHHHHHHCCEEEECCCCCCCCCCEEEEEECCCCCEEEEEECCHHHHHHH
GLSRPEEPALFWKPNTSLLPHKGVVLYPKGARFVHYEVELAVVVGRPMKRVRAKDALDYV
CCCCCCCCEEEECCCCCCCCCCCEEEEECCCEEEEEEEEEEEEECCCHHHHHHHHHHHHH
LGYTIANDLVARDYVTNTFRPPIRAKGRDTFLPLGPFLVVEEVEDPQDLWLRAYVNGELR
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCEEEEECCCCHHHHEEEEEECCHHH
QEGHTSRMLYSVAELLEFISEFMTLEPYDVLLTGTPKGISQVRPGDVMRLEIEGLGALEN
CCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHCCCCCEEEEEECCCCCCCC
PIEEEP
CCCCCC
>Mature Secondary Structure
MKLARFLAKGRVHQGVYREGLLLDEAGEAHRPEDVTWLLPFTPGKILGVALNYADHAEEL
CCHHHHHHHCCHHHHHHHCCEEEECCCCCCCCCCEEEEEECCCCCEEEEEECCHHHHHHH
GLSRPEEPALFWKPNTSLLPHKGVVLYPKGARFVHYEVELAVVVGRPMKRVRAKDALDYV
CCCCCCCCEEEECCCCCCCCCCCEEEEECCCEEEEEEEEEEEEECCCHHHHHHHHHHHHH
LGYTIANDLVARDYVTNTFRPPIRAKGRDTFLPLGPFLVVEEVEDPQDLWLRAYVNGELR
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCEEEEECCCCHHHHEEEEEECCHHH
QEGHTSRMLYSVAELLEFISEFMTLEPYDVLLTGTPKGISQVRPGDVMRLEIEGLGALEN
CCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHCCCCCEEEEEECCCCCCCC
PIEEEP
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA