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The map label for this gene is dapE [H]

Identifier: 55980478

GI number: 55980478

Start: 473807

End: 475105

Strand: Direct

Name: dapE [H]

Synonym: TTHA0509

Alternate gene names: 55980478

Gene position: 473807-475105 (Clockwise)

Preceding gene: 55980477

Following gene: 55980484

Centisome position: 25.61

GC content: 71.21

Gene sequence:

>1299_bases
GTGGACCTCACCGAGCTTTTTGCCTTTCTCTCCATCCCCTCCGTCTCCACCGACCCGGCCCGGCGGGAGGACGTGCGCCG
GGCGGCCCTTTGGCTTGAGGAGAGGCTTAAGGCCCGGGGCTTCCGCACCGAGCTCCACGAGACCCCCCTCCACCCCATCC
TCTACGCCGAGCGGTTCGTGGACGAGAAGGCCCCCACCGTCCTGGTCTACGGCCACTACGACGTCCAGCCCCCGGATCCC
CTGGAGCTTTGGGAGAGCCCGCCCTTCTCCCCGGTGGTGCGGGAGGGCCGGATCTACGCCCGGGGGGCCTCCGACGACAA
GGGGCAGCTTTTCGCCCACGTCCTCGCGGCGGAGGAGGCCCCGGTCAACCTGAAGTTCCTGGTGGAGGGGGAGGAGGAGA
TCGGAAGCCCCCACCTCCCCCCCTTCGTGCGGGCGAACCGGGAGAAGCTCCGGGCGGACGTGGTGCTCGTCTCCGACGGG
GCCATGTTCGCCCCCCACACCCCGACCCTCACCTACGGCCTCCGGGGGCTTTGCTACCTGGAGGTGCGGCTTAGGGGGGC
GAGGCGGGACCTCCACTCGGGGGCCTTCGGGGGCGTGGCCCCGAACCCCATCCAGGCCCTGGGGTGGCTCCTCGCCCGGC
TGAAGGACGAGGGGACGGGGAGGGTCCTGATCCCGGGCTTCTACGAGAGGGTGCGCCCCGTCCCCGAGGAGGAAAAGGCC
CTCTGGCCCTCCCTGGACGAGGAGGCCCTGAAGCGGGAGCTTGGGGTGGAGATCCTCCCCGGGGAGGAGGGCTACGCCCC
CCTGGAACGGCTTTGGGCCCGGCCCACCCTGGACCCGAACGGCATCTGGGGCGGCTACCAGGGAGAGGGCTCCAAGACGG
TGATCCCCGCCGAGGCGGGGATGAAGCTCTCCATGCGCCTGGTGCCGGACCAGGACCCGGAGGAGGTGGCGGACCAGGCC
GAGGCCTACCTGAAGGCCGTCTGCCCCCCCGGCTACGCCCTGGAGGTGCGGAGGCTCCACGGGGGCAGGCCCGTCCTCAC
CGACCCCTTTAGCCCGCCCATGCGCCTCATGGCCCGGGCCCTGGAGGAGGTCTGGGGAAGGCCCCCCGTCTACACCCGGG
AGGGCGGGACGATCCCCGTGGTGGCCGAGCTCAAGGAAACCCTGGGGGCGCCCATCGTCCTTCTGGGCCTGGGGCTTCCC
GACGACAACCTCCACGCCCCCAACGAGAAGCTGGACCTGGTGAACCTGGAGAAGGGGGTGGAGGTCATAAGGCGCTTCCA
CCGCCTCCTTGCGGGGTAG

Upstream 100 bases:

>100_bases
TCTTGGAGAAGGAGAAGGAGGAGGCCTTGGGCGGGGGCTGAGGGCCCCCCTCTGCGCTCCCCGGTTGCGGGAACGCGGTG
CGGGCGTTTAGGGTGTCCTC

Downstream 100 bases:

>100_bases
GCGCCCCGCCGCGGGGGGGTCTTTAGAACACCTTCCCCCCCAGGGGCACCTCCCGGTCCGGGGCGAGGAGGACCACCCGC
CCCGCCTCGTCGGGGACCCC

Product: N-acyl-L-amino acid amidohydrolase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MDLTELFAFLSIPSVSTDPARREDVRRAALWLEERLKARGFRTELHETPLHPILYAERFVDEKAPTVLVYGHYDVQPPDP
LELWESPPFSPVVREGRIYARGASDDKGQLFAHVLAAEEAPVNLKFLVEGEEEIGSPHLPPFVRANREKLRADVVLVSDG
AMFAPHTPTLTYGLRGLCYLEVRLRGARRDLHSGAFGGVAPNPIQALGWLLARLKDEGTGRVLIPGFYERVRPVPEEEKA
LWPSLDEEALKRELGVEILPGEEGYAPLERLWARPTLDPNGIWGGYQGEGSKTVIPAEAGMKLSMRLVPDQDPEEVADQA
EAYLKAVCPPGYALEVRRLHGGRPVLTDPFSPPMRLMARALEEVWGRPPVYTREGGTIPVVAELKETLGAPIVLLGLGLP
DDNLHAPNEKLDLVNLEKGVEVIRRFHRLLAG

Sequences:

>Translated_432_residues
MDLTELFAFLSIPSVSTDPARREDVRRAALWLEERLKARGFRTELHETPLHPILYAERFVDEKAPTVLVYGHYDVQPPDP
LELWESPPFSPVVREGRIYARGASDDKGQLFAHVLAAEEAPVNLKFLVEGEEEIGSPHLPPFVRANREKLRADVVLVSDG
AMFAPHTPTLTYGLRGLCYLEVRLRGARRDLHSGAFGGVAPNPIQALGWLLARLKDEGTGRVLIPGFYERVRPVPEEEKA
LWPSLDEEALKRELGVEILPGEEGYAPLERLWARPTLDPNGIWGGYQGEGSKTVIPAEAGMKLSMRLVPDQDPEEVADQA
EAYLKAVCPPGYALEVRRLHGGRPVLTDPFSPPMRLMARALEEVWGRPPVYTREGGTIPVVAELKETLGAPIVLLGLGLP
DDNLHAPNEKLDLVNLEKGVEVIRRFHRLLAG
>Mature_432_residues
MDLTELFAFLSIPSVSTDPARREDVRRAALWLEERLKARGFRTELHETPLHPILYAERFVDEKAPTVLVYGHYDVQPPDP
LELWESPPFSPVVREGRIYARGASDDKGQLFAHVLAAEEAPVNLKFLVEGEEEIGSPHLPPFVRANREKLRADVVLVSDG
AMFAPHTPTLTYGLRGLCYLEVRLRGARRDLHSGAFGGVAPNPIQALGWLLARLKDEGTGRVLIPGFYERVRPVPEEEKA
LWPSLDEEALKRELGVEILPGEEGYAPLERLWARPTLDPNGIWGGYQGEGSKTVIPAEAGMKLSMRLVPDQDPEEVADQA
EAYLKAVCPPGYALEVRRLHGGRPVLTDPFSPPMRLMARALEEVWGRPPVYTREGGTIPVVAELKETLGAPIVLLGLGLP
DDNLHAPNEKLDLVNLEKGVEVIRRFHRLLAG

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Homo sapiens, GI271398239, Length=450, Percent_Identity=36.4444444444444, Blast_Score=259, Evalue=4e-69,
Organism=Homo sapiens, GI21071039, Length=450, Percent_Identity=31.7777777777778, Blast_Score=208, Evalue=1e-53,
Organism=Homo sapiens, GI271398277, Length=317, Percent_Identity=37.5394321766561, Blast_Score=199, Evalue=5e-51,
Organism=Escherichia coli, GI1788816, Length=204, Percent_Identity=29.9019607843137, Blast_Score=69, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17563146, Length=438, Percent_Identity=36.0730593607306, Blast_Score=261, Evalue=7e-70,
Organism=Caenorhabditis elegans, GI193211059, Length=442, Percent_Identity=34.1628959276018, Blast_Score=254, Evalue=5e-68,
Organism=Saccharomyces cerevisiae, GI6321159, Length=454, Percent_Identity=33.920704845815, Blast_Score=236, Evalue=4e-63,
Organism=Saccharomyces cerevisiae, GI6319758, Length=450, Percent_Identity=29.1111111111111, Blast_Score=178, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24585880, Length=447, Percent_Identity=35.5704697986577, Blast_Score=249, Evalue=2e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 47985; Mature: 47985

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLTELFAFLSIPSVSTDPARREDVRRAALWLEERLKARGFRTELHETPLHPILYAERFV
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHH
DEKAPTVLVYGHYDVQPPDPLELWESPPFSPVVREGRIYARGASDDKGQLFAHVLAAEEA
CCCCCEEEEEECCCCCCCCHHHHCCCCCCCHHHHCCEEEEECCCCCHHHHHHHHHHHCCC
PVNLKFLVEGEEEIGSPHLPPFVRANREKLRADVVLVSDGAMFAPHTPTLTYGLRGLCYL
CCEEEEEEECHHHCCCCCCCHHHHCCHHHHEEEEEEEECCCEECCCCCCHHHCCCCEEEE
EVRLRGARRDLHSGAFGGVAPNPIQALGWLLARLKDEGTGRVLIPGFYERVRPVPEEEKA
EEEHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCHHHHCCCCCCHHHC
LWPSLDEEALKRELGVEILPGEEGYAPLERLWARPTLDPNGIWGGYQGEGSKTVIPAEAG
CCCCCCHHHHHHHCCCEEECCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCC
MKLSMRLVPDQDPEEVADQAEAYLKAVCPPGYALEVRRLHGGRPVLTDPFSPPMRLMARA
CEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEHHHCCCCCCCCCCCCCHHHHHHHH
LEEVWGRPPVYTREGGTIPVVAELKETLGAPIVLLGLGLPDDNLHAPNEKLDLVNLEKGV
HHHHHCCCCCEECCCCCEEHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEEEEHHHHH
EVIRRFHRLLAG
HHHHHHHHHHCC
>Mature Secondary Structure
MDLTELFAFLSIPSVSTDPARREDVRRAALWLEERLKARGFRTELHETPLHPILYAERFV
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHH
DEKAPTVLVYGHYDVQPPDPLELWESPPFSPVVREGRIYARGASDDKGQLFAHVLAAEEA
CCCCCEEEEEECCCCCCCCHHHHCCCCCCCHHHHCCEEEEECCCCCHHHHHHHHHHHCCC
PVNLKFLVEGEEEIGSPHLPPFVRANREKLRADVVLVSDGAMFAPHTPTLTYGLRGLCYL
CCEEEEEEECHHHCCCCCCCHHHHCCHHHHEEEEEEEECCCEECCCCCCHHHCCCCEEEE
EVRLRGARRDLHSGAFGGVAPNPIQALGWLLARLKDEGTGRVLIPGFYERVRPVPEEEKA
EEEHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCHHHHCCCCCCHHHC
LWPSLDEEALKRELGVEILPGEEGYAPLERLWARPTLDPNGIWGGYQGEGSKTVIPAEAG
CCCCCCHHHHHHHCCCEEECCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCC
MKLSMRLVPDQDPEEVADQAEAYLKAVCPPGYALEVRRLHGGRPVLTDPFSPPMRLMARA
CEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEHHHCCCCCCCCCCCCCHHHHHHHH
LEEVWGRPPVYTREGGTIPVVAELKETLGAPIVLLGLGLPDDNLHAPNEKLDLVNLEKGV
HHHHHCCCCCEECCCCCEEHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEEEEHHHHH
EVIRRFHRLLAG
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA