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The map label for this gene is mjaIM [H]

Identifier: 55980378

GI number: 55980378

Start: 388370

End: 389263

Strand: Direct

Name: mjaIM [H]

Synonym: TTHA0409

Alternate gene names: 55980378

Gene position: 388370-389263 (Clockwise)

Preceding gene: 55980377

Following gene: 55980379

Centisome position: 21.0

GC content: 66.0

Gene sequence:

>894_bases
ATGAGGTCCTTTCCAAAGGCTAAAGAGGCCTTCTCAGAGGGGGAAAAGGTCTCCTTTGGAGTTCACCGCCTCCACGTGGG
GGACGCCCGGGAGGTGCTGGCCTCCTTCCCCGAGGCCTCGGTCCACCTGGTGGTGACCTCCCCGCCCTACTGGACCCTGA
AGCGCTACGAGGACACCCCCGGCCAGCTCGGCCACATAGAGGACTACGAGGCCTTTCTGGACGAGCTGGACCGGGTGTGG
CGGGAGGTCTTCCGCCTCCTGGTCCCGGGCGGAAGGCTCGTCATCGTGGTGGGGGACGTGGCCGTGGCGAGGCGCCGCTT
TGGGCGCCACCTCGTCTTCCCCCTCCACGCCGACATCCAGGTGCGCTGCCGCAAGCTCGGGTTTGATAACCTCAACCCCA
TCATCTGGCACAAGCACACCAACGCCAGCCTCGAGGTGGAGGGGCGGGGCGTCTTCCTGGGCAAGCCCTACGAGCCCGGG
GCCATCATCAAGACGGAGATTGAGTACATCCTCATGCAAAGGAAGCCCGGGGGGTACCGCAAGCCCACCCAAGAGCAGCG
GGAGAAGAGCCGCCTTCCCAAGGAGGACTTTCACCGCTTCTTCCGGCAGATCTGGGACGACATCCCCGGGGAGAGCACCA
AGGACCACCCCGCCCCCTTCCCCCTGGAGCTCGCCGAGCGCCTGGTGCGGATGTTCAGCTTCGTGGGGGACGTGGTCTTA
GACCCCTTCGCCGGGACCGGGACCACCCTCATCGCCGCGGCGAGGTGGGGGAGGAGGGCTTTGGGCGTGGAGCTCGTGCC
GCGGTACGCTCAGCTGGCCAAAGAGCGCTTTGCCAGGGAAGTTCCTGGGTTTTCCCTGGAGGTCTTGGATGGAGCCACCC
ATCCTCGCCGTTAG

Upstream 100 bases:

>100_bases
AGCTTCTCGCCCCCGTGCCCCAGGACATGGCGGCGGCCTGGGAGGCCCTGGGGGGGAGGTGGCCGGAGGAGGTGGTGCGG
GAAGGGTATGCTGGGGCGCA

Downstream 100 bases:

>100_bases
GTTGAAAAGGCTTTTAGAAAACATGATACGGACCTCTTCCCAGGATAGCGGGCGCCAGGGGGTTTTGGCGGGGTATTTGG
AGGAACGTTTACGGGAGCGC

Product: modification methylase

Products: NA

Alternate protein names: M.MjaI; N-4 cytosine-specific methyltransferase MjaI [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MRSFPKAKEAFSEGEKVSFGVHRLHVGDAREVLASFPEASVHLVVTSPPYWTLKRYEDTPGQLGHIEDYEAFLDELDRVW
REVFRLLVPGGRLVIVVGDVAVARRRFGRHLVFPLHADIQVRCRKLGFDNLNPIIWHKHTNASLEVEGRGVFLGKPYEPG
AIIKTEIEYILMQRKPGGYRKPTQEQREKSRLPKEDFHRFFRQIWDDIPGESTKDHPAPFPLELAERLVRMFSFVGDVVL
DPFAGTGTTLIAAARWGRRALGVELVPRYAQLAKERFAREVPGFSLEVLDGATHPRR

Sequences:

>Translated_297_residues
MRSFPKAKEAFSEGEKVSFGVHRLHVGDAREVLASFPEASVHLVVTSPPYWTLKRYEDTPGQLGHIEDYEAFLDELDRVW
REVFRLLVPGGRLVIVVGDVAVARRRFGRHLVFPLHADIQVRCRKLGFDNLNPIIWHKHTNASLEVEGRGVFLGKPYEPG
AIIKTEIEYILMQRKPGGYRKPTQEQREKSRLPKEDFHRFFRQIWDDIPGESTKDHPAPFPLELAERLVRMFSFVGDVVL
DPFAGTGTTLIAAARWGRRALGVELVPRYAQLAKERFAREVPGFSLEVLDGATHPRR
>Mature_297_residues
MRSFPKAKEAFSEGEKVSFGVHRLHVGDAREVLASFPEASVHLVVTSPPYWTLKRYEDTPGQLGHIEDYEAFLDELDRVW
REVFRLLVPGGRLVIVVGDVAVARRRFGRHLVFPLHADIQVRCRKLGFDNLNPIIWHKHTNASLEVEGRGVFLGKPYEPG
AIIKTEIEYILMQRKPGGYRKPTQEQREKSRLPKEDFHRFFRQIWDDIPGESTKDHPAPFPLELAERLVRMFSFVGDVVL
DPFAGTGTTLIAAARWGRRALGVELVPRYAQLAKERFAREVPGFSLEVLDGATHPRR

Specific function: This methylase recognizes the double-stranded sequence CTAG, causes specific methylation on C-1 on both strands, and protects the DNA from cleavage by the MjaI endonuclease (Potential) [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=268, Percent_Identity=28.7313432835821, Blast_Score=71, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR017985
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.113 [H]

Molecular weight: Translated: 33968; Mature: 33968

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS00093 N4_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSFPKAKEAFSEGEKVSFGVHRLHVGDAREVLASFPEASVHLVVTSPPYWTLKRYEDTP
CCCCCCHHHHHCCCCEEEECEEEEECCCHHHHHHHCCCCEEEEEEECCCCEEEEECCCCC
GQLGHIEDYEAFLDELDRVWREVFRLLVPGGRLVIVVGDVAVARRRFGRHLVFPLHADIQ
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHCCEEEEEECCCHH
VRCRKLGFDNLNPIIWHKHTNASLEVEGRGVFLGKPYEPGAIIKTEIEYILMQRKPGGYR
HHHHHCCCCCCCCEEEEECCCCEEEECCCEEEECCCCCCCCEEHEEEEEEEEECCCCCCC
KPTQEQREKSRLPKEDFHRFFRQIWDDIPGESTKDHPAPFPLELAERLVRMFSFVGDVVL
CCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
DPFAGTGTTLIAAARWGRRALGVELVPRYAQLAKERFAREVPGFSLEVLDGATHPRR
CCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
>Mature Secondary Structure
MRSFPKAKEAFSEGEKVSFGVHRLHVGDAREVLASFPEASVHLVVTSPPYWTLKRYEDTP
CCCCCCHHHHHCCCCEEEECEEEEECCCHHHHHHHCCCCEEEEEEECCCCEEEEECCCCC
GQLGHIEDYEAFLDELDRVWREVFRLLVPGGRLVIVVGDVAVARRRFGRHLVFPLHADIQ
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHCCEEEEEECCCHH
VRCRKLGFDNLNPIIWHKHTNASLEVEGRGVFLGKPYEPGAIIKTEIEYILMQRKPGGYR
HHHHHCCCCCCCCEEEEECCCCEEEECCCEEEECCCCCCCCEEHEEEEEEEEECCCCCCC
KPTQEQREKSRLPKEDFHRFFRQIWDDIPGESTKDHPAPFPLELAERLVRMFSFVGDVVL
CCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
DPFAGTGTTLIAAARWGRRALGVELVPRYAQLAKERFAREVPGFSLEVLDGATHPRR
CCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]