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The map label for this gene is gatB [H]

Identifier: 55980335

GI number: 55980335

Start: 345749

End: 347158

Strand: Direct

Name: gatB [H]

Synonym: TTHA0366

Alternate gene names: 55980335

Gene position: 345749-347158 (Clockwise)

Preceding gene: 55980334

Following gene: 55980336

Centisome position: 18.69

GC content: 69.15

Gene sequence:

>1410_bases
ATGTACGAAGCCGTCATCGGCCTCGAGGTCCACCTGCACCTCAAGACCCGCACCAAGATGTTCTGCGGCTGCCGGGCGGA
CTACTTCGGGGCGGAGCCCAACACCCACACCTGCCCGGTCTGCCTAGGCCTCCCGGGGGCCCTCCCCGTGCCCAACCGGG
TGGCGGTGGAGCACGGCCTGAGGCTCGCCCTGGCCTTGGGGGCCGAGGTGCCGGAAAGGCTCGTCTTCCACCGCAAGAAC
TACTTCTACCCCGACCTCCCCAAGAACTACCAGATCAGCCAGTACGACCTCCCCTTGGGCCGGGGGGGAAGCCTGCCTTT
GGGGGAGCGAAGGGTGCGCATCAAGCGCCTCCACCTGGAGGAAGACGCGGGGAAAAGCCTCCACCTGGAGGGGCGGACCC
TCCTGGACCTCAACCGGGCGGGAAGCCCCCTCATTGAGCTCGTCACCGAGCCCGACCTCAAGACCCCCGAGGAGGCCAGG
CTCTTCCTTCAGCGCATCCAGGCCCTGGTCCAGACCCTGGGGATCTCCGACGCCTCCCCCGAAGAGGGCAAGCTCCGGGC
CGACGTCAACGTCTCCGTGCGGCGGGTGGGGGAGCCTTTGGGCACCAAGGTGGAGATCAAAAACCTCAACTCCTTCAAGA
GCGTGCAACGGGCCCTGGAGTACGAGATCCGCCGCCAGACGGAGATCCTAAGGCGGGGGGAGAAGGTGAAGCAGGCGACC
ATGGGCTTTGAGGAGGGGAGCGGCAAAACCTACCCCATGCGCACCAAGGAGGAAGAGGCCGACTACCGCTACTTCCCCGA
GCCCGACCTGCCCCCCGTGGCCATCCCCCGGGACTGGCTTGAGGAGGTGCGGCGAAGCCTTCCCGAGCTCCCCTGGGAGA
AGGAGGCCCGCTACCGCGCCCTAGGCATCAAGGAAAAGGACGCCGAGGTCCTGGCCTACACCCCCTCCCTCGCCCGCTTC
CTAGACCAGGCCCTTCCCTTGGGCCTCGCCTCCCCCCAGGCCCTGGCCAACTGGCTCCTCGCCGACGTGGCGGGGCTTCT
CCACGAGCGGGGCCTCCGCCTGGAGGAGACCCGGCTCTCCCCCGAGGGCCTCGCCCGGCTCGTGGGGCTCTTTGAGCGGG
GAGAGGTCACGAGCCGGGTGGCCAAAAGCCTCCTCCCCGAGGTCCTCGAGGGCCAGGACCCGGAGGCCCTGGTGCGGGAA
CGGGGGCTCAAGGTGGTGGCGGACGAGGGGGCGCTGAAGGCCTTGGTGGCCGAGGCCATCGCCGCCATGCCCGAGGCGGC
GGAAAGCGTCCGCCAAGGAAAGGTCAAGGCCCTGGACGCCCTCGTGGGCCAGGTGATGCGCAAGACCCGGGGCCAAGCCC
GGCCCGACCTGGTGCGCCGCCTCCTCCTCGAGGCCCTTGGGGTAGGATAG

Upstream 100 bases:

>100_bases
CTCATCACCTACGAGATGGGGCTCGCCAGGGCCGTGGACCCCAAGGAGTTCATGAGGCTCGCGGGCGCGCCCGAGGGCGC
CAGGCGGTAAACTTTCCCCG

Downstream 100 bases:

>100_bases
GGCCGATGCGCTACGAGGAGGCCGGGGTCCACATCGAGGCCAAGGCCGAGGCCCTAAGGCGGGCCAGGGAGGCCATCGCC
GCCACCTACACCCCCGAGGT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MYEAVIGLEVHLHLKTRTKMFCGCRADYFGAEPNTHTCPVCLGLPGALPVPNRVAVEHGLRLALALGAEVPERLVFHRKN
YFYPDLPKNYQISQYDLPLGRGGSLPLGERRVRIKRLHLEEDAGKSLHLEGRTLLDLNRAGSPLIELVTEPDLKTPEEAR
LFLQRIQALVQTLGISDASPEEGKLRADVNVSVRRVGEPLGTKVEIKNLNSFKSVQRALEYEIRRQTEILRRGEKVKQAT
MGFEEGSGKTYPMRTKEEEADYRYFPEPDLPPVAIPRDWLEEVRRSLPELPWEKEARYRALGIKEKDAEVLAYTPSLARF
LDQALPLGLASPQALANWLLADVAGLLHERGLRLEETRLSPEGLARLVGLFERGEVTSRVAKSLLPEVLEGQDPEALVRE
RGLKVVADEGALKALVAEAIAAMPEAAESVRQGKVKALDALVGQVMRKTRGQARPDLVRRLLLEALGVG

Sequences:

>Translated_469_residues
MYEAVIGLEVHLHLKTRTKMFCGCRADYFGAEPNTHTCPVCLGLPGALPVPNRVAVEHGLRLALALGAEVPERLVFHRKN
YFYPDLPKNYQISQYDLPLGRGGSLPLGERRVRIKRLHLEEDAGKSLHLEGRTLLDLNRAGSPLIELVTEPDLKTPEEAR
LFLQRIQALVQTLGISDASPEEGKLRADVNVSVRRVGEPLGTKVEIKNLNSFKSVQRALEYEIRRQTEILRRGEKVKQAT
MGFEEGSGKTYPMRTKEEEADYRYFPEPDLPPVAIPRDWLEEVRRSLPELPWEKEARYRALGIKEKDAEVLAYTPSLARF
LDQALPLGLASPQALANWLLADVAGLLHERGLRLEETRLSPEGLARLVGLFERGEVTSRVAKSLLPEVLEGQDPEALVRE
RGLKVVADEGALKALVAEAIAAMPEAAESVRQGKVKALDALVGQVMRKTRGQARPDLVRRLLLEALGVG
>Mature_469_residues
MYEAVIGLEVHLHLKTRTKMFCGCRADYFGAEPNTHTCPVCLGLPGALPVPNRVAVEHGLRLALALGAEVPERLVFHRKN
YFYPDLPKNYQISQYDLPLGRGGSLPLGERRVRIKRLHLEEDAGKSLHLEGRTLLDLNRAGSPLIELVTEPDLKTPEEAR
LFLQRIQALVQTLGISDASPEEGKLRADVNVSVRRVGEPLGTKVEIKNLNSFKSVQRALEYEIRRQTEILRRGEKVKQAT
MGFEEGSGKTYPMRTKEEEADYRYFPEPDLPPVAIPRDWLEEVRRSLPELPWEKEARYRALGIKEKDAEVLAYTPSLARF
LDQALPLGLASPQALANWLLADVAGLLHERGLRLEETRLSPEGLARLVGLFERGEVTSRVAKSLLPEVLEGQDPEALVRE
RGLKVVADEGALKALVAEAIAAMPEAAESVRQGKVKALDALVGQVMRKTRGQARPDLVRRLLLEALGVG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=496, Percent_Identity=35.6854838709677, Blast_Score=297, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI115534094, Length=344, Percent_Identity=36.3372093023256, Blast_Score=205, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6319391, Length=520, Percent_Identity=30.5769230769231, Blast_Score=214, Evalue=2e-56,
Organism=Drosophila melanogaster, GI45550808, Length=484, Percent_Identity=33.0578512396694, Blast_Score=246, Evalue=2e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: NA

Molecular weight: Translated: 52237; Mature: 52237

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYEAVIGLEVHLHLKTRTKMFCGCRADYFGAEPNTHTCPVCLGLPGALPVPNRVAVEHGL
CCCEEEEEEEEEEEEHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHCH
RLALALGAEVPERLVFHRKNYFYPDLPKNYQISQYDLPLGRGGSLPLGERRVRIKRLHLE
HHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHEEEC
EDAGKSLHLEGRTLLDLNRAGSPLIELVTEPDLKTPEEARLFLQRIQALVQTLGISDASP
CCCCCEEEECCCEEEECCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
EEGKLRADVNVSVRRVGEPLGTKVEIKNLNSFKSVQRALEYEIRRQTEILRRGEKVKQAT
CCCCEEECCCHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MGFEEGSGKTYPMRTKEEEADYRYFPEPDLPPVAIPRDWLEEVRRSLPELPWEKEARYRA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHH
LGIKEKDAEVLAYTPSLARFLDQALPLGLASPQALANWLLADVAGLLHERGLRLEETRLS
CCCCCCCCCEEEECHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHCCC
PEGLARLVGLFERGEVTSRVAKSLLPEVLEGQDPEALVRERGLKVVADEGALKALVAEAI
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEECCHHHHHHHHHHH
AAMPEAAESVRQGKVKALDALVGQVMRKTRGQARPDLVRRLLLEALGVG
HHCHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYEAVIGLEVHLHLKTRTKMFCGCRADYFGAEPNTHTCPVCLGLPGALPVPNRVAVEHGL
CCCEEEEEEEEEEEEHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHCH
RLALALGAEVPERLVFHRKNYFYPDLPKNYQISQYDLPLGRGGSLPLGERRVRIKRLHLE
HHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHEEEC
EDAGKSLHLEGRTLLDLNRAGSPLIELVTEPDLKTPEEARLFLQRIQALVQTLGISDASP
CCCCCEEEECCCEEEECCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
EEGKLRADVNVSVRRVGEPLGTKVEIKNLNSFKSVQRALEYEIRRQTEILRRGEKVKQAT
CCCCEEECCCHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MGFEEGSGKTYPMRTKEEEADYRYFPEPDLPPVAIPRDWLEEVRRSLPELPWEKEARYRA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHH
LGIKEKDAEVLAYTPSLARFLDQALPLGLASPQALANWLLADVAGLLHERGLRLEETRLS
CCCCCCCCCEEEECHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHCCC
PEGLARLVGLFERGEVTSRVAKSLLPEVLEGQDPEALVRERGLKVVADEGALKALVAEAI
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEECCHHHHHHHHHHH
AAMPEAAESVRQGKVKALDALVGQVMRKTRGQARPDLVRRLLLEALGVG
HHCHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA