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The map label for this gene is ispG [H]

Identifier: 55980274

GI number: 55980274

Start: 291802

End: 293022

Strand: Direct

Name: ispG [H]

Synonym: TTHA0305

Alternate gene names: 55980274

Gene position: 291802-293022 (Clockwise)

Preceding gene: 55980273

Following gene: 55980275

Centisome position: 15.78

GC content: 69.94

Gene sequence:

>1221_bases
ATGGAGGGGATGAGGCGACCTACCCCCACGGTCTATGTGGGCCGCGTCCCCATAGGGGGCGCCCACCCCATCGCCGTGCA
GTCCATGACCAACACCCCCACCCGGGACGTGGAGGCCACCACCGCCCAGGTCCTGGAGCTCCACCGGGCGGGAAGCGAGA
TCGTGCGCCTCACGGTGAACGACGAGGAGGCGGCGAAGGCCGTGCCCGAGATCAAGAGGAGGCTCCTCGCCGAGGGGGCG
GAGGTGCCCCTGGTGGGCGACTTCCACTTCAACGGGCACCTCCTCCTGCGCAAGTACCCAAAGATGGCCGAGGCCCTGGA
CAAGTTCCGCATCAACCCGGGCACCCTGGGGCGGGGGCGGCACAAGGACGAGCACTTCGCCGAGATGATCCGCATCGCCA
TGGACCTGGGGAAGCCCGTGAGGATCGGGGCCAACTGGGGAAGCCTAGACCCCGCCCTCCTCACCGAGCTCATGGACCGA
AACGCCAGACGCCCCGAGCCCAAAAGCGCCCACGAGGTGGTCCTCGAGGCCCTGGTGGAAAGCGCCGTGCGCGCTTATGA
AGCCGCCTTGGAGATGGGCCTCGGGGAGGACAAGCTCGTCCTCTCCGCCAAGGTCTCCAAGGCGCGCGACCTGGTGTGGG
TCTACCGGGAGCTCGCCCGCCGCACCCAAGCCCCCCTCCACCTGGGCCTCACCGAGGCGGGGATGGGGGTGAAGGGGATC
GTGGCCTCGGCCGCCGCCTTAGCCCCTCTCCTCCTGGAGGGCATCGGGGACACCATCCGGGTCTCCCTTACCCCGGCCCC
GGGCGAGCCCCGGACCAAGGAGGTGGAGGTGGCCCAGGAGATCCTCCAGGCCCTGGGGCTTCGCGCCTTCGCCCCCGAGG
TGACGAGCTGCCCCGGGTGCGGCCGCACCACCAGCACCTTCTTCCAGGAGCTCGCCGAGGAGGTCTCCCGCCGCCTCAAG
GAAAGGCTTCCCGAGTGGCGGGCCCGCTACCCCGGGGTGGAGGAGCTCAAGGTGGCGGTGATGGGCTGCGTGGTGAACGG
CCCCGGGGAGAGCAAGCACGCCCACATCGGCATCTCCCTCCCGGGGGCGGGGGAGGAGCCCAAGGCCCCGGTCTACGCCG
ACGGCAAGCTCCTCACCATCCTCAAGGGGGAGGGAATCGCCGAGGAGTTCCTTAGGCTCGTGGAGGACTACGTCAAGACG
CGCTTCGCCCCCAAGGCCTGA

Upstream 100 bases:

>100_bases
TCACCGGGGAGGTGGTCTACGTGGACGCGGGCTACCACATCATGGGGATGGAGCTGGAAGGGTAGGCCACCCCCCTTTTT
CCCACCGGGCCTCGGCTAGC

Downstream 100 bases:

>100_bases
TGGACCCCTTCCGCTTCCAGGTGGAGGCCCGCGCGGGCAGGGCCCGGGTGGGGAGGCTCTTCACCCCCCACGGGGCGGTG
GAGACCCCCCTCTTCATGCC

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MEGMRRPTPTVYVGRVPIGGAHPIAVQSMTNTPTRDVEATTAQVLELHRAGSEIVRLTVNDEEAAKAVPEIKRRLLAEGA
EVPLVGDFHFNGHLLLRKYPKMAEALDKFRINPGTLGRGRHKDEHFAEMIRIAMDLGKPVRIGANWGSLDPALLTELMDR
NARRPEPKSAHEVVLEALVESAVRAYEAALEMGLGEDKLVLSAKVSKARDLVWVYRELARRTQAPLHLGLTEAGMGVKGI
VASAAALAPLLLEGIGDTIRVSLTPAPGEPRTKEVEVAQEILQALGLRAFAPEVTSCPGCGRTTSTFFQELAEEVSRRLK
ERLPEWRARYPGVEELKVAVMGCVVNGPGESKHAHIGISLPGAGEEPKAPVYADGKLLTILKGEGIAEEFLRLVEDYVKT
RFAPKA

Sequences:

>Translated_406_residues
MEGMRRPTPTVYVGRVPIGGAHPIAVQSMTNTPTRDVEATTAQVLELHRAGSEIVRLTVNDEEAAKAVPEIKRRLLAEGA
EVPLVGDFHFNGHLLLRKYPKMAEALDKFRINPGTLGRGRHKDEHFAEMIRIAMDLGKPVRIGANWGSLDPALLTELMDR
NARRPEPKSAHEVVLEALVESAVRAYEAALEMGLGEDKLVLSAKVSKARDLVWVYRELARRTQAPLHLGLTEAGMGVKGI
VASAAALAPLLLEGIGDTIRVSLTPAPGEPRTKEVEVAQEILQALGLRAFAPEVTSCPGCGRTTSTFFQELAEEVSRRLK
ERLPEWRARYPGVEELKVAVMGCVVNGPGESKHAHIGISLPGAGEEPKAPVYADGKLLTILKGEGIAEEFLRLVEDYVKT
RFAPKA
>Mature_406_residues
MEGMRRPTPTVYVGRVPIGGAHPIAVQSMTNTPTRDVEATTAQVLELHRAGSEIVRLTVNDEEAAKAVPEIKRRLLAEGA
EVPLVGDFHFNGHLLLRKYPKMAEALDKFRINPGTLGRGRHKDEHFAEMIRIAMDLGKPVRIGANWGSLDPALLTELMDR
NARRPEPKSAHEVVLEALVESAVRAYEAALEMGLGEDKLVLSAKVSKARDLVWVYRELARRTQAPLHLGLTEAGMGVKGI
VASAAALAPLLLEGIGDTIRVSLTPAPGEPRTKEVEVAQEILQALGLRAFAPEVTSCPGCGRTTSTFFQELAEEVSRRLK
ERLPEWRARYPGVEELKVAVMGCVVNGPGESKHAHIGISLPGAGEEPKAPVYADGKLLTILKGEGIAEEFLRLVEDYVKT
RFAPKA

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=372, Percent_Identity=38.1720430107527, Blast_Score=206, Evalue=2e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 44172; Mature: 44172

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGMRRPTPTVYVGRVPIGGAHPIAVQSMTNTPTRDVEATTAQVLELHRAGSEIVRLTVN
CCCCCCCCCEEEEECCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEC
DEEAAKAVPEIKRRLLAEGAEVPLVGDFHFNGHLLLRKYPKMAEALDKFRINPGTLGRGR
CHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCC
HKDEHFAEMIRIAMDLGKPVRIGANWGSLDPALLTELMDRNARRPEPKSAHEVVLEALVE
CCHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
SAVRAYEAALEMGLGEDKLVLSAKVSKARDLVWVYRELARRTQAPLHLGLTEAGMGVKGI
HHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCEEECCHHCCCCHHHH
VASAAALAPLLLEGIGDTIRVSLTPAPGEPRTKEVEVAQEILQALGLRAFAPEVTSCPGC
HHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCHHCCCCCCCCCCC
GRTTSTFFQELAEEVSRRLKERLPEWRARYPGVEELKVAVMGCVVNGPGESKHAHIGISL
CCHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCHHHHHHHHHHEEECCCCCCCCEEEEEEC
PGAGEEPKAPVYADGKLLTILKGEGIAEEFLRLVEDYVKTRFAPKA
CCCCCCCCCCEEECCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MEGMRRPTPTVYVGRVPIGGAHPIAVQSMTNTPTRDVEATTAQVLELHRAGSEIVRLTVN
CCCCCCCCCEEEEECCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEC
DEEAAKAVPEIKRRLLAEGAEVPLVGDFHFNGHLLLRKYPKMAEALDKFRINPGTLGRGR
CHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCC
HKDEHFAEMIRIAMDLGKPVRIGANWGSLDPALLTELMDRNARRPEPKSAHEVVLEALVE
CCHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
SAVRAYEAALEMGLGEDKLVLSAKVSKARDLVWVYRELARRTQAPLHLGLTEAGMGVKGI
HHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCEEECCHHCCCCHHHH
VASAAALAPLLLEGIGDTIRVSLTPAPGEPRTKEVEVAQEILQALGLRAFAPEVTSCPGC
HHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCHHCCCCCCCCCCC
GRTTSTFFQELAEEVSRRLKERLPEWRARYPGVEELKVAVMGCVVNGPGESKHAHIGISL
CCHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCHHHHHHHHHHEEECCCCCCCCEEEEEEC
PGAGEEPKAPVYADGKLLTILKGEGIAEEFLRLVEDYVKTRFAPKA
CCCCCCCCCCEEECCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA