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The map label for this gene is tgt [H]

Identifier: 55980275

GI number: 55980275

Start: 293022

End: 294179

Strand: Direct

Name: tgt [H]

Synonym: TTHA0306

Alternate gene names: 55980275

Gene position: 293022-294179 (Clockwise)

Preceding gene: 55980274

Following gene: 55980276

Centisome position: 15.84

GC content: 71.42

Gene sequence:

>1158_bases
ATGGACCCCTTCCGCTTCCAGGTGGAGGCCCGCGCGGGCAGGGCCCGGGTGGGGAGGCTCTTCACCCCCCACGGGGCGGT
GGAGACCCCCCTCTTCATGCCCGTGGGCACGGCGGGCTCGGTGAAGGGCCTTATGCCCAAGGACCTCGAGGCCATCGGAA
GCCAGGTCCTCCTCACCAACACCTACCACCTCCTCCTCCGCCCGGGCCCCGAAAGGGTGAGGGCCCTCGGGGGGCTTCAC
GGCTTCGCGGGGTGGAAAGGCCCTTGGCTTACGGACTCCGGGGGCTTCCAGGTGATGAGCCTCGGACACATGCGCCGCAT
TGACGAGGAGGGGGTGGTCTTCCAAAGCCACCTGGACGGGCGCCTCATCAAGCTCACCCCGGAAAGGAGCATCGCCGTGC
AGGAGGCTTTGGGGGCCGACCTCATCATGGCCTTTGACGAGTGCCCCCCCTACCCCTCCCCCCGGGAGTACCTCGAGGCC
TCCCTGGAGCGCACCCTCCGCTGGCTGGAGCGGAGCCTCAAGGCCAAGACCCGGCCCGACCAGGCCCTCTTCGGCATCGC
CCAGGGGGGGACGGACCCCGAGCTCAGGCGCCGCTCCACCCTGGAGACGATCCGGTTTGACCTCCCGGGCTACGCCATCG
GGGGCCTGGCCGTGGGGGAGCCCAAGGAGGCCATGTTCGCCATGGTGGAGCTCTCCACCCGCCTCCTCCCCGAGGACCGC
CCCCGCTACCTCATGGGGGTCGGCCACCCCGAGGACCTGGTGGCGGCCATGGGGCTTGGGGTGGACCTCTTTGACTGCGT
CTACCCCACCCGCACGGGCCGCTTCGGGAGCGCCCTGGTGCCGGAGGGGCGGCTCAACCTGAAAAACGCCCGCTTCCTCG
AGGACAGGCGCCCCCTGGAGGAGGGGTGCGACTGCTACACCTGCCAGACCTTTGGCCGCGCCTACCTCGCCCACCTGGTG
CGCGCCGGGGAGATGCTCGGGGGGATCCTCCTCTCCCTCCACAACCTGCGCCACCTCCACCGCCTCACCGAGGCCGCCCG
GCAGGCCATCCGCGAGGGGCGTTACGGGGACTTCGCCCGGGAGTTCGCCAGGAGGCGCTTCGGCCGGGAGGTGCCCCCCT
GGTTCCGCGAGGCCCTGGCCGCCGGGGGCCACGGGTAA

Upstream 100 bases:

>100_bases
ACGGCAAGCTCCTCACCATCCTCAAGGGGGAGGGAATCGCCGAGGAGTTCCTTAGGCTCGTGGAGGACTACGTCAAGACG
CGCTTCGCCCCCAAGGCCTG

Downstream 100 bases:

>100_bases
CACCACCCCATGCCGGCCTGCGCCGGCATGGGGCCCCGGCAAAAGGCGCCTGGTGGACCGAAGAGGGGATAAAATCTCCC
CGGTATGACGCCAAGGCATC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MDPFRFQVEARAGRARVGRLFTPHGAVETPLFMPVGTAGSVKGLMPKDLEAIGSQVLLTNTYHLLLRPGPERVRALGGLH
GFAGWKGPWLTDSGGFQVMSLGHMRRIDEEGVVFQSHLDGRLIKLTPERSIAVQEALGADLIMAFDECPPYPSPREYLEA
SLERTLRWLERSLKAKTRPDQALFGIAQGGTDPELRRRSTLETIRFDLPGYAIGGLAVGEPKEAMFAMVELSTRLLPEDR
PRYLMGVGHPEDLVAAMGLGVDLFDCVYPTRTGRFGSALVPEGRLNLKNARFLEDRRPLEEGCDCYTCQTFGRAYLAHLV
RAGEMLGGILLSLHNLRHLHRLTEAARQAIREGRYGDFAREFARRRFGREVPPWFREALAAGGHG

Sequences:

>Translated_385_residues
MDPFRFQVEARAGRARVGRLFTPHGAVETPLFMPVGTAGSVKGLMPKDLEAIGSQVLLTNTYHLLLRPGPERVRALGGLH
GFAGWKGPWLTDSGGFQVMSLGHMRRIDEEGVVFQSHLDGRLIKLTPERSIAVQEALGADLIMAFDECPPYPSPREYLEA
SLERTLRWLERSLKAKTRPDQALFGIAQGGTDPELRRRSTLETIRFDLPGYAIGGLAVGEPKEAMFAMVELSTRLLPEDR
PRYLMGVGHPEDLVAAMGLGVDLFDCVYPTRTGRFGSALVPEGRLNLKNARFLEDRRPLEEGCDCYTCQTFGRAYLAHLV
RAGEMLGGILLSLHNLRHLHRLTEAARQAIREGRYGDFAREFARRRFGREVPPWFREALAAGGHG
>Mature_385_residues
MDPFRFQVEARAGRARVGRLFTPHGAVETPLFMPVGTAGSVKGLMPKDLEAIGSQVLLTNTYHLLLRPGPERVRALGGLH
GFAGWKGPWLTDSGGFQVMSLGHMRRIDEEGVVFQSHLDGRLIKLTPERSIAVQEALGADLIMAFDECPPYPSPREYLEA
SLERTLRWLERSLKAKTRPDQALFGIAQGGTDPELRRRSTLETIRFDLPGYAIGGLAVGEPKEAMFAMVELSTRLLPEDR
PRYLMGVGHPEDLVAAMGLGVDLFDCVYPTRTGRFGSALVPEGRLNLKNARFLEDRRPLEEGCDCYTCQTFGRAYLAHLV
RAGEMLGGILLSLHNLRHLHRLTEAARQAIREGRYGDFAREFARRRFGREVPPWFREALAAGGHG

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=383, Percent_Identity=46.2140992167102, Blast_Score=348, Evalue=4e-96,
Organism=Homo sapiens, GI13375872, Length=295, Percent_Identity=26.7796610169492, Blast_Score=111, Evalue=1e-24,
Organism=Escherichia coli, GI1786607, Length=375, Percent_Identity=49.6, Blast_Score=370, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17544680, Length=397, Percent_Identity=43.8287153652393, Blast_Score=332, Evalue=2e-91,
Organism=Caenorhabditis elegans, GI25146502, Length=378, Percent_Identity=22.2222222222222, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI19920510, Length=380, Percent_Identity=45.2631578947368, Blast_Score=345, Evalue=3e-95,
Organism=Drosophila melanogaster, GI21355311, Length=266, Percent_Identity=24.812030075188, Blast_Score=82, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 42827; Mature: 42827

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPFRFQVEARAGRARVGRLFTPHGAVETPLFMPVGTAGSVKGLMPKDLEAIGSQVLLTN
CCCCEEEEECCCCCHHHCCEECCCCCCCCCCEEECCCCCCCCCCCCHHHHHHCCEEEEEC
TYHLLLRPGPERVRALGGLHGFAGWKGPWLTDSGGFQVMSLGHMRRIDEEGVVFQSHLDG
CEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECHHHHHCCCCCCEEEECCCC
RLIKLTPERSIAVQEALGADLIMAFDECPPYPSPREYLEASLERTLRWLERSLKAKTRPD
EEEEECCCHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
QALFGIAQGGTDPELRRRSTLETIRFDLPGYAIGGLAVGEPKEAMFAMVELSTRLLPEDR
HHHEEHHCCCCCHHHHHHHHHHHHHCCCCCCEECCEECCCCHHHHHHHHHHHHHCCCCCC
PRYLMGVGHPEDLVAAMGLGVDLFDCVYPTRTGRFGSALVPEGRLNLKNARFLEDRRPLE
CCEEEECCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCHH
EGCDCYTCQTFGRAYLAHLVRAGEMLGGILLSLHNLRHLHRLTEAARQAIREGRYGDFAR
HCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
EFARRRFGREVPPWFREALAAGGHG
HHHHHHHCCCCCHHHHHHHHCCCCC
>Mature Secondary Structure
MDPFRFQVEARAGRARVGRLFTPHGAVETPLFMPVGTAGSVKGLMPKDLEAIGSQVLLTN
CCCCEEEEECCCCCHHHCCEECCCCCCCCCCEEECCCCCCCCCCCCHHHHHHCCEEEEEC
TYHLLLRPGPERVRALGGLHGFAGWKGPWLTDSGGFQVMSLGHMRRIDEEGVVFQSHLDG
CEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECHHHHHCCCCCCEEEECCCC
RLIKLTPERSIAVQEALGADLIMAFDECPPYPSPREYLEASLERTLRWLERSLKAKTRPD
EEEEECCCHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
QALFGIAQGGTDPELRRRSTLETIRFDLPGYAIGGLAVGEPKEAMFAMVELSTRLLPEDR
HHHEEHHCCCCCHHHHHHHHHHHHHCCCCCCEECCEECCCCHHHHHHHHHHHHHCCCCCC
PRYLMGVGHPEDLVAAMGLGVDLFDCVYPTRTGRFGSALVPEGRLNLKNARFLEDRRPLE
CCEEEECCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCHH
EGCDCYTCQTFGRAYLAHLVRAGEMLGGILLSLHNLRHLHRLTEAARQAIREGRYGDFAR
HCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
EFARRRFGREVPPWFREALAAGGHG
HHHHHHHCCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA