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The map label for this gene is lpdG [H]

Identifier: 55980256

GI number: 55980256

Start: 277724

End: 279091

Strand: Reverse

Name: lpdG [H]

Synonym: TTHA0287

Alternate gene names: 55980256

Gene position: 279091-277724 (Counterclockwise)

Preceding gene: 55980257

Following gene: 55980253

Centisome position: 15.09

GC content: 69.52

Gene sequence:

>1368_bases
GTGTACGACCTCCTGGTGATCGGCGCCGGGCCCGGGGGGTACGTGGCCGCCATCCGGGCGGCCCAGCTCGGGATGAAGGT
GGGGGTGGTGGAGAAGGAGAAGGCCCTAGGGGGGACCTGCCTCAGGGTGGGGTGCATCCCCTCCAAGGCCCTCCTGGAGA
CCACCGAACGCATCTACGAGGCGAAGAAGGGCCTCCTCGGGGCCAAGGTGAAGGGGGTGGAGCTGGACCTTCCCGCCCTC
ATGGCCCACAAGGACAAGGTGGTCCAGGCCAACACCCAAGGGGTGGAGTTCCTCTTCAAAAAGAACGGCATCGCCCGCCA
CCAGGGCACGGCCCGCTTCCTCTCCGAGCGCAAGGTTTTGGTGGAGGAGACGGGGGAGGAGCTGGAGGCCCGCTACATCC
TCATCGCCACGGGAAGCGCCCCCCTCATCCCCCCTTGGGCCCAGGTGGACTACGAGCGGGTGGTGACCTCCACCGAGGCC
CTTTCCTTCCCCGAGGTGCCGAAGAGGCTCATCGTGGTGGGGGGCGGGGTGATCGGGCTGGAGCTCGGGGTGGTCTGGCA
CCGCCTGGGGGCGGAGGTCATCGTCCTGGAGTACATGGACCGCATCCTCCCCACCATGGACCTCGAGGTCTCCCGGGCGG
CGGAGAGGGTCTTCAAGAAGCAGGGCCTCACGATCCGCACCGGGGTGCGGGTCACTGCCGTGGTGCCTGAGGCCAAGGGG
GCCCGGGTGGAGCTTGAGGGGGGCGAGGTCTTGGAGGCGGATCGGGTCCTCGTGGCCGTGGGCCGGAGGCCCTACACCGA
GGGGCTTTCCCTGGAGAACGCCGGGCTTTCCACGGACGAGCGGGGGCGGATCCCCGTGGACGAGCACCTGAGGACCCGCG
TTCCCCACATCTACGCCATCGGGGACGTGGTGCGGGGTCCCATGCTCGCCCACAAGGCGAGCGAGGAAGGGATCGCCGCC
GTGGAGCACATGGTCCGGGGCTTCGGCCACGTGGACTACCAGGCCATCCCCAGCGTGGTCTACACCCACCCCGAGATCGC
CGCCGTGGGCTACACCGAGGAAGAGCTGAAGGCGCAGGGCATCCCCTACAAGGTGGGGAAGTTCCCCTACTCCGCCTCGG
GCCGCGCCCGGGCCATGGGGGAGACGGAGGGGTTTATTAAGGTCCTCGCCCACGCCAAGACGGACCGCATCCTCGGGGTG
CACGGGATCGGGGCCCGGGTGGGGGACGTCCTGGCCGAGGCCGCCTTGGCCCTCTTCTTCAAGGCGAGCGCCGAGGACTT
GGGCCGCGCCCCTCACGCCCACCCCTCCCTCTCCGAGATCCTCAAGGAGGCGGCGCTGGCGGCGTGGGAGCGGCCGATTC
ACCTGTAG

Upstream 100 bases:

>100_bases
TACGACCACCGCATCGTGGACGGACGGGAGGCGGTCACCTTCCTCCGCCGGGTGAAGGAGCTCATTGAGAACCCGGCGCG
GCTCCTTTTGGAGGTGTAGC

Downstream 100 bases:

>100_bases
GCGAAACTTGACCACCCCCCGGGGAAGCCCGGGGGGTGGGTGCTTACGGACCTTAGTTGGTCTTCTGGAGCTCGAGGCGG
TACTTGTTGAAGGGGCTGTC

Product: 2-oxoglutarate dehydrogenase E3 component (dihydrolipoamide dehydrogenase)

Products: NA

Alternate protein names: E3 component of 2-oxoglutarate dehydrogenase complex; Glycine oxidation system L-factor; LPD-GLC [H]

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MYDLLVIGAGPGGYVAAIRAAQLGMKVGVVEKEKALGGTCLRVGCIPSKALLETTERIYEAKKGLLGAKVKGVELDLPAL
MAHKDKVVQANTQGVEFLFKKNGIARHQGTARFLSERKVLVEETGEELEARYILIATGSAPLIPPWAQVDYERVVTSTEA
LSFPEVPKRLIVVGGGVIGLELGVVWHRLGAEVIVLEYMDRILPTMDLEVSRAAERVFKKQGLTIRTGVRVTAVVPEAKG
ARVELEGGEVLEADRVLVAVGRRPYTEGLSLENAGLSTDERGRIPVDEHLRTRVPHIYAIGDVVRGPMLAHKASEEGIAA
VEHMVRGFGHVDYQAIPSVVYTHPEIAAVGYTEEELKAQGIPYKVGKFPYSASGRARAMGETEGFIKVLAHAKTDRILGV
HGIGARVGDVLAEAALALFFKASAEDLGRAPHAHPSLSEILKEAALAAWERPIHL

Sequences:

>Translated_455_residues
MYDLLVIGAGPGGYVAAIRAAQLGMKVGVVEKEKALGGTCLRVGCIPSKALLETTERIYEAKKGLLGAKVKGVELDLPAL
MAHKDKVVQANTQGVEFLFKKNGIARHQGTARFLSERKVLVEETGEELEARYILIATGSAPLIPPWAQVDYERVVTSTEA
LSFPEVPKRLIVVGGGVIGLELGVVWHRLGAEVIVLEYMDRILPTMDLEVSRAAERVFKKQGLTIRTGVRVTAVVPEAKG
ARVELEGGEVLEADRVLVAVGRRPYTEGLSLENAGLSTDERGRIPVDEHLRTRVPHIYAIGDVVRGPMLAHKASEEGIAA
VEHMVRGFGHVDYQAIPSVVYTHPEIAAVGYTEEELKAQGIPYKVGKFPYSASGRARAMGETEGFIKVLAHAKTDRILGV
HGIGARVGDVLAEAALALFFKASAEDLGRAPHAHPSLSEILKEAALAAWERPIHL
>Mature_455_residues
MYDLLVIGAGPGGYVAAIRAAQLGMKVGVVEKEKALGGTCLRVGCIPSKALLETTERIYEAKKGLLGAKVKGVELDLPAL
MAHKDKVVQANTQGVEFLFKKNGIARHQGTARFLSERKVLVEETGEELEARYILIATGSAPLIPPWAQVDYERVVTSTEA
LSFPEVPKRLIVVGGGVIGLELGVVWHRLGAEVIVLEYMDRILPTMDLEVSRAAERVFKKQGLTIRTGVRVTAVVPEAKG
ARVELEGGEVLEADRVLVAVGRRPYTEGLSLENAGLSTDERGRIPVDEHLRTRVPHIYAIGDVVRGPMLAHKASEEGIAA
VEHMVRGFGHVDYQAIPSVVYTHPEIAAVGYTEEELKAQGIPYKVGKFPYSASGRARAMGETEGFIKVLAHAKTDRILGV
HGIGARVGDVLAEAALALFFKASAEDLGRAPHAHPSLSEILKEAALAAWERPIHL

Specific function: Also acts in the glycine cleavage system [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=459, Percent_Identity=46.4052287581699, Blast_Score=412, Evalue=1e-115,
Organism=Homo sapiens, GI50301238, Length=458, Percent_Identity=30.3493449781659, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI22035672, Length=480, Percent_Identity=29.7916666666667, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI291045266, Length=477, Percent_Identity=26.2054507337526, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI33519430, Length=480, Percent_Identity=26.25, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI33519428, Length=480, Percent_Identity=26.25, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI33519426, Length=480, Percent_Identity=26.25, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI148277065, Length=480, Percent_Identity=26.25, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI148277071, Length=480, Percent_Identity=26.25, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI291045268, Length=466, Percent_Identity=25.1072961373391, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1786307, Length=453, Percent_Identity=40.3973509933775, Blast_Score=300, Evalue=1e-82,
Organism=Escherichia coli, GI87082354, Length=459, Percent_Identity=32.244008714597, Blast_Score=241, Evalue=1e-64,
Organism=Escherichia coli, GI1789915, Length=431, Percent_Identity=30.8584686774942, Blast_Score=175, Evalue=5e-45,
Organism=Escherichia coli, GI87081717, Length=459, Percent_Identity=28.7581699346405, Blast_Score=161, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI32565766, Length=457, Percent_Identity=48.7964989059081, Blast_Score=430, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17557007, Length=468, Percent_Identity=28.2051282051282, Blast_Score=144, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI71983429, Length=362, Percent_Identity=30.1104972375691, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71983419, Length=362, Percent_Identity=30.1104972375691, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71982272, Length=477, Percent_Identity=25.3668763102725, Blast_Score=101, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17559934, Length=224, Percent_Identity=29.0178571428571, Blast_Score=78, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6321091, Length=475, Percent_Identity=48, Blast_Score=417, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6325240, Length=472, Percent_Identity=33.2627118644068, Blast_Score=247, Evalue=2e-66,
Organism=Saccharomyces cerevisiae, GI6325166, Length=463, Percent_Identity=28.2937365010799, Blast_Score=148, Evalue=2e-36,
Organism=Drosophila melanogaster, GI21358499, Length=466, Percent_Identity=49.7854077253219, Blast_Score=441, Evalue=1e-124,
Organism=Drosophila melanogaster, GI17737741, Length=480, Percent_Identity=28.75, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24640551, Length=483, Percent_Identity=27.9503105590062, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24640553, Length=484, Percent_Identity=27.6859504132231, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24640549, Length=484, Percent_Identity=27.6859504132231, Blast_Score=110, Evalue=1e-24,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 49059; Mature: 49059

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDLLVIGAGPGGYVAAIRAAQLGMKVGVVEKEKALGGTCLRVGCIPSKALLETTERIYE
CCEEEEEECCCCCHHHHHHHHHHCCEECCEEHHHHCCCEEEEEECCCCHHHHHHHHHHHH
AKKGLLGAKVKGVELDLPALMAHKDKVVQANTQGVEFLFKKNGIARHQGTARFLSERKVL
HHCCCCCCEECCEEECCHHHHHCCCCEEECCCCCEEEEEECCCCCCCCCHHHHHHHCHHH
VEETGEELEARYILIATGSAPLIPPWAQVDYERVVTSTEALSFPEVPKRLIVVGGGVIGL
HHHCCCCCCEEEEEEECCCCCCCCCHHHCCHHHHHHHHHHCCCCCCCCEEEEECCCCEEH
ELGVVWHRLGAEVIVLEYMDRILPTMDLEVSRAAERVFKKQGLTIRTGVRVTAVVPEAKG
HHHHHHHHHCCCEEHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEECCCEEEEEECCCCC
ARVELEGGEVLEADRVLVAVGRRPYTEGLSLENAGLSTDERGRIPVDEHLRTRVPHIYAI
CEEEECCCCEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEE
GDVVRGPMLAHKASEEGIAAVEHMVRGFGHVDYQAIPSVVYTHPEIAAVGYTEEELKAQG
HHHHCCCHHHHCCCCCHHHHHHHHHHHCCCCCHHHHCHHEECCCCEEEECCCHHHHHHCC
IPYKVGKFPYSASGRARAMGETEGFIKVLAHAKTDRILGVHGIGARVGDVLAEAALALFF
CCEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHH
KASAEDLGRAPHAHPSLSEILKEAALAAWERPIHL
HCCHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MYDLLVIGAGPGGYVAAIRAAQLGMKVGVVEKEKALGGTCLRVGCIPSKALLETTERIYE
CCEEEEEECCCCCHHHHHHHHHHCCEECCEEHHHHCCCEEEEEECCCCHHHHHHHHHHHH
AKKGLLGAKVKGVELDLPALMAHKDKVVQANTQGVEFLFKKNGIARHQGTARFLSERKVL
HHCCCCCCEECCEEECCHHHHHCCCCEEECCCCCEEEEEECCCCCCCCCHHHHHHHCHHH
VEETGEELEARYILIATGSAPLIPPWAQVDYERVVTSTEALSFPEVPKRLIVVGGGVIGL
HHHCCCCCCEEEEEEECCCCCCCCCHHHCCHHHHHHHHHHCCCCCCCCEEEEECCCCEEH
ELGVVWHRLGAEVIVLEYMDRILPTMDLEVSRAAERVFKKQGLTIRTGVRVTAVVPEAKG
HHHHHHHHHCCCEEHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEECCCEEEEEECCCCC
ARVELEGGEVLEADRVLVAVGRRPYTEGLSLENAGLSTDERGRIPVDEHLRTRVPHIYAI
CEEEECCCCEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEE
GDVVRGPMLAHKASEEGIAAVEHMVRGFGHVDYQAIPSVVYTHPEIAAVGYTEEELKAQG
HHHHCCCHHHHCCCCCHHHHHHHHHHHCCCCCHHHHCHHEECCCCEEEECCCHHHHHHCC
IPYKVGKFPYSASGRARAMGETEGFIKVLAHAKTDRILGVHGIGARVGDVLAEAALALFF
CCEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHH
KASAEDLGRAPHAHPSLSEILKEAALAAWERPIHL
HCCHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]