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The map label for this gene is sucB [H]

Identifier: 55980257

GI number: 55980257

Start: 279093

End: 280313

Strand: Reverse

Name: sucB [H]

Synonym: TTHA0288

Alternate gene names: 55980257

Gene position: 280313-279093 (Counterclockwise)

Preceding gene: 55980258

Following gene: 55980256

Centisome position: 15.15

GC content: 68.39

Gene sequence:

>1221_bases
GTGCAGGAACTCAAGGTGCCCTCCGTGGGCGAAAGCATCGTGGAAGTGGAGATCGGCGCTTGGCTTAAGGGAGAAGGGGA
GAGCTTCGCCCAGGACGAGCCCTTGGTGGAGCTCATCACCGACAAGGCCACCCTAGAGCTCCCCGCCCCCTTCGCCGGGA
CCCTGAAGCAGATCCTGAAGAGGACCGGGGAGACCGCCCGGGTGGGGGAGGCCATCGCCCTCCTGGAGGAGGGGAGGGCG
GAGGCCGCGCCCAAGGCCCAGGCGCCCGCCGAGGCCCCCAAGGAGCCCTCCCCAGAGCCTTTGGCCATGCCCGCCGCGGA
GCGCCTCATGCAGGAAAAGGGCGTCTCCCCGGCAGAGGTTCAGGGGACGGGCCTCGGCGGGCGGATCCTTAAGGAGGACG
TGATGCGCCACCTGGAGGAAAGGGCCCCCGCCAAACCCCAGGAGGCCCCGCCGCCTCCTCCCCCCTCCCGCCCCGCGCCC
CCGCCCTCCACCCCCCCGCAGCCTCCCGCCGACAAGCCCTGGCGGGTGAGCGAGGCCGTGCCCATGACGCCCTTGCGCCG
CCGGATCGCCGAGCGCCTCCTCATGGTCCGCCAGACCACGGCCATGCTCACCACCTTCAACGAGGCGGACATGTCGGCGG
TGATCGCCCTCAGGAAGGAGTTCGGCGAGGCCTTCCAGAAGAAGTACGGGGTCAAGCTCGGCTTCATGAGCTTCTTCGTC
AAGGCCGTGGTCCAGGCCCTCAAGGAGATCCCCGAGCTCAACGCCGAGATCCGGGACAACCACATCGTCTACCACCGCTA
CTACGACATCGGCATCGCCGTGGGCGGAGGCGAGGGCCTGGTGGTCCCCGTGATCCGCGACGCCGACCGCCTCTCCTTCG
CCGAGATTGAGCAAAAGATCGCCGACTTCGCCGAAAGGGCCCGCACCAAGAAGCTCAAGCCCGAGGAGCTCATGGGGGGC
ACCTTCACCATCACCAACGGGGGGATCTACGGCTCCCTGAACTCCACCCCCCTCCTCAACCCGCCCCAGGTGGGGATCCT
GGGCATGCACGCCATCCAGGAGCGCCCCGTGGTTCGGGACGGCCAGGTGGTCATAAGGCCCATGATGTACCTCGCCCTCT
CCTACGACCACCGCATCGTGGACGGACGGGAGGCGGTCACCTTCCTCCGCCGGGTGAAGGAGCTCATTGAGAACCCGGCG
CGGCTCCTTTTGGAGGTGTAG

Upstream 100 bases:

>100_bases
GCCCGGCCCGAGTCCCCGAGCCCCGCCGTGGGTTCCTCCAAGGTCCACAAGCTGGAGCAGGAGGAGCTGCTGGAGGAAGC
GTTCAGGTAAGGAGGTAGCC

Downstream 100 bases:

>100_bases
CGTGTACGACCTCCTGGTGATCGGCGCCGGGCCCGGGGGGTACGTGGCCGCCATCCGGGCGGCCCAGCTCGGGATGAAGG
TGGGGGTGGTGGAGAAGGAG

Product: 2-oxoglutarate dehydrogenase E2 component (dihydrolipoamide succinyltransferase)

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MQELKVPSVGESIVEVEIGAWLKGEGESFAQDEPLVELITDKATLELPAPFAGTLKQILKRTGETARVGEAIALLEEGRA
EAAPKAQAPAEAPKEPSPEPLAMPAAERLMQEKGVSPAEVQGTGLGGRILKEDVMRHLEERAPAKPQEAPPPPPPSRPAP
PPSTPPQPPADKPWRVSEAVPMTPLRRRIAERLLMVRQTTAMLTTFNEADMSAVIALRKEFGEAFQKKYGVKLGFMSFFV
KAVVQALKEIPELNAEIRDNHIVYHRYYDIGIAVGGGEGLVVPVIRDADRLSFAEIEQKIADFAERARTKKLKPEELMGG
TFTITNGGIYGSLNSTPLLNPPQVGILGMHAIQERPVVRDGQVVIRPMMYLALSYDHRIVDGREAVTFLRRVKELIENPA
RLLLEV

Sequences:

>Translated_406_residues
MQELKVPSVGESIVEVEIGAWLKGEGESFAQDEPLVELITDKATLELPAPFAGTLKQILKRTGETARVGEAIALLEEGRA
EAAPKAQAPAEAPKEPSPEPLAMPAAERLMQEKGVSPAEVQGTGLGGRILKEDVMRHLEERAPAKPQEAPPPPPPSRPAP
PPSTPPQPPADKPWRVSEAVPMTPLRRRIAERLLMVRQTTAMLTTFNEADMSAVIALRKEFGEAFQKKYGVKLGFMSFFV
KAVVQALKEIPELNAEIRDNHIVYHRYYDIGIAVGGGEGLVVPVIRDADRLSFAEIEQKIADFAERARTKKLKPEELMGG
TFTITNGGIYGSLNSTPLLNPPQVGILGMHAIQERPVVRDGQVVIRPMMYLALSYDHRIVDGREAVTFLRRVKELIENPA
RLLLEV
>Mature_406_residues
MQELKVPSVGESIVEVEIGAWLKGEGESFAQDEPLVELITDKATLELPAPFAGTLKQILKRTGETARVGEAIALLEEGRA
EAAPKAQAPAEAPKEPSPEPLAMPAAERLMQEKGVSPAEVQGTGLGGRILKEDVMRHLEERAPAKPQEAPPPPPPSRPAP
PPSTPPQPPADKPWRVSEAVPMTPLRRRIAERLLMVRQTTAMLTTFNEADMSAVIALRKEFGEAFQKKYGVKLGFMSFFV
KAVVQALKEIPELNAEIRDNHIVYHRYYDIGIAVGGGEGLVVPVIRDADRLSFAEIEQKIADFAERARTKKLKPEELMGG
TFTITNGGIYGSLNSTPLLNPPQVGILGMHAIQERPVVRDGQVVIRPMMYLALSYDHRIVDGREAVTFLRRVKELIENPA
RLLLEV

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and l

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=429, Percent_Identity=28.9044289044289, Blast_Score=175, Evalue=8e-44,
Organism=Homo sapiens, GI31711992, Length=444, Percent_Identity=28.3783783783784, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI203098753, Length=450, Percent_Identity=28.6666666666667, Blast_Score=158, Evalue=8e-39,
Organism=Homo sapiens, GI203098816, Length=450, Percent_Identity=28.6666666666667, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI260898739, Length=143, Percent_Identity=35.6643356643357, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1786946, Length=414, Percent_Identity=46.1352657004831, Blast_Score=367, Evalue=1e-103,
Organism=Escherichia coli, GI1786305, Length=432, Percent_Identity=30.5555555555556, Blast_Score=175, Evalue=6e-45,
Organism=Caenorhabditis elegans, GI25146366, Length=421, Percent_Identity=39.1923990498812, Blast_Score=266, Evalue=2e-71,
Organism=Caenorhabditis elegans, GI17537937, Length=428, Percent_Identity=30.8411214953271, Blast_Score=189, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI17560088, Length=435, Percent_Identity=29.1954022988506, Blast_Score=153, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17538894, Length=307, Percent_Identity=28.6644951140065, Blast_Score=127, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6320352, Length=406, Percent_Identity=42.1182266009852, Blast_Score=299, Evalue=5e-82,
Organism=Saccharomyces cerevisiae, GI6324258, Length=447, Percent_Identity=28.4116331096197, Blast_Score=162, Evalue=9e-41,
Organism=Drosophila melanogaster, GI24645909, Length=238, Percent_Identity=52.9411764705882, Blast_Score=256, Evalue=3e-68,
Organism=Drosophila melanogaster, GI18859875, Length=435, Percent_Identity=27.3563218390805, Blast_Score=154, Evalue=8e-38,
Organism=Drosophila melanogaster, GI20129315, Length=237, Percent_Identity=29.1139240506329, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24582497, Length=237, Percent_Identity=29.1139240506329, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 44547; Mature: 44547

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQELKVPSVGESIVEVEIGAWLKGEGESFAQDEPLVELITDKATLELPAPFAGTLKQILK
CCCCCCCCCCCCEEEEEECCEECCCCCCCCCCCCHHHHHHCCCEEECCCCHHHHHHHHHH
RTGETARVGEAIALLEEGRAEAAPKAQAPAEAPKEPSPEPLAMPAAERLMQEKGVSPAEV
HCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCE
QGTGLGGRILKEDVMRHLEERAPAKPQEAPPPPPPSRPAPPPSTPPQPPADKPWRVSEAV
ECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PMTPLRRRIAERLLMVRQTTAMLTTFNEADMSAVIALRKEFGEAFQKKYGVKLGFMSFFV
CCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
KAVVQALKEIPELNAEIRDNHIVYHRYYDIGIAVGGGEGLVVPVIRDADRLSFAEIEQKI
HHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEEEECCCEEEEEEECCCCHHHHHHHHHHH
ADFAERARTKKLKPEELMGGTFTITNGGIYGSLNSTPLLNPPQVGILGMHAIQERPVVRD
HHHHHHHHHCCCCHHHHCCCEEEEECCCEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCC
GQVVIRPMMYLALSYDHRIVDGREAVTFLRRVKELIENPARLLLEV
CHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCHHHHHCCC
>Mature Secondary Structure
MQELKVPSVGESIVEVEIGAWLKGEGESFAQDEPLVELITDKATLELPAPFAGTLKQILK
CCCCCCCCCCCCEEEEEECCEECCCCCCCCCCCCHHHHHHCCCEEECCCCHHHHHHHHHH
RTGETARVGEAIALLEEGRAEAAPKAQAPAEAPKEPSPEPLAMPAAERLMQEKGVSPAEV
HCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCE
QGTGLGGRILKEDVMRHLEERAPAKPQEAPPPPPPSRPAPPPSTPPQPPADKPWRVSEAV
ECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PMTPLRRRIAERLLMVRQTTAMLTTFNEADMSAVIALRKEFGEAFQKKYGVKLGFMSFFV
CCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
KAVVQALKEIPELNAEIRDNHIVYHRYYDIGIAVGGGEGLVVPVIRDADRLSFAEIEQKI
HHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEEEECCCEEEEEEECCCCHHHHHHHHHHH
ADFAERARTKKLKPEELMGGTFTITNGGIYGSLNSTPLLNPPQVGILGMHAIQERPVVRD
HHHHHHHHHCCCCHHHHCCCEEEEECCCEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCC
GQVVIRPMMYLALSYDHRIVDGREAVTFLRRVKELIENPARLLLEV
CHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12874367 [H]