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The map label for this gene is truB

Identifier: 55980186

GI number: 55980186

Start: 212768

End: 213706

Strand: Reverse

Name: truB

Synonym: TTHA0217

Alternate gene names: 55980186

Gene position: 213706-212768 (Counterclockwise)

Preceding gene: 55980192

Following gene: 55980185

Centisome position: 11.55

GC content: 71.67

Gene sequence:

>939_bases
ATGGCCCTCTACGCGGTGGACAAGCCCCTCCACCTCACCTCCCACGACGTGGTGGAGGAGGCGAGGCGGCGCCTCTCCAC
GCGAAGGGTAGGGCACACCGGCACCTTGGACCCTTTGGCCACGGGGCTCCTCCTCCTGGTCACCAACGAGTCCACCAAGC
TCGTCCCCTTCCTCTCCGGGGAGGACAAGGAGTACATCGCCTGGGTCTCCTTCGGGGCCACCACGCCCACCCTGGACGCG
GAGGGGCCCATCAGCGAGGAGGCCCCGGCGCGCTTTGACCGGAAGGACCTCGAGGCCGCCCTGCCCCGCTTCCTGGAGGT
GCGGGAGCAGGTCCCGCCCCTCTACTCCGCCATCAAGGTGGGGGGGAAGCGGGCCTACGAGGCCGCCCGGGAGGGGAAGC
CCTTGGCGCTTGGCCCGCGCCCGGTGCGGTATTTGGAGGTGGAGCTCCTCGCCTTTGACCCCGAGCCCATCCCCCACCCC
ATCGCCCCCTCGGCCCGGGGGTGGCGGCTTGCGGAAAGGCGGGGGCGGCCCGTGCGGCTCCCGCGCCCCCTCGGGGCCTA
CCCCACGGCCGTGGTCCGGCTGGTGGTGGGGCCCGGGACCTACGTCCGCGCCTTCGCCCGGGACCTGGGGGAGATGCTTG
GGACCAAGGCCTTCCTCTCGGGGCTCGTCCGCACCCGGGTGGGCCGGGTGGGGCTGGAGCGGGCCGTGGCCCTCTCCGAC
CTCTCCCCGGAGAAGGCCATCCCGGAGCTTGACGTTTTGCCCTTTCCCGTGGTGGAGCTTTCCCACACCGAGGCCCGGCG
GGTGCTGGAGGGGATGCCCCTCCCCATCCCGGCCATGGGGTACGTGACCCTGGTGGACTCCAAGCGGCGCCTCTTGGCCA
TCGCCGAGGGGGACGGCTTCAAGCTTAAAATACGGCGCGTGTTTGCGAAGGAGGCGTAG

Upstream 100 bases:

>100_bases
GCCGGGCCTCGGCCAGGCGAAGGCGAGCCCCGGGTAGCGGGCTTCCAAAGAGGCCAGCATACCGCCATTCTATGCCCTCC
GGTGATACAATCCCCTAGGC

Downstream 100 bases:

>100_bases
CCATGGTGATCGGCGTGCCGAAGGAGATCAAGACCTTGGAGAACCGGGTGGCCCTCACGCCCGGCGGGGTGGAGAGCCTG
GTCAGGCGCGGCCACACCGT

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MALYAVDKPLHLTSHDVVEEARRRLSTRRVGHTGTLDPLATGLLLLVTNESTKLVPFLSGEDKEYIAWVSFGATTPTLDA
EGPISEEAPARFDRKDLEAALPRFLEVREQVPPLYSAIKVGGKRAYEAAREGKPLALGPRPVRYLEVELLAFDPEPIPHP
IAPSARGWRLAERRGRPVRLPRPLGAYPTAVVRLVVGPGTYVRAFARDLGEMLGTKAFLSGLVRTRVGRVGLERAVALSD
LSPEKAIPELDVLPFPVVELSHTEARRVLEGMPLPIPAMGYVTLVDSKRRLLAIAEGDGFKLKIRRVFAKEA

Sequences:

>Translated_312_residues
MALYAVDKPLHLTSHDVVEEARRRLSTRRVGHTGTLDPLATGLLLLVTNESTKLVPFLSGEDKEYIAWVSFGATTPTLDA
EGPISEEAPARFDRKDLEAALPRFLEVREQVPPLYSAIKVGGKRAYEAAREGKPLALGPRPVRYLEVELLAFDPEPIPHP
IAPSARGWRLAERRGRPVRLPRPLGAYPTAVVRLVVGPGTYVRAFARDLGEMLGTKAFLSGLVRTRVGRVGLERAVALSD
LSPEKAIPELDVLPFPVVELSHTEARRVLEGMPLPIPAMGYVTLVDSKRRLLAIAEGDGFKLKIRRVFAKEA
>Mature_311_residues
ALYAVDKPLHLTSHDVVEEARRRLSTRRVGHTGTLDPLATGLLLLVTNESTKLVPFLSGEDKEYIAWVSFGATTPTLDAE
GPISEEAPARFDRKDLEAALPRFLEVREQVPPLYSAIKVGGKRAYEAAREGKPLALGPRPVRYLEVELLAFDPEPIPHPI
APSARGWRLAERRGRPVRLPRPLGAYPTAVVRLVVGPGTYVRAFARDLGEMLGTKAFLSGLVRTRVGRVGLERAVALSDL
SPEKAIPELDVLPFPVVELSHTEARRVLEGMPLPIPAMGYVTLVDSKRRLLAIAEGDGFKLKIRRVFAKEA

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=233, Percent_Identity=29.1845493562232, Blast_Score=90, Evalue=2e-18,
Organism=Escherichia coli, GI2367200, Length=287, Percent_Identity=31.3588850174216, Blast_Score=124, Evalue=8e-30,
Organism=Saccharomyces cerevisiae, GI6324037, Length=257, Percent_Identity=28.7937743190661, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6323204, Length=226, Percent_Identity=26.9911504424779, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_THET2 (Q72GS9)

Other databases:

- EMBL:   AE017221
- RefSeq:   YP_005738.1
- ProteinModelPortal:   Q72GS9
- SMR:   Q72GS9
- STRING:   Q72GS9
- GeneID:   2775813
- GenomeReviews:   AE017221_GR
- eggNOG:   COG0130
- HOGENOM:   HBG397258
- OMA:   EYLGTIR
- PhylomeDB:   Q72GS9
- ProtClustDB:   PRK14122
- BioCyc:   TTHE262724:TT_C1769-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015225
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF09142 TruB_C; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 34292; Mature: 34161

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: NA

Important sites: ACT_SITE 37-37

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALYAVDKPLHLTSHDVVEEARRRLSTRRVGHTGTLDPLATGLLLLVTNESTKLVPFLSG
CEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHCCEEEEEECCCCEEEEEECC
EDKEYIAWVSFGATTPTLDAEGPISEEAPARFDRKDLEAALPRFLEVREQVPPLYSAIKV
CCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHH
GGKRAYEAAREGKPLALGPRPVRYLEVELLAFDPEPIPHPIAPSARGWRLAERRGRPVRL
CCHHHHHHHHCCCCEEECCCCCEEEEEEEEEECCCCCCCCCCCCCCCCHHHHHCCCCEEC
PRPLGAYPTAVVRLVVGPGTYVRAFARDLGEMLGTKAFLSGLVRTRVGRVGLERAVALSD
CCCCCCCHHEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LSPEKAIPELDVLPFPVVELSHTEARRVLEGMPLPIPAMGYVTLVDSKRRLLAIAEGDGF
CCHHHCCCCCCCCCCCCEECCHHHHHHHHCCCCCCCCCCCEEEEEECCCEEEEEECCCCC
KLKIRRVFAKEA
EEEEEEHHHCCC
>Mature Secondary Structure 
ALYAVDKPLHLTSHDVVEEARRRLSTRRVGHTGTLDPLATGLLLLVTNESTKLVPFLSG
EEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHCCEEEEEECCCCEEEEEECC
EDKEYIAWVSFGATTPTLDAEGPISEEAPARFDRKDLEAALPRFLEVREQVPPLYSAIKV
CCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHH
GGKRAYEAAREGKPLALGPRPVRYLEVELLAFDPEPIPHPIAPSARGWRLAERRGRPVRL
CCHHHHHHHHCCCCEEECCCCCEEEEEEEEEECCCCCCCCCCCCCCCCHHHHHCCCCEEC
PRPLGAYPTAVVRLVVGPGTYVRAFARDLGEMLGTKAFLSGLVRTRVGRVGLERAVALSD
CCCCCCCHHEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LSPEKAIPELDVLPFPVVELSHTEARRVLEGMPLPIPAMGYVTLVDSKRRLLAIAEGDGF
CCHHHCCCCCCCCCCCCEECCHHHHHHHHCCCCCCCCCCCEEEEEECCCEEEEEECCCCC
KLKIRRVFAKEA
EEEEEEHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA