Click here to switch to the map view.

The map label for this gene is hisZ

Identifier: 55980098

GI number: 55980098

Start: 126976

End: 128061

Strand: Reverse

Name: hisZ

Synonym: TTHA0129

Alternate gene names: 55980098

Gene position: 128061-126976 (Counterclockwise)

Preceding gene: 55980099

Following gene: 55980097

Centisome position: 6.92

GC content: 70.9

Gene sequence:

>1086_bases
ATGATCCCCGAGGGCACCCGCTTCCTTTTGCCCCCCGAGGCCCGGCTCAAGGCCGAGGTGGTGGGGAAGCTCCAGCACCT
CTTCCGGCGCCACGGCTACGAGCCCGTGGAGCTTCCCGCCCTGGAGCTTTACGACCCCGACCACCCCCTGGCGGAAAGGG
CCTTCAAGCTGGTGGACAAGACGGGGGAGGTCTTGGCCTTAAGGAGCGAGTTCACCACCCTCCTCGCCAAGCTCCTCCGG
GCCCACCTGGGAGAGGGCGCGCACCGCTTCCAGTACGCTGGGCCCCTTTGGCTCAGGGAGGCGGACGCGGAGCTTGGGCG
CCTTCGGGAGTACACCCAGGTGGGCCTGGAGCTTTTGGGGGCCACGGGGCCCTTGGCGGACGCCGAGGTGCTGGAGCTGG
CCTTCGCCGCCCTCGAGGCCTTAGGGGTCCAGGGGGAGGTGGAGGTGGGCTTGCCGAGCCTCGTGGGGGAGGTGCTCAAG
GCCTCGGGGCTTCCCGAGGCCCTGCAAAGGCGGGCCCAGCAGGCCATCCACCGCAAAAACCTCCCCGAGCTCAAGGGGCT
TCTCGCAGAAAGCCCCGTCCCCGAGGAGGCGCGGAAGGTCCTCCTCGCCCTCCCCGACCTCTACGGAGGGCGGGAGGTCT
TGAAGGAGGCGCGGGGCCTTCCGCTGCCCCCCAAGGCCCAGGAGGCCCTCGCCCAGCTGGAGAGGACCCTGGACCTCCTG
GGAAGGCCCGTCCTCCTGGACCTGGGCATGGCCCGGCGCTACGAGTACTACTCGGGGATCTTCTTCCGGGCCTACACCCC
GGGGTTCGGCCTTCCCCTCCTCGGGGGCGGGCGGTACGACGGGGCCCTTTTCCCCAAGGCCGCGGGCTTCGCCCTGGGGG
TGGAGAGGCTCCTCGAGGCCTTGCGGCTTCCCAAGGAGGAGGAGCCCCCGGAGGTCTTGGCCCTGGACCTCAAGGCCCTC
CGCCGCTTCGCCCGGGAGCGGCGCACGGAGCTTTTCCACGGGGAGGACCCCGTGGCCTACGCCCGAAGGCGCGGCATCCC
CTTCCTCGCCCGGGGGGAGGAGCTTTTCCGCGTGGAGGAGGCATGA

Upstream 100 bases:

>100_bases
GCTCCTCTTCCTGGAGGGCCCCCGCCCCGAGGTCACCTACCGGGACCGCAAAGGGAAGTACCAGGGGCAGAAGGGGATCA
CCCTCCCCCGGGTTTAGGCC

Downstream 100 bases:

>100_bases
GGCGCTTCGCCCTCACCGTGGCCCTGCCCAAGGGGCGGATGTTCCGGGAGGCCTACGAGGTCCTCAAGCGGGCGGGCTTG
GACCTCCCCGAGGTGGAGGG

Product: ATP phosphoribosyltransferase regulatory subunit

Products: AMP; diphosphate +L-histidyl-tRNAHis [C]

Alternate protein names: NA

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MIPEGTRFLLPPEARLKAEVVGKLQHLFRRHGYEPVELPALELYDPDHPLAERAFKLVDKTGEVLALRSEFTTLLAKLLR
AHLGEGAHRFQYAGPLWLREADAELGRLREYTQVGLELLGATGPLADAEVLELAFAALEALGVQGEVEVGLPSLVGEVLK
ASGLPEALQRRAQQAIHRKNLPELKGLLAESPVPEEARKVLLALPDLYGGREVLKEARGLPLPPKAQEALAQLERTLDLL
GRPVLLDLGMARRYEYYSGIFFRAYTPGFGLPLLGGGRYDGALFPKAAGFALGVERLLEALRLPKEEEPPEVLALDLKAL
RRFARERRTELFHGEDPVAYARRRGIPFLARGEELFRVEEA

Sequences:

>Translated_361_residues
MIPEGTRFLLPPEARLKAEVVGKLQHLFRRHGYEPVELPALELYDPDHPLAERAFKLVDKTGEVLALRSEFTTLLAKLLR
AHLGEGAHRFQYAGPLWLREADAELGRLREYTQVGLELLGATGPLADAEVLELAFAALEALGVQGEVEVGLPSLVGEVLK
ASGLPEALQRRAQQAIHRKNLPELKGLLAESPVPEEARKVLLALPDLYGGREVLKEARGLPLPPKAQEALAQLERTLDLL
GRPVLLDLGMARRYEYYSGIFFRAYTPGFGLPLLGGGRYDGALFPKAAGFALGVERLLEALRLPKEEEPPEVLALDLKAL
RRFARERRTELFHGEDPVAYARRRGIPFLARGEELFRVEEA
>Mature_361_residues
MIPEGTRFLLPPEARLKAEVVGKLQHLFRRHGYEPVELPALELYDPDHPLAERAFKLVDKTGEVLALRSEFTTLLAKLLR
AHLGEGAHRFQYAGPLWLREADAELGRLREYTQVGLELLGATGPLADAEVLELAFAALEALGVQGEVEVGLPSLVGEVLK
ASGLPEALQRRAQQAIHRKNLPELKGLLAESPVPEEARKVLLALPDLYGGREVLKEARGLPLPPKAQEALAQLERTLDLL
GRPVLLDLGMARRYEYYSGIFFRAYTPGFGLPLLGGGRYDGALFPKAAGFALGVERLLEALRLPKEEEPPEVLALDLKAL
RRFARERRTELFHGEDPVAYARRRGIPFLARGEELFRVEEA

Specific function: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine

COG id: COG3705

COG function: function code E; ATP phosphoribosyltransferase involved in histidine biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily

Homologues:

Organism=Homo sapiens, GI15029520, Length=413, Percent_Identity=24.6973365617433, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI6996014, Length=412, Percent_Identity=25.2427184466019, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1788861, Length=329, Percent_Identity=27.6595744680851, Blast_Score=82, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24643061, Length=353, Percent_Identity=26.0623229461756, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24643059, Length=372, Percent_Identity=24.7311827956989, Blast_Score=86, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HISZ_THET2 (P62360)

Other databases:

- EMBL:   AE017221
- RefSeq:   YP_005834.1
- ProteinModelPortal:   P62360
- SMR:   P62360
- STRING:   P62360
- GeneID:   2775515
- GenomeReviews:   AE017221_GR
- eggNOG:   COG3705
- HOGENOM:   HBG616575
- OMA:   DLRGYHY
- ProtClustDB:   PRK12292
- BioCyc:   TTHE262724:TT_C1865-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00125
- InterPro:   IPR006195
- InterPro:   IPR004517
- InterPro:   IPR004516
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00443

Pfam domain/function: NA

EC number: 6.1.1.21 [C]

Molecular weight: Translated: 40054; Mature: 40054

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPEGTRFLLPPEARLKAEVVGKLQHLFRRHGYEPVELPALELYDPDHPLAERAFKLVDK
CCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHC
TGEVLALRSEFTTLLAKLLRAHLGEGAHRFQYAGPLWLREADAELGRLREYTQVGLELLG
CCCEEEHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
ATGPLADAEVLELAFAALEALGVQGEVEVGLPSLVGEVLKASGLPEALQRRAQQAIHRKN
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
LPELKGLLAESPVPEEARKVLLALPDLYGGREVLKEARGLPLPPKAQEALAQLERTLDLL
CHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
GRPVLLDLGMARRYEYYSGIFFRAYTPGFGLPLLGGGRYDGALFPKAAGFALGVERLLEA
CCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LRLPKEEEPPEVLALDLKALRRFARERRTELFHGEDPVAYARRRGIPFLARGEELFRVEE
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEECCHHHHHCCC
A
C
>Mature Secondary Structure
MIPEGTRFLLPPEARLKAEVVGKLQHLFRRHGYEPVELPALELYDPDHPLAERAFKLVDK
CCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHC
TGEVLALRSEFTTLLAKLLRAHLGEGAHRFQYAGPLWLREADAELGRLREYTQVGLELLG
CCCEEEHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
ATGPLADAEVLELAFAALEALGVQGEVEVGLPSLVGEVLKASGLPEALQRRAQQAIHRKN
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
LPELKGLLAESPVPEEARKVLLALPDLYGGREVLKEARGLPLPPKAQEALAQLERTLDLL
CHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
GRPVLLDLGMARRYEYYSGIFFRAYTPGFGLPLLGGGRYDGALFPKAAGFALGVERLLEA
CCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LRLPKEEEPPEVLALDLKALRRFARERRTELFHGEDPVAYARRRGIPFLARGEELFRVEE
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEECCHHHHHCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): 8520 [C]

Specific activity: 121.8

Km value (mM): 0.06 {His}} 0.046 {His}} 0.03 {His}} 0.02 {His}} 0.017 {His}} 0.011 {His}} 0.008 {His}} 0.0014 {tRNAHis}} 0.00056 {tRNAHis}} 0.0016 {U73tRNAHisGUG}} 0.00031 {U73tRNAHisGUG}} 0.89 {ATP}} 0.74 {ATP}} 0.655 {ATP}} 0.5

Substrates: ATP; @HIST01.txt*L-histidine!; tRNAHis [C]

Specific reaction: ATP + @HIST01.txt*L-histidine! + tRNAHis = AMP + diphosphate +L-histidyl-tRNAHis [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 2, 4-Triazole-3-alanine; D-Histidine; Diphosphate; L-Histidinol; Mg2+; N-Acetyl histidine; NEM; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA