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The map label for this gene is nqo14

Identifier: 55980066

GI number: 55980066

Start: 96408

End: 97691

Strand: Direct

Name: nqo14

Synonym: TTHA0097

Alternate gene names: 55980066

Gene position: 96408-97691 (Clockwise)

Preceding gene: 55980065

Following gene: 55980067

Centisome position: 5.21

GC content: 72.51

Gene sequence:

>1284_bases
ATGACCCTGGCGATCCTCGCGGTCTTCTCCGTGGCCTTGACCCTTCTCGGTTTCGTCCTCCCGCCCCAGGGCGTGAAGCG
GGCCACGCTCCTGGGCCTGGCCCTGGCCCTCGCAAGCCTCCTCCTCACCTGGGGGAAGCCCTTCGCCTTCGGGCCCTACG
CGGTGGACGGCGTCTCCCAGGTCTTCACCCTCCTCGCCCTCCTGGGGGCCTTGTGGACCGTGGGCCTCGTGCGCTCGGGG
CGGTTTGAGTTCTACCTCCTCGTCCTCTACGCCGCCTTGGGCATGCACCTCCTCGCCTCCACCCGGCACCTCCTCCTGAT
GCTCGTGGCCCTCGAGGCCCTCTCCCTCCCCCTCTACGCCCTCGCCACCTGGCGCCGGGGGCAGGGCCTCGAGGCCGCCC
TCAAGTACTTCCTCCTGGGGGCCTTGGCCGCCGCCTTCTTCCTCTACGGGGCGGCCCTCTTCTACGGGGCCACGGGGAGC
CTCGTCCTGGGCGCCCCCGGGGAAGGCCCCCTCTACGCCCTGGCCCTGGGCCTGCTCCTCGTGGGCCTGGGCTTTAAGGC
GGCCCTCGCCCCCTTCCACTTCTGGACCCCCGACGTCTACCAGGGAAGCCCCACCCCCGTGGTCCTCTTCATGGCCACCT
CGGTGAAGGCCGCGGCCTTCGCCGCCCTCCTCCGGGTGGCCGCACCCCCCGAGGCCCTCGCCCTCCTCGTGGCCCTCTCC
GTGGTGGTGGGGAACCTGGCGGCCCTGGCGCAGAAGGAGGCCAAGCGGCTTCTCGCCTACTCCTCCATCGCCCACGCCGG
GTACATGGCCCTCGCCCTCTACACCGGAAACGCCCAGGCCCTGGGCTTCTACCTCCTCACCTACGTCCTGGCCACGGGCC
TCGCCTTCGCCGTCCTAAGCCAGATCTCCCCGGACCGGGTGCCCCTGGAAGCCCTCCGGGGCCTCTACCGGAAAGACCCC
CTCCTGGGCCTCGCCTTCCTGGTGGCCATGCTTTCCCTGCTGGGCCTGCCGCCCCTCGCGGGCTTTTGGGGCAAGTACCT
GGCCTTCGCCGAGGCGGCCCGGGCCGGGGCCTGGGGGGTCTTGGTCCTGGCCCTCGTCACCAGCGCCGTGAGCGCCTACT
ACTACCTCGGGCTCGGGCTTGCCGTCTTCGCGCGGCCCGAGGAGACCCCCTTCCGCCCAGGCCCTCCCTGGGCCCGGGCC
GCGGTCGTGGCCGCCGGGGTCCTCCTCCTCGCCTTGGGGCTCCTCCCCGGCCTCGTCCTCCCCGCCTTGGCCGCGGGGGG
TTAA

Upstream 100 bases:

>100_bases
GCGTCCTCGCCCTCCTCCTCATGGGGGTCTTCCCCGGCTACTTCGCCCGGGGGCTCCACCCTCTGGCGGAGGCCTTCGCC
AAGCTTCTTGGAGGTGGCGC

Downstream 100 bases:

>100_bases
GATAACCCCCTGATGCTACGGCGCGCGCTGGAAGAGGCCATCGCCCAGGCCCTAAAGGAGATGGGGGTTCCGGTCCGCCT
CAAGGTGGCCCGGGCCCCCA

Product: NADH-quinone oxidoreductase subunit 14

Products: NA

Alternate protein names: NADH dehydrogenase I chain 14; NDH-1 subunit 14

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MTLAILAVFSVALTLLGFVLPPQGVKRATLLGLALALASLLLTWGKPFAFGPYAVDGVSQVFTLLALLGALWTVGLVRSG
RFEFYLLVLYAALGMHLLASTRHLLLMLVALEALSLPLYALATWRRGQGLEAALKYFLLGALAAAFFLYGAALFYGATGS
LVLGAPGEGPLYALALGLLLVGLGFKAALAPFHFWTPDVYQGSPTPVVLFMATSVKAAAFAALLRVAAPPEALALLVALS
VVVGNLAALAQKEAKRLLAYSSIAHAGYMALALYTGNAQALGFYLLTYVLATGLAFAVLSQISPDRVPLEALRGLYRKDP
LLGLAFLVAMLSLLGLPPLAGFWGKYLAFAEAARAGAWGVLVLALVTSAVSAYYYLGLGLAVFARPEETPFRPGPPWARA
AVVAAGVLLLALGLLPGLVLPALAAGG

Sequences:

>Translated_427_residues
MTLAILAVFSVALTLLGFVLPPQGVKRATLLGLALALASLLLTWGKPFAFGPYAVDGVSQVFTLLALLGALWTVGLVRSG
RFEFYLLVLYAALGMHLLASTRHLLLMLVALEALSLPLYALATWRRGQGLEAALKYFLLGALAAAFFLYGAALFYGATGS
LVLGAPGEGPLYALALGLLLVGLGFKAALAPFHFWTPDVYQGSPTPVVLFMATSVKAAAFAALLRVAAPPEALALLVALS
VVVGNLAALAQKEAKRLLAYSSIAHAGYMALALYTGNAQALGFYLLTYVLATGLAFAVLSQISPDRVPLEALRGLYRKDP
LLGLAFLVAMLSLLGLPPLAGFWGKYLAFAEAARAGAWGVLVLALVTSAVSAYYYLGLGLAVFARPEETPFRPGPPWARA
AVVAAGVLLLALGLLPGLVLPALAAGG
>Mature_426_residues
TLAILAVFSVALTLLGFVLPPQGVKRATLLGLALALASLLLTWGKPFAFGPYAVDGVSQVFTLLALLGALWTVGLVRSGR
FEFYLLVLYAALGMHLLASTRHLLLMLVALEALSLPLYALATWRRGQGLEAALKYFLLGALAAAFFLYGAALFYGATGSL
VLGAPGEGPLYALALGLLLVGLGFKAALAPFHFWTPDVYQGSPTPVVLFMATSVKAAAFAALLRVAAPPEALALLVALSV
VVGNLAALAQKEAKRLLAYSSIAHAGYMALALYTGNAQALGFYLLTYVLATGLAFAVLSQISPDRVPLEALRGLYRKDPL
LGLAFLVAMLSLLGLPPLAGFWGKYLAFAEAARAGAWGVLVLALVTSAVSAYYYLGLGLAVFARPEETPFRPGPPWARAA
VVAAGVLLLALGLLPGLVLPALAAGG

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family

Homologues:

Organism=Homo sapiens, GI251831108, Length=219, Percent_Identity=30.1369863013699, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI145693160, Length=403, Percent_Identity=39.2059553349876, Blast_Score=225, Evalue=4e-60,
Organism=Escherichia coli, GI1788831, Length=354, Percent_Identity=26.271186440678, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1788829, Length=337, Percent_Identity=28.486646884273, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1788613, Length=336, Percent_Identity=28.2738095238095, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788614, Length=332, Percent_Identity=28.6144578313253, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NQO14_THET8 (Q56229)

Other databases:

- EMBL:   U52917
- EMBL:   AP008226
- PIR:   T11911
- RefSeq:   YP_143363.1
- ProteinModelPortal:   Q56229
- STRING:   Q56229
- GeneID:   3169623
- GenomeReviews:   AP008226_GR
- KEGG:   ttj:TTHA0097
- NMPDR:   fig|300852.3.peg.128
- eggNOG:   COG1007
- HOGENOM:   HBG747830
- OMA:   YYLRFLK
- ProtClustDB:   CLSK738965
- BioCyc:   TTHE300852:TTHA0097-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_00445
- InterPro:   IPR010096
- InterPro:   IPR001750
- TIGRFAMs:   TIGR01770

Pfam domain/function: PF00361 Oxidored_q1

EC number: =1.6.99.5

Molecular weight: Translated: 44437; Mature: 44306

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x12e09e9c)-; HASH(0x11156aec)-; HASH(0x11f024fc)-; HASH(0x11bb4d14)-; HASH(0xd45e354)-; HASH(0xe3d50d0)-; HASH(0x11d303b0)-; HASH(0x11eff1e0)-; HASH(0x11cccb4c)-; HASH(0x11efc770)-; HASH(0x11986814)-; HASH(0x11ccc8e8)-; HASH(0x11efc68c)-; HASH(0x11e7ec48)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLAILAVFSVALTLLGFVLPPQGVKRATLLGLALALASLLLTWGKPFAFGPYAVDGVSQ
CHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
VFTLLALLGALWTVGLVRSGRFEFYLLVLYAALGMHLLASTRHLLLMLVALEALSLPLYA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
LATWRRGQGLEAALKYFLLGALAAAFFLYGAALFYGATGSLVLGAPGEGPLYALALGLLL
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHH
VGLGFKAALAPFHFWTPDVYQGSPTPVVLFMATSVKAAAFAALLRVAAPPEALALLVALS
HHCCHHHHHCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHH
VVVGNLAALAQKEAKRLLAYSSIAHAGYMALALYTGNAQALGFYLLTYVLATGLAFAVLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
QISPDRVPLEALRGLYRKDPLLGLAFLVAMLSLLGLPPLAGFWGKYLAFAEAARAGAWGV
HCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHH
LVLALVTSAVSAYYYLGLGLAVFARPEETPFRPGPPWARAAVVAAGVLLLALGLLPGLVL
HHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
PALAAGG
HHHHCCH
>Mature Secondary Structure 
TLAILAVFSVALTLLGFVLPPQGVKRATLLGLALALASLLLTWGKPFAFGPYAVDGVSQ
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
VFTLLALLGALWTVGLVRSGRFEFYLLVLYAALGMHLLASTRHLLLMLVALEALSLPLYA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
LATWRRGQGLEAALKYFLLGALAAAFFLYGAALFYGATGSLVLGAPGEGPLYALALGLLL
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHH
VGLGFKAALAPFHFWTPDVYQGSPTPVVLFMATSVKAAAFAALLRVAAPPEALALLVALS
HHCCHHHHHCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHH
VVVGNLAALAQKEAKRLLAYSSIAHAGYMALALYTGNAQALGFYLLTYVLATGLAFAVLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
QISPDRVPLEALRGLYRKDPLLGLAFLVAMLSLLGLPPLAGFWGKYLAFAEAARAGAWGV
HCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHH
LVLALVTSAVSAYYYLGLGLAVFARPEETPFRPGPPWARAAVVAAGVLLLALGLLPGLVL
HHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
PALAAGG
HHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9020134