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The map label for this gene is nqo12

Identifier: 55980064

GI number: 55980064

Start: 93188

End: 95008

Strand: Direct

Name: nqo12

Synonym: TTHA0095

Alternate gene names: 55980064

Gene position: 93188-95008 (Clockwise)

Preceding gene: 55980063

Following gene: 55980065

Centisome position: 5.04

GC content: 68.04

Gene sequence:

>1821_bases
ATGGCGCTTCTCGGGACGATTCTCCTGCCCTTGTTGGGCTTCGCCCTCCTCGGCCTCTTCGGCAAAAGGATGCGAGAGCC
CCTCCCCGGGGTCCTGGCCTCGGGGCTCGTCCTCGCCTCCTTCCTCCTGGGGGCGGGGCTTCTCCTCTCGGGCGGGGCCC
GCTTCCAAGCGGAGTGGCTTCCCGGCATCCCCTTCAGCCTCCTTCTGGACAACCTCTCCGGCTTCATGCTCCTCATCGTC
ACCGGCGTGGGCTTCCTCATCCACGTCTACGCCATCGGCTACATGGGGGGGGACCCGGGCTACAGCCGCTTCTTCGCCTA
CTTCAACCTCTTCATCGCCATGATGCTCACCCTGGTCCTCGCCGACAGCTACCCGGTGATGTTCATCGGCTGGGAGGGGG
TGGGCCTGGCGAGCTTCCTCCTCATCGGCTTCTGGTACAAGAACCCCCAGTACGCCGACAGCGCCCGCAAGGCCTTCATC
GTGAACCGGATCGGCGACCTGGGCTTCATGCTGGGCATGGCCATCCTCTGGGCCCTCTACGGCACCCTCTCCATCAGCGA
GCTCAAGGAGGCGATGGAGGGCCCCCTGAAGAACCCTGACCTCCTCGCCCTGGCAGGGCTTCTCCTCTTCCTCGGGGCTG
TGGGCAAGAGCGCCCAGATCCCCCTCATGGTCTGGCTCCCCGACGCCATGGCCGGCCCCACCCCCGTCTCCGCCCTGATC
CACGCGGCCACCATGGTGACGGCCGGGGTCTACCTCATCGCCCGGAGCTCCTTCCTCTACAGCGTCCTCCCCGACGTCTC
CTACGCCATCGCCGTGGTCGGCCTCCTCACCGCGGCTTATGGGGCGCTATCCGCCTTCGGCCAGACCGATATCAAGAAAA
TCGTCGCCTACTCCACCATCAGCCAGCTCGGGTACATGTTCCTGGCGGCCGGGGTGGGGGCGTACTGGGTGGCCCTCTTC
CACGTCTTCACCCACGCCTTCTTCAAGGCCCTCCTCTTCCTGGCCTCGGGGAGCGTGATCCACGCCCTGGGGGGCGAGCA
GGACGTGCGCAAGATGGGGGGCCTCTGGAAGCACCTGCCCCAAACCCGCTGGCACGCCCTCATCGGGGCCCTGGCCCTGG
GCGGCCTCCCCTTGCTTTCCGGCTTCTGGTCCAAGGACGCCATCCTCGCCGCCACCCTCACCTACCCCTTCGGGGGAGTG
GGGTTCTACGTGGGGGCGCTCCTCGTGGCGGTCCTCACCGCCATGTACGCCATGCGCTGGTTCGTCCTGGTCTTCCTGGG
GGAGGAGAGGGGGCACCACCACCCCCACGAGGCCCCGCCGGTGATGCTCTGGCCCAACCACCTCCTGGCCCTGGGCTCGG
TCCTCGCGGGGTACCTGGCCCTGCCCCACCCCTTGCCCAACGTCCTGGAACCCTTCCTGAAGCCCGCCCTGGCGGAGGTG
GAGGCCCACCACCTCTCCCTGGGGGCGGAGTGGGGCCTCATCGCCCTCTCGGCGGCGGTGGCCCTTCTAGGGCTCTGGGC
GGGTTTCGTCTTCTTCCAGCGCAAGGTTTTCCCCGCCTGGTACCTGGCCTTTGAGGCGGCGAGCCGGGAGGCCTTCTACG
TGGACCGGGCCTACAACGCCCTCATCGTGAACCCCTTGAAGGCCCTGGCGGAGGCCCTCTTCTACGGGGATCGGGGCCTC
CTAAGCGGCTACTTCGGCCTGGGCGGGGCGGCGCGGAGCCTGGGGCAGGGCCTCGCCCGGTTGCAGACCGGCTACCTTAG
GGTTTACGCCCTCCTCTTCGTGCTGGGGGCGCTTCTCCTTCTGGGGGTGATGCGGTGGTAG

Upstream 100 bases:

>100_bases
CCGCCGCCGAGGTGGCCGTGGGCCTCGGCCTCATCGTGGCCATCTTCCGCCACCGGGAGAGCACCGCGGTGGACGACCTC
TCGGAGCTTCGGGGGTAAGC

Downstream 100 bases:

>100_bases
TCCTGGCGGTCCTTCTACCGGTGGTCTTCGGGGCCCTGCTCCTTTTGGGCCTGCCCCGGGCCCTCGGGGTTTTGGGGGCG
GGGCTTTCCTTCCTCCTCAA

Product: NADH-quinone oxidoreductase subunit 12

Products: NA

Alternate protein names: NADH dehydrogenase I chain 12; NDH-1 subunit 12

Number of amino acids: Translated: 606; Mature: 605

Protein sequence:

>606_residues
MALLGTILLPLLGFALLGLFGKRMREPLPGVLASGLVLASFLLGAGLLLSGGARFQAEWLPGIPFSLLLDNLSGFMLLIV
TGVGFLIHVYAIGYMGGDPGYSRFFAYFNLFIAMMLTLVLADSYPVMFIGWEGVGLASFLLIGFWYKNPQYADSARKAFI
VNRIGDLGFMLGMAILWALYGTLSISELKEAMEGPLKNPDLLALAGLLLFLGAVGKSAQIPLMVWLPDAMAGPTPVSALI
HAATMVTAGVYLIARSSFLYSVLPDVSYAIAVVGLLTAAYGALSAFGQTDIKKIVAYSTISQLGYMFLAAGVGAYWVALF
HVFTHAFFKALLFLASGSVIHALGGEQDVRKMGGLWKHLPQTRWHALIGALALGGLPLLSGFWSKDAILAATLTYPFGGV
GFYVGALLVAVLTAMYAMRWFVLVFLGEERGHHHPHEAPPVMLWPNHLLALGSVLAGYLALPHPLPNVLEPFLKPALAEV
EAHHLSLGAEWGLIALSAAVALLGLWAGFVFFQRKVFPAWYLAFEAASREAFYVDRAYNALIVNPLKALAEALFYGDRGL
LSGYFGLGGAARSLGQGLARLQTGYLRVYALLFVLGALLLLGVMRW

Sequences:

>Translated_606_residues
MALLGTILLPLLGFALLGLFGKRMREPLPGVLASGLVLASFLLGAGLLLSGGARFQAEWLPGIPFSLLLDNLSGFMLLIV
TGVGFLIHVYAIGYMGGDPGYSRFFAYFNLFIAMMLTLVLADSYPVMFIGWEGVGLASFLLIGFWYKNPQYADSARKAFI
VNRIGDLGFMLGMAILWALYGTLSISELKEAMEGPLKNPDLLALAGLLLFLGAVGKSAQIPLMVWLPDAMAGPTPVSALI
HAATMVTAGVYLIARSSFLYSVLPDVSYAIAVVGLLTAAYGALSAFGQTDIKKIVAYSTISQLGYMFLAAGVGAYWVALF
HVFTHAFFKALLFLASGSVIHALGGEQDVRKMGGLWKHLPQTRWHALIGALALGGLPLLSGFWSKDAILAATLTYPFGGV
GFYVGALLVAVLTAMYAMRWFVLVFLGEERGHHHPHEAPPVMLWPNHLLALGSVLAGYLALPHPLPNVLEPFLKPALAEV
EAHHLSLGAEWGLIALSAAVALLGLWAGFVFFQRKVFPAWYLAFEAASREAFYVDRAYNALIVNPLKALAEALFYGDRGL
LSGYFGLGGAARSLGQGLARLQTGYLRVYALLFVLGALLLLGVMRW
>Mature_605_residues
ALLGTILLPLLGFALLGLFGKRMREPLPGVLASGLVLASFLLGAGLLLSGGARFQAEWLPGIPFSLLLDNLSGFMLLIVT
GVGFLIHVYAIGYMGGDPGYSRFFAYFNLFIAMMLTLVLADSYPVMFIGWEGVGLASFLLIGFWYKNPQYADSARKAFIV
NRIGDLGFMLGMAILWALYGTLSISELKEAMEGPLKNPDLLALAGLLLFLGAVGKSAQIPLMVWLPDAMAGPTPVSALIH
AATMVTAGVYLIARSSFLYSVLPDVSYAIAVVGLLTAAYGALSAFGQTDIKKIVAYSTISQLGYMFLAAGVGAYWVALFH
VFTHAFFKALLFLASGSVIHALGGEQDVRKMGGLWKHLPQTRWHALIGALALGGLPLLSGFWSKDAILAATLTYPFGGVG
FYVGALLVAVLTAMYAMRWFVLVFLGEERGHHHPHEAPPVMLWPNHLLALGSVLAGYLALPHPLPNVLEPFLKPALAEVE
AHHLSLGAEWGLIALSAAVALLGLWAGFVFFQRKVFPAWYLAFEAASREAFYVDRAYNALIVNPLKALAEALFYGDRGLL
SGYFGLGGAARSLGQGLARLQTGYLRVYALLFVLGALLLLGVMRW

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family

Homologues:

Organism=Homo sapiens, GI251831117, Length=396, Percent_Identity=42.6767676767677, Blast_Score=271, Evalue=1e-72,
Organism=Escherichia coli, GI1788614, Length=606, Percent_Identity=43.0693069306931, Blast_Score=379, Evalue=1e-106,
Organism=Escherichia coli, GI1788829, Length=340, Percent_Identity=34.1176470588235, Blast_Score=163, Evalue=3e-41,
Organism=Escherichia coli, GI1788831, Length=347, Percent_Identity=29.3948126801153, Blast_Score=117, Evalue=3e-27,
Organism=Escherichia coli, GI1788827, Length=447, Percent_Identity=29.082774049217, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI2367154, Length=405, Percent_Identity=27.6543209876543, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI145693160, Length=194, Percent_Identity=30.4123711340206, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788613, Length=376, Percent_Identity=26.063829787234, Blast_Score=78, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NQO12_THET8 (Q56227)

Other databases:

- EMBL:   U52917
- EMBL:   AP008226
- PIR:   T11909
- RefSeq:   YP_143361.1
- ProteinModelPortal:   Q56227
- STRING:   Q56227
- GeneID:   3169613
- GenomeReviews:   AP008226_GR
- KEGG:   ttj:TTHA0095
- NMPDR:   fig|300852.3.peg.126
- eggNOG:   COG1009
- HOGENOM:   HBG643593
- OMA:   IRSYAGW
- ProtClustDB:   CLSK2762015
- BioCyc:   TTHE300852:TTHA0095-MONOMER
- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR003945
- InterPro:   IPR018393
- PRINTS:   PR01435
- TIGRFAMs:   TIGR01974

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N

EC number: =1.6.99.5

Molecular weight: Translated: 65142; Mature: 65011

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x122f4644)-; HASH(0x11d02844)-; HASH(0x126ec948)-; HASH(0x126d7094)-; HASH(0x11a76d18)-; HASH(0x12718554)-; HASH(0x12a4a284)-; HASH(0x102b2824)-; HASH(0x126d530c)-; HASH(0x11f2f5b8)-; HASH(0x11f34574)-; HASH(0x11f2f4b0)-; HASH(0x126e86e0)-; HASH(0x126ea62c)-; HASH(0x11f34604)-; HASH(0x126e0444)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALLGTILLPLLGFALLGLFGKRMREPLPGVLASGLVLASFLLGAGLLLSGGARFQAEWL
CHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCC
PGIPFSLLLDNLSGFMLLIVTGVGFLIHVYAIGYMGGDPGYSRFFAYFNLFIAMMLTLVL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
ADSYPVMFIGWEGVGLASFLLIGFWYKNPQYADSARKAFIVNRIGDLGFMLGMAILWALY
HCCCCEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GTLSISELKEAMEGPLKNPDLLALAGLLLFLGAVGKSAQIPLMVWLPDAMAGPTPVSALI
HCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCHHHHHH
HAATMVTAGVYLIARSSFLYSVLPDVSYAIAVVGLLTAAYGALSAFGQTDIKKIVAYSTI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
SQLGYMFLAAGVGAYWVALFHVFTHAFFKALLFLASGSVIHALGGEQDVRKMGGLWKHLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHCC
QTRWHALIGALALGGLPLLSGFWSKDAILAATLTYPFGGVGFYVGALLVAVLTAMYAMRW
HHHHHHHHHHHHHCCHHHHHCCCCCCHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
FVLVFLGEERGHHHPHEAPPVMLWPNHLLALGSVLAGYLALPHPLPNVLEPFLKPALAEV
HHHHHHCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
EAHHLSLGAEWGLIALSAAVALLGLWAGFVFFQRKVFPAWYLAFEAASREAFYVDRAYNA
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHCCH
LIVNPLKALAEALFYGDRGLLSGYFGLGGAARSLGQGLARLQTGYLRVYALLFVLGALLL
HHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGVMRW
HHHHCH
>Mature Secondary Structure 
ALLGTILLPLLGFALLGLFGKRMREPLPGVLASGLVLASFLLGAGLLLSGGARFQAEWL
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCC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HHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9020134