Click here to switch to the map view.

The map label for this gene is nqo4

Identifier: 55980056

GI number: 55980056

Start: 85284

End: 86513

Strand: Direct

Name: nqo4

Synonym: TTHA0087

Alternate gene names: 55980056

Gene position: 85284-86513 (Clockwise)

Preceding gene: 55980055

Following gene: 55980057

Centisome position: 4.61

GC content: 66.02

Gene sequence:

>1230_bases
ATGAGGGAAGAGTTCCTGGAGGAAATCCCCCTAGACGCCCCCCCGGAGGAGGCCAAGGAGCTCCGCACCGAGGTGATGAC
CCTGAACGTGGGCCCGCAGCACCCCTCCACCCACGGGGTGCTCCGCCTCATGGTGACCCTCTCCGGGGAGGAGGTCCTGG
AGGTGGTGCCCCACATCGGCTACCTCCACACGGGCTTTGAGAAGACCATGGAGCACCGCACCTACCTCCAGAACATCACC
TACACGCCCCGGATGGACTACCTCCACTCCTTCGCCCACGACCTGGCCTACGCCCTGGCGGTGGAGAAGCTTTTGGGGGC
CGTGGTGCCGCCTAGGGCGGAGACCATAAGGGTCATCCTCAACGAGCTCTCCCGCCTGGCGAGCCACCTGGTCTTCCTGG
GGACGGGGCTTTTGGACCTCGGGGCCCTCACCCCCTTCTTCTACGCCTTCCGCGAGCGCGAGACCATCCTGGACCTCTTT
GAGTGGGTCACGGGCCAGCGCTTCCACCACAACTACATCCGCATCGGCGGGGTGAAGGAGGACCTGCCGGAGGAGTTCGT
CCCCGAGCTCAAGAAGCTCCTTGAAGTCCTCCCCCACCGCATAGACGAGTACGAGGCCCTCTTCGCCGAAAGCCCCATCT
TCTACGAAAGGGCCCGGGGCGTGGGGGTGATCCCGCCCGAGGTGGCCATTGACCTCGGCCTCACCGGGGGGTCTCTAAGG
GCGAGCGGGGTGAACTACGACGTGCGCAAGGCCTACCCCTACTCGGGCTACGAGACCTACACCTTTGACGTGCCCCTGGG
GGAGAGGGGGGACGTGTTTGACCGGATGCTCGTCCGCATCCGGGAGATGCGGGAGTCGGTCAAGATCATCAAGCAGGCCC
TGGAGCGCTTAGAACCGGGCCCCGTCCGCGACCCCAACCCCCAGATCACCCCGCCCCCCCGCCACCTCCTGGAGACCTCC
ATGGAGGCGGTCATCTACCACTTCAAGCACTACACCGAGGGCTTCCACCCCCCCAAGGGGGAGGTCTACGTGCCCACGGA
GTCGGCCCGGGGGGAACTCGGCTACTACATCGTCTCCGACGGCGGCTCCATGCCCTACCGGGTCAAGGTGCGGGCGCCGA
GCTTCGTCAACCTGCAAAGCCTCCCCTACGCCTGCAAGGGGGAGCAGGTCCCCGACATGGTGGCCATCATCGCCAGCCTA
GACCCCGTGATGGGGGACGTGGACCGCTAA

Upstream 100 bases:

>100_bases
TCACCGGCAAGGACCCCGGCCTCACCTTCTACAAGGGCGGGAGCCGCAAGGGCTACAGGTCCCTTTGGGCCGACCTGAAG
AAGGCCCGGGAGGTTAAAGG

Downstream 100 bases:

>100_bases
GGGAGGGAAGATGGGGTTCTTTGACGACAAGCAGGACTTTCTGGAGGAGACCTTCGCCAAGTACCCGCCGGAAGGGCGCC
GCGCCGCCATCATGCCCCTC

Product: NADH-quinone oxidoreductase subunit 4

Products: NA

Alternate protein names: NADH dehydrogenase I chain 4; NDH-1 subunit 4

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MREEFLEEIPLDAPPEEAKELRTEVMTLNVGPQHPSTHGVLRLMVTLSGEEVLEVVPHIGYLHTGFEKTMEHRTYLQNIT
YTPRMDYLHSFAHDLAYALAVEKLLGAVVPPRAETIRVILNELSRLASHLVFLGTGLLDLGALTPFFYAFRERETILDLF
EWVTGQRFHHNYIRIGGVKEDLPEEFVPELKKLLEVLPHRIDEYEALFAESPIFYERARGVGVIPPEVAIDLGLTGGSLR
ASGVNYDVRKAYPYSGYETYTFDVPLGERGDVFDRMLVRIREMRESVKIIKQALERLEPGPVRDPNPQITPPPRHLLETS
MEAVIYHFKHYTEGFHPPKGEVYVPTESARGELGYYIVSDGGSMPYRVKVRAPSFVNLQSLPYACKGEQVPDMVAIIASL
DPVMGDVDR

Sequences:

>Translated_409_residues
MREEFLEEIPLDAPPEEAKELRTEVMTLNVGPQHPSTHGVLRLMVTLSGEEVLEVVPHIGYLHTGFEKTMEHRTYLQNIT
YTPRMDYLHSFAHDLAYALAVEKLLGAVVPPRAETIRVILNELSRLASHLVFLGTGLLDLGALTPFFYAFRERETILDLF
EWVTGQRFHHNYIRIGGVKEDLPEEFVPELKKLLEVLPHRIDEYEALFAESPIFYERARGVGVIPPEVAIDLGLTGGSLR
ASGVNYDVRKAYPYSGYETYTFDVPLGERGDVFDRMLVRIREMRESVKIIKQALERLEPGPVRDPNPQITPPPRHLLETS
MEAVIYHFKHYTEGFHPPKGEVYVPTESARGELGYYIVSDGGSMPYRVKVRAPSFVNLQSLPYACKGEQVPDMVAIIASL
DPVMGDVDR
>Mature_409_residues
MREEFLEEIPLDAPPEEAKELRTEVMTLNVGPQHPSTHGVLRLMVTLSGEEVLEVVPHIGYLHTGFEKTMEHRTYLQNIT
YTPRMDYLHSFAHDLAYALAVEKLLGAVVPPRAETIRVILNELSRLASHLVFLGTGLLDLGALTPFFYAFRERETILDLF
EWVTGQRFHHNYIRIGGVKEDLPEEFVPELKKLLEVLPHRIDEYEALFAESPIFYERARGVGVIPPEVAIDLGLTGGSLR
ASGVNYDVRKAYPYSGYETYTFDVPLGERGDVFDRMLVRIREMRESVKIIKQALERLEPGPVRDPNPQITPPPRHLLETS
MEAVIYHFKHYTEGFHPPKGEVYVPTESARGELGYYIVSDGGSMPYRVKVRAPSFVNLQSLPYACKGEQVPDMVAIIASL
DPVMGDVDR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every

COG id: COG0649

COG function: function code C; NADH:ubiquinone oxidoreductase 49 kD subunit 7

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 49 kDa subunit family

Homologues:

Organism=Homo sapiens, GI4758786, Length=391, Percent_Identity=46.2915601023018, Blast_Score=363, Evalue=1e-100,
Organism=Homo sapiens, GI260898743, Length=381, Percent_Identity=45.9317585301837, Blast_Score=352, Evalue=4e-97,
Organism=Escherichia coli, GI145693162, Length=409, Percent_Identity=41.320293398533, Blast_Score=335, Evalue=5e-93,
Organism=Escherichia coli, GI1789076, Length=383, Percent_Identity=30.2872062663185, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1788832, Length=395, Percent_Identity=28.8607594936709, Blast_Score=145, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI17555284, Length=393, Percent_Identity=43.0025445292621, Blast_Score=367, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17568379, Length=393, Percent_Identity=43.0025445292621, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24638644, Length=400, Percent_Identity=43, Blast_Score=365, Evalue=1e-101,
Organism=Drosophila melanogaster, GI221459469, Length=396, Percent_Identity=44.1919191919192, Blast_Score=357, Evalue=6e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NQO4_THET8 (Q56220)

Other databases:

- EMBL:   U52917
- EMBL:   AP008226
- PIR:   T11901
- RefSeq:   YP_143353.1
- PDB:   2FUG
- PDB:   3I9V
- PDB:   3IAM
- PDB:   3IAS
- PDB:   3M9S
- PDBsum:   2FUG
- PDBsum:   3I9V
- PDBsum:   3IAM
- PDBsum:   3IAS
- PDBsum:   3M9S
- ProteinModelPortal:   Q56220
- SMR:   Q56220
- STRING:   Q56220
- GeneID:   3168206
- GenomeReviews:   AP008226_GR
- KEGG:   ttj:TTHA0087
- NMPDR:   fig|300852.3.peg.118
- eggNOG:   COG0649
- HOGENOM:   HBG459705
- OMA:   WIQNIPL
- ProtClustDB:   CLSK445104
- BioCyc:   TTHE300852:TTHA0087-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01358
- InterPro:   IPR010219
- InterPro:   IPR001135
- InterPro:   IPR014029
- InterPro:   IPR022885
- TIGRFAMs:   TIGR01962

Pfam domain/function: PF00346 Complex1_49kDa

EC number: =1.6.99.5

Molecular weight: Translated: 46372; Mature: 46372

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00535 COMPLEX1_49K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREEFLEEIPLDAPPEEAKELRTEVMTLNVGPQHPSTHGVLRLMVTLSGEEVLEVVPHIG
CCHHHHHHCCCCCCHHHHHHHHHHHEEEECCCCCCCHHHHHHEEECCCCHHHHHHHHCCC
YLHTGFEKTMEHRTYLQNITYTPRMDYLHSFAHDLAYALAVEKLLGAVVPPRAETIRVIL
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
NELSRLASHLVFLGTGLLDLGALTPFFYAFRERETILDLFEWVTGQRFHHNYIRIGGVKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHH
DLPEEFVPELKKLLEVLPHRIDEYEALFAESPIFYERARGVGVIPPEVAIDLGLTGGSLR
HCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCHHEEEECCCCCCCE
ASGVNYDVRKAYPYSGYETYTFDVPLGERGDVFDRMLVRIREMRESVKIIKQALERLEPG
ECCCCEEHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PVRDPNPQITPPPRHLLETSMEAVIYHFKHYTEGFHPPKGEVYVPTESARGELGYYIVSD
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCEEEEEC
GGSMPYRVKVRAPSFVNLQSLPYACKGEQVPDMVAIIASLDPVMGDVDR
CCCCCEEEEECCCCEECHHCCCCCCCCCCCHHHHHHHHHHCHHHCCCCC
>Mature Secondary Structure
MREEFLEEIPLDAPPEEAKELRTEVMTLNVGPQHPSTHGVLRLMVTLSGEEVLEVVPHIG
CCHHHHHHCCCCCCHHHHHHHHHHHEEEECCCCCCCHHHHHHEEECCCCHHHHHHHHCCC
YLHTGFEKTMEHRTYLQNITYTPRMDYLHSFAHDLAYALAVEKLLGAVVPPRAETIRVIL
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
NELSRLASHLVFLGTGLLDLGALTPFFYAFRERETILDLFEWVTGQRFHHNYIRIGGVKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHH
DLPEEFVPELKKLLEVLPHRIDEYEALFAESPIFYERARGVGVIPPEVAIDLGLTGGSLR
HCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCHHEEEECCCCCCCE
ASGVNYDVRKAYPYSGYETYTFDVPLGERGDVFDRMLVRIREMRESVKIIKQALERLEPG
ECCCCEEHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PVRDPNPQITPPPRHLLETSMEAVIYHFKHYTEGFHPPKGEVYVPTESARGELGYYIVSD
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCEEEEEC
GGSMPYRVKVRAPSFVNLQSLPYACKGEQVPDMVAIIASLDPVMGDVDR
CCCCCEEEEECCCCEECHHCCCCCCCCCCCHHHHHHHHHHCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9020134