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The map label for this gene is topA [H]

Identifier: 55980043

GI number: 55980043

Start: 68753

End: 71245

Strand: Reverse

Name: topA [H]

Synonym: TTHA0074

Alternate gene names: 55980043

Gene position: 71245-68753 (Counterclockwise)

Preceding gene: 55980047

Following gene: 55980042

Centisome position: 3.85

GC content: 72.4

Gene sequence:

>2493_bases
ATGCCCAAGAGCGCCAAGCGCCGCGCGAGCCAGCCCACCGGCGCGGAGACCCTGGTGGTGGTGGAGTCCCCGGCCAAGGC
CCGGAGCATCCAAAAGATGCTGGGGCCGGGCTACGAGGTCCGGGCGAGCCTAGGGCACGTGGTGGACCTCCCCGAGCGGA
GCCTGGGGGTGGACGTGGAGCGGGACTTCGCCCCCACCTACGAGGTGAAGAAGGACAAGAAGCCCGTGGTGGAGGAGCTG
AAGCGGGCGGCGGAGGGCAAGCGCCTGCTCATCGCCACCGACCCCGACCGGGAGGGAGAGGCCATCGGCTGGCACGTGGC
CCGCCTCCTGGGGCGGGACCCCAAGGAGCCCCTCCGGGTGGAGTTCCACGAGATCACCCCCAGGGTGGTCCGCCAGGCGG
TGGAGGCCCCGCGGCCCATTGACCAGAACCTGGTGGACGCCCAGCAGGCCCGGCGCGTCCTGGACCGCCTCCTCGGGTAC
AACCTCTCCCCCCTCCTCTCCCTGGAGTTCCGCAAGCGGGCCCTCTCGGCCGGCCGGGTGCAGAGCGTGGCCTTGAGGCT
CGTGGTGGAGCGGGAGGAGGCCATTGAGGCCTTCCGGCCCGAGGAGTACTGGGTCCTCCGGGGCGTCTTCGCCGTGGAGG
GCGGGGAGGCCCCCCTGGTGGCCACGCTCCACGAGGTGGAGGGGAAGCGCCTCTGGACGGGGCAGGGGGAGAAGGAGGGC
CGGCTCCACCTCGCCTCGGAGGCCGAGGCCCATGCCCTGGCCGAGGCCCTGCGGAAGGGGAGCTACCGGGTGGCCCAGGT
GGAGGTGCGGGAGCGGCGCAAGGCCCCCCCGCCCCCCTTCACCACCTCCACCCTCCAGCAGGCCGCCTCGAGCCGCTTGG
GCTACACCGCCGCCCGCACCATGCGCATCGCCCAGCGCCTCTACGAGGGGGTGGACCTGCCGGAGGGGACCGTGGGCCTC
ATCACCTACATGCGCACGGACTCGGTGCGGGTGGCCCCCGAGGCGGTGGCGGCGGCCCGGGAGGTGATCGGGAAGGCCTT
CGGCCCGGAGTACCTGCCGGAGGCCCCGCGGGCCTATAAGAACCGCAGGGAAGGGGTCCAGGACGCCCACGAGGCCATCC
GCCCCACCGACCCCAAAAGGACCCCGGAAAGCGTCCGCCCCTACCTGTCCGAGGAGGAGTACCGCCTCTACGAGCTCATC
TGGCGCCGCTTCCTGGCGAGCCAGATGCGGGACGCCCTCTACGAGCAGACGGCGGTCACCCTGGCCGACCTCGAGGGCCG
CTACGCCTTCCGCGCCTCGGGCTCGGTGTTGCGCTTTGAGGGGTACCTCCGGGCCTGGGGGCGGGACGCCGAGGAGGAGG
AGGAGCCCCCCGTCCCCGCGGTGCCCGAGGGGGTTCCCGCCCGGCTTGAGGAGGTCCTGGAGGAGCGGCGCTTCACCGAG
CCCCCGCCCCGCTACACCGACGCCAGCCTGGTGAAGACCATGGAGGAGCTGGGGATCGGCCGCCCTTCCACCTACGCCCC
TACCCTCGAGACCCTGGAAAAGCGGGGGTACGTGGCCCGAAAAGGGCGGACCCTTTTCCCCACCCCCCTGGGCCGCCAGG
TCACCGCCTACCTGCGGGAAAGGTTCCCCCAGGTGGTGGCCTACGAGTTCACCGCCCGGATGGAGGAGCGGCTGGACGAG
GTGGAGGAGGGGCGCGTCCCCTGGCCCAAGGTGGTCTGGGAGTTCTACGAGCCCTTTTTGGGGGAGCTCGCCCAGGTGCC
CAAGAAGACCTGCCCCCGGTGCGGCAGGCCCTTGGAGCTCAAGGTGAGCCGCTACGGCCAGTTCCTGGGGTGCACGGGCT
ACCCGGAGTGCACCTACACCGAGCCCCTGGAAAGGCGGGAGGCGGAGCCCATCGGGGAGGCCTGCCCCAAGTGCGGCCGC
CCCCTCCTCAGGAAGGAGGGCCGCTACGGCACCTTCATCGCCTGCTCCGGCTACCCCGAGTGCGACTACACCCGGGACGA
CGGGACCCCCACGGGCCACACCTGCCCCAAGTGCGGGGGCCGGGTTCTGGAAAAGCGGAGCCGGAAGGGCAGGCCCTACT
ACAAGTGCGAGAACAACGCTTGCGACTTCCTCTCCTTCTACCCCCTCCTGGAGGAGGCCTGCCCCGCCTGCGGCTGGCCT
TTGGTGAAGAAGGGGGAGAAGGCGCTCTGCCTCAACCCCGCCTGCCCGGAGCACGAGCCGAGCCTCCTTCCCGCCCCCAA
GGAGGCCAAGAAAGGGCGGTCCCGGGGGAAGGGCAAGCCCCCCCTCGCCCCCCCTAAGGACTGGGAGGAGCTCGTCCCCT
TCCTCCCCGGGCTTTCCCCCGAGGAGCGCCGGGCGGTGGAGGCCCTGGCCCGGGGGGAGGCCCTGGCCCGGGGGGAGGCC
CTGGCCGAGGAGGAGGCGCGGCTCGCCCGGCGGGCCCTCTTCAAGCTCCGCATGCGCAAGGGCAAGGCCCGGAAGGAGCC
GGTCCGGGCGTGA

Upstream 100 bases:

>100_bases
CTTTGGCCTGGGATGTTGCCCGTAGCCTCCAGACTACCACAAGACCTCGAGGGCCCGTGGGGTTGACAAGCCGCGGGAGG
AGGCCCCTAAATAGGGGGCG

Downstream 100 bases:

>100_bases
TGGCGAGCTTCAAGCTCCACCTCCTGCGCCTCGCCCGGGAGGGCCAGGTGGAGGTCCCGGGGGTGCCGGGGCTTAGGGCC
TACACCTTGAGGGAGCGGGT

Product: DNA topoisomerase IA

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 830; Mature: 829

Protein sequence:

>830_residues
MPKSAKRRASQPTGAETLVVVESPAKARSIQKMLGPGYEVRASLGHVVDLPERSLGVDVERDFAPTYEVKKDKKPVVEEL
KRAAEGKRLLIATDPDREGEAIGWHVARLLGRDPKEPLRVEFHEITPRVVRQAVEAPRPIDQNLVDAQQARRVLDRLLGY
NLSPLLSLEFRKRALSAGRVQSVALRLVVEREEAIEAFRPEEYWVLRGVFAVEGGEAPLVATLHEVEGKRLWTGQGEKEG
RLHLASEAEAHALAEALRKGSYRVAQVEVRERRKAPPPPFTTSTLQQAASSRLGYTAARTMRIAQRLYEGVDLPEGTVGL
ITYMRTDSVRVAPEAVAAAREVIGKAFGPEYLPEAPRAYKNRREGVQDAHEAIRPTDPKRTPESVRPYLSEEEYRLYELI
WRRFLASQMRDALYEQTAVTLADLEGRYAFRASGSVLRFEGYLRAWGRDAEEEEEPPVPAVPEGVPARLEEVLEERRFTE
PPPRYTDASLVKTMEELGIGRPSTYAPTLETLEKRGYVARKGRTLFPTPLGRQVTAYLRERFPQVVAYEFTARMEERLDE
VEEGRVPWPKVVWEFYEPFLGELAQVPKKTCPRCGRPLELKVSRYGQFLGCTGYPECTYTEPLERREAEPIGEACPKCGR
PLLRKEGRYGTFIACSGYPECDYTRDDGTPTGHTCPKCGGRVLEKRSRKGRPYYKCENNACDFLSFYPLLEEACPACGWP
LVKKGEKALCLNPACPEHEPSLLPAPKEAKKGRSRGKGKPPLAPPKDWEELVPFLPGLSPEERRAVEALARGEALARGEA
LAEEEARLARRALFKLRMRKGKARKEPVRA

Sequences:

>Translated_830_residues
MPKSAKRRASQPTGAETLVVVESPAKARSIQKMLGPGYEVRASLGHVVDLPERSLGVDVERDFAPTYEVKKDKKPVVEEL
KRAAEGKRLLIATDPDREGEAIGWHVARLLGRDPKEPLRVEFHEITPRVVRQAVEAPRPIDQNLVDAQQARRVLDRLLGY
NLSPLLSLEFRKRALSAGRVQSVALRLVVEREEAIEAFRPEEYWVLRGVFAVEGGEAPLVATLHEVEGKRLWTGQGEKEG
RLHLASEAEAHALAEALRKGSYRVAQVEVRERRKAPPPPFTTSTLQQAASSRLGYTAARTMRIAQRLYEGVDLPEGTVGL
ITYMRTDSVRVAPEAVAAAREVIGKAFGPEYLPEAPRAYKNRREGVQDAHEAIRPTDPKRTPESVRPYLSEEEYRLYELI
WRRFLASQMRDALYEQTAVTLADLEGRYAFRASGSVLRFEGYLRAWGRDAEEEEEPPVPAVPEGVPARLEEVLEERRFTE
PPPRYTDASLVKTMEELGIGRPSTYAPTLETLEKRGYVARKGRTLFPTPLGRQVTAYLRERFPQVVAYEFTARMEERLDE
VEEGRVPWPKVVWEFYEPFLGELAQVPKKTCPRCGRPLELKVSRYGQFLGCTGYPECTYTEPLERREAEPIGEACPKCGR
PLLRKEGRYGTFIACSGYPECDYTRDDGTPTGHTCPKCGGRVLEKRSRKGRPYYKCENNACDFLSFYPLLEEACPACGWP
LVKKGEKALCLNPACPEHEPSLLPAPKEAKKGRSRGKGKPPLAPPKDWEELVPFLPGLSPEERRAVEALARGEALARGEA
LAEEEARLARRALFKLRMRKGKARKEPVRA
>Mature_829_residues
PKSAKRRASQPTGAETLVVVESPAKARSIQKMLGPGYEVRASLGHVVDLPERSLGVDVERDFAPTYEVKKDKKPVVEELK
RAAEGKRLLIATDPDREGEAIGWHVARLLGRDPKEPLRVEFHEITPRVVRQAVEAPRPIDQNLVDAQQARRVLDRLLGYN
LSPLLSLEFRKRALSAGRVQSVALRLVVEREEAIEAFRPEEYWVLRGVFAVEGGEAPLVATLHEVEGKRLWTGQGEKEGR
LHLASEAEAHALAEALRKGSYRVAQVEVRERRKAPPPPFTTSTLQQAASSRLGYTAARTMRIAQRLYEGVDLPEGTVGLI
TYMRTDSVRVAPEAVAAAREVIGKAFGPEYLPEAPRAYKNRREGVQDAHEAIRPTDPKRTPESVRPYLSEEEYRLYELIW
RRFLASQMRDALYEQTAVTLADLEGRYAFRASGSVLRFEGYLRAWGRDAEEEEEPPVPAVPEGVPARLEEVLEERRFTEP
PPRYTDASLVKTMEELGIGRPSTYAPTLETLEKRGYVARKGRTLFPTPLGRQVTAYLRERFPQVVAYEFTARMEERLDEV
EEGRVPWPKVVWEFYEPFLGELAQVPKKTCPRCGRPLELKVSRYGQFLGCTGYPECTYTEPLERREAEPIGEACPKCGRP
LLRKEGRYGTFIACSGYPECDYTRDDGTPTGHTCPKCGGRVLEKRSRKGRPYYKCENNACDFLSFYPLLEEACPACGWPL
VKKGEKALCLNPACPEHEPSLLPAPKEAKKGRSRGKGKPPLAPPKDWEELVPFLPGLSPEERRAVEALARGEALARGEAL
AEEEARLARRALFKLRMRKGKARKEPVRA

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=662, Percent_Identity=27.6435045317221, Blast_Score=143, Evalue=6e-34,
Organism=Homo sapiens, GI4507635, Length=686, Percent_Identity=24.0524781341108, Blast_Score=115, Evalue=1e-25,
Organism=Escherichia coli, GI1787529, Length=760, Percent_Identity=37.8947368421053, Blast_Score=448, Evalue=1e-127,
Organism=Escherichia coli, GI1788061, Length=544, Percent_Identity=28.6764705882353, Blast_Score=140, Evalue=4e-34,
Organism=Escherichia coli, GI48994931, Length=163, Percent_Identity=36.8098159509202, Blast_Score=107, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI32563869, Length=653, Percent_Identity=25.2679938744257, Blast_Score=140, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17555378, Length=532, Percent_Identity=27.0676691729323, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24585251, Length=648, Percent_Identity=26.2345679012346, Blast_Score=146, Evalue=6e-35,
Organism=Drosophila melanogaster, GI24640096, Length=514, Percent_Identity=25.2918287937743, Blast_Score=120, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 93445; Mature: 93314

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKSAKRRASQPTGAETLVVVESPAKARSIQKMLGPGYEVRASLGHVVDLPERSLGVDVE
CCCCHHHHCCCCCCCCEEEEECCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHCCCCC
RDFAPTYEVKKDKKPVVEELKRAAEGKRLLIATDPDREGEAIGWHVARLLGRDPKEPLRV
CCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCHHH
EFHEITPRVVRQAVEAPRPIDQNLVDAQQARRVLDRLLGYNLSPLLSLEFRKRALSAGRV
HHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
QSVALRLVVEREEAIEAFRPEEYWVLRGVFAVEGGEAPLVATLHEVEGKRLWTGQGEKEG
HHHHHHHHHHHHHHHHHHCCCHHEEEEEEEEECCCCCCHHHHHHHHCCCEEECCCCCCCC
RLHLASEAEAHALAEALRKGSYRVAQVEVRERRKAPPPPFTTSTLQQAASSRLGYTAART
CEEECCHHHHHHHHHHHHCCCCEEEEHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHH
MRIAQRLYEGVDLPEGTVGLITYMRTDSVRVAPEAVAAAREVIGKAFGPEYLPEAPRAYK
HHHHHHHHHCCCCCCCCEEEEEEEECCCCEECHHHHHHHHHHHHHHCCCCCCCCCHHHHH
NRREGVQDAHEAIRPTDPKRTPESVRPYLSEEEYRLYELIWRRFLASQMRDALYEQTAVT
HHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LADLEGRYAFRASGSVLRFEGYLRAWGRDAEEEEEPPVPAVPEGVPARLEEVLEERRFTE
HHHCCCCEEEECCCCEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
PPPRYTDASLVKTMEELGIGRPSTYAPTLETLEKRGYVARKGRTLFPTPLGRQVTAYLRE
CCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCEECCCCEECCCCCCHHHHHHHHH
RFPQVVAYEFTARMEERLDEVEEGRVPWPKVVWEFYEPFLGELAQVPKKTCPRCGRPLEL
HCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCEE
KVSRYGQFLGCTGYPECTYTEPLERREAEPIGEACPKCGRPLLRKEGRYGTFIACSGYPE
EHHHCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHCCHHHHCCCCCCEEEEECCCCC
CDYTRDDGTPTGHTCPKCGGRVLEKRSRKGRPYYKCENNACDFLSFYPLLEEACPACGWP
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHCCCCCCH
LVKKGEKALCLNPACPEHEPSLLPAPKEAKKGRSRGKGKPPLAPPKDWEELVPFLPGLSP
HHHCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCH
EERRAVEALARGEALARGEALAEEEARLARRALFKLRMRKGKARKEPVRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
PKSAKRRASQPTGAETLVVVESPAKARSIQKMLGPGYEVRASLGHVVDLPERSLGVDVE
CCCHHHHCCCCCCCCEEEEECCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHCCCCC
RDFAPTYEVKKDKKPVVEELKRAAEGKRLLIATDPDREGEAIGWHVARLLGRDPKEPLRV
CCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCHHH
EFHEITPRVVRQAVEAPRPIDQNLVDAQQARRVLDRLLGYNLSPLLSLEFRKRALSAGRV
HHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
QSVALRLVVEREEAIEAFRPEEYWVLRGVFAVEGGEAPLVATLHEVEGKRLWTGQGEKEG
HHHHHHHHHHHHHHHHHHCCCHHEEEEEEEEECCCCCCHHHHHHHHCCCEEECCCCCCCC
RLHLASEAEAHALAEALRKGSYRVAQVEVRERRKAPPPPFTTSTLQQAASSRLGYTAART
CEEECCHHHHHHHHHHHHCCCCEEEEHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHH
MRIAQRLYEGVDLPEGTVGLITYMRTDSVRVAPEAVAAAREVIGKAFGPEYLPEAPRAYK
HHHHHHHHHCCCCCCCCEEEEEEEECCCCEECHHHHHHHHHHHHHHCCCCCCCCCHHHHH
NRREGVQDAHEAIRPTDPKRTPESVRPYLSEEEYRLYELIWRRFLASQMRDALYEQTAVT
HHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LADLEGRYAFRASGSVLRFEGYLRAWGRDAEEEEEPPVPAVPEGVPARLEEVLEERRFTE
HHHCCCCEEEECCCCEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
PPPRYTDASLVKTMEELGIGRPSTYAPTLETLEKRGYVARKGRTLFPTPLGRQVTAYLRE
CCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCEECCCCEECCCCCCHHHHHHHHH
RFPQVVAYEFTARMEERLDEVEEGRVPWPKVVWEFYEPFLGELAQVPKKTCPRCGRPLEL
HCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCEE
KVSRYGQFLGCTGYPECTYTEPLERREAEPIGEACPKCGRPLLRKEGRYGTFIACSGYPE
EHHHCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHCCHHHHCCCCCCEEEEECCCCC
CDYTRDDGTPTGHTCPKCGGRVLEKRSRKGRPYYKCENNACDFLSFYPLLEEACPACGWP
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHCCCCCCH
LVKKGEKALCLNPACPEHEPSLLPAPKEAKKGRSRGKGKPPLAPPKDWEELVPFLPGLSP
HHHCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCH
EERRAVEALARGEALARGEALAEEEARLARRALFKLRMRKGKARKEPVRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]