Click here to switch to the map view.

The map label for this gene is lpxD

Identifier: 54295783

GI number: 54295783

Start: 3278798

End: 3279829

Strand: Reverse

Name: lpxD

Synonym: lpl2873

Alternate gene names: 54295783

Gene position: 3279829-3278798 (Counterclockwise)

Preceding gene: 54295784

Following gene: 54295781

Centisome position: 98.03

GC content: 41.76

Gene sequence:

>1032_bases
ATGTCAAATTACCAATTTACAAAACCTGCAGGACCATTTTATTTAGCGGAATTAGCCGAGATTTCTGGCTCTACCCTGTA
TGAAGGCAAAGGAGAAGCATTTACAGTTTCTGGTTTGGCAAAACTTTCGGAAGCGACAACCCATAATTTGGTAATGTTGC
ATCAAAAAAAATACCTGAAAGAATTAAAAAATACAGCCGCTCGTGCTTGTATTATCGGGCCAGAGTATGTGAAATTTGCG
CCTGATAGCATGTATTTGCTGGTACACCCAAATCCGTATAAAGCTTTTGCTTTAATTGCTCAAGCTTTTTATCCTTCAGA
GAAACCAGCCGGGTTTATTGCTTCCTCCGCAGCAATCGAAACCTCTGCGGTAATTGGCTCAAACTGTTATATTGCTCATG
GAGTTTATATAGGCAATAACGCGAAAATTGGTTCCGGATGCCAGATTGGAGTGAATACTTATATTGGTGATGGTGTAACC
ATAGGCGACGATTGCCTTATAGAAGACAATGTCAGCATCAGGCATGCTGTTATAGGGAAGCATGTAGTTATTTATCCTGG
TGCCCGTATAGGCCAGGATGGTTTTGGATTTGCCAGTGATGCGAGTGGTCATTATAAAATTCCTCATGCAGGAGGGGTCA
TTATTGGCAATCATGTTGAAATTGGCGCTAATACCTGTATTGATCGAGGTTCTCTGGATAATACTGTGATTGAAGACTGG
TGTCGTTTGGATAATCTTGTGCAGGTTGGGCATAATGTCAAGATTGGTAAAGGGTCTATTATAGTTGCTCAAGTTGGAAT
TGCTGGAAGTACAGAGCTTGGGGAGTATGTTACTCTGGCTGGACAAGCTGGGGTGATTGGGCACTTGAAGATTGGCAAAG
GTGCCACTGTTTTAGCAAGCGGTAAAGTCTATAAAAATGTAAAATCTGGAGACAGAGTCGGAGGCCACCCGGCTGTATCA
ATTAGTGATTGGCAAAAACAAATCCGATTTTTAAAAACAGCGATCAAGCCGAAGAAGTCTCCAAAATCTTGA

Upstream 100 bases:

>100_bases
GCTTAAAAAAGAGTTTCAAAAAAGCATGGATGTAAATTATATTCTCAAATTCCTTGAAAATTCTAAACGTAGCTTTTATC
GTTAATTTTTTAGAGTGTGT

Downstream 100 bases:

>100_bases
GGATGGATAAAGCAGCTTTTTCATTGGCATTTTGCCTTAATGTGTGATGAATGGCCGTAAATTGTTTATTTAAGTTGCTT
TGACTGGGGCAGGCAAGCAA

Product: UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase

Products: UDP-2,3-Bis(3-Hydroxytetradecanoyl)Glucosamine; [Acyl-Carrier Protein]. [C]

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MSNYQFTKPAGPFYLAELAEISGSTLYEGKGEAFTVSGLAKLSEATTHNLVMLHQKKYLKELKNTAARACIIGPEYVKFA
PDSMYLLVHPNPYKAFALIAQAFYPSEKPAGFIASSAAIETSAVIGSNCYIAHGVYIGNNAKIGSGCQIGVNTYIGDGVT
IGDDCLIEDNVSIRHAVIGKHVVIYPGARIGQDGFGFASDASGHYKIPHAGGVIIGNHVEIGANTCIDRGSLDNTVIEDW
CRLDNLVQVGHNVKIGKGSIIVAQVGIAGSTELGEYVTLAGQAGVIGHLKIGKGATVLASGKVYKNVKSGDRVGGHPAVS
ISDWQKQIRFLKTAIKPKKSPKS

Sequences:

>Translated_343_residues
MSNYQFTKPAGPFYLAELAEISGSTLYEGKGEAFTVSGLAKLSEATTHNLVMLHQKKYLKELKNTAARACIIGPEYVKFA
PDSMYLLVHPNPYKAFALIAQAFYPSEKPAGFIASSAAIETSAVIGSNCYIAHGVYIGNNAKIGSGCQIGVNTYIGDGVT
IGDDCLIEDNVSIRHAVIGKHVVIYPGARIGQDGFGFASDASGHYKIPHAGGVIIGNHVEIGANTCIDRGSLDNTVIEDW
CRLDNLVQVGHNVKIGKGSIIVAQVGIAGSTELGEYVTLAGQAGVIGHLKIGKGATVLASGKVYKNVKSGDRVGGHPAVS
ISDWQKQIRFLKTAIKPKKSPKS
>Mature_342_residues
SNYQFTKPAGPFYLAELAEISGSTLYEGKGEAFTVSGLAKLSEATTHNLVMLHQKKYLKELKNTAARACIIGPEYVKFAP
DSMYLLVHPNPYKAFALIAQAFYPSEKPAGFIASSAAIETSAVIGSNCYIAHGVYIGNNAKIGSGCQIGVNTYIGDGVTI
GDDCLIEDNVSIRHAVIGKHVVIYPGARIGQDGFGFASDASGHYKIPHAGGVIIGNHVEIGANTCIDRGSLDNTVIEDWC
RLDNLVQVGHNVKIGKGSIIVAQVGIAGSTELGEYVTLAGQAGVIGHLKIGKGATVLASGKVYKNVKSGDRVGGHPAVSI
SDWQKQIRFLKTAIKPKKSPKS

Specific function: Lipid A biosynthesis; third step. [C]

COG id: COG1044

COG function: function code M; UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family. LpxD subfamily

Homologues:

Organism=Escherichia coli, GI1786376, Length=339, Percent_Identity=34.8082595870207, Blast_Score=169, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LPXD2_LEGPL (Q5WSK5)

Other databases:

- EMBL:   CR628337
- RefSeq:   YP_128198.1
- HSSP:   P07464
- ProteinModelPortal:   Q5WSK5
- SMR:   Q5WSK5
- STRING:   Q5WSK5
- GeneID:   3113438
- GenomeReviews:   CR628337_GR
- KEGG:   lpf:lpl2873
- NMPDR:   fig|297245.3.peg.1163
- LegioList:   lpl2873
- eggNOG:   COG1044
- HOGENOM:   HBG469615
- OMA:   IAQAFYP
- PhylomeDB:   Q5WSK5
- ProtClustDB:   PRK00892
- BioCyc:   LPNE297245:LPL2873-MONOMER
- HAMAP:   MF_00523
- InterPro:   IPR001451
- InterPro:   IPR011004
- InterPro:   IPR007691
- InterPro:   IPR020573
- TIGRFAMs:   TIGR01853

Pfam domain/function: PF00132 Hexapep; PF04613 LpxD; SSF51161 Trimer_LpxA_like

EC number: 2.3.1.- [C]

Molecular weight: Translated: 36231; Mature: 36100

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNYQFTKPAGPFYLAELAEISGSTLYEGKGEAFTVSGLAKLSEATTHNLVMLHQKKYLK
CCCCCCCCCCCCHHHHHHHHCCCCEEECCCCCEEEEHHHHHHHHCCCCCEEEEHHHHHHH
ELKNTAARACIIGPEYVKFAPDSMYLLVHPNPYKAFALIAQAFYPSEKPAGFIASSAAIE
HHHHHHHEEEEECCHHEEECCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCEEECCCCEE
TSAVIGSNCYIAHGVYIGNNAKIGSGCQIGVNTYIGDGVTIGDDCLIEDNVSIRHAVIGK
EHEEECCCEEEEEEEEECCCCCCCCCEEEEEEEEECCCEEECCCEEEECCCCEEEEECCC
HVVIYPGARIGQDGFGFASDASGHYKIPHAGGVIIGNHVEIGANTCIDRGSLDNTVIEDW
EEEEECCCEECCCCCCCCCCCCCCEECCCCCCEEECCEEEECCCCCCCCCCCCHHHHHHH
CRLDNLVQVGHNVKIGKGSIIVAQVGIAGSTELGEYVTLAGQAGVIGHLKIGKGATVLAS
HHHHHHHHCCCCEEECCCCEEEEEECCCCCCCCCCEEEEECCCCEEEEEEECCCCEEEEC
GKVYKNVKSGDRVGGHPAVSISDWQKQIRFLKTAIKPKKSPKS
CHHEECCCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SNYQFTKPAGPFYLAELAEISGSTLYEGKGEAFTVSGLAKLSEATTHNLVMLHQKKYLK
CCCCCCCCCCCHHHHHHHHCCCCEEECCCCCEEEEHHHHHHHHCCCCCEEEEHHHHHHH
ELKNTAARACIIGPEYVKFAPDSMYLLVHPNPYKAFALIAQAFYPSEKPAGFIASSAAIE
HHHHHHHEEEEECCHHEEECCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCEEECCCCEE
TSAVIGSNCYIAHGVYIGNNAKIGSGCQIGVNTYIGDGVTIGDDCLIEDNVSIRHAVIGK
EHEEECCCEEEEEEEEECCCCCCCCCEEEEEEEEECCCEEECCCEEEECCCCEEEEECCC
HVVIYPGARIGQDGFGFASDASGHYKIPHAGGVIIGNHVEIGANTCIDRGSLDNTVIEDW
EEEEECCCEECCCCCCCCCCCCCCEECCCCCCEEECCEEEECCCCCCCCCCCCHHHHHHH
CRLDNLVQVGHNVKIGKGSIIVAQVGIAGSTELGEYVTLAGQAGVIGHLKIGKGATVLAS
HHHHHHHHCCCCEEECCCCEEEEEECCCCCCCCCCEEEEECCCCEEEEEEECCCCEEEEC
GKVYKNVKSGDRVGGHPAVSISDWQKQIRFLKTAIKPKKSPKS
CHHEECCCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-3-O-(3-Hydroxytetradecanoyl)Glucosamine; (R)-3-Hydroxytetradecanoyl-[Acyl-Carrier Protein] [C]

Specific reaction: UDP-3-O-(3-Hydroxytetradecanoyl)Glucosamine + (R)-3-Hydroxytetradecanoyl-[Acyl-Carrier Protein] = UDP-2,3-Bis(3-Hydroxytetradecanoyl)Glucosamine + [Acyl-Carrier Protein]. [C]

General reaction: Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA