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The map label for this gene is rne [H]

Identifier: 54295136

GI number: 54295136

Start: 2529623

End: 2531614

Strand: Direct

Name: rne [H]

Synonym: lpl2216

Alternate gene names: 54295136

Gene position: 2529623-2531614 (Clockwise)

Preceding gene: 54295135

Following gene: 54295138

Centisome position: 75.61

GC content: 41.11

Gene sequence:

>1992_bases
ATGGAAAAAATGCTAATCAATGCAATGCAAACAGAAGAAATTCGTGTTGCACTAATTAAAAACAATCAATTATTTGATCT
GGACATAGAATGCCCGGGAGAAATAAAGAAAAAAGGTAATATCTATAAAGCCATTGTAACAAGAAGAGAGCCCAGTCTTG
ATGCTGTTTTCGTTGAATATGGTTCCAAACGCCAAGGTTTCTTGCCCCTAAAAGAAATAGCGCCTGAATATTTAAGCAAG
AATCCGGAAGAGTTTGGAGATGAGAAACCTCCAATTACCAGCCTCATCCGTGAAGGTCAAGAATTACTTATTCAAGTGGA
TAAAGAAGAAAGAGGGAATAAAGGAGCAGCACTGACAACTTTTATTACCCTGGCAGGTTGCTACCTGGTTTTAATGCCCA
ATAACCCTCATTCGGGCGGAATATCAAGACGTATTGAAGGTGATGAGCGTGATGAATTACGCGAAACATTAAATGCTTTA
ACACTCCCTGAAGACATGGGTCTTATTATTCGCACTGCGGGTGTTGGAAAAAGTCTGGAAGAACTGCAAGATGACTTAAA
CATGCTTTGCAATCAGTGGCAATCCATCAAGGAAGCTTATAATTCAGAGCTGGCACCTTGCCTGATTCATCAAGAGGGCG
ATGTCATTATTCGTTCCATTCGTGACAATTTGCGAAAATCCATCAGTGAAATTATTATTGATGATCAAATATCCTATATC
AAAGCAAAACAATACATTGAACGCGTAAAACCAGAATTTTTACCTAATCTAAAACTCTATAACAGCAGCATCCCTCTTTT
TAATTTCTATCAAATTGAAAGCCAAATTGAGACAGCTTATCAGCGACAGGTCATGCTTCCTTCTGGAGGTGCCTTGGTCA
TAGATAGAACTGAAGCTCTGGTATCAATTGATATTAACTCAGCCAAGGCTACAGGTGGCGCCGACATTGAAGCGACTGCA
TTAAATACCAACCTTGAAGCAGCTGATGAAATTGCTCGCCAGCTTCGCCTGCGTGATTTAGGTGGTTTGGTAGTTATTGA
TTTTATTGACATGAGTTCTAGTAAAAATCAAAGAGAGGTAGAAAATCGACTCAAAGAAGCACTGCAGGCTGATAGGGCCA
GAATTCAAGTAGGGCGAATTTCACGCTTTGGCCTTCTTGAAATGTCAAGGCAACGCCTCCGATTATCATTAGGAGAAACT
GCCCAGGAAATTTGTCCACGTTGTGAAGGTAGAGGAACTGTACGCAGTATACAGTCTCATGGCTTATCAATTACCCGCCT
TATCGAAGAAGAAGCGCTCAAGGAAAAAACAGCTGAGATTCAAGTACAATTGCCACCAGAAATGGCTACATTCATTATGA
ATGAAAAACGTAATTTTATCAGGGACATTGAAAAAAGACATGGTGTCAGTGTTGTTGTTATTGCGAATCCTTATATGCAC
CCTCCACAATATACGATTACTCGCCTGAAAGAAGACAACGTAGGCAAATCTAAAAAACCAAGTTACTCATTGATTCAGCA
ACCTGAAGTACATGTTCCCAGCAGTGATAAAGAACCCGTGTCACATGATGAACCTGCTGTGAAATCATTTGCCGAACACA
GGGTCATCAAACACCCTCAAACCAGTTTTATTAAACGATTATGGACCAGTTTATTTGGTGGCCATTCAGTTGCCCACACC
GTAACTCAAGAGCCTGGTAAAAAACATCATCGTGCTCAAAACCAAGGTAAACACCCATCGTCCAAAAGCTCAGGAGGAGA
CAAACGTCAACAAGGCAGCAACGCAAATCGTAGACGTCGTTCAGGAGGAGGCCAGCAGCGTTCTTCATCAGGAAATACAA
ATGCTGCGGCCAGCACACATCAACAACGAAAAAAATCTAACCCGAATCAGCAAAGTCATGGCAGCCAACAAAGCAATCGT
TCAATTCCTGCAAAAAATGAAGGCAACAAAAAAAGGGAAGCCATACTGGCTAAAGAGCAAGAGGAGAAATAG

Upstream 100 bases:

>100_bases
TTAACTTCATGGCAAGAAAACTGTAGAAATGATCATACAGCTATACTGAGCCTTACACTATCTCGATGTAAATGCATGCG
CCCTAAATATGGGGTTATAC

Downstream 100 bases:

>100_bases
GCCGCCATATTAATTATTTGTCTATTCACCTTTTCAATAAGCATTTACCCGCACAATCCAGTTACCTCTGGATTGTGCGG
GTAAAACACAGCACTCTTTC

Product: ribonuclease E

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 663; Mature: 663

Protein sequence:

>663_residues
MEKMLINAMQTEEIRVALIKNNQLFDLDIECPGEIKKKGNIYKAIVTRREPSLDAVFVEYGSKRQGFLPLKEIAPEYLSK
NPEEFGDEKPPITSLIREGQELLIQVDKEERGNKGAALTTFITLAGCYLVLMPNNPHSGGISRRIEGDERDELRETLNAL
TLPEDMGLIIRTAGVGKSLEELQDDLNMLCNQWQSIKEAYNSELAPCLIHQEGDVIIRSIRDNLRKSISEIIIDDQISYI
KAKQYIERVKPEFLPNLKLYNSSIPLFNFYQIESQIETAYQRQVMLPSGGALVIDRTEALVSIDINSAKATGGADIEATA
LNTNLEAADEIARQLRLRDLGGLVVIDFIDMSSSKNQREVENRLKEALQADRARIQVGRISRFGLLEMSRQRLRLSLGET
AQEICPRCEGRGTVRSIQSHGLSITRLIEEEALKEKTAEIQVQLPPEMATFIMNEKRNFIRDIEKRHGVSVVVIANPYMH
PPQYTITRLKEDNVGKSKKPSYSLIQQPEVHVPSSDKEPVSHDEPAVKSFAEHRVIKHPQTSFIKRLWTSLFGGHSVAHT
VTQEPGKKHHRAQNQGKHPSSKSSGGDKRQQGSNANRRRRSGGGQQRSSSGNTNAAASTHQQRKKSNPNQQSHGSQQSNR
SIPAKNEGNKKREAILAKEQEEK

Sequences:

>Translated_663_residues
MEKMLINAMQTEEIRVALIKNNQLFDLDIECPGEIKKKGNIYKAIVTRREPSLDAVFVEYGSKRQGFLPLKEIAPEYLSK
NPEEFGDEKPPITSLIREGQELLIQVDKEERGNKGAALTTFITLAGCYLVLMPNNPHSGGISRRIEGDERDELRETLNAL
TLPEDMGLIIRTAGVGKSLEELQDDLNMLCNQWQSIKEAYNSELAPCLIHQEGDVIIRSIRDNLRKSISEIIIDDQISYI
KAKQYIERVKPEFLPNLKLYNSSIPLFNFYQIESQIETAYQRQVMLPSGGALVIDRTEALVSIDINSAKATGGADIEATA
LNTNLEAADEIARQLRLRDLGGLVVIDFIDMSSSKNQREVENRLKEALQADRARIQVGRISRFGLLEMSRQRLRLSLGET
AQEICPRCEGRGTVRSIQSHGLSITRLIEEEALKEKTAEIQVQLPPEMATFIMNEKRNFIRDIEKRHGVSVVVIANPYMH
PPQYTITRLKEDNVGKSKKPSYSLIQQPEVHVPSSDKEPVSHDEPAVKSFAEHRVIKHPQTSFIKRLWTSLFGGHSVAHT
VTQEPGKKHHRAQNQGKHPSSKSSGGDKRQQGSNANRRRRSGGGQQRSSSGNTNAAASTHQQRKKSNPNQQSHGSQQSNR
SIPAKNEGNKKREAILAKEQEEK
>Mature_663_residues
MEKMLINAMQTEEIRVALIKNNQLFDLDIECPGEIKKKGNIYKAIVTRREPSLDAVFVEYGSKRQGFLPLKEIAPEYLSK
NPEEFGDEKPPITSLIREGQELLIQVDKEERGNKGAALTTFITLAGCYLVLMPNNPHSGGISRRIEGDERDELRETLNAL
TLPEDMGLIIRTAGVGKSLEELQDDLNMLCNQWQSIKEAYNSELAPCLIHQEGDVIIRSIRDNLRKSISEIIIDDQISYI
KAKQYIERVKPEFLPNLKLYNSSIPLFNFYQIESQIETAYQRQVMLPSGGALVIDRTEALVSIDINSAKATGGADIEATA
LNTNLEAADEIARQLRLRDLGGLVVIDFIDMSSSKNQREVENRLKEALQADRARIQVGRISRFGLLEMSRQRLRLSLGET
AQEICPRCEGRGTVRSIQSHGLSITRLIEEEALKEKTAEIQVQLPPEMATFIMNEKRNFIRDIEKRHGVSVVVIANPYMH
PPQYTITRLKEDNVGKSKKPSYSLIQQPEVHVPSSDKEPVSHDEPAVKSFAEHRVIKHPQTSFIKRLWTSLFGGHSVAHT
VTQEPGKKHHRAQNQGKHPSSKSSGGDKRQQGSNANRRRRSGGGQQRSSSGNTNAAASTHQQRKKSNPNQQSHGSQQSNR
SIPAKNEGNKKREAILAKEQEEK

Specific function: Matures 5S rRNA from its precursors from all the rRNA genes. Major endoribonuclease participating in mRNA turnover. It initiates the decay of RNAs by cutting them internally near their 5'-end. It is able to remove poly(A) tails by an endonucleolytic proce

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1787325, Length=540, Percent_Identity=58.1481481481482, Blast_Score=613, Evalue=1e-177,
Organism=Escherichia coli, GI87082235, Length=489, Percent_Identity=34.560327198364, Blast_Score=239, Evalue=4e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR021968
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF12111 PNPase_C; PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 74503; Mature: 74503

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKMLINAMQTEEIRVALIKNNQLFDLDIECPGEIKKKGNIYKAIVTRREPSLDAVFVEY
CCCHHHHHHCCCCEEEEEEECCEEEEEECCCCCHHHHCCCEEEEHHCCCCCCCCEEEEEC
GSKRQGFLPLKEIAPEYLSKNPEEFGDEKPPITSLIREGQELLIQVDKEERGNKGAALTT
CCCCCCCCCHHHHCHHHHCCCCHHHCCCCCCHHHHHHCCHHHEEEECCCCCCCCCCHHHH
FITLAGCYLVLMPNNPHSGGISRRIEGDERDELRETLNALTLPEDMGLIIRTAGVGKSLE
HHHHHCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCHHH
ELQDDLNMLCNQWQSIKEAYNSELAPCLIHQEGDVIIRSIRDNLRKSISEIIIDDQISYI
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCHHHHH
KAKQYIERVKPEFLPNLKLYNSSIPLFNFYQIESQIETAYQRQVMLPSGGALVIDRTEAL
HHHHHHHHHCHHHCCCCEEECCCCCEEEHHHHHHHHHHHHHHEEECCCCCEEEEECCCEE
VSIDINSAKATGGADIEATALNTNLEAADEIARQLRLRDLGGLVVIDFIDMSSSKNQREV
EEEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHH
ENRLKEALQADRARIQVGRISRFGLLEMSRQRLRLSLGETAQEICPRCEGRGTVRSIQSH
HHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHC
GLSITRLIEEEALKEKTAEIQVQLPPEMATFIMNEKRNFIRDIEKRHGVSVVVIANPYMH
CCHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCC
PPQYTITRLKEDNVGKSKKPSYSLIQQPEVHVPSSDKEPVSHDEPAVKSFAEHRVIKHPQ
CCCHHHHEECCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCH
TSFIKRLWTSLFGGHSVAHTVTQEPGKKHHRAQNQGKHPSSKSSGGDKRQQGSNANRRRR
HHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCHHHCCCCCHHHHH
SGGGQQRSSSGNTNAAASTHQQRKKSNPNQQSHGSQQSNRSIPAKNEGNKKREAILAKEQ
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHCCCCHHCCCCCCCCCCCHHHHHHHHHHC
EEK
CCC
>Mature Secondary Structure
MEKMLINAMQTEEIRVALIKNNQLFDLDIECPGEIKKKGNIYKAIVTRREPSLDAVFVEY
CCCHHHHHHCCCCEEEEEEECCEEEEEECCCCCHHHHCCCEEEEHHCCCCCCCCEEEEEC
GSKRQGFLPLKEIAPEYLSKNPEEFGDEKPPITSLIREGQELLIQVDKEERGNKGAALTT
CCCCCCCCCHHHHCHHHHCCCCHHHCCCCCCHHHHHHCCHHHEEEECCCCCCCCCCHHHH
FITLAGCYLVLMPNNPHSGGISRRIEGDERDELRETLNALTLPEDMGLIIRTAGVGKSLE
HHHHHCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCHHH
ELQDDLNMLCNQWQSIKEAYNSELAPCLIHQEGDVIIRSIRDNLRKSISEIIIDDQISYI
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCHHHHH
KAKQYIERVKPEFLPNLKLYNSSIPLFNFYQIESQIETAYQRQVMLPSGGALVIDRTEAL
HHHHHHHHHCHHHCCCCEEECCCCCEEEHHHHHHHHHHHHHHEEECCCCCEEEEECCCEE
VSIDINSAKATGGADIEATALNTNLEAADEIARQLRLRDLGGLVVIDFIDMSSSKNQREV
EEEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHH
ENRLKEALQADRARIQVGRISRFGLLEMSRQRLRLSLGETAQEICPRCEGRGTVRSIQSH
HHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHC
GLSITRLIEEEALKEKTAEIQVQLPPEMATFIMNEKRNFIRDIEKRHGVSVVVIANPYMH
CCHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCC
PPQYTITRLKEDNVGKSKKPSYSLIQQPEVHVPSSDKEPVSHDEPAVKSFAEHRVIKHPQ
CCCHHHHEECCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCH
TSFIKRLWTSLFGGHSVAHTVTQEPGKKHHRAQNQGKHPSSKSSGGDKRQQGSNANRRRR
HHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCHHHCCCCCHHHHH
SGGGQQRSSSGNTNAAASTHQQRKKSNPNQQSHGSQQSNRSIPAKNEGNKKREAILAKEQ
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHCCCCHHCCCCCCCCCCCHHHHHHHHHHC
EEK
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]