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The map label for this gene is gatA [H]

Identifier: 54294624

GI number: 54294624

Start: 1897314

End: 1898765

Strand: Direct

Name: gatA [H]

Synonym: lpl1700

Alternate gene names: 54294624

Gene position: 1897314-1898765 (Clockwise)

Preceding gene: 54294623

Following gene: 54294625

Centisome position: 56.71

GC content: 40.5

Gene sequence:

>1452_bases
ATGGAACATTACTCACTGGCTCAATTATCAAAGGCTCTTCATAATCGAGAATTTTCAAGTGTTGAATTAACACAACACTG
CATTAACAAAATTCAAAGCAATAAAGACTTGAATGCATTCATCAGCCTTGATGAAGACCAGGCTCTTAAAGAAGCGCAAT
CGGCTGATTTGGTACTAAAAAATGGAGAGGGAAAGCCACTGACTGGGATCCCCATGGCTCTCAAGGATTTGTTTTGTACA
AAGCGATTAAATACAACTTGTGCTTCAAAAATGCTGGCTAATTTTCAAGCCCCCTATGATGCCACTATCGTCACAAAATT
TAAACAAAATGGCGCAATAATTATTGGAAAAACCAATATGGATGAGTTTGCCATGGGATCCTCTAATGAAAACAGTTACT
TCGGTTCGGTAAAAAACCCGTGGGATAGAGAAAGAGTGCCAGGTGGATCATCAGGCGGTTCTGCAGCAGCGGTTGCTGGA
AGTCTGGTACCTTTTGCAATTGGCTCTGACACCGGTGGATCAATTCGTCAGCCAGCAGCCTTTTGCGGAATTAGCGGGAT
AAAACCCACCTATGGATTAGTCTCTCGATACGGAATGGTTGCCTTTGCCTCAAGCCTTGATCAAGCGGGACCATTTGCGA
AGTCAGCTGAAGATCTGGCCATGATACTTCACTGTATTGCGGGGTTTGATAGTAAAGACTCCACGTCAGCTGATAGAGTT
ATACCTGATTATTCAACAGAAATTAAAAAACCAGTAGACAAAATACGCATAGGACTTCCTTCTTGCTTTTTTCAACCTCA
AGTCGAAAAAGGTATCCAGGATGCCATACATGATGCTGTGAAATTATTTGAGAATCTAGGGGCTGAAATAATTGAAATCG
ACTTGAAACTTCAGCCACTTTGGGTACCATGTTACTATGTTATAGCTTGCGCAGAAGCTTCATCAAATCTTTCTCGTTAT
GATGGCATAAGATTTGGCCATCGAAGCAAATCCGCATCAACCTTAATTGAATTAATCACTAACTCTCGAAGTGAAGGTTT
TGGTAACGAGGTAAAACGTCGAATTCTAACTGGAACTCATGTGCTCTCATCCGGTTTTTTTGATGCCTATTATTTGCATG
CACAAAAAGTGAGGCGTTTGATTCGAGATGAGTTAATTACAATTTTAAATTCAGTAGATGTTATCCTTGGGCCAACAACT
CCTACCACCGCTTTTAAATTGGGTGAAAAAATAGATGATCCCATACAAAATTATTTGGCAGATGTATTTACTGTAGCAGC
CAATCTTGCAGGCCTACCAGCTATTTCTATACCAACTGGTTTTGAAAATAAACTACCAATTGGTTTGCAATTAATGGGCA
AGCACTTTAGTGAAAATCGCTTGCTTGCAATCGCACATCATTATCAGCAACACACCAACTGGCATTTAGCCAATCCTAAC
AAGCAAGGGTGA

Upstream 100 bases:

>100_bases
TAACTGAAGCGGAATGTCTTGCTGAATTAGAAGCCATGGCGCCATTATTTGAGGATAACTTATATTTGGTTCCCAAGGTG
ATATCATCGGATAAATACTA

Downstream 100 bases:

>100_bases
TATATGGAATGGGATACCGTCATTGGTCTTGAGGTACATGCTCAATTAAAAACCAAATCAAAATTATTTTCAGGAGCATC
TACCGCTTTTGGTGCCACCC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MEHYSLAQLSKALHNREFSSVELTQHCINKIQSNKDLNAFISLDEDQALKEAQSADLVLKNGEGKPLTGIPMALKDLFCT
KRLNTTCASKMLANFQAPYDATIVTKFKQNGAIIIGKTNMDEFAMGSSNENSYFGSVKNPWDRERVPGGSSGGSAAAVAG
SLVPFAIGSDTGGSIRQPAAFCGISGIKPTYGLVSRYGMVAFASSLDQAGPFAKSAEDLAMILHCIAGFDSKDSTSADRV
IPDYSTEIKKPVDKIRIGLPSCFFQPQVEKGIQDAIHDAVKLFENLGAEIIEIDLKLQPLWVPCYYVIACAEASSNLSRY
DGIRFGHRSKSASTLIELITNSRSEGFGNEVKRRILTGTHVLSSGFFDAYYLHAQKVRRLIRDELITILNSVDVILGPTT
PTTAFKLGEKIDDPIQNYLADVFTVAANLAGLPAISIPTGFENKLPIGLQLMGKHFSENRLLAIAHHYQQHTNWHLANPN
KQG

Sequences:

>Translated_483_residues
MEHYSLAQLSKALHNREFSSVELTQHCINKIQSNKDLNAFISLDEDQALKEAQSADLVLKNGEGKPLTGIPMALKDLFCT
KRLNTTCASKMLANFQAPYDATIVTKFKQNGAIIIGKTNMDEFAMGSSNENSYFGSVKNPWDRERVPGGSSGGSAAAVAG
SLVPFAIGSDTGGSIRQPAAFCGISGIKPTYGLVSRYGMVAFASSLDQAGPFAKSAEDLAMILHCIAGFDSKDSTSADRV
IPDYSTEIKKPVDKIRIGLPSCFFQPQVEKGIQDAIHDAVKLFENLGAEIIEIDLKLQPLWVPCYYVIACAEASSNLSRY
DGIRFGHRSKSASTLIELITNSRSEGFGNEVKRRILTGTHVLSSGFFDAYYLHAQKVRRLIRDELITILNSVDVILGPTT
PTTAFKLGEKIDDPIQNYLADVFTVAANLAGLPAISIPTGFENKLPIGLQLMGKHFSENRLLAIAHHYQQHTNWHLANPN
KQG
>Mature_483_residues
MEHYSLAQLSKALHNREFSSVELTQHCINKIQSNKDLNAFISLDEDQALKEAQSADLVLKNGEGKPLTGIPMALKDLFCT
KRLNTTCASKMLANFQAPYDATIVTKFKQNGAIIIGKTNMDEFAMGSSNENSYFGSVKNPWDRERVPGGSSGGSAAAVAG
SLVPFAIGSDTGGSIRQPAAFCGISGIKPTYGLVSRYGMVAFASSLDQAGPFAKSAEDLAMILHCIAGFDSKDSTSADRV
IPDYSTEIKKPVDKIRIGLPSCFFQPQVEKGIQDAIHDAVKLFENLGAEIIEIDLKLQPLWVPCYYVIACAEASSNLSRY
DGIRFGHRSKSASTLIELITNSRSEGFGNEVKRRILTGTHVLSSGFFDAYYLHAQKVRRLIRDELITILNSVDVILGPTT
PTTAFKLGEKIDDPIQNYLADVFTVAANLAGLPAISIPTGFENKLPIGLQLMGKHFSENRLLAIAHHYQQHTNWHLANPN
KQG

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=503, Percent_Identity=38.3697813121272, Blast_Score=345, Evalue=5e-95,
Organism=Homo sapiens, GI195972892, Length=491, Percent_Identity=27.9022403258656, Blast_Score=149, Evalue=7e-36,
Organism=Homo sapiens, GI166795287, Length=212, Percent_Identity=30.6603773584906, Blast_Score=74, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17543272, Length=451, Percent_Identity=40.1330376940133, Blast_Score=292, Evalue=3e-79,
Organism=Caenorhabditis elegans, GI17537465, Length=507, Percent_Identity=25.8382642998028, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17556264, Length=302, Percent_Identity=28.1456953642384, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI71990152, Length=321, Percent_Identity=25.8566978193146, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17538252, Length=320, Percent_Identity=28.4375, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17556276, Length=187, Percent_Identity=32.0855614973262, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17556278, Length=187, Percent_Identity=32.0855614973262, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17538254, Length=324, Percent_Identity=24.6913580246914, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=426, Percent_Identity=36.3849765258216, Blast_Score=246, Evalue=6e-66,
Organism=Saccharomyces cerevisiae, GI6319685, Length=450, Percent_Identity=32.2222222222222, Blast_Score=144, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6320448, Length=531, Percent_Identity=22.0338983050847, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24648113, Length=486, Percent_Identity=39.0946502057613, Blast_Score=307, Evalue=8e-84,
Organism=Drosophila melanogaster, GI24644968, Length=494, Percent_Identity=27.9352226720648, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24652985, Length=504, Percent_Identity=27.7777777777778, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI19922090, Length=504, Percent_Identity=27.7777777777778, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24652981, Length=504, Percent_Identity=27.7777777777778, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24652983, Length=504, Percent_Identity=27.7777777777778, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI161078093, Length=502, Percent_Identity=29.2828685258964, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI45550774, Length=515, Percent_Identity=26.2135922330097, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24648435, Length=515, Percent_Identity=26.2135922330097, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24648437, Length=515, Percent_Identity=26.2135922330097, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI21356731, Length=236, Percent_Identity=32.6271186440678, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24648441, Length=451, Percent_Identity=26.1640798226164, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24648439, Length=451, Percent_Identity=26.1640798226164, Blast_Score=101, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 52620; Mature: 52620

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHYSLAQLSKALHNREFSSVELTQHCINKIQSNKDLNAFISLDEDQALKEAQSADLVLK
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHCCCCEEEE
NGEGKPLTGIPMALKDLFCTKRLNTTCASKMLANFQAPYDATIVTKFKQNGAIIIGKTNM
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEECCCC
DEFAMGSSNENSYFGSVKNPWDRERVPGGSSGGSAAAVAGSLVPFAIGSDTGGSIRQPAA
HHHHCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCHHHHHHCCCEEEECCCCCCCCCCCHH
FCGISGIKPTYGLVSRYGMVAFASSLDQAGPFAKSAEDLAMILHCIAGFDSKDSTSADRV
HHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
IPDYSTEIKKPVDKIRIGLPSCFFQPQVEKGIQDAIHDAVKLFENLGAEIIEIDLKLQPL
CCCCHHHHHCCHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEE
WVPCYYVIACAEASSNLSRYDGIRFGHRSKSASTLIELITNSRSEGFGNEVKRRILTGTH
EEHHHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
VLSSGFFDAYYLHAQKVRRLIRDELITILNSVDVILGPTTPTTAFKLGEKIDDPIQNYLA
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHH
DVFTVAANLAGLPAISIPTGFENKLPIGLQLMGKHFSENRLLAIAHHYQQHTNWHLANPN
HHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHCCCCCCCCEEEHHHHHHHCCCEEECCCC
KQG
CCC
>Mature Secondary Structure
MEHYSLAQLSKALHNREFSSVELTQHCINKIQSNKDLNAFISLDEDQALKEAQSADLVLK
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHCCCCEEEE
NGEGKPLTGIPMALKDLFCTKRLNTTCASKMLANFQAPYDATIVTKFKQNGAIIIGKTNM
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEECCCC
DEFAMGSSNENSYFGSVKNPWDRERVPGGSSGGSAAAVAGSLVPFAIGSDTGGSIRQPAA
HHHHCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCHHHHHHCCCEEEECCCCCCCCCCCHH
FCGISGIKPTYGLVSRYGMVAFASSLDQAGPFAKSAEDLAMILHCIAGFDSKDSTSADRV
HHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
IPDYSTEIKKPVDKIRIGLPSCFFQPQVEKGIQDAIHDAVKLFENLGAEIIEIDLKLQPL
CCCCHHHHHCCHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEE
WVPCYYVIACAEASSNLSRYDGIRFGHRSKSASTLIELITNSRSEGFGNEVKRRILTGTH
EEHHHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
VLSSGFFDAYYLHAQKVRRLIRDELITILNSVDVILGPTTPTTAFKLGEKIDDPIQNYLA
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHH
DVFTVAANLAGLPAISIPTGFENKLPIGLQLMGKHFSENRLLAIAHHYQQHTNWHLANPN
HHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHCCCCCCCCEEEHHHHHHHCCCEEECCCC
KQG
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA