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The map label for this gene is gatB [H]

Identifier: 54294625

GI number: 54294625

Start: 1898769

End: 1900202

Strand: Direct

Name: gatB [H]

Synonym: lpl1701

Alternate gene names: 54294625

Gene position: 1898769-1900202 (Clockwise)

Preceding gene: 54294624

Following gene: 54294626

Centisome position: 56.75

GC content: 38.08

Gene sequence:

>1434_bases
ATGGAATGGGATACCGTCATTGGTCTTGAGGTACATGCTCAATTAAAAACCAAATCAAAATTATTTTCAGGAGCATCTAC
CGCTTTTGGTGCCACCCCTAACTCGCAAACAAGTTTTATCGATGCTGGTTTGCCTGGTGTTCTCCCTGTGTTAAATGAAC
AGGCCATCATAATGGCTATCCAATTTGGTTTGGCAATTCATGGAACCATTAATGATTTGTCTGTTTTTGAACGCAAAAAT
TATTTTTATCCTGACTTACCCAAAGGCTATCAAATCAGTCAGTATCAAAAACCCATTGTTACTAATGGCTATTTAAATAT
ACAGCTTGGTAATAATCTGGAAAAAACAGTCCATATTGCACGCGCTCATTTAGAGGAAGATGCTGGCAAATCCCTGCACG
ATGCCCATACTGATTACACCGGGATCGACCTCAACAGAGCAGGTACCCCACTATTGGAAATAGTCACAACCCCTTGCCTG
TACTCGGCTGAAGAGGCGATTAATTATTTAAAAACGCTGCATCAATTAGTGAGATTCCTTGGAATATGCGATGGCAATAT
GCAAGAAGGTTCTTTTCGGTGTGACGTCAATCTGTCTATAAAACCTAAAGGCAGCTCAGTTTTAGGCACACGAACAGAAC
TTAAAAATCTGAATTCATTTCGTTTTATTGAAAAAGCCATTGCTTTTGAACAGGCAAGACACCAAGATATATTGGAAAGC
GGTCTGTCAGTCATCCAGGAGACTCGCCTTTACAACCCTGACAATAACACAACTCAGGCGATGAGAGGCAAGGAAAATGA
AAATGATTACCGTTATTTTCCTGATCCTGATTTATTACCAATTCATATTGACAAAGAACAAATTGAAGAGATTAAAAATA
ACCTGCCGGACTTGCCTGAAGCAATTTCCAAAGAATTAAAAAACACTCCGTCCCTAAATGATGAAGACATTAATTTCATA
TTATCGTCACCGGACACTTATCAATATTATAAAAAAATTAAATCCCTTTGTTCGGCTGCTGATAAAACAATTATCAACTG
GTTAAAAGGCCAATATGCAGCCTTTCTTAATGAACACACCCTAACCTTTGAAACACCACCAATTTCAGCTAAAACCATGG
CTGCTTTCCTTAGCAAAATTCATGAGAAAAAAATCTCTTCCAGTATTGCTAAAAATATTTTCAGTATGCTTTGTGCAGGC
GAAGAAGATATCGATGCGATTATTGAACGCGAAGGCTACCAACAACAGAATGACAACTCGGCACTGGAAGAAATAGTTGA
GCAAATAATCAAACAATATCCCGAGCAAGTTACAGAATACAAAGCCGGTAAAGAGAAATTATTAGCTTTTTTTATCGGAC
AAGCGATGAAACAAACCAAAGGGAAAGCAAATCCCGAGCAAATCAATCTGTTATTAAAAAAACATCTTGGATGA

Upstream 100 bases:

>100_bases
GGGCAAGCACTTTAGTGAAAATCGCTTGCTTGCAATCGCACATCATTATCAGCAACACACCAACTGGCATTTAGCCAATC
CTAACAAGCAAGGGTGATAT

Downstream 100 bases:

>100_bases
TGCAATATTTTTATAATTCACAATGCTCATTTATGGCTTTGTTGATTGATTGCCAGCTATTATCAGCTTTATGTCAGAAG
GTATAATGCAAGCATTCAAT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MEWDTVIGLEVHAQLKTKSKLFSGASTAFGATPNSQTSFIDAGLPGVLPVLNEQAIIMAIQFGLAIHGTINDLSVFERKN
YFYPDLPKGYQISQYQKPIVTNGYLNIQLGNNLEKTVHIARAHLEEDAGKSLHDAHTDYTGIDLNRAGTPLLEIVTTPCL
YSAEEAINYLKTLHQLVRFLGICDGNMQEGSFRCDVNLSIKPKGSSVLGTRTELKNLNSFRFIEKAIAFEQARHQDILES
GLSVIQETRLYNPDNNTTQAMRGKENENDYRYFPDPDLLPIHIDKEQIEEIKNNLPDLPEAISKELKNTPSLNDEDINFI
LSSPDTYQYYKKIKSLCSAADKTIINWLKGQYAAFLNEHTLTFETPPISAKTMAAFLSKIHEKKISSSIAKNIFSMLCAG
EEDIDAIIEREGYQQQNDNSALEEIVEQIIKQYPEQVTEYKAGKEKLLAFFIGQAMKQTKGKANPEQINLLLKKHLG

Sequences:

>Translated_477_residues
MEWDTVIGLEVHAQLKTKSKLFSGASTAFGATPNSQTSFIDAGLPGVLPVLNEQAIIMAIQFGLAIHGTINDLSVFERKN
YFYPDLPKGYQISQYQKPIVTNGYLNIQLGNNLEKTVHIARAHLEEDAGKSLHDAHTDYTGIDLNRAGTPLLEIVTTPCL
YSAEEAINYLKTLHQLVRFLGICDGNMQEGSFRCDVNLSIKPKGSSVLGTRTELKNLNSFRFIEKAIAFEQARHQDILES
GLSVIQETRLYNPDNNTTQAMRGKENENDYRYFPDPDLLPIHIDKEQIEEIKNNLPDLPEAISKELKNTPSLNDEDINFI
LSSPDTYQYYKKIKSLCSAADKTIINWLKGQYAAFLNEHTLTFETPPISAKTMAAFLSKIHEKKISSSIAKNIFSMLCAG
EEDIDAIIEREGYQQQNDNSALEEIVEQIIKQYPEQVTEYKAGKEKLLAFFIGQAMKQTKGKANPEQINLLLKKHLG
>Mature_477_residues
MEWDTVIGLEVHAQLKTKSKLFSGASTAFGATPNSQTSFIDAGLPGVLPVLNEQAIIMAIQFGLAIHGTINDLSVFERKN
YFYPDLPKGYQISQYQKPIVTNGYLNIQLGNNLEKTVHIARAHLEEDAGKSLHDAHTDYTGIDLNRAGTPLLEIVTTPCL
YSAEEAINYLKTLHQLVRFLGICDGNMQEGSFRCDVNLSIKPKGSSVLGTRTELKNLNSFRFIEKAIAFEQARHQDILES
GLSVIQETRLYNPDNNTTQAMRGKENENDYRYFPDPDLLPIHIDKEQIEEIKNNLPDLPEAISKELKNTPSLNDEDINFI
LSSPDTYQYYKKIKSLCSAADKTIINWLKGQYAAFLNEHTLTFETPPISAKTMAAFLSKIHEKKISSSIAKNIFSMLCAG
EEDIDAIIEREGYQQQNDNSALEEIVEQIIKQYPEQVTEYKAGKEKLLAFFIGQAMKQTKGKANPEQINLLLKKHLG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=495, Percent_Identity=36.5656565656566, Blast_Score=313, Evalue=3e-85,
Organism=Caenorhabditis elegans, GI115534094, Length=336, Percent_Identity=37.5, Blast_Score=219, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6319391, Length=510, Percent_Identity=32.5490196078431, Blast_Score=219, Evalue=8e-58,
Organism=Drosophila melanogaster, GI45550808, Length=491, Percent_Identity=35.030549898167, Blast_Score=294, Evalue=1e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: NA

Molecular weight: Translated: 53580; Mature: 53580

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEWDTVIGLEVHAQLKTKSKLFSGASTAFGATPNSQTSFIDAGLPGVLPVLNEQAIIMAI
CCCCEEEEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCHHHHHCCCCEEEEE
QFGLAIHGTINDLSVFERKNYFYPDLPKGYQISQYQKPIVTNGYLNIQLGNNLEKTVHIA
ECCEEEECCCHHHHHHHHCCCCCCCCCCCCCCHHHCCCCEECCEEEEEECCCHHHHHHHH
RAHLEEDAGKSLHDAHTDYTGIDLNRAGTPLLEIVTTPCLYSAEEAINYLKTLHQLVRFL
HHHHHHHCCCCHHHCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
GICDGNMQEGSFRCDVNLSIKPKGSSVLGTRTELKNLNSFRFIEKAIAFEQARHQDILES
HCCCCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
GLSVIQETRLYNPDNNTTQAMRGKENENDYRYFPDPDLLPIHIDKEQIEEIKNNLPDLPE
HHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHCCCCHHH
AISKELKNTPSLNDEDINFILSSPDTYQYYKKIKSLCSAADKTIINWLKGQYAAFLNEHT
HHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
LTFETPPISAKTMAAFLSKIHEKKISSSIAKNIFSMLCAGEEDIDAIIEREGYQQQNDNS
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCH
ALEEIVEQIIKQYPEQVTEYKAGKEKLLAFFIGQAMKQTKGKANPEQINLLLKKHLG
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MEWDTVIGLEVHAQLKTKSKLFSGASTAFGATPNSQTSFIDAGLPGVLPVLNEQAIIMAI
CCCCEEEEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCHHHHHCCCCEEEEE
QFGLAIHGTINDLSVFERKNYFYPDLPKGYQISQYQKPIVTNGYLNIQLGNNLEKTVHIA
ECCEEEECCCHHHHHHHHCCCCCCCCCCCCCCHHHCCCCEECCEEEEEECCCHHHHHHHH
RAHLEEDAGKSLHDAHTDYTGIDLNRAGTPLLEIVTTPCLYSAEEAINYLKTLHQLVRFL
HHHHHHHCCCCHHHCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
GICDGNMQEGSFRCDVNLSIKPKGSSVLGTRTELKNLNSFRFIEKAIAFEQARHQDILES
HCCCCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
GLSVIQETRLYNPDNNTTQAMRGKENENDYRYFPDPDLLPIHIDKEQIEEIKNNLPDLPE
HHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHCCCCHHH
AISKELKNTPSLNDEDINFILSSPDTYQYYKKIKSLCSAADKTIINWLKGQYAAFLNEHT
HHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
LTFETPPISAKTMAAFLSKIHEKKISSSIAKNIFSMLCAGEEDIDAIIEREGYQQQNDNS
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCH
ALEEIVEQIIKQYPEQVTEYKAGKEKLLAFFIGQAMKQTKGKANPEQINLLLKKHLG
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA