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The map label for this gene is trpE [H]

Identifier: 54294622

GI number: 54294622

Start: 1894594

End: 1896750

Strand: Reverse

Name: trpE [H]

Synonym: lpl1698

Alternate gene names: 54294622

Gene position: 1896750-1894594 (Counterclockwise)

Preceding gene: 54294630

Following gene: 54294621

Centisome position: 56.69

GC content: 38.2

Gene sequence:

>2157_bases
ATGGTATTCATGTTACAGCAATATAAAACTCTTGGTGGTGTTCATGTTGAGTTTTCTCAACAAGAGTTGGATTATCAACA
AGGCATAGATTTTTTGCTTGAGAGCCTTGACTCTCAACGTGGCGCAGTATTTGCTTCGAGCTTTGAATATCCGGGACGCT
ATACTTGTTGGGACATGGGTTTTTATAATCCCCCATTAGTTATGATCTGTCAAAGCAATACCATCTGTTTTGAGGCATTA
AATCAACGAGGCGAGATATTATTATCTTTTCTCTATCCGTTATTAAAAACAACAGATCTCTGGGAGGTAATTAGCCAAAG
GACCTCATTTTGTCAGTTAAAAATTAAATCTTCAGACAAAGTATTTAGTGAGGAGGAGCGGAGCCATCAACCATCAGTAT
TCAGCGCAATTCGCCTCTTATTGAATTTTTTTAAAACAGAAGAAGATTCATACTTGGGTTTATATGGTGCTTTTGGGTTT
GATCTGATTTATCAATTTGAACAGTTGGAGCAGTGTAAACCAAGAAATGAAACACAGAGAGATATGGTTCTCTATCTACC
TGATGAAATTTATATAGTGAATCATCGTAAGGAAGAAGCCTTTGTTAGACGCTATGATTTTCAATATCAGGGGCGATCAA
CTCAATCTTTATCCAGAGAAGGAAAGTATTCACCATATCAACCTATCCACAAACCGGAAAAAAACTGTGATCACGAGCCA
GGTGAATATGCTGAAGTGGTTAAAAAAGCTAAAGAAAAATTTTCCTGTGGTGATTTATTTGAAGTGGTTCCTAGCCAAAC
TTTCTATACTCACTTTGCTGAAAAGCCGTCAATATTATTTCGCCAAATGCGAGAAATAAATCCATCGCCATATGGATTTT
TTGTTAATTTGGGTGACGAAGAATATCTGGTAGGTGCTTCACCTGAAATGTATGTTCGTGTTCAAGGTAAAAGAGTAGAA
ACTTGCCCTATCTCAGGCACTATCAAACGGGGAGCAGATGCTATTGAGGATGCTCACAATATTCAGACACTTCTAGATTC
AGAGAAAGAAGAATCAGAACTAACCATGTGTACCGATGTGGATAGAAATGATAAGTCAAGGATATGCGAAGCAGGGAGTG
TGAAAGTCATTGGGCGTCGCCAAATTGAAATGTATTCTCGATTGATTCATACAGTTGATCATGTTGAGGGTGTTTTAAGA
GATGGATTTGATGCAGTAGATGCTTTTTTAACTCATATGTGGGTTGTTACTGTGACTGGTGCGCCTAAAATTTGGGCTAT
GAACTTCATTGAACAACATGAGAAATCCCCGCGTAAATGGTATGCAGGGGCCGTAGGATGGTTTGGTTTTGATGGAAATT
TAAATACTGGATTGGTCTTAAGAACAGTGCGAATAGAAAAGGGAACTGCAGAAATTAGAGTAGGCGCAACGTTACTTTAT
GACTCTATTCCTGAAGCTGAGGAAGAGGAGACGAGATTAAAGGCTTCTGCATTTTTAGACATTTTACAAAAACCATGGCA
AACAGCAAAAAAGAAGACAGAGAATATCCCCCTGGTAGGGGAAAATAAAAAAGTTTTACTTATCGATCATCAAGATTCCT
TTGTTCATACTTTGGCAAACTATTTAAGACAAACGGGAGCTGCGGTTACAACAGTTCGTTATGACAAGGCGATTGGCTAT
TTACAAAATAATCATTTTGATTTGATCGTATTATCACCTGGCCCTGGAAAACCGGTTGATTTTAAGCTTTCAGAAACAAT
TGATATGATACTCTCAAAGAACATACCAATATTCGGTGTTTGTCTGGGGCTGCAAGGAATTGTTGAATATTTTGGAGGGG
TTTTGGATGTTCTTGATTACCCTATGCATGGAAAATCTTCAGTAATCAGGGTATTGGATAACAAGGGGTTGTTCTCCGGT
TTAGGTGATTGTTTTAAAGCGGGAAGATACCATTCACTCTATGCCAGAGCGGAGGCTTTACCCAATGAATTATTAGTTAC
CGCTGTAAGTGAAGATGATGTGATTATGGCAGTTTCTCATAAGCATTTACCTGTGCATGCGGTTCAATTTCATCCAGAGA
CAATTTTGTCTTTATCGGATCAAGTGGGTTTAAGAATAATCATCAATTTGATGAACATGGTGAAGGACAATGGGTAA

Upstream 100 bases:

>100_bases
TGAAGACATAAAAAAGCAATGATATTGAAATGTTACAAGATTCTTGCAGAACAGTGGTTTTACAGTAGAATGAATATTTG
TACGAATTAAATGATAGAGA

Downstream 100 bases:

>100_bases
AAAAATACTGATTTTATATTTTGTATCAGTTCTATTAGGGATATTAACAGGCGCAATTGCTTCATTATTGCAACTTACTA
TTCAACAGATGGATCATCTG

Product: anthranilate synthase

Products: NA

Alternate protein names: Glutamine amidotransferase [H]

Number of amino acids: Translated: 718; Mature: 718

Protein sequence:

>718_residues
MVFMLQQYKTLGGVHVEFSQQELDYQQGIDFLLESLDSQRGAVFASSFEYPGRYTCWDMGFYNPPLVMICQSNTICFEAL
NQRGEILLSFLYPLLKTTDLWEVISQRTSFCQLKIKSSDKVFSEEERSHQPSVFSAIRLLLNFFKTEEDSYLGLYGAFGF
DLIYQFEQLEQCKPRNETQRDMVLYLPDEIYIVNHRKEEAFVRRYDFQYQGRSTQSLSREGKYSPYQPIHKPEKNCDHEP
GEYAEVVKKAKEKFSCGDLFEVVPSQTFYTHFAEKPSILFRQMREINPSPYGFFVNLGDEEYLVGASPEMYVRVQGKRVE
TCPISGTIKRGADAIEDAHNIQTLLDSEKEESELTMCTDVDRNDKSRICEAGSVKVIGRRQIEMYSRLIHTVDHVEGVLR
DGFDAVDAFLTHMWVVTVTGAPKIWAMNFIEQHEKSPRKWYAGAVGWFGFDGNLNTGLVLRTVRIEKGTAEIRVGATLLY
DSIPEAEEEETRLKASAFLDILQKPWQTAKKKTENIPLVGENKKVLLIDHQDSFVHTLANYLRQTGAAVTTVRYDKAIGY
LQNNHFDLIVLSPGPGKPVDFKLSETIDMILSKNIPIFGVCLGLQGIVEYFGGVLDVLDYPMHGKSSVIRVLDNKGLFSG
LGDCFKAGRYHSLYARAEALPNELLVTAVSEDDVIMAVSHKHLPVHAVQFHPETILSLSDQVGLRIIINLMNMVKDNG

Sequences:

>Translated_718_residues
MVFMLQQYKTLGGVHVEFSQQELDYQQGIDFLLESLDSQRGAVFASSFEYPGRYTCWDMGFYNPPLVMICQSNTICFEAL
NQRGEILLSFLYPLLKTTDLWEVISQRTSFCQLKIKSSDKVFSEEERSHQPSVFSAIRLLLNFFKTEEDSYLGLYGAFGF
DLIYQFEQLEQCKPRNETQRDMVLYLPDEIYIVNHRKEEAFVRRYDFQYQGRSTQSLSREGKYSPYQPIHKPEKNCDHEP
GEYAEVVKKAKEKFSCGDLFEVVPSQTFYTHFAEKPSILFRQMREINPSPYGFFVNLGDEEYLVGASPEMYVRVQGKRVE
TCPISGTIKRGADAIEDAHNIQTLLDSEKEESELTMCTDVDRNDKSRICEAGSVKVIGRRQIEMYSRLIHTVDHVEGVLR
DGFDAVDAFLTHMWVVTVTGAPKIWAMNFIEQHEKSPRKWYAGAVGWFGFDGNLNTGLVLRTVRIEKGTAEIRVGATLLY
DSIPEAEEEETRLKASAFLDILQKPWQTAKKKTENIPLVGENKKVLLIDHQDSFVHTLANYLRQTGAAVTTVRYDKAIGY
LQNNHFDLIVLSPGPGKPVDFKLSETIDMILSKNIPIFGVCLGLQGIVEYFGGVLDVLDYPMHGKSSVIRVLDNKGLFSG
LGDCFKAGRYHSLYARAEALPNELLVTAVSEDDVIMAVSHKHLPVHAVQFHPETILSLSDQVGLRIIINLMNMVKDNG
>Mature_718_residues
MVFMLQQYKTLGGVHVEFSQQELDYQQGIDFLLESLDSQRGAVFASSFEYPGRYTCWDMGFYNPPLVMICQSNTICFEAL
NQRGEILLSFLYPLLKTTDLWEVISQRTSFCQLKIKSSDKVFSEEERSHQPSVFSAIRLLLNFFKTEEDSYLGLYGAFGF
DLIYQFEQLEQCKPRNETQRDMVLYLPDEIYIVNHRKEEAFVRRYDFQYQGRSTQSLSREGKYSPYQPIHKPEKNCDHEP
GEYAEVVKKAKEKFSCGDLFEVVPSQTFYTHFAEKPSILFRQMREINPSPYGFFVNLGDEEYLVGASPEMYVRVQGKRVE
TCPISGTIKRGADAIEDAHNIQTLLDSEKEESELTMCTDVDRNDKSRICEAGSVKVIGRRQIEMYSRLIHTVDHVEGVLR
DGFDAVDAFLTHMWVVTVTGAPKIWAMNFIEQHEKSPRKWYAGAVGWFGFDGNLNTGLVLRTVRIEKGTAEIRVGATLLY
DSIPEAEEEETRLKASAFLDILQKPWQTAKKKTENIPLVGENKKVLLIDHQDSFVHTLANYLRQTGAAVTTVRYDKAIGY
LQNNHFDLIVLSPGPGKPVDFKLSETIDMILSKNIPIFGVCLGLQGIVEYFGGVLDVLDYPMHGKSSVIRVLDNKGLFSG
LGDCFKAGRYHSLYARAEALPNELLVTAVSEDDVIMAVSHKHLPVHAVQFHPETILSLSDQVGLRIIINLMNMVKDNG

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=421, Percent_Identity=32.541567695962, Blast_Score=196, Evalue=4e-51,
Organism=Escherichia coli, GI1788114, Length=352, Percent_Identity=27.5568181818182, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1789760, Length=189, Percent_Identity=36.5079365079365, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1787517, Length=177, Percent_Identity=36.1581920903955, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1786809, Length=304, Percent_Identity=26.6447368421053, Blast_Score=87, Evalue=5e-18,
Organism=Escherichia coli, GI87082077, Length=288, Percent_Identity=23.2638888888889, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6320935, Length=277, Percent_Identity=36.4620938628159, Blast_Score=152, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6322638, Length=191, Percent_Identity=38.7434554973822, Blast_Score=126, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6324361, Length=125, Percent_Identity=33.6, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR006805
- InterPro:   IPR006220
- InterPro:   IPR010112
- InterPro:   IPR001317
- InterPro:   IPR015890
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR006221 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind; PF00117 GATase [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 81712; Mature: 81712

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFMLQQYKTLGGVHVEFSQQELDYQQGIDFLLESLDSQRGAVFASSFEYPGRYTCWDMG
CEEEEEHHHHHCCEEEEECHHHCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEECC
FYNPPLVMICQSNTICFEALNQRGEILLSFLYPLLKTTDLWEVISQRTSFCQLKIKSSDK
CCCCCEEEEECCCCHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEECCCCC
VFSEEERSHQPSVFSAIRLLLNFFKTEEDSYLGLYGAFGFDLIYQFEQLEQCKPRNETQR
CCCHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCC
DMVLYLPDEIYIVNHRKEEAFVRRYDFQYQGRSTQSLSREGKYSPYQPIHKPEKNCDHEP
CEEEEECCEEEEEECCHHHHHHHHHCCEECCCCHHHHHCCCCCCCCCCCCCCCCCCCCCC
GEYAEVVKKAKEKFSCGDLFEVVPSQTFYTHFAEKPSILFRQMREINPSPYGFFVNLGDE
CHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEECCC
EYLVGASPEMYVRVQGKRVETCPISGTIKRGADAIEDAHNIQTLLDSEKEESELTMCTDV
CEEECCCCCEEEEEECCEEEECCCCCCHHHCCHHHHHHHHHHHHHCCCCCCCCEEEEECC
DRNDKSRICEAGSVKVIGRRQIEMYSRLIHTVDHVEGVLRDGFDAVDAFLTHMWVVTVTG
CCCCHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEEEEEECC
APKIWAMNFIEQHEKSPRKWYAGAVGWFGFDGNLNTGLVLRTVRIEKGTAEIRVGATLLY
CCCHHHHHHHHHHCCCCCHHCCCCCEEEECCCCCCCCEEEEEEEEECCCCEEEECEEEEE
DSIPEAEEEETRLKASAFLDILQKPWQTAKKKTENIPLVGENKKVLLIDHQDSFVHTLAN
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCEEEEEECCCHHHHHHHH
YLRQTGAAVTTVRYDKAIGYLQNNHFDLIVLSPGPGKPVDFKLSETIDMILSKNIPIFGV
HHHHCCCEEEEEEHHHHHHHEECCCEEEEEECCCCCCCCCEEHHHHHHHHHHCCCCEEEH
CLGLQGIVEYFGGVLDVLDYPMHGKSSVIRVLDNKGLFSGLGDCFKAGRYHSLYARAEAL
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHC
PNELLVTAVSEDDVIMAVSHKHLPVHAVQFHPETILSLSDQVGLRIIINLMNMVKDNG
CCEEEEEEECCCCEEEEEECCCCCEEEEEECCHHHHHCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVFMLQQYKTLGGVHVEFSQQELDYQQGIDFLLESLDSQRGAVFASSFEYPGRYTCWDMG
CEEEEEHHHHHCCEEEEECHHHCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEECC
FYNPPLVMICQSNTICFEALNQRGEILLSFLYPLLKTTDLWEVISQRTSFCQLKIKSSDK
CCCCCEEEEECCCCHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEECCCCC
VFSEEERSHQPSVFSAIRLLLNFFKTEEDSYLGLYGAFGFDLIYQFEQLEQCKPRNETQR
CCCHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCC
DMVLYLPDEIYIVNHRKEEAFVRRYDFQYQGRSTQSLSREGKYSPYQPIHKPEKNCDHEP
CEEEEECCEEEEEECCHHHHHHHHHCCEECCCCHHHHHCCCCCCCCCCCCCCCCCCCCCC
GEYAEVVKKAKEKFSCGDLFEVVPSQTFYTHFAEKPSILFRQMREINPSPYGFFVNLGDE
CHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEECCC
EYLVGASPEMYVRVQGKRVETCPISGTIKRGADAIEDAHNIQTLLDSEKEESELTMCTDV
CEEECCCCCEEEEEECCEEEECCCCCCHHHCCHHHHHHHHHHHHHCCCCCCCCEEEEECC
DRNDKSRICEAGSVKVIGRRQIEMYSRLIHTVDHVEGVLRDGFDAVDAFLTHMWVVTVTG
CCCCHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEEEEEECC
APKIWAMNFIEQHEKSPRKWYAGAVGWFGFDGNLNTGLVLRTVRIEKGTAEIRVGATLLY
CCCHHHHHHHHHHCCCCCHHCCCCCEEEECCCCCCCCEEEEEEEEECCCCEEEECEEEEE
DSIPEAEEEETRLKASAFLDILQKPWQTAKKKTENIPLVGENKKVLLIDHQDSFVHTLAN
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCEEEEEECCCHHHHHHHH
YLRQTGAAVTTVRYDKAIGYLQNNHFDLIVLSPGPGKPVDFKLSETIDMILSKNIPIFGV
HHHHCCCEEEEEEHHHHHHHEECCCEEEEEECCCCCCCCCEEHHHHHHHHHHCCCCEEEH
CLGLQGIVEYFGGVLDVLDYPMHGKSSVIRVLDNKGLFSGLGDCFKAGRYHSLYARAEAL
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHC
PNELLVTAVSEDDVIMAVSHKHLPVHAVQFHPETILSLSDQVGLRIIINLMNMVKDNG
CCEEEEEEECCCCEEEEEECCCCCEEEEEECCHHHHHCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656657; 11481430 [H]