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The map label for this gene is dacB [C]

Identifier: 54294489

GI number: 54294489

Start: 1735645

End: 1737291

Strand: Reverse

Name: dacB [C]

Synonym: lpl1558

Alternate gene names: 54294489

Gene position: 1737291-1735645 (Counterclockwise)

Preceding gene: 54294494

Following gene: 54294487

Centisome position: 51.93

GC content: 36.79

Gene sequence:

>1647_bases
ATGAAGAGATACTCGAAAAGATTACTGACTTATATTTATTTACTTATGAAAAGCATACCTCTAGTGTTCACGATGCAACA
ACTATCATATAGCACAGATAAAATCCCTATACCAATCAATAAACTACCTGATGACCTAAACAAAGTGATGAATAAATCTA
TTTATAAAAAGGCGTTGTGGGGGTTACGTGTTGTTGATCTTGATACTGGTAATGTATTGCTTAATCTCAACTCAAGTACT
CAGTTTTACATTGGTTCTGTGCGCAAGATATTCAGTGTAGGCGAACTATTGAATGAAAGAGGAGCTGATTATCAATCTGT
TACTACAGTCCATTATGATGGTGTATTACACAATGGTAATCTGAATGGGGATCTGGTACTCGTTGCCTCTGGTGACTTGA
CCATGGGAGGACGAACTTTACCTGATGGACATGTCGCTATCACTGAATACGATCATAACGAGGCAAATTCATTGGGTAAT
GCAATTATTACACAGACTAATCTTTTAGCAGGTTATCAAAATCTCGCTAAACAAGTTTACAAAAGTGGCATTCGCTCTAT
TTCAGGCGACATTGTGATAGACGATAGATTGTTTGAACCATTTAATTTCAGGGGTGAATTTGATGTAACCCCTATTTTCA
TAAACGATGATGTAGTTGATATTGTAATTCTTCCAGGTTTGATAAACGAGAATGCAAATGTATCGTGGCTGCCATATTCA
TCGGCGTTCACCGTTGTCAACCAACTTTTTACAGTTAATCCGCATGAAAAATATAATCTTGAACTTGAGTCAAAAGTACC
TGCCTGCATAGGTAAACCAGGCTGTTCTGGGATCATTAAAGGCCATTTGCCTATCAATTTTATACCGCCACTTACTCACA
GCTACCCATTAGTACAAACTTTTCGAATCACTAAACCATCTAATTATGCCAGAACAATTTTTATCGAGGCACTACAAGAA
GCAGGCATCAATGTTTCATCGATTGATAAAACCAAAGAAAATCCGAAAAAACTACTAAAATCTACTAACTTGTACAATAA
GAATAACAAAATTGCTGCTTTGAAATCATTGCCATTGGGAGAGCATGTAAAATATATTCTCAAAGTTAGTTATAATATCG
GAGCAGATACCAGCCTGGTGTTATACGGACTAACAAAAGGCGCGCGCTCCATGATTGAAAGTCTAAACGTGGAAAAAAAA
ATCCTTGATAAAAAATATGGCATATCCCCATCAGAATATCATTTTATCGATGGCAGCGGCGGCGGCGAAACGAAAGCAAC
CAATACCGCTATAATAAAATGGCTTACTATAATGTCTAAAAGACCAGTATTTAACATATTTTACAATAGTCTGCCTATTC
TTGCAGTAGATGGTTCACTTGGTTCAATTAATAACTTTCAATCAGATCCATCGCTTTCTGGGGCGACAGGTCGAGTGCAT
GCAAAAACTGGAACTTATGTATTGGAAAATGAGTCGGGTATGTTAGTTAAGGGGCGCTCTCTTGCTGGTTACATTATTAG
TAAAAAAAATCACCGACTTGCATTTGCCTTAGTTGTTAATAATATACCTATACAGTCACTAGAAGATTTATTTGCTGTAG
CTCAGGATCAAGGCATAATAGCATCAATTCTATGGCGAGATTATTGA

Upstream 100 bases:

>100_bases
CTAATTTCCCAATAATTTTTTTATCTGTGTTACAACAGTTATTATGACAAATTTGACAAACCTAAAAGAATAAAAACATA
TATCAATGGAAATGCGAATG

Downstream 100 bases:

>100_bases
TTATTAATATAATAATATTTCATTCAACGATTGAAAGATGTTATCGAAATGGAGATAGTTATAAAATGCATATTTGTCTA
ATTTATTCTCCATTCAATAA

Product: hypothetical protein

Products: NA

Alternate protein names: Serine-Type D-Ala-D-Ala Carboxypeptidase; Peptidase; D-Ala-D-Ala Carboxypeptidase

Number of amino acids: Translated: 548; Mature: 548

Protein sequence:

>548_residues
MKRYSKRLLTYIYLLMKSIPLVFTMQQLSYSTDKIPIPINKLPDDLNKVMNKSIYKKALWGLRVVDLDTGNVLLNLNSST
QFYIGSVRKIFSVGELLNERGADYQSVTTVHYDGVLHNGNLNGDLVLVASGDLTMGGRTLPDGHVAITEYDHNEANSLGN
AIITQTNLLAGYQNLAKQVYKSGIRSISGDIVIDDRLFEPFNFRGEFDVTPIFINDDVVDIVILPGLINENANVSWLPYS
SAFTVVNQLFTVNPHEKYNLELESKVPACIGKPGCSGIIKGHLPINFIPPLTHSYPLVQTFRITKPSNYARTIFIEALQE
AGINVSSIDKTKENPKKLLKSTNLYNKNNKIAALKSLPLGEHVKYILKVSYNIGADTSLVLYGLTKGARSMIESLNVEKK
ILDKKYGISPSEYHFIDGSGGGETKATNTAIIKWLTIMSKRPVFNIFYNSLPILAVDGSLGSINNFQSDPSLSGATGRVH
AKTGTYVLENESGMLVKGRSLAGYIISKKNHRLAFALVVNNIPIQSLEDLFAVAQDQGIIASILWRDY

Sequences:

>Translated_548_residues
MKRYSKRLLTYIYLLMKSIPLVFTMQQLSYSTDKIPIPINKLPDDLNKVMNKSIYKKALWGLRVVDLDTGNVLLNLNSST
QFYIGSVRKIFSVGELLNERGADYQSVTTVHYDGVLHNGNLNGDLVLVASGDLTMGGRTLPDGHVAITEYDHNEANSLGN
AIITQTNLLAGYQNLAKQVYKSGIRSISGDIVIDDRLFEPFNFRGEFDVTPIFINDDVVDIVILPGLINENANVSWLPYS
SAFTVVNQLFTVNPHEKYNLELESKVPACIGKPGCSGIIKGHLPINFIPPLTHSYPLVQTFRITKPSNYARTIFIEALQE
AGINVSSIDKTKENPKKLLKSTNLYNKNNKIAALKSLPLGEHVKYILKVSYNIGADTSLVLYGLTKGARSMIESLNVEKK
ILDKKYGISPSEYHFIDGSGGGETKATNTAIIKWLTIMSKRPVFNIFYNSLPILAVDGSLGSINNFQSDPSLSGATGRVH
AKTGTYVLENESGMLVKGRSLAGYIISKKNHRLAFALVVNNIPIQSLEDLFAVAQDQGIIASILWRDY
>Mature_548_residues
MKRYSKRLLTYIYLLMKSIPLVFTMQQLSYSTDKIPIPINKLPDDLNKVMNKSIYKKALWGLRVVDLDTGNVLLNLNSST
QFYIGSVRKIFSVGELLNERGADYQSVTTVHYDGVLHNGNLNGDLVLVASGDLTMGGRTLPDGHVAITEYDHNEANSLGN
AIITQTNLLAGYQNLAKQVYKSGIRSISGDIVIDDRLFEPFNFRGEFDVTPIFINDDVVDIVILPGLINENANVSWLPYS
SAFTVVNQLFTVNPHEKYNLELESKVPACIGKPGCSGIIKGHLPINFIPPLTHSYPLVQTFRITKPSNYARTIFIEALQE
AGINVSSIDKTKENPKKLLKSTNLYNKNNKIAALKSLPLGEHVKYILKVSYNIGADTSLVLYGLTKGARSMIESLNVEKK
ILDKKYGISPSEYHFIDGSGGGETKATNTAIIKWLTIMSKRPVFNIFYNSLPILAVDGSLGSINNFQSDPSLSGATGRVH
AKTGTYVLENESGMLVKGRSLAGYIISKKNHRLAFALVVNNIPIQSLEDLFAVAQDQGIIASILWRDY

Specific function: Not Involved In Transpeptidation But Exclusively Catalyzes A Dd-Carboxypeptidase And Dd-Endopeptidase Reaction. [C]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.4.16.4; 3.4.99.- [C]

Molecular weight: Translated: 60587; Mature: 60587

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: PS00146 BETA_LACTAMASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRYSKRLLTYIYLLMKSIPLVFTMQQLSYSTDKIPIPINKLPDDLNKVMNKSIYKKALW
CCHHHHHHHHHHHHHHHCCCCEEEEHHHCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHH
GLRVVDLDTGNVLLNLNSSTQFYIGSVRKIFSVGELLNERGADYQSVTTVHYDGVLHNGN
CEEEEEECCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCEEECCC
LNGDLVLVASGDLTMGGRTLPDGHVAITEYDHNEANSLGNAIITQTNLLAGYQNLAKQVY
CCCCEEEEECCCEEECCEECCCCCEEEEECCCCHHHHHCCEEEEHHHHHHHHHHHHHHHH
KSGIRSISGDIVIDDRLFEPFNFRGEFDVTPIFINDDVVDIVILPGLINENANVSWLPYS
HHHHHHCCCCEEEECCCCCCCCCCCCCCEEEEEECCCEEEEEEECCEECCCCCEEEEECC
SAFTVVNQLFTVNPHEKYNLELESKVPACIGKPGCSGIIKGHLPINFIPPLTHSYPLVQT
HHHHHHHHHEECCCCCCEEEEECCCCCHHCCCCCCCCEEECCCCEEEECCCCCCCCCEEE
FRITKPSNYARTIFIEALQEAGINVSSIDKTKENPKKLLKSTNLYNKNNKIAALKSLPLG
EEECCCCCCHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCH
EHVKYILKVSYNIGADTSLVLYGLTKGARSMIESLNVEKKILDKKYGISPSEYHFIDGSG
HHEEEEEEEEECCCCCCEEEEEECCHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEECCC
GGETKATNTAIIKWLTIMSKRPVFNIFYNSLPILAVDGSLGSINNFQSDPSLSGATGRVH
CCCCCCCHHHHHHHHHHHCCCCEEEEHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCEEE
AKTGTYVLENESGMLVKGRSLAGYIISKKNHRLAFALVVNNIPIQSLEDLFAVAQDQGII
ECCCCEEEECCCCCEEECCCEEEEEEECCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCE
ASILWRDY
EEHEECCC
>Mature Secondary Structure
MKRYSKRLLTYIYLLMKSIPLVFTMQQLSYSTDKIPIPINKLPDDLNKVMNKSIYKKALW
CCHHHHHHHHHHHHHHHCCCCEEEEHHHCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHH
GLRVVDLDTGNVLLNLNSSTQFYIGSVRKIFSVGELLNERGADYQSVTTVHYDGVLHNGN
CEEEEEECCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCEEECCC
LNGDLVLVASGDLTMGGRTLPDGHVAITEYDHNEANSLGNAIITQTNLLAGYQNLAKQVY
CCCCEEEEECCCEEECCEECCCCCEEEEECCCCHHHHHCCEEEEHHHHHHHHHHHHHHHH
KSGIRSISGDIVIDDRLFEPFNFRGEFDVTPIFINDDVVDIVILPGLINENANVSWLPYS
HHHHHHCCCCEEEECCCCCCCCCCCCCCEEEEEECCCEEEEEEECCEECCCCCEEEEECC
SAFTVVNQLFTVNPHEKYNLELESKVPACIGKPGCSGIIKGHLPINFIPPLTHSYPLVQT
HHHHHHHHHEECCCCCCEEEEECCCCCHHCCCCCCCCEEECCCCEEEECCCCCCCCCEEE
FRITKPSNYARTIFIEALQEAGINVSSIDKTKENPKKLLKSTNLYNKNNKIAALKSLPLG
EEECCCCCCHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCH
EHVKYILKVSYNIGADTSLVLYGLTKGARSMIESLNVEKKILDKKYGISPSEYHFIDGSG
HHEEEEEEEEECCCCCCEEEEEECCHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEECCC
GGETKATNTAIIKWLTIMSKRPVFNIFYNSLPILAVDGSLGSINNFQSDPSLSGATGRVH
CCCCCCCHHHHHHHHHHHCCCCEEEEHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCEEE
AKTGTYVLENESGMLVKGRSLAGYIISKKNHRLAFALVVNNIPIQSLEDLFAVAQDQGII
ECCCCEEEECCCCCEEECCCEEEEEEECCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCE
ASILWRDY
EEHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: Preferential cleavage is (Ac)2-L-Lys-D-Ala D-Ala and Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine [C]

General reaction: Hydrolase; Acting on peptide bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA