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The map label for this gene is bioB [H]

Identifier: 54294487

GI number: 54294487

Start: 1733038

End: 1733985

Strand: Reverse

Name: bioB [H]

Synonym: lpl1556

Alternate gene names: 54294487

Gene position: 1733985-1733038 (Counterclockwise)

Preceding gene: 54294489

Following gene: 54294486

Centisome position: 51.83

GC content: 38.71

Gene sequence:

>948_bases
GTGTCTGAAGAAAAAAAACAGTGGTCAATTTCTGATATTGAAGCAATTTATCAACAACCATTTAATGATTTACTTTATCA
AGCACATGCCATTCATAGGACATATCACGATCCTAATTCTTTACAATTTGCAACCTTGTTAAGCATTAAAACGGGCGCAT
GCCCGGAAGATTGTGGCTATTGCTCTCAAAGTGGTCACTATAAAACGCATGTAGAAAAAGAAAAATTGATGTCAGTTGAA
GAGGTATTGCAATGTGCCAAAGAAGCAAAGGAGGGTGGGGCCAAACGTTTTTGTATGGGAGCTGCATGGCGTTGTCCACC
TGATAAAGCAATTCCTCAGCTAAAAGAAATGATAGAAGGAGTAAAGTCTTTAGGGCTTGAAACCTGTATGACTTTAGGCA
TGCTTACCAAAGAACAGGCCTCTCATTTAAAGGAGGCTGGATTGGATTATTATAACCATAATATTGATACCTCACCATCC
TATTACGATAAGGTGGTAACCACTCGAAAATTTAGTGATCGATTAGATACTTTAAATAACGTCCGTTCAGCGGGAATTAA
TGTATGTTGTGGTGGTATTTTAGGATTGGGAGAAACCAGAGAAGATCGAATCGAATTTTTATTAACATTAGCTAATATGG
AAACTCCTCCGGAAAGTGTGCCAATTAATCGTTTAATTCCAGTGGAAGGAACACCTTTAGCCCAAGCTGAGAGAGTAGAA
GGGATTGAATTGGTAAGAACCATTGCTACAGCAAGAATTTTAATGCCTAAAAGTGCTATTAGATTGACAGCCGGCCGAAC
CGAAATGAGCGACGAGTTACAAGCACTTTGCTATTTTGCCGGAGCCAATTCTGTGTTTATTGGCGATAAATTATTGACAG
AAGATAATCCCCAGCGCTTCAAAGACAAAACTCTTTTTAACAAACTGGGTTTGACTGAGATGGTATAA

Upstream 100 bases:

>100_bases
TTGCAATCAGTTGATCTGTGTTTACCATTTTAAAAATTTTGGTTGACAAGTATAGGCGCGACTTTATCATGTTCACTATT
CTGAATTAAAGGGGGTAGCC

Downstream 100 bases:

>100_bases
ATGACTCTTAATATAAAAATTAATGAGTACACAGCACAGCTGCAAGAGCAAGGATTATTGCGTACAAGACAAATTTTAGA
AACGGATAAGATAGATTATA

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MSEEKKQWSISDIEAIYQQPFNDLLYQAHAIHRTYHDPNSLQFATLLSIKTGACPEDCGYCSQSGHYKTHVEKEKLMSVE
EVLQCAKEAKEGGAKRFCMGAAWRCPPDKAIPQLKEMIEGVKSLGLETCMTLGMLTKEQASHLKEAGLDYYNHNIDTSPS
YYDKVVTTRKFSDRLDTLNNVRSAGINVCCGGILGLGETREDRIEFLLTLANMETPPESVPINRLIPVEGTPLAQAERVE
GIELVRTIATARILMPKSAIRLTAGRTEMSDELQALCYFAGANSVFIGDKLLTEDNPQRFKDKTLFNKLGLTEMV

Sequences:

>Translated_315_residues
MSEEKKQWSISDIEAIYQQPFNDLLYQAHAIHRTYHDPNSLQFATLLSIKTGACPEDCGYCSQSGHYKTHVEKEKLMSVE
EVLQCAKEAKEGGAKRFCMGAAWRCPPDKAIPQLKEMIEGVKSLGLETCMTLGMLTKEQASHLKEAGLDYYNHNIDTSPS
YYDKVVTTRKFSDRLDTLNNVRSAGINVCCGGILGLGETREDRIEFLLTLANMETPPESVPINRLIPVEGTPLAQAERVE
GIELVRTIATARILMPKSAIRLTAGRTEMSDELQALCYFAGANSVFIGDKLLTEDNPQRFKDKTLFNKLGLTEMV
>Mature_314_residues
SEEKKQWSISDIEAIYQQPFNDLLYQAHAIHRTYHDPNSLQFATLLSIKTGACPEDCGYCSQSGHYKTHVEKEKLMSVEE
VLQCAKEAKEGGAKRFCMGAAWRCPPDKAIPQLKEMIEGVKSLGLETCMTLGMLTKEQASHLKEAGLDYYNHNIDTSPSY
YDKVVTTRKFSDRLDTLNNVRSAGINVCCGGILGLGETREDRIEFLLTLANMETPPESVPINRLIPVEGTPLAQAERVEG
IELVRTIATARILMPKSAIRLTAGRTEMSDELQALCYFAGANSVFIGDKLLTEDNPQRFKDKTLFNKLGLTEMV

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=307, Percent_Identity=56.3517915309446, Blast_Score=389, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6321725, Length=322, Percent_Identity=47.2049689440994, Blast_Score=320, Evalue=2e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 35148; Mature: 35016

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEEKKQWSISDIEAIYQQPFNDLLYQAHAIHRTYHDPNSLQFATLLSIKTGACPEDCGY
CCCCHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHHEEECCCCCCHHHCC
CSQSGHYKTHVEKEKLMSVEEVLQCAKEAKEGGAKRFCMGAAWRCPPDKAIPQLKEMIEG
CCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEHHCCCCCCCCHHHHHHHHHHHHH
VKSLGLETCMTLGMLTKEQASHLKEAGLDYYNHNIDTSPSYYDKVVTTRKFSDRLDTLNN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VRSAGINVCCGGILGLGETREDRIEFLLTLANMETPPESVPINRLIPVEGTPLAQAERVE
HHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCHHHHHHH
GIELVRTIATARILMPKSAIRLTAGRTEMSDELQALCYFAGANSVFIGDKLLTEDNPQRF
HHHHHHHHHHHHHHCCHHHHEEECCCCHHHHHHHHHHHHCCCCEEEECCCHHCCCCHHHH
KDKTLFNKLGLTEMV
HHHHHHHHCCHHHCC
>Mature Secondary Structure 
SEEKKQWSISDIEAIYQQPFNDLLYQAHAIHRTYHDPNSLQFATLLSIKTGACPEDCGY
CCCHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHHEEECCCCCCHHHCC
CSQSGHYKTHVEKEKLMSVEEVLQCAKEAKEGGAKRFCMGAAWRCPPDKAIPQLKEMIEG
CCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEHHCCCCCCCCHHHHHHHHHHHHH
VKSLGLETCMTLGMLTKEQASHLKEAGLDYYNHNIDTSPSYYDKVVTTRKFSDRLDTLNN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VRSAGINVCCGGILGLGETREDRIEFLLTLANMETPPESVPINRLIPVEGTPLAQAERVE
HHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCHHHHHHH
GIELVRTIATARILMPKSAIRLTAGRTEMSDELQALCYFAGANSVFIGDKLLTEDNPQRF
HHHHHHHHHHHHHHCCHHHHEEECCCCHHHHHHHHHHHHCCCCEEEECCCHHCCCCHHHH
KDKTLFNKLGLTEMV
HHHHHHHHCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA