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The map label for this gene is pilB [H]

Identifier: 54294435

GI number: 54294435

Start: 1673697

End: 1675421

Strand: Reverse

Name: pilB [H]

Synonym: lpl1504

Alternate gene names: 54294435

Gene position: 1675421-1673697 (Counterclockwise)

Preceding gene: 54294439

Following gene: 54294434

Centisome position: 50.08

GC content: 39.42

Gene sequence:

>1725_bases
ATGGCTCTAGCTACAGAAGAATATAGATTACAGGGAATCGGCCAGCTTCTCGTGCTGGAAAAGATGCTAGATAAAGCAAA
AGCAATTGAACTACACAAGCTGGCAGCAGCAGAAAAGATGTCTTTGCTGCAATACATTGTAAAAAATAAAATATTATCTG
CTGAACAAATTGCACTGACAGCTGCTCAAAATTTTGGCGTACCCATGTTGGATATTAACTGCATTGATGTAGGCACCATT
CCTGCCAATCTGGTTAATGAGAAATTAATTAAACGTCATGCCATGGTTCCTCTTTTTAGTCGCGGTACCAATTTATATCT
CGCAACAGATGATCCTAGTAAACAAGCTTCATTAAAGGAAATTCAATTTCACACCGGATTAAATACTCATGCGATAGTTG
TAGAAACAGATAAACTTAGTGCCCTGATTGATAACCTGTTAACAGCAAAGGAAAGTCAGGGCTTATCAGAGTATGTTGAA
GACTCTGGAGATCTGGAAGGTTTAGAAATAAGTGCTGATGATGAAGATCAAGATAGTGATACTGCAACATCAGTAACTGA
TGATGCGCCTATCGTAAAATTCGTCAATAAAATTTTATTGGATGCGATAAGGCAAGGAGCTTCTGATATACACTTTGAGC
CTTATGAACGAGAATACCGAATCAGATACAGACAAGATGGTATTTTACATGAAGTCGCTACCCCTCCCGCAAGCTTGTCA
TCTCGTATTACAGCACGTATCAAGGTAATGTCTAATTTGGATATATCAGAACGGCGTATCCCCCAGGATGGTGGCTTCAA
AATGAAAATTTCCAAGTCACGAGCAATTGATTTCAGAGTGAGTACATGTCCAACTTCGGCTGGGGAAAAAGTGGTTATGC
GGGTTCTTGATTCTGGTGCTGCTAAATTAGGTATTGAAGCATTGGGATTTAATCCTGTTCAGAGAACAAATTTCCTGAAA
GCAATCCAGCGCCCACAAGGGATGATTTTAGTCACTGGCCCTACTGGTAGTGGTAAAACAGTGACTTTATATACGGCTTT
AAATATATTAAATACAATTGAGGTTAATATTTCAACAGCTGAAGATCCAGTTGAAATCAAAGTTCCTGGTATCAATCAGG
TTAATATTAATCCTAAAGCTGGCCTAACCTTTTCCGGTGCATTACGTTCGTTTCTAAGGCAAGACCCTGATATTATAATG
GTTGGTGAGATACGAGACCTTGAAACAGCAGAGATTGCTGTAAAAGCTGCTCAGACAGGGCATTTGGTTCTTTCTACTCT
GCACACAAACAGTGCCGCTGAAACTTTAAATCGTTTAGTAAACATGGGAATACCAACTTTTAACATTGCGAGTTCGGTTA
CCTTAATTATTGCGCAAAGGCTGGCAAGAAAATTATGCAATCAATGTAAGGCAGTGCGAGATGACTTTACCAATCAAGGT
TTGATTGAACTAGGTTTCAAGGAATCTGATCTGGTTAATCTTAAATTATACAAAGCAGTTGGTTGCGAGCAATGTACAAG
TGGTTATCGCGGACGAGTCGGGTTGTTTGAGGTTTTACCGATGACGAAAGAGCTTGGCCAATTGATTATGTCAGGTGGTA
ATTCGCTGGATATATTAAAGTTAGCTCAATCAGAAGGTATGCTGACTATTTTTCAATCGGGTATAGAAAAAGTTAAGGAA
GGAATCACGACCATAGAGGAGGTCAATCGGGTAACCGTTGATTAA

Upstream 100 bases:

>100_bases
TTGGGGGCAGGTCCAAAACATCGGGTTTTGTTCAAAAAAACATGCAATTATAAAAAAATTTAACTATATTTAAAACTAAG
ATACACTTTGAATAAATGAA

Downstream 100 bases:

>100_bases
TTTGGATGGATAAAAATAGCCCACCCCTATTAACCTTTCATTATCAAGGCATTAATAAAGCCGGTCAAAAAATGGAGGGT
GATATTCAAGCCAGAAGCTT

Product: pilus assembly protein PilB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 574; Mature: 573

Protein sequence:

>574_residues
MALATEEYRLQGIGQLLVLEKMLDKAKAIELHKLAAAEKMSLLQYIVKNKILSAEQIALTAAQNFGVPMLDINCIDVGTI
PANLVNEKLIKRHAMVPLFSRGTNLYLATDDPSKQASLKEIQFHTGLNTHAIVVETDKLSALIDNLLTAKESQGLSEYVE
DSGDLEGLEISADDEDQDSDTATSVTDDAPIVKFVNKILLDAIRQGASDIHFEPYEREYRIRYRQDGILHEVATPPASLS
SRITARIKVMSNLDISERRIPQDGGFKMKISKSRAIDFRVSTCPTSAGEKVVMRVLDSGAAKLGIEALGFNPVQRTNFLK
AIQRPQGMILVTGPTGSGKTVTLYTALNILNTIEVNISTAEDPVEIKVPGINQVNINPKAGLTFSGALRSFLRQDPDIIM
VGEIRDLETAEIAVKAAQTGHLVLSTLHTNSAAETLNRLVNMGIPTFNIASSVTLIIAQRLARKLCNQCKAVRDDFTNQG
LIELGFKESDLVNLKLYKAVGCEQCTSGYRGRVGLFEVLPMTKELGQLIMSGGNSLDILKLAQSEGMLTIFQSGIEKVKE
GITTIEEVNRVTVD

Sequences:

>Translated_574_residues
MALATEEYRLQGIGQLLVLEKMLDKAKAIELHKLAAAEKMSLLQYIVKNKILSAEQIALTAAQNFGVPMLDINCIDVGTI
PANLVNEKLIKRHAMVPLFSRGTNLYLATDDPSKQASLKEIQFHTGLNTHAIVVETDKLSALIDNLLTAKESQGLSEYVE
DSGDLEGLEISADDEDQDSDTATSVTDDAPIVKFVNKILLDAIRQGASDIHFEPYEREYRIRYRQDGILHEVATPPASLS
SRITARIKVMSNLDISERRIPQDGGFKMKISKSRAIDFRVSTCPTSAGEKVVMRVLDSGAAKLGIEALGFNPVQRTNFLK
AIQRPQGMILVTGPTGSGKTVTLYTALNILNTIEVNISTAEDPVEIKVPGINQVNINPKAGLTFSGALRSFLRQDPDIIM
VGEIRDLETAEIAVKAAQTGHLVLSTLHTNSAAETLNRLVNMGIPTFNIASSVTLIIAQRLARKLCNQCKAVRDDFTNQG
LIELGFKESDLVNLKLYKAVGCEQCTSGYRGRVGLFEVLPMTKELGQLIMSGGNSLDILKLAQSEGMLTIFQSGIEKVKE
GITTIEEVNRVTVD
>Mature_573_residues
ALATEEYRLQGIGQLLVLEKMLDKAKAIELHKLAAAEKMSLLQYIVKNKILSAEQIALTAAQNFGVPMLDINCIDVGTIP
ANLVNEKLIKRHAMVPLFSRGTNLYLATDDPSKQASLKEIQFHTGLNTHAIVVETDKLSALIDNLLTAKESQGLSEYVED
SGDLEGLEISADDEDQDSDTATSVTDDAPIVKFVNKILLDAIRQGASDIHFEPYEREYRIRYRQDGILHEVATPPASLSS
RITARIKVMSNLDISERRIPQDGGFKMKISKSRAIDFRVSTCPTSAGEKVVMRVLDSGAAKLGIEALGFNPVQRTNFLKA
IQRPQGMILVTGPTGSGKTVTLYTALNILNTIEVNISTAEDPVEIKVPGINQVNINPKAGLTFSGALRSFLRQDPDIIMV
GEIRDLETAEIAVKAAQTGHLVLSTLHTNSAAETLNRLVNMGIPTFNIASSVTLIIAQRLARKLCNQCKAVRDDFTNQGL
IELGFKESDLVNLKLYKAVGCEQCTSGYRGRVGLFEVLPMTKELGQLIMSGGNSLDILKLAQSEGMLTIFQSGIEKVKEG
ITTIEEVNRVTVD

Specific function: Involved in the translocation of the type IV pilin [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1786296, Length=382, Percent_Identity=49.738219895288, Blast_Score=382, Evalue=1e-107,
Organism=Escherichia coli, GI1789723, Length=481, Percent_Identity=41.5800415800416, Blast_Score=343, Evalue=2e-95,
Organism=Escherichia coli, GI87082188, Length=382, Percent_Identity=30.8900523560209, Blast_Score=120, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 62677; Mature: 62546

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALATEEYRLQGIGQLLVLEKMLDKAKAIELHKLAAAEKMSLLQYIVKNKILSAEQIALT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEE
AAQNFGVPMLDINCIDVGTIPANLVNEKLIKRHAMVPLFSRGTNLYLATDDPSKQASLKE
HHHCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHCCEEECCCCEEEEEECCCCCCCHHHH
IQFHTGLNTHAIVVETDKLSALIDNLLTAKESQGLSEYVEDSGDLEGLEISADDEDQDSD
EEEECCCCCEEEEEECHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCEEECCCCCCCCCC
TATSVTDDAPIVKFVNKILLDAIRQGASDIHFEPYEREYRIRYRQDGILHEVATPPASLS
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHCCCCHHHH
SRITARIKVMSNLDISERRIPQDGGFKMKISKSRAIDFRVSTCPTSAGEKVVMRVLDSGA
HHHEEEEEEEECCCHHHHCCCCCCCEEEEEECCCEEEEEEECCCCCCCHHHHHHHHHCCC
AKLGIEALGFNPVQRTNFLKAIQRPQGMILVTGPTGSGKTVTLYTALNILNTIEVNISTA
HHCCCCEECCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEEEHHHHEEEEEEEECC
EDPVEIKVPGINQVNINPKAGLTFSGALRSFLRQDPDIIMVGEIRDLETAEIAVKAAQTG
CCCEEEEECCCCEEECCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHEEEECCCC
HLVLSTLHTNSAAETLNRLVNMGIPTFNIASSVTLIIAQRLARKLCNQCKAVRDDFTNQG
CEEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LIELGFKESDLVNLKLYKAVGCEQCTSGYRGRVGLFEVLPMTKELGQLIMSGGNSLDILK
EEEECCCCCCEEEEEEEHHCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEE
LAQSEGMLTIFQSGIEKVKEGITTIEEVNRVTVD
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ALATEEYRLQGIGQLLVLEKMLDKAKAIELHKLAAAEKMSLLQYIVKNKILSAEQIALT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEE
AAQNFGVPMLDINCIDVGTIPANLVNEKLIKRHAMVPLFSRGTNLYLATDDPSKQASLKE
HHHCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHCCEEECCCCEEEEEECCCCCCCHHHH
IQFHTGLNTHAIVVETDKLSALIDNLLTAKESQGLSEYVEDSGDLEGLEISADDEDQDSD
EEEECCCCCEEEEEECHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCEEECCCCCCCCCC
TATSVTDDAPIVKFVNKILLDAIRQGASDIHFEPYEREYRIRYRQDGILHEVATPPASLS
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHCCCCHHHH
SRITARIKVMSNLDISERRIPQDGGFKMKISKSRAIDFRVSTCPTSAGEKVVMRVLDSGA
HHHEEEEEEEECCCHHHHCCCCCCCEEEEEECCCEEEEEEECCCCCCCHHHHHHHHHCCC
AKLGIEALGFNPVQRTNFLKAIQRPQGMILVTGPTGSGKTVTLYTALNILNTIEVNISTA
HHCCCCEECCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEEEHHHHEEEEEEEECC
EDPVEIKVPGINQVNINPKAGLTFSGALRSFLRQDPDIIMVGEIRDLETAEIAVKAAQTG
CCCEEEEECCCCEEECCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHEEEECCCC
HLVLSTLHTNSAAETLNRLVNMGIPTFNIASSVTLIIAQRLARKLCNQCKAVRDDFTNQG
CEEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LIELGFKESDLVNLKLYKAVGCEQCTSGYRGRVGLFEVLPMTKELGQLIMSGGNSLDILK
EEEECCCCCCEEEEEEEHHCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEE
LAQSEGMLTIFQSGIEKVKEGITTIEEVNRVTVD
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8820654 [H]