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The map label for this gene is rpoE [H]

Identifier: 54294379

GI number: 54294379

Start: 1616724

End: 1617287

Strand: Direct

Name: rpoE [H]

Synonym: lpl1448

Alternate gene names: 54294379

Gene position: 1616724-1617287 (Clockwise)

Preceding gene: 54294378

Following gene: 54294380

Centisome position: 48.32

GC content: 32.98

Gene sequence:

>564_bases
ATGCATATTGAGACCTCTTTAAGTGATGAATCTCTAATTGAATTAGCTCAAAAAGGCAACGGTCTTGCTTTTGATAAACT
GTTTGAGCGTTATCAGGGCAAAATTAGACAAATGATTTATTCTTACACGTATGACTGGACAAATGTCAATGATTTAACTC
AGGAAGTTCTTATCAAAGTATTTAGGCATTTACCTTATTTTAATGAACGAAGTCAGTTTTCAACCTGGCTATATAAAATT
ACTCAAAATACTATTAAAAATTATTTTCGTACCAATAATTTATATGCTGATTCAGAAATGCAGTATATGGATGAACAATT
TTCTACACCACATAATTCTCCAGAGTCAGACATAATGGGATTAGAGTTTGAGAAACAAATAGAAGCAGCAATGTCATGTT
TATCTGAAGAGTTACGATTATGTTATGGGCTGCATATGTTTGATGGACAAACTTATCAGGATATTGCAAAAGAAATGCAT
TGCCCCATCGGAACTGTACGCTCACGTATATTTAGAGCTCGAAAACAGCTGATGGATATTGTAAGTGAGAATCACACTTT
ATAA

Upstream 100 bases:

>100_bases
TATATAATAATACATAATGATTAAAATAAATCCAAGTTAACAATGATTATACTTTATCTTATCCTAACGTAATAACAAGC
AAAGGAATAATGAGGAAGCT

Downstream 100 bases:

>100_bases
TATCATTTTGATATCATATAAAATCATCAACCTTAATCGTGAAATCAATTGCATTTAATTATTAAATAAATAAGCTTACA
AAGATCTGCTATTAGATAAA

Product: sigma factor RpoE (sigma 24)

Products: NA

Alternate protein names: Sigma-30 [H]

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MHIETSLSDESLIELAQKGNGLAFDKLFERYQGKIRQMIYSYTYDWTNVNDLTQEVLIKVFRHLPYFNERSQFSTWLYKI
TQNTIKNYFRTNNLYADSEMQYMDEQFSTPHNSPESDIMGLEFEKQIEAAMSCLSEELRLCYGLHMFDGQTYQDIAKEMH
CPIGTVRSRIFRARKQLMDIVSENHTL

Sequences:

>Translated_187_residues
MHIETSLSDESLIELAQKGNGLAFDKLFERYQGKIRQMIYSYTYDWTNVNDLTQEVLIKVFRHLPYFNERSQFSTWLYKI
TQNTIKNYFRTNNLYADSEMQYMDEQFSTPHNSPESDIMGLEFEKQIEAAMSCLSEELRLCYGLHMFDGQTYQDIAKEMH
CPIGTVRSRIFRARKQLMDIVSENHTL
>Mature_187_residues
MHIETSLSDESLIELAQKGNGLAFDKLFERYQGKIRQMIYSYTYDWTNVNDLTQEVLIKVFRHLPYFNERSQFSTWLYKI
TQNTIKNYFRTNNLYADSEMQYMDEQFSTPHNSPESDIMGLEFEKQIEAAMSCLSEELRLCYGLHMFDGQTYQDIAKEMH
CPIGTVRSRIFRARKQLMDIVSENHTL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor regulates genes such as algD, involved in alginate biosynthesis [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=178, Percent_Identity=37.0786516853933, Blast_Score=114, Evalue=3e-27,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR014286
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 22189; Mature: 22189

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIETSLSDESLIELAQKGNGLAFDKLFERYQGKIRQMIYSYTYDWTNVNDLTQEVLIKV
CCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
FRHLPYFNERSQFSTWLYKITQNTIKNYFRTNNLYADSEMQYMDEQFSTPHNSPESDIMG
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCC
LEFEKQIEAAMSCLSEELRLCYGLHMFDGQTYQDIAKEMHCPIGTVRSRIFRARKQLMDI
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
VSENHTL
HHCCCCC
>Mature Secondary Structure
MHIETSLSDESLIELAQKGNGLAFDKLFERYQGKIRQMIYSYTYDWTNVNDLTQEVLIKV
CCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
FRHLPYFNERSQFSTWLYKITQNTIKNYFRTNNLYADSEMQYMDEQFSTPHNSPESDIMG
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCC
LEFEKQIEAAMSCLSEELRLCYGLHMFDGQTYQDIAKEMHCPIGTVRSRIFRARKQLMDI
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
VSENHTL
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432708; 8378309; 7961421; 10984043; 7737518 [H]