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The map label for this gene is proB [H]

Identifier: 54294346

GI number: 54294346

Start: 1578481

End: 1579551

Strand: Direct

Name: proB [H]

Synonym: lpl1414

Alternate gene names: 54294346

Gene position: 1578481-1579551 (Clockwise)

Preceding gene: 54294345

Following gene: 54294347

Centisome position: 47.18

GC content: 33.99

Gene sequence:

>1071_bases
ATGAAAATTATTATAAAAGTCGGAACACAAAGTATTTTATCGGTCGACGGAAGTCCAATCTATTCCGTACTAAATAATCT
TGTAAATCAAATTATTTATTTAAAGAACCTGGGGCATCAGGTCATTTTAGTAAGCTCTGGGGCTGTGGGCTCTGGTCGAA
AAATAGCTAAGAACTCTCTGGGGAAAACATATGGCGATACAGTGGGAGAGAAGCAACTCTTGGCTTCAATAGGCCAACCC
GAACTGATGAATCTCTATTCTAGCTTGTTTAAAAATGAAAACATTTTAGTATCTCAGTTATTACTTACCAAACAAGACTT
CCATACTAGACAGCATTATTTAAATATTGCCCGTTTATTAACGGAATTACTAAAGCATCAAAATATTATCCCTATTGTTA
ATGAAAATGATTCGGTAGCCATTGAAGAATTAATGTTTACTGATAATGATGAATTAGCAGGACTTATTGCAGCTCAAATT
AATGCTGATTATCTAATACTCTTAACCAATGTCGATGGTGTGTTTACATCCCATCCCAATGATCCTGAATCAAAACTCAT
TAATACAATTGACCCCAATTCAGGTTGGCCCAATGTCTCTGTTGTAAAATCAAAATTAGGCCGAGGTGGTATGGTATCCA
AACTGGATACTGCCAGAAAAATGGCTGGTTTAGGTATCAATACAGTGATCGCAAATGTAAATGTAGAATCCATTGTTCCA
AAAATTCTAAATAGGGAAGCAGTTGGAACTATAATTCTTGCTAGTAAAAGAAAATCCAGTATAAAAAGATGGATTGCTTT
TAGCCATAGAACTTCCTTACCATCTATAGTAATTAATGATAATTTAATTACCTTATTACGAAGTGGTAATCGTGTAATGA
GCATTTTACCAATAGGGATAGAAGATTTTCGTGGAGAATGCAAAAAAGGGGATTTAATTGATATCCTTAATTCCAATAAT
GAAAAGATAGGAGTTGGAATCGCCAGGTATGATTTCTTAAGATTAAAGGACTATCTTAGACTTCAAAATAAGCCTGCATT
TATACATTACGATCAATTGCATATTCTATAG

Upstream 100 bases:

>100_bases
CCCAGAATAAGTATTATGGGATCTTTATGATTATTGGAAAGACAAATTTCAAGCATACTGATATTCTTGATATTAAGAAA
AGCTTGTTTAGAAATATGAA

Downstream 100 bases:

>100_bases
GTATCAAAATGAATACTGATATTGCTAACCAATTAAGAGCCGCTAAAAAAGCAACTACTGATCTTAACTTAATACAAAGT
GATACCCGAACAATAATTTT

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MKIIIKVGTQSILSVDGSPIYSVLNNLVNQIIYLKNLGHQVILVSSGAVGSGRKIAKNSLGKTYGDTVGEKQLLASIGQP
ELMNLYSSLFKNENILVSQLLLTKQDFHTRQHYLNIARLLTELLKHQNIIPIVNENDSVAIEELMFTDNDELAGLIAAQI
NADYLILLTNVDGVFTSHPNDPESKLINTIDPNSGWPNVSVVKSKLGRGGMVSKLDTARKMAGLGINTVIANVNVESIVP
KILNREAVGTIILASKRKSSIKRWIAFSHRTSLPSIVINDNLITLLRSGNRVMSILPIGIEDFRGECKKGDLIDILNSNN
EKIGVGIARYDFLRLKDYLRLQNKPAFIHYDQLHIL

Sequences:

>Translated_356_residues
MKIIIKVGTQSILSVDGSPIYSVLNNLVNQIIYLKNLGHQVILVSSGAVGSGRKIAKNSLGKTYGDTVGEKQLLASIGQP
ELMNLYSSLFKNENILVSQLLLTKQDFHTRQHYLNIARLLTELLKHQNIIPIVNENDSVAIEELMFTDNDELAGLIAAQI
NADYLILLTNVDGVFTSHPNDPESKLINTIDPNSGWPNVSVVKSKLGRGGMVSKLDTARKMAGLGINTVIANVNVESIVP
KILNREAVGTIILASKRKSSIKRWIAFSHRTSLPSIVINDNLITLLRSGNRVMSILPIGIEDFRGECKKGDLIDILNSNN
EKIGVGIARYDFLRLKDYLRLQNKPAFIHYDQLHIL
>Mature_356_residues
MKIIIKVGTQSILSVDGSPIYSVLNNLVNQIIYLKNLGHQVILVSSGAVGSGRKIAKNSLGKTYGDTVGEKQLLASIGQP
ELMNLYSSLFKNENILVSQLLLTKQDFHTRQHYLNIARLLTELLKHQNIIPIVNENDSVAIEELMFTDNDELAGLIAAQI
NADYLILLTNVDGVFTSHPNDPESKLINTIDPNSGWPNVSVVKSKLGRGGMVSKLDTARKMAGLGINTVIANVNVESIVP
KILNREAVGTIILASKRKSSIKRWIAFSHRTSLPSIVINDNLITLLRSGNRVMSILPIGIEDFRGECKKGDLIDILNSNN
EKIGVGIARYDFLRLKDYLRLQNKPAFIHYDQLHIL

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=260, Percent_Identity=32.3076923076923, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI62912457, Length=258, Percent_Identity=32.5581395348837, Blast_Score=118, Evalue=8e-27,
Organism=Escherichia coli, GI1786437, Length=369, Percent_Identity=32.520325203252, Blast_Score=159, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17569969, Length=268, Percent_Identity=33.955223880597, Blast_Score=130, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI17569967, Length=268, Percent_Identity=33.955223880597, Blast_Score=130, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6321822, Length=250, Percent_Identity=36, Blast_Score=128, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6320506, Length=248, Percent_Identity=33.8709677419355, Blast_Score=128, Evalue=2e-30,
Organism=Drosophila melanogaster, GI21357643, Length=324, Percent_Identity=28.3950617283951, Blast_Score=120, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 39351; Mature: 39351

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIIIKVGTQSILSVDGSPIYSVLNNLVNQIIYLKNLGHQVILVSSGAVGSGRKIAKNSL
CEEEEEECCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHC
GKTYGDTVGEKQLLASIGQPELMNLYSSLFKNENILVSQLLLTKQDFHTRQHYLNIARLL
CCHHCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TELLKHQNIIPIVNENDSVAIEELMFTDNDELAGLIAAQINADYLILLTNVDGVFTSHPN
HHHHHCCCEEEEECCCCCEEEEHEEECCCCHHHEEEEEECCCCEEEEEECCCCEECCCCC
DPESKLINTIDPNSGWPNVSVVKSKLGRGGMVSKLDTARKMAGLGINTVIANVNVESIVP
CHHHHHEECCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHEEECCCHHHHHH
KILNREAVGTIILASKRKSSIKRWIAFSHRTSLPSIVINDNLITLLRSGNRVMSILPIGI
HHHCCHHHEEEEEECCHHHHHHHHHHHHCCCCCCEEEECCCEEEEECCCCEEEEEEECCH
EDFRGECKKGDLIDILNSNNEKIGVGIARYDFLRLKDYLRLQNKPAFIHYDQLHIL
HHHCCCCCCCCEEEEECCCCCEEEEEHHHHHHHHHHHHHHHCCCCCEEEECCEEEC
>Mature Secondary Structure
MKIIIKVGTQSILSVDGSPIYSVLNNLVNQIIYLKNLGHQVILVSSGAVGSGRKIAKNSL
CEEEEEECCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHC
GKTYGDTVGEKQLLASIGQPELMNLYSSLFKNENILVSQLLLTKQDFHTRQHYLNIARLL
CCHHCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TELLKHQNIIPIVNENDSVAIEELMFTDNDELAGLIAAQINADYLILLTNVDGVFTSHPN
HHHHHCCCEEEEECCCCCEEEEHEEECCCCHHHEEEEEECCCCEEEEEECCCCEECCCCC
DPESKLINTIDPNSGWPNVSVVKSKLGRGGMVSKLDTARKMAGLGINTVIANVNVESIVP
CHHHHHEECCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHEEECCCHHHHHH
KILNREAVGTIILASKRKSSIKRWIAFSHRTSLPSIVINDNLITLLRSGNRVMSILPIGI
HHHCCHHHEEEEEECCHHHHHHHHHHHHCCCCCCEEEECCCEEEEECCCCEEEEEEECCH
EDFRGECKKGDLIDILNSNNEKIGVGIARYDFLRLKDYLRLQNKPAFIHYDQLHIL
HHHCCCCCCCCEEEEECCCCCEEEEEHHHHHHHHHHHHHHHCCCCCEEEECCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901 [H]