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The map label for this gene is truA [H]

Identifier: 54294200

GI number: 54294200

Start: 1407787

End: 1408575

Strand: Direct

Name: truA [H]

Synonym: lpl1265

Alternate gene names: 54294200

Gene position: 1407787-1408575 (Clockwise)

Preceding gene: 54294195

Following gene: 54294201

Centisome position: 42.08

GC content: 41.44

Gene sequence:

>789_bases
ATGCGTATTGCCTTAGTGGTTGAATACGATGGCAGCCAATATCACGGCTGGCAGGCTCAAACGGGTTTGCATACTATTCA
ACAAGCTGTTGAATTTGCATTATCAAAAGTTGCAGACAGTTCTATTTCTGTTGTCTGCGCAGGTAGAACAGATACTGGGG
TCCATGCGACCAATCAGGTGATTCATTTTGATTGTGAGAAAGATCGCAGCATACGAGCCTGGATACATGGTGCTAATAGC
TTTTTACCTAAAGACATCTGCGTCAAATGGGGCAAGGAAATGCCAGAAAATTTTCATGCTCGTTATTCTGCTGTGAGTCG
ACGGTATCGCTATGTCATTTATAATGGAGCTATACGACCTGGGTTATTACGCGGTAATGTGACATGGCAATATCGGCAGT
TGGATCATCGTTTGATGCAACAGGGTGGACAATGTCTTCTGGGGGAAAATGATTTTACTTCATTCCGCTCCGTCGAATGT
CAATCTAATACACCAATGCGTAATATTCATCAATTGCAAGTTACAAGACATGGTGATTTGGTTGTTCTTGATATCACAGC
GAATGCCTTTTTACATCATATGGTAAGAAATATAGCTGGTGTATTGATTGCTGTTGGCTCGGGAAAACATCCTGTTGGTT
GGGTTAAAGATGTTTTAAATGCAAAGGATAGAAAATTAGGCGCTGAAACTGCACCGTCATATGGCCTATATTTGGTCCAG
GTGACTTATCCCAAAGAATTTGGATTGTTGCAAAATAATCCTGGCCCTTTGTTTCTTTGGGAGAAATAA

Upstream 100 bases:

>100_bases
GCAAAATGATAAAACCCATACAGGCAAAGAGTTAATACTCTAAAATTATAGACAAAACATAGAGGGTGTTATAAATTGAC
CCTTTTATCAGGAAAATTGA

Downstream 100 bases:

>100_bases
TTGAATCCATCTCGTATAAGAGTCAAAATGTGTGGAATGACACGTTCAGAAGATATTCAATACGCCATTGATTTAGGGGT
GGACGCCATTGGCTTAATAT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MRIALVVEYDGSQYHGWQAQTGLHTIQQAVEFALSKVADSSISVVCAGRTDTGVHATNQVIHFDCEKDRSIRAWIHGANS
FLPKDICVKWGKEMPENFHARYSAVSRRYRYVIYNGAIRPGLLRGNVTWQYRQLDHRLMQQGGQCLLGENDFTSFRSVEC
QSNTPMRNIHQLQVTRHGDLVVLDITANAFLHHMVRNIAGVLIAVGSGKHPVGWVKDVLNAKDRKLGAETAPSYGLYLVQ
VTYPKEFGLLQNNPGPLFLWEK

Sequences:

>Translated_262_residues
MRIALVVEYDGSQYHGWQAQTGLHTIQQAVEFALSKVADSSISVVCAGRTDTGVHATNQVIHFDCEKDRSIRAWIHGANS
FLPKDICVKWGKEMPENFHARYSAVSRRYRYVIYNGAIRPGLLRGNVTWQYRQLDHRLMQQGGQCLLGENDFTSFRSVEC
QSNTPMRNIHQLQVTRHGDLVVLDITANAFLHHMVRNIAGVLIAVGSGKHPVGWVKDVLNAKDRKLGAETAPSYGLYLVQ
VTYPKEFGLLQNNPGPLFLWEK
>Mature_262_residues
MRIALVVEYDGSQYHGWQAQTGLHTIQQAVEFALSKVADSSISVVCAGRTDTGVHATNQVIHFDCEKDRSIRAWIHGANS
FLPKDICVKWGKEMPENFHARYSAVSRRYRYVIYNGAIRPGLLRGNVTWQYRQLDHRLMQQGGQCLLGENDFTSFRSVEC
QSNTPMRNIHQLQVTRHGDLVVLDITANAFLHHMVRNIAGVLIAVGSGKHPVGWVKDVLNAKDRKLGAETAPSYGLYLVQ
VTYPKEFGLLQNNPGPLFLWEK

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=274, Percent_Identity=29.5620437956204, Blast_Score=93, Evalue=2e-19,
Organism=Escherichia coli, GI1788657, Length=258, Percent_Identity=55.0387596899225, Blast_Score=301, Evalue=2e-83,
Organism=Drosophila melanogaster, GI19922728, Length=265, Percent_Identity=27.5471698113208, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24648455, Length=269, Percent_Identity=23.7918215613383, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI281362146, Length=269, Percent_Identity=23.7918215613383, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 29545; Mature: 29545

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIALVVEYDGSQYHGWQAQTGLHTIQQAVEFALSKVADSSISVVCAGRTDTGVHATNQV
CEEEEEEEECCCEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEE
IHFDCEKDRSIRAWIHGANSFLPKDICVKWGKEMPENFHARYSAVSRRYRYVIYNGAIRP
EEEECCCCCCEEEEEECCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHEEEEEEEECCCCC
GLLRGNVTWQYRQLDHRLMQQGGQCLLGENDFTSFRSVECQSNTPMRNIHQLQVTRHGDL
CEEECCCEEEHHHHHHHHHHCCCCEEECCCCCCCCEEEEECCCCCCCCEEEEEEEECCCE
VVLDITANAFLHHMVRNIAGVLIAVGSGKHPVGWVKDVLNAKDRKLGAETAPSYGLYLVQ
EEEEECHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHCCHHHCCCCCCCCCCCEEEEE
VTYPKEFGLLQNNPGPLFLWEK
EECCCCCCCEECCCCCEEEECC
>Mature Secondary Structure
MRIALVVEYDGSQYHGWQAQTGLHTIQQAVEFALSKVADSSISVVCAGRTDTGVHATNQV
CEEEEEEEECCCEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEE
IHFDCEKDRSIRAWIHGANSFLPKDICVKWGKEMPENFHARYSAVSRRYRYVIYNGAIRP
EEEECCCCCCEEEEEECCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHEEEEEEEECCCCC
GLLRGNVTWQYRQLDHRLMQQGGQCLLGENDFTSFRSVECQSNTPMRNIHQLQVTRHGDL
CEEECCCEEEHHHHHHHHHHCCCCEEECCCCCCCCEEEEECCCCCCCCEEEEEEEECCCE
VVLDITANAFLHHMVRNIAGVLIAVGSGKHPVGWVKDVLNAKDRKLGAETAPSYGLYLVQ
EEEEECHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHCCHHHCCCCCCCCCCCEEEEE
VTYPKEFGLLQNNPGPLFLWEK
EECCCCCCCEECCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA