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The map label for this gene is murE [H]

Identifier: 54293892

GI number: 54293892

Start: 1059810

End: 1061261

Strand: Direct

Name: murE [H]

Synonym: lpl0948

Alternate gene names: 54293892

Gene position: 1059810-1061261 (Clockwise)

Preceding gene: 54293891

Following gene: 54293895

Centisome position: 31.68

GC content: 41.12

Gene sequence:

>1452_bases
GTGAAACTAACCCAATTATTAGAACCGTGGATTAAAAGGGACATTATTGATGGCACTGTTAGTGACATAAAAAATGACAG
TCGCTTGGTGAAAGAGGGGGATTTATTTGCAGCCTATCCAGGGGCAGCTTCCGATGGTCGATTATATATAGAAAAGGCTG
TGGCATCAGGTGCTGCCGCTGTGGTTTATGAGCCTAATAATTTACCTCAGGGAATTACTTTGCCAACAGCGATTCCCTGC
ATAGCTATTCCCCAATTAGCTGAAAAACTAGGAGAAATAGCGAAACGGTTTTTTAATAACCCATCCCAATTTCTTGGTTT
AACTGGGGTAACTGGCACAAATGGCAAGACGACTATTGCTTATCAGTTGGCACAGGCACATGATTTACTCGGGCAGCACT
CGGCTTACATTGGGACCATAGGGCAAGGCAAGGTGGATGCCTTAAAACCTTTGGAAAATACTACGCCAGATGCTCTTTGT
TTGCAGAGGTTGATGCATCAGTATAAAGAAGAGGGCATTAAACAGGTTTGTATGGAGGTATCCTCTCATGCATTGAGCCA
GCATAGAGTCGACTCTCTCGAATTTAGTCAGGCTATCTTTACCAATTTAACGCTCGATCATTTAGATTATCACCAGACAA
TGCAAGCTTATGGGGCTGCAAAGTCGAGGTTGTTTGAACGTTCGGAGTTGCAATGGGCTATTATTAATCAGGATGACGAC
TGTCAAGATCTCATGAGCAATGTGCTAAAGACTCATGTAAAAAAAGTGACTTACGGTATGCATCAAAATTGCGATGTGAA
AGCGATCCAATGGAATATGGATATCACCGGAACAGAAATTGAAGTCAAATCTCCTTGGGGGCACCACCTTCTAAAGATTA
AAGCACTGGGTAAGTTTAATATTTATAATAGCCTGGCTGTTTATAGCAGTCTGCTTGCCAGCGGGTATAATCCAGAAAAA
GTAGTGCAAATTATGGCTGAATTAAAAGCGGCTCCTGGCCGAATGGAAATTGTCGCTCAATCCCCATATGTTCTTGTTGA
TTATGCTCATACTCCCGATGCATTGGAAAATGTTCTTATCACCTTAAAACAAATAAAAAAAGGGAGATTGTGGGTTGTAT
TTGGTTGTGGTGGGGACAGAGATAAAACCAAACGGCCCATTATGGGAAAGGCAGCCAGCATGTACGCTGATAATATTGTC
ATTACCAGCGATAATCCAAGAAGTGAAGATCCGGAAATTATTCTAAATGAAATTGCTTGTGGCATGCCTGCCTCTGCGCC
TGTTTCTAAACTGGTGAATAGAGAAGAAGCAATTGCTTATGCTTTAAGAAAGGCTGAACATAACGATATCATATTAATAG
CAGGAAAAGGACATGAATCGTATCAGCAGATTGGGAATACAAAACATGTTTTTTCTGATCAGGAAGTTGTAAGACGTTTG
ATGAATAATTAA

Upstream 100 bases:

>100_bases
TGGCAGGACCTTTATTTGCGAAAGTCATGTCAGGTGCTTTACGCTTATTCAATATTCCTACTGATGAGCCAGTTAGTTAA
AGAGAATAGGAGCCAATTTT

Downstream 100 bases:

>100_bases
AATTTCGGGGCAAGGATTAATTATTAGAAACAGACATCAACACCATTAACCTAATAATGGATACTTTTTTATTGAATCAG
AATCAAAATAAGTTGTGAAA

Product: hypothetical protein

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MKLTQLLEPWIKRDIIDGTVSDIKNDSRLVKEGDLFAAYPGAASDGRLYIEKAVASGAAAVVYEPNNLPQGITLPTAIPC
IAIPQLAEKLGEIAKRFFNNPSQFLGLTGVTGTNGKTTIAYQLAQAHDLLGQHSAYIGTIGQGKVDALKPLENTTPDALC
LQRLMHQYKEEGIKQVCMEVSSHALSQHRVDSLEFSQAIFTNLTLDHLDYHQTMQAYGAAKSRLFERSELQWAIINQDDD
CQDLMSNVLKTHVKKVTYGMHQNCDVKAIQWNMDITGTEIEVKSPWGHHLLKIKALGKFNIYNSLAVYSSLLASGYNPEK
VVQIMAELKAAPGRMEIVAQSPYVLVDYAHTPDALENVLITLKQIKKGRLWVVFGCGGDRDKTKRPIMGKAASMYADNIV
ITSDNPRSEDPEIILNEIACGMPASAPVSKLVNREEAIAYALRKAEHNDIILIAGKGHESYQQIGNTKHVFSDQEVVRRL
MNN

Sequences:

>Translated_483_residues
MKLTQLLEPWIKRDIIDGTVSDIKNDSRLVKEGDLFAAYPGAASDGRLYIEKAVASGAAAVVYEPNNLPQGITLPTAIPC
IAIPQLAEKLGEIAKRFFNNPSQFLGLTGVTGTNGKTTIAYQLAQAHDLLGQHSAYIGTIGQGKVDALKPLENTTPDALC
LQRLMHQYKEEGIKQVCMEVSSHALSQHRVDSLEFSQAIFTNLTLDHLDYHQTMQAYGAAKSRLFERSELQWAIINQDDD
CQDLMSNVLKTHVKKVTYGMHQNCDVKAIQWNMDITGTEIEVKSPWGHHLLKIKALGKFNIYNSLAVYSSLLASGYNPEK
VVQIMAELKAAPGRMEIVAQSPYVLVDYAHTPDALENVLITLKQIKKGRLWVVFGCGGDRDKTKRPIMGKAASMYADNIV
ITSDNPRSEDPEIILNEIACGMPASAPVSKLVNREEAIAYALRKAEHNDIILIAGKGHESYQQIGNTKHVFSDQEVVRRL
MNN
>Mature_483_residues
MKLTQLLEPWIKRDIIDGTVSDIKNDSRLVKEGDLFAAYPGAASDGRLYIEKAVASGAAAVVYEPNNLPQGITLPTAIPC
IAIPQLAEKLGEIAKRFFNNPSQFLGLTGVTGTNGKTTIAYQLAQAHDLLGQHSAYIGTIGQGKVDALKPLENTTPDALC
LQRLMHQYKEEGIKQVCMEVSSHALSQHRVDSLEFSQAIFTNLTLDHLDYHQTMQAYGAAKSRLFERSELQWAIINQDDD
CQDLMSNVLKTHVKKVTYGMHQNCDVKAIQWNMDITGTEIEVKSPWGHHLLKIKALGKFNIYNSLAVYSSLLASGYNPEK
VVQIMAELKAAPGRMEIVAQSPYVLVDYAHTPDALENVLITLKQIKKGRLWVVFGCGGDRDKTKRPIMGKAASMYADNIV
ITSDNPRSEDPEIILNEIACGMPASAPVSKLVNREEAIAYALRKAEHNDIILIAGKGHESYQQIGNTKHVFSDQEVVRRL
MNN

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=492, Percent_Identity=41.260162601626, Blast_Score=330, Evalue=9e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 53410; Mature: 53410

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTQLLEPWIKRDIIDGTVSDIKNDSRLVKEGDLFAAYPGAASDGRLYIEKAVASGAAA
CCHHHHHHHHHHHHHCCCHHHHCCCCCCEEECCCEEEECCCCCCCCEEEEEEHHCCCCEE
VVYEPNNLPQGITLPTAIPCIAIPQLAEKLGEIAKRFFNNPSQFLGLTGVTGTNGKTTIA
EEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHEEEECCCCCCCCEEEE
YQLAQAHDLLGQHSAYIGTIGQGKVDALKPLENTTPDALCLQRLMHQYKEEGIKQVCMEV
EHHHHHHHHHHCCCCEEEECCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SSHALSQHRVDSLEFSQAIFTNLTLDHLDYHQTMQAYGAAKSRLFERSELQWAIINQDDD
HHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCC
CQDLMSNVLKTHVKKVTYGMHQNCDVKAIQWNMDITGTEIEVKSPWGHHLLKIKALGKFN
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCEEEEECCCCCEEEEEEECCCCC
IYNSLAVYSSLLASGYNPEKVVQIMAELKAAPGRMEIVAQSPYVLVDYAHTPDALENVLI
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCCEEEEEECCCCHHHHHHHH
TLKQIKKGRLWVVFGCGGDRDKTKRPIMGKAASMYADNIVITSDNPRSEDPEIILNEIAC
HHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHHHCCCEEEECCCCCCCCHHHHHHHHHC
GMPASAPVSKLVNREEAIAYALRKAEHNDIILIAGKGHESYQQIGNTKHVFSDQEVVRRL
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHCCCCCCCCCHHHHHHHH
MNN
HCC
>Mature Secondary Structure
MKLTQLLEPWIKRDIIDGTVSDIKNDSRLVKEGDLFAAYPGAASDGRLYIEKAVASGAAA
CCHHHHHHHHHHHHHCCCHHHHCCCCCCEEECCCEEEECCCCCCCCEEEEEEHHCCCCEE
VVYEPNNLPQGITLPTAIPCIAIPQLAEKLGEIAKRFFNNPSQFLGLTGVTGTNGKTTIA
EEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHEEEECCCCCCCCEEEE
YQLAQAHDLLGQHSAYIGTIGQGKVDALKPLENTTPDALCLQRLMHQYKEEGIKQVCMEV
EHHHHHHHHHHCCCCEEEECCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SSHALSQHRVDSLEFSQAIFTNLTLDHLDYHQTMQAYGAAKSRLFERSELQWAIINQDDD
HHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCC
CQDLMSNVLKTHVKKVTYGMHQNCDVKAIQWNMDITGTEIEVKSPWGHHLLKIKALGKFN
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCEEEEECCCCCEEEEEEECCCCC
IYNSLAVYSSLLASGYNPEKVVQIMAELKAAPGRMEIVAQSPYVLVDYAHTPDALENVLI
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCCEEEEEECCCCHHHHHHHH
TLKQIKKGRLWVVFGCGGDRDKTKRPIMGKAASMYADNIVITSDNPRSEDPEIILNEIAC
HHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHHHCCCEEEECCCCCCCCHHHHHHHHHC
GMPASAPVSKLVNREEAIAYALRKAEHNDIILIAGKGHESYQQIGNTKHVFSDQEVVRRL
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHCCCCCCCCCHHHHHHHH
MNN
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]