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The map label for this gene is pcm [H]

Identifier: 54293684

GI number: 54293684

Start: 837788

End: 838438

Strand: Reverse

Name: pcm [H]

Synonym: lpl0737

Alternate gene names: 54293684

Gene position: 838438-837788 (Counterclockwise)

Preceding gene: 54293691

Following gene: 54293683

Centisome position: 25.06

GC content: 37.63

Gene sequence:

>651_bases
ATGAATCACAGTGCACGCATAAATATGATCAAACAACAGTTACGAACTGGTGATGTATTAAATGAATCCATCCTCGACTT
ATACGATGAGATTTTACGACATGAGTTTGTTCCAGAGCTTTTCTCTAATTTTGCTTATTCAGACATGCAAATTCCCTTGG
CACATGGCCAAAGGATGTTAACTCCATTGGAAGAAGGAACAATTATACAAGCCCTTGATCTTAAAGGCCATGAAACCGTG
CTGGAAGTAGGAACAGGAACCGGTTTTATGACTGCTTTACTCAGTAAATTATGTAAAAAAGTAATCAGTATTGACTATTA
TTCTGACTTTACTGCCAATGCAAAACGTAAACTTGAAGAGAATAATTGTAATAATGTTGAATTAATCACCGGAGATGCTT
GTCGTGGATGGTTGGAAAGTGCCCCATACGATGTTATGGTATTTACTGGTGCCATGGAAAAACTGACAGATACCCATAAA
CTACAAATTTTACCCGGTGGCAAGTTGTTTGCGATCCTTGGTAAATCCCCTGTAATGCAAGCTTATCTTTTTCAGTTGGA
TCATAATGCAATCTGGACAGAATCCATGCTTTTTGAAACGGATATACCTCCTCTGGTAGATCAATTGAAACCGAAAGAAT
TTGTTTTCTAG

Upstream 100 bases:

>100_bases
ATTTCACTTTCAAACATTAATGCTTAGACATAAGCTGAATTCTTGCTAAAATTGCTGCAAAGTTTTAACCTATCCAATGA
TTATTAAGACGGTAGAGCAA

Downstream 100 bases:

>100_bases
GGAAAACTCGAATGAGAAAATCTCTGTTTTGTTGGATTTTAACTTTAGGTGTTTCAACCCATGTGTTTGCAACAGACCTA
ATGGATATCTATCAACAAGC

Product: hypothetical protein

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT [H]

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MNHSARINMIKQQLRTGDVLNESILDLYDEILRHEFVPELFSNFAYSDMQIPLAHGQRMLTPLEEGTIIQALDLKGHETV
LEVGTGTGFMTALLSKLCKKVISIDYYSDFTANAKRKLEENNCNNVELITGDACRGWLESAPYDVMVFTGAMEKLTDTHK
LQILPGGKLFAILGKSPVMQAYLFQLDHNAIWTESMLFETDIPPLVDQLKPKEFVF

Sequences:

>Translated_216_residues
MNHSARINMIKQQLRTGDVLNESILDLYDEILRHEFVPELFSNFAYSDMQIPLAHGQRMLTPLEEGTIIQALDLKGHETV
LEVGTGTGFMTALLSKLCKKVISIDYYSDFTANAKRKLEENNCNNVELITGDACRGWLESAPYDVMVFTGAMEKLTDTHK
LQILPGGKLFAILGKSPVMQAYLFQLDHNAIWTESMLFETDIPPLVDQLKPKEFVF
>Mature_216_residues
MNHSARINMIKQQLRTGDVLNESILDLYDEILRHEFVPELFSNFAYSDMQIPLAHGQRMLTPLEEGTIIQALDLKGHETV
LEVGTGTGFMTALLSKLCKKVISIDYYSDFTANAKRKLEENNCNNVELITGDACRGWLESAPYDVMVFTGAMEKLTDTHK
LQILPGGKLFAILGKSPVMQAYLFQLDHNAIWTESMLFETDIPPLVDQLKPKEFVF

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789100, Length=172, Percent_Identity=29.6511627906977, Blast_Score=100, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 24431; Mature: 24431

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHSARINMIKQQLRTGDVLNESILDLYDEILRHEFVPELFSNFAYSDMQIPLAHGQRML
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHCCCHHC
TPLEEGTIIQALDLKGHETVLEVGTGTGFMTALLSKLCKKVISIDYYSDFTANAKRKLEE
CCCCCCCEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHC
NNCNNVELITGDACRGWLESAPYDVMVFTGAMEKLTDTHKLQILPGGKLFAILGKSPVMQ
CCCCCEEEEECHHHHHHHHCCCCEEEEEECHHHHHCCCCEEEEECCCCEEEEECCCHHHH
AYLFQLDHNAIWTESMLFETDIPPLVDQLKPKEFVF
HHHHHHCCCCEEHHHHHEECCCCHHHHHCCCHHCCC
>Mature Secondary Structure
MNHSARINMIKQQLRTGDVLNESILDLYDEILRHEFVPELFSNFAYSDMQIPLAHGQRML
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHCCCHHC
TPLEEGTIIQALDLKGHETVLEVGTGTGFMTALLSKLCKKVISIDYYSDFTANAKRKLEE
CCCCCCCEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHC
NNCNNVELITGDACRGWLESAPYDVMVFTGAMEKLTDTHKLQILPGGKLFAILGKSPVMQ
CCCCCEEEEECHHHHHHHHCCCCEEEEEECHHHHHCCCCEEEEECCCCEEEEECCCHHHH
AYLFQLDHNAIWTESMLFETDIPPLVDQLKPKEFVF
HHHHHHCCCCEEHHHHHEECCCCHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA