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The map label for this gene is ttcA [H]

Identifier: 54293682

GI number: 54293682

Start: 835541

End: 836392

Strand: Reverse

Name: ttcA [H]

Synonym: lpl0735

Alternate gene names: 54293682

Gene position: 836392-835541 (Counterclockwise)

Preceding gene: 54293683

Following gene: 54293681

Centisome position: 25.0

GC content: 37.68

Gene sequence:

>852_bases
ATGTCTTCCAATCCTTCTTCAGTTGAAAAAAAATTACTTCATTATACAGGTAAAGCCATAGCTGATTTTAATATGATCCA
GCGTGGTGACAAAGTCATGGTATGTCTGTCTGGCGGAAAAGATTCATTCACACTCTTAACAATTCTTAACCAGCTCAGGA
TCAAATCAGGAAATAAATTTGAAATTTTTGCGTTTACTCTGGATCAAGCCCAGCCAGGGTGGAATGATGCCTGTTTGCGT
CAATGGTTGGCTGAAAAATCCATTCCTCATGAAATACTAACCCGAGATACATACAGTATAGTGAAGGAAAAAATTCCTGA
AGGCAAAACCTACTGTTCTTTATGTTCTCGCTTGAGACGTGGAATCATCTATAGATATGCTGAAGAAAACGGATTTAATA
AAATAGCATTAGGTCACCATCGCGATGATCTTGTTCGTACATTGATGATGTCAATTCTATATAATGGCGATATACGTTCC
ATGCCACCTAAATTATTAAGCGATAATAAAAAGCACATAGTCATCCGGCCACTGTGTTATGTACAGGAAAAAGATATTAT
TACGTTTGCATCAGAACAAGCCTTTCCAATTATCCCATGCAATCTTTGTGGTTCTCAAGAAAATCTAATGCGCAAAAAAG
TTGCCTCACTCATTGATCAGCTTGCGATTGAAAACCCCAAAGTCCCCAGTAACATGCTTCATGCGCTGCAAAGTTTGAAG
CCAAGTCAATTGATGGATCAAAATTTTTGGAATTTTAAAAATCTGGAAGATGGGCTTGAAACCACGCAGTCAATCCAATG
TGAAGAGGTTTTTAACGCACAGGAATTTGAGCTTGAGGATGAAAAAATTTGA

Upstream 100 bases:

>100_bases
AATGATCTCGAACAGATTAACTCATGGTTAGCAACTACACGTGTCAATGGATTTTCACCCGTAGATAACAAAACCTCCAA
ATAACAAATTTAGGTTTTTT

Downstream 100 bases:

>100_bases
CTTATGAAAATTATTATCTAATATTTCTTTTCATTTTTGCAATATAATGAAATAATTGGCACGCAAAATACGCTTATTAT
TTTGACAAATGGAATTAAAT

Product: C32 tRNA thiolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MSSNPSSVEKKLLHYTGKAIADFNMIQRGDKVMVCLSGGKDSFTLLTILNQLRIKSGNKFEIFAFTLDQAQPGWNDACLR
QWLAEKSIPHEILTRDTYSIVKEKIPEGKTYCSLCSRLRRGIIYRYAEENGFNKIALGHHRDDLVRTLMMSILYNGDIRS
MPPKLLSDNKKHIVIRPLCYVQEKDIITFASEQAFPIIPCNLCGSQENLMRKKVASLIDQLAIENPKVPSNMLHALQSLK
PSQLMDQNFWNFKNLEDGLETTQSIQCEEVFNAQEFELEDEKI

Sequences:

>Translated_283_residues
MSSNPSSVEKKLLHYTGKAIADFNMIQRGDKVMVCLSGGKDSFTLLTILNQLRIKSGNKFEIFAFTLDQAQPGWNDACLR
QWLAEKSIPHEILTRDTYSIVKEKIPEGKTYCSLCSRLRRGIIYRYAEENGFNKIALGHHRDDLVRTLMMSILYNGDIRS
MPPKLLSDNKKHIVIRPLCYVQEKDIITFASEQAFPIIPCNLCGSQENLMRKKVASLIDQLAIENPKVPSNMLHALQSLK
PSQLMDQNFWNFKNLEDGLETTQSIQCEEVFNAQEFELEDEKI
>Mature_282_residues
SSNPSSVEKKLLHYTGKAIADFNMIQRGDKVMVCLSGGKDSFTLLTILNQLRIKSGNKFEIFAFTLDQAQPGWNDACLRQ
WLAEKSIPHEILTRDTYSIVKEKIPEGKTYCSLCSRLRRGIIYRYAEENGFNKIALGHHRDDLVRTLMMSILYNGDIRSM
PPKLLSDNKKHIVIRPLCYVQEKDIITFASEQAFPIIPCNLCGSQENLMRKKVASLIDQLAIENPKVPSNMLHALQSLKP
SQLMDQNFWNFKNLEDGLETTQSIQCEEVFNAQEFELEDEKI

Specific function: Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32) [H]

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TtcA family [H]

Homologues:

Organism=Escherichia coli, GI1787606, Length=280, Percent_Identity=49.6428571428571, Blast_Score=285, Evalue=3e-78,
Organism=Caenorhabditis elegans, GI193211090, Length=234, Percent_Identity=32.0512820512821, Blast_Score=117, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17540090, Length=183, Percent_Identity=28.4153005464481, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24651972, Length=239, Percent_Identity=26.3598326359833, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012089
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 32393; Mature: 32262

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSNPSSVEKKLLHYTGKAIADFNMIQRGDKVMVCLSGGKDSFTLLTILNQLRIKSGNKF
CCCCHHHHHHHHHHHHCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCEE
EIFAFTLDQAQPGWNDACLRQWLAEKSIPHEILTRDTYSIVKEKIPEGKTYCSLCSRLRR
EEEEEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
GIIYRYAEENGFNKIALGHHRDDLVRTLMMSILYNGDIRSMPPKLLSDNKKHIVIRPLCY
HHEEEECCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCEEEECCCHH
VQEKDIITFASEQAFPIIPCNLCGSQENLMRKKVASLIDQLAIENPKVPSNMLHALQSLK
CCCCCEEEEECCCCCCEEEHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
PSQLMDQNFWNFKNLEDGLETTQSIQCEEVFNAQEFELEDEKI
HHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHCCHHCCCCCCCC
>Mature Secondary Structure 
SSNPSSVEKKLLHYTGKAIADFNMIQRGDKVMVCLSGGKDSFTLLTILNQLRIKSGNKF
CCCHHHHHHHHHHHHCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCEE
EIFAFTLDQAQPGWNDACLRQWLAEKSIPHEILTRDTYSIVKEKIPEGKTYCSLCSRLRR
EEEEEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
GIIYRYAEENGFNKIALGHHRDDLVRTLMMSILYNGDIRSMPPKLLSDNKKHIVIRPLCY
HHEEEECCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCEEEECCCHH
VQEKDIITFASEQAFPIIPCNLCGSQENLMRKKVASLIDQLAIENPKVPSNMLHALQSLK
CCCCCEEEEECCCCCCEEEHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
PSQLMDQNFWNFKNLEDGLETTQSIQCEEVFNAQEFELEDEKI
HHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHCCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA