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The map label for this gene is rpoN [H]

Identifier: 54293469

GI number: 54293469

Start: 564866

End: 566260

Strand: Reverse

Name: rpoN [H]

Synonym: lpl0518

Alternate gene names: 54293469

Gene position: 566260-564866 (Counterclockwise)

Preceding gene: 54293470

Following gene: 54293468

Centisome position: 16.93

GC content: 36.42

Gene sequence:

>1395_bases
ATGAAACCGACATTGCAGATTGGTATTGGTCAACATCTCACGTTAACCCCACAACTGCAACAAGCCATAAGACTGCTGCA
ATTATCCACCTTTGATTTACAGCAAGAAATTCAACTCGTTGTTGAATCAAATCCTTTGCTTGAAGCCACTCCTGTTGAAG
AAAAAGAAGACGCCCAAATAAATACCAATCAGAACCATGATGAATATTCAGATTTTCAATGGTCTCAACTGTATAATAAC
TTAAACAAGCGCAATAATTTTGAAGAAAACGATTACAATTTTGATAATTTACACTGCACCACCACCAATTTACAAGATCA
TCTTAACTGGCAACTTGACCTGACGCCTATGAGCGATATTGACCGAGTCATTGCTACAACAATTATTGATGCGATAAACG
ATGACGGCTTTTTAACTATACCCGTTGCTGAAATTCACGCCAGCTTAAATAGCGATGATTTCCCTCTGGATATAGAAGAA
ATAGAAGCAGTGAGACACAGGCTTCAATTATTTGACCCCGTAGGATGCGCATCTGTTAATTTAGCAGAAACTCTCGTTTT
GCAATTGGAACAACTTCCACTGGAACCCAAGCTTTTGGCTACTGCGAAGAAAGTCATTCTTGAAGACATTGAGTTGCTAG
GGCAACATAACTACAGACAACTCATGAAAAATCATCAAATAAGTGAAAAAACAATTCATGAAATATTACAAGTCATTCAT
AAATTAAATCCAAAACCAGGAAGTTTGATCCATCAAGATAATATTGAATATGTTATTCCCGACTTAACTGTTAAAAAGAT
AGATAATCAATGGAAAGTTTTTTTGAATCAAGATATTCTTCCCAGGCTGAGTATCAATAGTCAATATGCCGCTTTAATCC
AACGTGCAAACAATAGTCTGGATAATCAATTCTTAAAGCATAATTTACAAGAAGCACGTTGGTTTTTAAAAAGTATTCAA
AGCCGTCAGGAAACACTTCTAAAAGTAGCAACCTGTATCATGGAATACCAACATGGATTTCTGGAATTTGGTGAAGAAGC
GATGAAACCATTAATATTGAATGATGTAGCTTCTGCCTTGGATATGCATGAATCAACGGTATCAAGAGTTACAACTCAAA
AATTTATTAACACTCCTAGAGGTGTTTTTGAGTTAAAGTACTTCTTCTCCAGCCATGTCAATACTGATAACGGAGGAGAA
TGCTCTTCCACCGCAATCCGAGCGGTAATAAAAAAATTAATTTCAGCAGAGAATAGAAAAAAACCTTTGAGTGATAGTAA
AATTGCCCAACTAATAGGTGAGCAAGGCATTAAAGTAGCAAGACGTACTGTTGCCAAATATCGCGAAGCAATGGGGATTC
CCCCATCCAATGAAAGAAAGGTTATTAGTAGTTAA

Upstream 100 bases:

>100_bases
CCATTACTTTTAAATTCAAATTAACGCCCGTAAGCACTGGATAATCCTTGTGGATCGTTTAAACTGATAGTTAAAGACAG
TGAAAAAAAAGAGGATTTGA

Downstream 100 bases:

>100_bases
TTAATCGGTCAACGAAAGGAGGTTTTATGCAAATCAACATTACTGGACATGGTATGGATGTCACTCCTGCACTAAGAGCA
TTCACTGAAGAAAAATTTGA

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MKPTLQIGIGQHLTLTPQLQQAIRLLQLSTFDLQQEIQLVVESNPLLEATPVEEKEDAQINTNQNHDEYSDFQWSQLYNN
LNKRNNFEENDYNFDNLHCTTTNLQDHLNWQLDLTPMSDIDRVIATTIIDAINDDGFLTIPVAEIHASLNSDDFPLDIEE
IEAVRHRLQLFDPVGCASVNLAETLVLQLEQLPLEPKLLATAKKVILEDIELLGQHNYRQLMKNHQISEKTIHEILQVIH
KLNPKPGSLIHQDNIEYVIPDLTVKKIDNQWKVFLNQDILPRLSINSQYAALIQRANNSLDNQFLKHNLQEARWFLKSIQ
SRQETLLKVATCIMEYQHGFLEFGEEAMKPLILNDVASALDMHESTVSRVTTQKFINTPRGVFELKYFFSSHVNTDNGGE
CSSTAIRAVIKKLISAENRKKPLSDSKIAQLIGEQGIKVARRTVAKYREAMGIPPSNERKVISS

Sequences:

>Translated_464_residues
MKPTLQIGIGQHLTLTPQLQQAIRLLQLSTFDLQQEIQLVVESNPLLEATPVEEKEDAQINTNQNHDEYSDFQWSQLYNN
LNKRNNFEENDYNFDNLHCTTTNLQDHLNWQLDLTPMSDIDRVIATTIIDAINDDGFLTIPVAEIHASLNSDDFPLDIEE
IEAVRHRLQLFDPVGCASVNLAETLVLQLEQLPLEPKLLATAKKVILEDIELLGQHNYRQLMKNHQISEKTIHEILQVIH
KLNPKPGSLIHQDNIEYVIPDLTVKKIDNQWKVFLNQDILPRLSINSQYAALIQRANNSLDNQFLKHNLQEARWFLKSIQ
SRQETLLKVATCIMEYQHGFLEFGEEAMKPLILNDVASALDMHESTVSRVTTQKFINTPRGVFELKYFFSSHVNTDNGGE
CSSTAIRAVIKKLISAENRKKPLSDSKIAQLIGEQGIKVARRTVAKYREAMGIPPSNERKVISS
>Mature_464_residues
MKPTLQIGIGQHLTLTPQLQQAIRLLQLSTFDLQQEIQLVVESNPLLEATPVEEKEDAQINTNQNHDEYSDFQWSQLYNN
LNKRNNFEENDYNFDNLHCTTTNLQDHLNWQLDLTPMSDIDRVIATTIIDAINDDGFLTIPVAEIHASLNSDDFPLDIEE
IEAVRHRLQLFDPVGCASVNLAETLVLQLEQLPLEPKLLATAKKVILEDIELLGQHNYRQLMKNHQISEKTIHEILQVIH
KLNPKPGSLIHQDNIEYVIPDLTVKKIDNQWKVFLNQDILPRLSINSQYAALIQRANNSLDNQFLKHNLQEARWFLKSIQ
SRQETLLKVATCIMEYQHGFLEFGEEAMKPLILNDVASALDMHESTVSRVTTQKFINTPRGVFELKYFFSSHVNTDNGGE
CSSTAIRAVIKKLISAENRKKPLSDSKIAQLIGEQGIKVARRTVAKYREAMGIPPSNERKVISS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the xylCAB operon and the xylS gene. The open complex (sigma-54 a

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=482, Percent_Identity=48.9626556016597, Blast_Score=468, Evalue=1e-133,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 52981; Mature: 52981

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPTLQIGIGQHLTLTPQLQQAIRLLQLSTFDLQQEIQLVVESNPLLEATPVEEKEDAQI
CCCCEEECCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCC
NTNQNHDEYSDFQWSQLYNNLNKRNNFEENDYNFDNLHCTTTNLQDHLNWQLDLTPMSDI
CCCCCCCHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCHHHHCCEEEECCCHHHH
DRVIATTIIDAINDDGFLTIPVAEIHASLNSDDFPLDIEEIEAVRHRLQLFDPVGCASVN
HHHHHHHHHHHHCCCCEEEEEHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCH
LAETLVLQLEQLPLEPKLLATAKKVILEDIELLGQHNYRQLMKNHQISEKTIHEILQVIH
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHH
KLNPKPGSLIHQDNIEYVIPDLTVKKIDNQWKVFLNQDILPRLSINSQYAALIQRANNSL
HCCCCCCCEEECCCCCEEECCCHHHHHCCHHHEEECCCCCCCCCCCHHHHHHHHHHCCHH
DNQFLKHNLQEARWFLKSIQSRQETLLKVATCIMEYQHGFLEFGEEAMKPLILNDVASAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DMHESTVSRVTTQKFINTPRGVFELKYFFSSHVNTDNGGECSSTAIRAVIKKLISAENRK
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCC
KPLSDSKIAQLIGEQGIKVARRTVAKYREAMGIPPSNERKVISS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MKPTLQIGIGQHLTLTPQLQQAIRLLQLSTFDLQQEIQLVVESNPLLEATPVEEKEDAQI
CCCCEEECCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCC
NTNQNHDEYSDFQWSQLYNNLNKRNNFEENDYNFDNLHCTTTNLQDHLNWQLDLTPMSDI
CCCCCCCHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCHHHHCCEEEECCCHHHH
DRVIATTIIDAINDDGFLTIPVAEIHASLNSDDFPLDIEEIEAVRHRLQLFDPVGCASVN
HHHHHHHHHHHHCCCCEEEEEHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCH
LAETLVLQLEQLPLEPKLLATAKKVILEDIELLGQHNYRQLMKNHQISEKTIHEILQVIH
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHH
KLNPKPGSLIHQDNIEYVIPDLTVKKIDNQWKVFLNQDILPRLSINSQYAALIQRANNSL
HCCCCCCCEEECCCCCEEECCCHHHHHCCHHHEEECCCCCCCCCCCHHHHHHHHHHCCHH
DNQFLKHNLQEARWFLKSIQSRQETLLKVATCIMEYQHGFLEFGEEAMKPLILNDVASAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DMHESTVSRVTTQKFINTPRGVFELKYFFSSHVNTDNGGECSSTAIRAVIKKLISAENRK
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCC
KPLSDSKIAQLIGEQGIKVARRTVAKYREAMGIPPSNERKVISS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2602128; 12534463 [H]