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The map label for this gene is vsr [H]

Identifier: 54293107

GI number: 54293107

Start: 180543

End: 180968

Strand: Direct

Name: vsr [H]

Synonym: lpl0146

Alternate gene names: 54293107

Gene position: 180543-180968 (Clockwise)

Preceding gene: 54293106

Following gene: 54293109

Centisome position: 5.4

GC content: 31.46

Gene sequence:

>426_bases
ATGACTGATAAAATTACCCCAGAGCATCGCTCAAGAAATATGGCTGCGATTAAAAATAAAAACACGCGTCCAGAGTTAGA
GGTTAGATCTTATCTATTTAAAAATGGTTTTAGATATAGACTTCACCGTAAAGATTTACCCGGTAAACCTGACTTAACTC
TAACAAAATATAAAACTGTTATATTTATAAATGGCTGTTTCTGGCATCGACATACAGGGTGTAAACTCGCTTATACTCCT
AAATCAAACTTCGAGTTTTGGGAACAAAAATTTCGCAAAAATGTAGCAAATGATTTAAAGAAACTAAAACAACTTAAGAT
GCTGGGATGGAAAGTCATTATCGTATGGGAGTGTGAAATAAAAGATAAAAACTATCATTGGATAGATAATGAAATAAAGA
ATGGCCTTGATAAAAATAAATATTAA

Upstream 100 bases:

>100_bases
TTCCTGTTATAAAAGCTATTGCACAAATTATGAAGCCTCATATTGAAATTCTTATGAAGAAGTCTGTATCTGCTGTTAAA
AAAGATGTTGCTTTAGAAAT

Downstream 100 bases:

>100_bases
CTGACAGCCTATTTCTTTCTCTCTAGTTTCTACTTCAGGATTTAGCTTACAACCTTCTATGATTGTGAATCAGTAGTTTC
TTAGATTTTGAAGAATTGAA

Product: hypothetical protein

Products: DNA [C]

Alternate protein names: V.XorII [H]

Number of amino acids: Translated: 141; Mature: 140

Protein sequence:

>141_residues
MTDKITPEHRSRNMAAIKNKNTRPELEVRSYLFKNGFRYRLHRKDLPGKPDLTLTKYKTVIFINGCFWHRHTGCKLAYTP
KSNFEFWEQKFRKNVANDLKKLKQLKMLGWKVIIVWECEIKDKNYHWIDNEIKNGLDKNKY

Sequences:

>Translated_141_residues
MTDKITPEHRSRNMAAIKNKNTRPELEVRSYLFKNGFRYRLHRKDLPGKPDLTLTKYKTVIFINGCFWHRHTGCKLAYTP
KSNFEFWEQKFRKNVANDLKKLKQLKMLGWKVIIVWECEIKDKNYHWIDNEIKNGLDKNKY
>Mature_140_residues
TDKITPEHRSRNMAAIKNKNTRPELEVRSYLFKNGFRYRLHRKDLPGKPDLTLTKYKTVIFINGCFWHRHTGCKLAYTPK
SNFEFWEQKFRKNVANDLKKLKQLKMLGWKVIIVWECEIKDKNYHWIDNEIKNGLDKNKY

Specific function: May nick XorII sequences that contain T:G mispairs resulting from m5C-deamination [H]

COG id: COG3727

COG function: function code L; DNA G:T-mismatch repair endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vsr family [H]

Homologues:

Organism=Escherichia coli, GI1788270, Length=124, Percent_Identity=40.3225806451613, Blast_Score=109, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004603
- InterPro:   IPR011335 [H]

Pfam domain/function: PF03852 Vsr [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 17091; Mature: 16960

Theoretical pI: Translated: 10.52; Mature: 10.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKITPEHRSRNMAAIKNKNTRPELEVRSYLFKNGFRYRLHRKDLPGKPDLTLTKYKTV
CCCCCCCHHHCCCEEEECCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEEEEEE
IFINGCFWHRHTGCKLAYTPKSNFEFWEQKFRKNVANDLKKLKQLKMLGWKVIIVWECEI
EEEECEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEE
KDKNYHWIDNEIKNGLDKNKY
CCCCCEECCHHHHCCCCCCCC
>Mature Secondary Structure 
TDKITPEHRSRNMAAIKNKNTRPELEVRSYLFKNGFRYRLHRKDLPGKPDLTLTKYKTV
CCCCCCHHHCCCEEEECCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEEEEEE
IFINGCFWHRHTGCKLAYTPKSNFEFWEQKFRKNVANDLKKLKQLKMLGWKVIIVWECEI
EEEECEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEE
KDKNYHWIDNEIKNGLDKNKY
CCCCCEECCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: DNA mismatch endonuclease vsr specifically recognizes a T-G mismatch in a special context related to the dcm methylation sequence and makes an incision 5' to the mismatched T residue [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8078464 [H]