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The map label for this gene is ecoRIIM [H]

Identifier: 54293106

GI number: 54293106

Start: 179293

End: 180546

Strand: Direct

Name: ecoRIIM [H]

Synonym: lpl0145

Alternate gene names: 54293106

Gene position: 179293-180546 (Clockwise)

Preceding gene: 54293104

Following gene: 54293107

Centisome position: 5.36

GC content: 35.09

Gene sequence:

>1254_bases
ATGAACACAATAACAAAGTTAAAAACCCTAAATGTATTACTTGGAGAGGAGCAACTTTCTAATTTTTTGGAGCTTTCCCC
CAAAACATTAAAAATCTGGATAAAGGATGGCAGCATTCCAAAACAATCCATTGTAGACGCAAAGTTTGATCAAATAATCG
AAAATATTAAGAATCCTAAAAGAAACCCAGTAAAGAGCTTTAGTTTTATTGATTTGTTTGCCGGCATCGGTGGTATCCGT
CTCGGGTTTGAAAGTATTGGTGGAGAATGTATTTTTACTTCTGAATGGGATAAGTATTCACAAATCACCTATCGTAAAAA
CTTTAGAAATGACCACCATCCAATTATAGGAGACATTACTAAAGTAGATGTTAATTTCATTCCAGAACACGATGTATTGC
TCGCTGGCTTTCCTTGCCAGCCATTTTCGCTCGCCGGAGTTTCTAAAAAGAACTCTCTTGGACGGGCACATGGATTTGAA
TGCAACACCCAGGGCACTTTATTCTTTGATGTAGAGCGCATAATAGCAGCAAAACAACCAAAAGCCTTTCTACTTGAAAA
TGTTAAAAATCTCAGAAGCCATGATAACGGAAAAACTTTTTCCATTATTATGGATACTCTACAACAAAAGTTAGGATACA
AAGTACATACTCTTGTAATAGACGCTAATGGATTTGTTCCTCAACATAGAGAAAGAATTTTTATTGTTGGTTTTAGGGAA
GATCTTCCATTCTCATGGAACGATCTCAGATTGCCTCCAAAAAACAGTGTATTACTCGAGTCTATACTACATCCTGAAAA
TGGCACTGAGATAGAAGAAAAGCCCTATACATTTGGAGAAAAAGCCGAAGTATCCTCTTGCTTTACTTTATCTGATAAAT
TGTGGAATTATTTGCAGGATTATAAGAAAAAGCATTCTGAAAAAGGAAATGGTTTTGGTTTTGGACTTGTTTCTGAAAAA
AATATAGCCCGTACTTTGTCAGCCCGGTATCATAAAGATGGTTCAGAAATATTAATTTATTGTGGAAAAGAAAAAAATCC
AAGAAGACTGACTCCCAGAGAATGTGCAAGACTTATGGGGTATCCCGATAGTTTTAAAATACCTGTATCAAATACTCAAG
CATACAGACAATTTGGTAACTCAGTGGTAGTTCCTGTTATAAAAGCTATTGCACAAATTATGAAGCCTCATATTGAAATT
CTTATGAAGAAGTCTGTATCTGCTGTTAAAAAAGATGTTGCTTTAGAAATATGA

Upstream 100 bases:

>100_bases
ATAAGATTATATAAAATAGTTCTATTTCTTCTTGTTTTAATTTCAATACTGTACTATCCTGGGAATTAAATTAGAAAAAA
ATTCTGGAATAACTCCTTTA

Downstream 100 bases:

>100_bases
CTGATAAAATTACCCCAGAGCATCGCTCAAGAAATATGGCTGCGATTAAAAATAAAAACACGCGTCCAGAGTTAGAGGTT
AGATCTTATCTATTTAAAAA

Product: hypothetical protein

Products: NA

Alternate protein names: M.EcoRII; Cytosine-specific methyltransferase EcoRII [H]

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MNTITKLKTLNVLLGEEQLSNFLELSPKTLKIWIKDGSIPKQSIVDAKFDQIIENIKNPKRNPVKSFSFIDLFAGIGGIR
LGFESIGGECIFTSEWDKYSQITYRKNFRNDHHPIIGDITKVDVNFIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFE
CNTQGTLFFDVERIIAAKQPKAFLLENVKNLRSHDNGKTFSIIMDTLQQKLGYKVHTLVIDANGFVPQHRERIFIVGFRE
DLPFSWNDLRLPPKNSVLLESILHPENGTEIEEKPYTFGEKAEVSSCFTLSDKLWNYLQDYKKKHSEKGNGFGFGLVSEK
NIARTLSARYHKDGSEILIYCGKEKNPRRLTPRECARLMGYPDSFKIPVSNTQAYRQFGNSVVVPVIKAIAQIMKPHIEI
LMKKSVSAVKKDVALEI

Sequences:

>Translated_417_residues
MNTITKLKTLNVLLGEEQLSNFLELSPKTLKIWIKDGSIPKQSIVDAKFDQIIENIKNPKRNPVKSFSFIDLFAGIGGIR
LGFESIGGECIFTSEWDKYSQITYRKNFRNDHHPIIGDITKVDVNFIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFE
CNTQGTLFFDVERIIAAKQPKAFLLENVKNLRSHDNGKTFSIIMDTLQQKLGYKVHTLVIDANGFVPQHRERIFIVGFRE
DLPFSWNDLRLPPKNSVLLESILHPENGTEIEEKPYTFGEKAEVSSCFTLSDKLWNYLQDYKKKHSEKGNGFGFGLVSEK
NIARTLSARYHKDGSEILIYCGKEKNPRRLTPRECARLMGYPDSFKIPVSNTQAYRQFGNSVVVPVIKAIAQIMKPHIEI
LMKKSVSAVKKDVALEI
>Mature_417_residues
MNTITKLKTLNVLLGEEQLSNFLELSPKTLKIWIKDGSIPKQSIVDAKFDQIIENIKNPKRNPVKSFSFIDLFAGIGGIR
LGFESIGGECIFTSEWDKYSQITYRKNFRNDHHPIIGDITKVDVNFIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFE
CNTQGTLFFDVERIIAAKQPKAFLLENVKNLRSHDNGKTFSIIMDTLQQKLGYKVHTLVIDANGFVPQHRERIFIVGFRE
DLPFSWNDLRLPPKNSVLLESILHPENGTEIEEKPYTFGEKAEVSSCFTLSDKLWNYLQDYKKKHSEKGNGFGFGLVSEK
NIARTLSARYHKDGSEILIYCGKEKNPRRLTPRECARLMGYPDSFKIPVSNTQAYRQFGNSVVVPVIKAIAQIMKPHIEI
LMKKSVSAVKKDVALEI

Specific function: This methylase recognizes the double-stranded sequence CCWGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the EcoRII endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788271, Length=397, Percent_Identity=52.3929471032746, Blast_Score=345, Evalue=3e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 47278; Mature: 47278

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: PS00094 C5_MTASE_1 ; PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTITKLKTLNVLLGEEQLSNFLELSPKTLKIWIKDGSIPKQSIVDAKFDQIIENIKNPK
CCCHHHHHHHHHEECHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCC
RNPVKSFSFIDLFAGIGGIRLGFESIGGECIFTSEWDKYSQITYRKNFRNDHHPIIGDIT
CCCCHHHHHHHHHHCCCCCEECHHHCCCEEEEECCCCCHHHHHHHHCCCCCCCCCCCCEE
KVDVNFIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFECNTQGTLFFDVERIIAAKQP
EEEEEECCCCCEEEECCCCCCCEECCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHCCCC
KAFLLENVKNLRSHDNGKTFSIIMDTLQQKLGYKVHTLVIDANGFVPQHRERIFIVGFRE
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCEEEEEEECC
DLPFSWNDLRLPPKNSVLLESILHPENGTEIEEKPYTFGEKAEVSSCFTLSDKLWNYLQD
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHEEHHHHHHHHHHH
YKKKHSEKGNGFGFGLVSEKNIARTLSARYHKDGSEILIYCGKEKNPRRLTPRECARLMG
HHHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHCC
YPDSFKIPVSNTQAYRQFGNSVVVPVIKAIAQIMKPHIEILMKKSVSAVKKDVALEI
CCCCCEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNTITKLKTLNVLLGEEQLSNFLELSPKTLKIWIKDGSIPKQSIVDAKFDQIIENIKNPK
CCCHHHHHHHHHEECHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCC
RNPVKSFSFIDLFAGIGGIRLGFESIGGECIFTSEWDKYSQITYRKNFRNDHHPIIGDIT
CCCCHHHHHHHHHHCCCCCEECHHHCCCEEEEECCCCCHHHHHHHHCCCCCCCCCCCCEE
KVDVNFIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFECNTQGTLFFDVERIIAAKQP
EEEEEECCCCCEEEECCCCCCCEECCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHCCCC
KAFLLENVKNLRSHDNGKTFSIIMDTLQQKLGYKVHTLVIDANGFVPQHRERIFIVGFRE
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCEEEEEEECC
DLPFSWNDLRLPPKNSVLLESILHPENGTEIEEKPYTFGEKAEVSSCFTLSDKLWNYLQD
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHEEHHHHHHHHHHH
YKKKHSEKGNGFGFGLVSEKNIARTLSARYHKDGSEILIYCGKEKNPRRLTPRECARLMG
HHHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHCC
YPDSFKIPVSNTQAYRQFGNSVVVPVIKAIAQIMKPHIEILMKKSVSAVKKDVALEI
CCCCCEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3029675 [H]