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The map label for this gene is pyrF

Identifier: 54297335

GI number: 54297335

Start: 1537887

End: 1538576

Strand: Direct

Name: pyrF

Synonym: lpp1380

Alternate gene names: 54297335

Gene position: 1537887-1538576 (Clockwise)

Preceding gene: 54297334

Following gene: 54297336

Centisome position: 43.89

GC content: 40.72

Gene sequence:

>690_bases
ATGACACCTAAATTAATCGTTGCGTTGGATTTTGATAATCAAGATAACGCTTTACAGTTGGTTGACAAACTGGATCCGAA
TCATTGCGCACTTAAGGTTGGAAGTGAGCTTTTTACTCTATTAGGGCCTCAGTTTGTAAAAGAATTGGTAAGAAGAGAGT
TTAAAGTATTTTTAGATTTAAAATTTCACGATATCCCTAATACAGTTGCTAAAGCATGCCACTCCGCTGCAGAATTAGGG
GTATGGATGATAAATGTTCATGCCATCGGTGGCTTAAGAATGTTGCAAGCTGCCAGAGAATCTTTAAAAACGTATGGTAA
AGACAGGCCTTTGTTGATTGCAGTGACTGTGCTGACCAGTTTTGAAGAAGGGGAGCTGGCCAGTGTTGGTATTAGTAATA
CATTGCCTGAGCAAGCAACCCATCTGGCTATGCTTGCCAGAGAGGCTGGGCTTGATGGTGTGGTAAGCTCTGCACATGAA
GTTAAAATTATAAAACAGAAGTGTGGTGAGAATTTTATTACCGTTACTCCTGGCATAAGACTGCCAAATAATCTAAAAGA
TGATCAATCGCGAGTAATGACTCCACAACAAGCCATCAGAGAAGGAAGTGATTTTCTGGTTATTGGAAGACCAATTACGC
AAGCGAGCAATCCTCATGAGGTTGTCTCAGCTCTATTGCGTGATCTTTGA

Upstream 100 bases:

>100_bases
CCTGCCTATGCACCCTTATTTACAAGAAGATGAGCAATTGAAAATAGTTGCAGCAGTTAGAAATGCTTTACTTGTGATGA
ATGAAGAGGCCATGGTATAA

Downstream 100 bases:

>100_bases
TGGCACATACTTTTAAAAAATTATACATATGGTATAATTTTGTTCATTTTTGAATTAGTTGTACCATTCCTATTTTATTC
CATAGAAATTTATGATAATT

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MTPKLIVALDFDNQDNALQLVDKLDPNHCALKVGSELFTLLGPQFVKELVRREFKVFLDLKFHDIPNTVAKACHSAAELG
VWMINVHAIGGLRMLQAARESLKTYGKDRPLLIAVTVLTSFEEGELASVGISNTLPEQATHLAMLAREAGLDGVVSSAHE
VKIIKQKCGENFITVTPGIRLPNNLKDDQSRVMTPQQAIREGSDFLVIGRPITQASNPHEVVSALLRDL

Sequences:

>Translated_229_residues
MTPKLIVALDFDNQDNALQLVDKLDPNHCALKVGSELFTLLGPQFVKELVRREFKVFLDLKFHDIPNTVAKACHSAAELG
VWMINVHAIGGLRMLQAARESLKTYGKDRPLLIAVTVLTSFEEGELASVGISNTLPEQATHLAMLAREAGLDGVVSSAHE
VKIIKQKCGENFITVTPGIRLPNNLKDDQSRVMTPQQAIREGSDFLVIGRPITQASNPHEVVSALLRDL
>Mature_228_residues
TPKLIVALDFDNQDNALQLVDKLDPNHCALKVGSELFTLLGPQFVKELVRREFKVFLDLKFHDIPNTVAKACHSAAELGV
WMINVHAIGGLRMLQAARESLKTYGKDRPLLIAVTVLTSFEEGELASVGISNTLPEQATHLAMLAREAGLDGVVSSAHEV
KIIKQKCGENFITVTPGIRLPNNLKDDQSRVMTPQQAIREGSDFLVIGRPITQASNPHEVVSALLRDL

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP)

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787537, Length=221, Percent_Identity=56.1085972850679, Blast_Score=252, Evalue=2e-68,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PYRF_LEGPA (Q5X5E0)

Other databases:

- EMBL:   CR628336
- RefSeq:   YP_123704.1
- ProteinModelPortal:   Q5X5E0
- SMR:   Q5X5E0
- STRING:   Q5X5E0
- GeneID:   3116697
- GenomeReviews:   CR628336_GR
- KEGG:   lpp:lpp1380
- LegioList:   lpp1380
- eggNOG:   COG0284
- HOGENOM:   HBG625253
- OMA:   TVHAYPQ
- ProtClustDB:   PRK00230
- BioCyc:   LPNE297246:LPP1380-MONOMER
- HAMAP:   MF_01200_B
- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00934
- TIGRFAMs:   TIGR01740

Pfam domain/function: PF00215 OMPdecase; SSF51366 RibP_bind_barrel

EC number: =4.1.1.23

Molecular weight: Translated: 25123; Mature: 24992

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00156 OMPDECASE

Important sites: ACT_SITE 61-61 BINDING 10-10 BINDING 32-32 BINDING 119-119 BINDING 180-180 BINDING 189-189 BINDING 209-209 BINDING 210-210

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPKLIVALDFDNQDNALQLVDKLDPNHCALKVGSELFTLLGPQFVKELVRREFKVFLDL
CCCCEEEEEECCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHEEE
KFHDIPNTVAKACHSAAELGVWMINVHAIGGLRMLQAARESLKTYGKDRPLLIAVTVLTS
EECCCCHHHHHHHHHHHHCCEEEEEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHC
FEEGELASVGISNTLPEQATHLAMLAREAGLDGVVSSAHEVKIIKQKCGENFITVTPGIR
CCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCC
LPNNLKDDQSRVMTPQQAIREGSDFLVIGRPITQASNPHEVVSALLRDL
CCCCCCCHHHHCCCHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TPKLIVALDFDNQDNALQLVDKLDPNHCALKVGSELFTLLGPQFVKELVRREFKVFLDL
CCCEEEEEECCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHEEE
KFHDIPNTVAKACHSAAELGVWMINVHAIGGLRMLQAARESLKTYGKDRPLLIAVTVLTS
EECCCCHHHHHHHHHHHHCCEEEEEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHC
FEEGELASVGISNTLPEQATHLAMLAREAGLDGVVSSAHEVKIIKQKCGENFITVTPGIR
CCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCC
LPNNLKDDQSRVMTPQQAIREGSDFLVIGRPITQASNPHEVVSALLRDL
CCCCCCCHHHHCCCHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA