Click here to switch to the map view.

The map label for this gene is cysS

Identifier: 54297226

GI number: 54297226

Start: 1408932

End: 1410302

Strand: Direct

Name: cysS

Synonym: lpp1271

Alternate gene names: 54297226

Gene position: 1408932-1410302 (Clockwise)

Preceding gene: 54297225

Following gene: 54297227

Centisome position: 40.21

GC content: 40.7

Gene sequence:

>1371_bases
ATGTTACATTTATATAATTCTTTGACCAGAAAAAAAGAACCATTTGTTTCCTTAAGGCCCGGAAAGATAGGTATGTATGT
TTGCGGAATTACTGTGTATGACCACTGCCACTTAGGGCATGCCCGTTCAATGGTTGCCTTTGATGTAATGGTTCGTTATC
TGCGCTCACAAGGATTCGATGTAACTTATGTACGCAATATAACTGACATAGATGATAAAATCATTGCAAGAGCCTCGGAG
AGAGGTGTATCTATTGATGAATTGACCGCACAATATATTGATGCAATGAATAATGATACTCACGCATTAAATATTTTGCC
ACCTGACCATGAGCCAAGGGCAACCGGGCACATTGAAACTATCATTCGCTTGATTCAACGTTTGCTTGAAAAAGGTAATG
CGTATGTCAGTGACAATGGAGATGTGTGCTATGAAGTAGATACCTTTCCCGAATATGGGAAATTATCGCATAAAGATATT
GAAGGCTTGGTTTCGGGTTCTCGCGTAGAGATTGTAAAAGAGAAACGTTCACCGCTTGATTTTGTGCTATGGAAAAAAGC
AAAACCGGGTGAACCCAGTTGGCCTTCACCGTGGGGGGAAGGGCGGCCTGGATGGCACATTGAGTGTTCAGCTATGGCTA
TGCATGAATTAGGTGAACAATTTGATATTCATGGAGGTGGATTGGATTTGCAATTCCCACATCATGAAAATGAAATTGCT
CAAAGTGAAGCGGCTACGGGTAAACCTTTTGCTAATTATTGGCTGCATGTCGGTATGTTGCAGGTCAATGGCGAAAAAAT
GGCAAAATCAATAGGGAACTTTTACACTATTGCCGATGTTTTAAAAGAGCATCATCCGGAAGTCATCCGCTATTTTCTAT
TAAGTAGTCATTATCGCAGTCCTTTGAATTATTCAGAAGAAAATTTGTTAAATGCCAAAAAAGCCCTTATCAGGCTTTAT
CAAGCAGTTAAAGATGTTCCTCCACAAACTGCAGATAGTAAACTGGATGAGTATTGGCAAGAGCAATTTAATCAGGCAAT
GAATGATGATTTCAATACTCCTGTTGCATTATCGGTTCTTTTCCAATTGGCTCATGAGGTAAACAAAAGTAATTCACCTG
CATTGGCTCATACCCTGAAAAACCTTGCTGGTATTCTTGGATTTTTACAAAAAGATCCCGAATCATTCCTGCAATCCGGC
TTGGCAGAGGAAGAAAAATTGGTTATAGAACAACTCATTGCAGAGCGTCTTCAGGCGCGAGCAGAACGCAATTGGGCAAA
GGCAGATCAGATTAGAGCTGATTTATTGAGCAAAGGCATAGAGCTGGAAGATGGTGCAACAGGAACAACATGGCGGAGGA
TTGCTGAATAA

Upstream 100 bases:

>100_bases
TTATTATTGCGTCAATTCAGTGGCTGAGGGCTGTGTTCAAGCCTTTCATCGGGTAGTGGATTTGAAGGACACTTGGGGTA
AAGTGAGTTGAGAGGCAGAC

Downstream 100 bases:

>100_bases
TTGTTCCTTCTATGCGCTTCTCTTGAATCAGAATGGACTTCTATCTCATACTTAATATAGGATATAGGTTGCAATTACCT
TTTCAGAAATTTTAAAACAG

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MLHLYNSLTRKKEPFVSLRPGKIGMYVCGITVYDHCHLGHARSMVAFDVMVRYLRSQGFDVTYVRNITDIDDKIIARASE
RGVSIDELTAQYIDAMNNDTHALNILPPDHEPRATGHIETIIRLIQRLLEKGNAYVSDNGDVCYEVDTFPEYGKLSHKDI
EGLVSGSRVEIVKEKRSPLDFVLWKKAKPGEPSWPSPWGEGRPGWHIECSAMAMHELGEQFDIHGGGLDLQFPHHENEIA
QSEAATGKPFANYWLHVGMLQVNGEKMAKSIGNFYTIADVLKEHHPEVIRYFLLSSHYRSPLNYSEENLLNAKKALIRLY
QAVKDVPPQTADSKLDEYWQEQFNQAMNDDFNTPVALSVLFQLAHEVNKSNSPALAHTLKNLAGILGFLQKDPESFLQSG
LAEEEKLVIEQLIAERLQARAERNWAKADQIRADLLSKGIELEDGATGTTWRRIAE

Sequences:

>Translated_456_residues
MLHLYNSLTRKKEPFVSLRPGKIGMYVCGITVYDHCHLGHARSMVAFDVMVRYLRSQGFDVTYVRNITDIDDKIIARASE
RGVSIDELTAQYIDAMNNDTHALNILPPDHEPRATGHIETIIRLIQRLLEKGNAYVSDNGDVCYEVDTFPEYGKLSHKDI
EGLVSGSRVEIVKEKRSPLDFVLWKKAKPGEPSWPSPWGEGRPGWHIECSAMAMHELGEQFDIHGGGLDLQFPHHENEIA
QSEAATGKPFANYWLHVGMLQVNGEKMAKSIGNFYTIADVLKEHHPEVIRYFLLSSHYRSPLNYSEENLLNAKKALIRLY
QAVKDVPPQTADSKLDEYWQEQFNQAMNDDFNTPVALSVLFQLAHEVNKSNSPALAHTLKNLAGILGFLQKDPESFLQSG
LAEEEKLVIEQLIAERLQARAERNWAKADQIRADLLSKGIELEDGATGTTWRRIAE
>Mature_456_residues
MLHLYNSLTRKKEPFVSLRPGKIGMYVCGITVYDHCHLGHARSMVAFDVMVRYLRSQGFDVTYVRNITDIDDKIIARASE
RGVSIDELTAQYIDAMNNDTHALNILPPDHEPRATGHIETIIRLIQRLLEKGNAYVSDNGDVCYEVDTFPEYGKLSHKDI
EGLVSGSRVEIVKEKRSPLDFVLWKKAKPGEPSWPSPWGEGRPGWHIECSAMAMHELGEQFDIHGGGLDLQFPHHENEIA
QSEAATGKPFANYWLHVGMLQVNGEKMAKSIGNFYTIADVLKEHHPEVIRYFLLSSHYRSPLNYSEENLLNAKKALIRLY
QAVKDVPPQTADSKLDEYWQEQFNQAMNDDFNTPVALSVLFQLAHEVNKSNSPALAHTLKNLAGILGFLQKDPESFLQSG
LAEEEKLVIEQLIAERLQARAERNWAKADQIRADLLSKGIELEDGATGTTWRRIAE

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=386, Percent_Identity=41.7098445595855, Blast_Score=278, Evalue=1e-74,
Organism=Homo sapiens, GI303304987, Length=249, Percent_Identity=44.9799196787149, Blast_Score=207, Evalue=2e-53,
Organism=Homo sapiens, GI62240992, Length=249, Percent_Identity=44.9799196787149, Blast_Score=207, Evalue=2e-53,
Organism=Homo sapiens, GI10835051, Length=249, Percent_Identity=44.9799196787149, Blast_Score=207, Evalue=2e-53,
Organism=Homo sapiens, GI21269877, Length=249, Percent_Identity=44.9799196787149, Blast_Score=207, Evalue=2e-53,
Organism=Escherichia coli, GI1786737, Length=461, Percent_Identity=56.1822125813449, Blast_Score=517, Evalue=1e-148,
Organism=Caenorhabditis elegans, GI17509795, Length=264, Percent_Identity=39.0151515151515, Blast_Score=188, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI17509797, Length=264, Percent_Identity=39.0151515151515, Blast_Score=188, Evalue=6e-48,
Organism=Saccharomyces cerevisiae, GI6324082, Length=339, Percent_Identity=35.3982300884956, Blast_Score=188, Evalue=1e-48,
Organism=Drosophila melanogaster, GI19922218, Length=501, Percent_Identity=34.1317365269461, Blast_Score=259, Evalue=3e-69,
Organism=Drosophila melanogaster, GI19922364, Length=250, Percent_Identity=43.2, Blast_Score=197, Evalue=1e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_LEGPA (Q5X5P9)

Other databases:

- EMBL:   CR628336
- RefSeq:   YP_123595.1
- ProteinModelPortal:   Q5X5P9
- SMR:   Q5X5P9
- STRING:   Q5X5P9
- GeneID:   3118289
- GenomeReviews:   CR628336_GR
- KEGG:   lpp:lpp1271
- LegioList:   lpp1271
- eggNOG:   COG0215
- HOGENOM:   HBG327651
- OMA:   VVKIYCC
- ProtClustDB:   PRK00260
- BioCyc:   LPNE297246:LPP1271-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 51571; Mature: 51571

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 269-269

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHLYNSLTRKKEPFVSLRPGKIGMYVCGITVYDHCHLGHARSMVAFDVMVRYLRSQGFD
CCCHHHHHHHCCCCCEEECCCCEEEEEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHCCCC
VTYVRNITDIDDKIIARASERGVSIDELTAQYIDAMNNDTHALNILPPDHEPRATGHIET
EEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHH
IIRLIQRLLEKGNAYVSDNGDVCYEVDTFPEYGKLSHKDIEGLVSGSRVEIVKEKRSPLD
HHHHHHHHHHCCCEEEECCCCEEEEECCCCCCCCCCHHHHHHHHCCCHHHHHHHCCCCCC
FVLWKKAKPGEPSWPSPWGEGRPGWHIECSAMAMHELGEQFDIHGGGLDLQFPHHENEIA
EEEECCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHCCHHEECCCCCEEEECCCCHHHHH
QSEAATGKPFANYWLHVGMLQVNGEKMAKSIGNFYTIADVLKEHHPEVIRYFLLSSHYRS
HHHCCCCCCHHHHHHEEEEEEECHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCC
PLNYSEENLLNAKKALIRLYQAVKDVPPQTADSKLDEYWQEQFNQAMNDDFNTPVALSVL
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
FQLAHEVNKSNSPALAHTLKNLAGILGFLQKDPESFLQSGLAEEEKLVIEQLIAERLQAR
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHH
AERNWAKADQIRADLLSKGIELEDGATGTTWRRIAE
HHHCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCC
>Mature Secondary Structure
MLHLYNSLTRKKEPFVSLRPGKIGMYVCGITVYDHCHLGHARSMVAFDVMVRYLRSQGFD
CCCHHHHHHHCCCCCEEECCCCEEEEEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHCCCC
VTYVRNITDIDDKIIARASERGVSIDELTAQYIDAMNNDTHALNILPPDHEPRATGHIET
EEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHH
IIRLIQRLLEKGNAYVSDNGDVCYEVDTFPEYGKLSHKDIEGLVSGSRVEIVKEKRSPLD
HHHHHHHHHHCCCEEEECCCCEEEEECCCCCCCCCCHHHHHHHHCCCHHHHHHHCCCCCC
FVLWKKAKPGEPSWPSPWGEGRPGWHIECSAMAMHELGEQFDIHGGGLDLQFPHHENEIA
EEEECCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHCCHHEECCCCCEEEECCCCHHHHH
QSEAATGKPFANYWLHVGMLQVNGEKMAKSIGNFYTIADVLKEHHPEVIRYFLLSSHYRS
HHHCCCCCCHHHHHHEEEEEEECHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCC
PLNYSEENLLNAKKALIRLYQAVKDVPPQTADSKLDEYWQEQFNQAMNDDFNTPVALSVL
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
FQLAHEVNKSNSPALAHTLKNLAGILGFLQKDPESFLQSGLAEEEKLVIEQLIAERLQAR
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHH
AERNWAKADQIRADLLSKGIELEDGATGTTWRRIAE
HHHCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA