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Identifier: 54296979

GI number: 54296979

Start: 1136515

End: 1138536

Strand: Direct

Name: lig

Synonym: lpp1020

Alternate gene names: 54296979

Gene position: 1136515-1138536 (Clockwise)

Preceding gene: 54296974

Following gene: 54296980

Centisome position: 32.44

GC content: 41.15

Gene sequence:

>2022_bases
ATGAATGATCAAGGAATTAAGGAATCGATAGAAACGCTTAAAGAGCAAATAAGAAAATACGATTATCACTATTATGTTTT
AGATGAACCTTTGGTTCCTGACGCGGAATATGATCGATGTTTCAAGGCATTGCAACAGTATGAAGAGCAATATCCGCAAT
TTTTATCGCCAGATTCCCCTACACAGAGAGTGAGCGGTACTCCTTCAGATGCTTTTATGCCGGTAGCCCATAAGCAACCC
ATGTTGTCTTTATCCAATGTGTTTACTATCGATGAATTAAAAGCATTCATTAAACGAGCAATTGAGAAACTGGATGAACC
AAATCAACAACTGGTATTTGCTTGCGAACCAAAGCTTGATGGGTTGGCTGTTAACATGACTTATGAGGGCGGGATCTTGA
CTCATGCCGCAACTCGTGGCGATGGTGCTGTAGGAGAAAACATCACGGCAAATATTAAGACTATTGCTTCAGTTCCATTA
AGGCTAAGGGTTAGTAACCCTCCAAAATTGATCGAAGTGCGGGGTGAAGTCTATATCCCCAAAGCCGATTTTGAAGCTTA
CAACGCAAGGGCTAGAGAACTCGGTGAAAAAACTTTTGCTAATCCGCGAAATGCTGCTGCAGGCAGTTTAAGACAATTAA
ATCCTGAAATTTCTGCCAGTCGTCCACTTGCTATTTATTGTTATAGTATAGGGGCTTGCGAGGATTATAAGTTACCTAAC
AGTCATTTGGAGCAATTGAATTTATTAAAAGAGTTTGGATTTAGAGTGTCTCCAGAAACGAGGAGGGCGATTGGAGTAGA
AGGCTGTTTAGATTATTACCAGTATATGTTAGCGAAACGGAATCAATTGCCATTTGAAATCGATGGGGTTGTTTATAAGA
TTGACAGTATCTCCTTGCAACAGCAATTAGGTTATGTTTCTCGTGCCCCAAGATTTGCTTGTGCCCATAAATTTCCCGCT
ACAGAAGAAATGACTCGTCTGATAGCCGTGGATTTCCAGGTAGGTAGAACGGGTGCTGTGACGCCGGTTGCACGTTTGGA
GCCAGTTAGTGTTGGTGGTGTTACAGTAAGTAACGCGACTTTGCATAATTTTGATGAAATTACACGAAAAGACATTCGTA
TTGGTGATACGGTTATTATTCGTCGTGCCGGTGATGTGATCCCTGAAGTAGTTTCTGTGATTTTGGAAAAGCGTCCCATT
AATGCCAGAAAGATTGAGCTTCCTAAAAATTGCCCTGTTTGTGGTTCTGAAGTCGTAAGGGAAGCGGATGAAGCAATTGC
TCGGTGTATCGGCGGTTTATATTGTAAAGCACAATTAAAAAGGATGATGTGGCATTTTGCTTCTCGAAAAGCGATGTATA
TTGAAGGACTTGGTAGTGTTTTAATTGATCAGTTAGTTGATGAGGGTATTGTCCATCATTTGGCGGATCTTTATGAACTC
GATTTGCAGACTTTAGCTAACCTGCCAAGGATGGGGGAGAAATCTGCAAAAAACTTATTATCCGCTTTGGAAAAAAGTAA
AAAAACGACTTTCAATCGCTTTCTTTATGCTTTGGGGATCAGAGAAATCGGTGAAGCTGGCGCAAGGGTTTTAGCGGAGC
ACTACTGTGATGTAGAGAGCTTGAAATCAGCAACGATTGAGGAATTAATGACTCTGAATGACATAGGTCCAGTAGCGGCT
TCTCATGTAGTCCATTTCTTTGCTCAAGCGCATAATCTTGAAGTGATTGACCGTCTTCTCGAGTTGGGTATTCATTGGCC
TAAGCCCGAAAAAATACAGGTTAATCAGCAAAATCCATTTTTTGGTAAAACAGTAGTTTTAACTGGAACTCTGAGTGCCA
TGGGGAGGGAAGAGGCAAAGGCAAAATTATTAGCCTTAGGTGCAAAAGTGAGTGGAAGTGTGTCTTCCAAAACGGATTAT
GTAATAGCAGGAAGTGAAGCCGGTTCAAAGCTGATTAAAGCGACAGAACTGGGAGTAGCGATTATAGAGGAAGACGAGTT
TTTAAAATGGGTTAATTCATGA

Upstream 100 bases:

>100_bases
GCTTCCAAAAAAAGATTTTGCCTTGAATGATTAAACCACTCATATTTGAAAGAAGTAAAAAAATGATAAACTGTTGTTTT
TATTTTGGGAATCTTCATTA

Downstream 100 bases:

>100_bases
GTAGCTAGATCTTTGTTATTTGGTTTATGATGGGTATCAATGAATCGGGTGAAGTAGGTATTTAAACCTGTAATAATGAC
TGATTGGCACGAGGGAAGTA

Product: DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 673; Mature: 673

Protein sequence:

>673_residues
MNDQGIKESIETLKEQIRKYDYHYYVLDEPLVPDAEYDRCFKALQQYEEQYPQFLSPDSPTQRVSGTPSDAFMPVAHKQP
MLSLSNVFTIDELKAFIKRAIEKLDEPNQQLVFACEPKLDGLAVNMTYEGGILTHAATRGDGAVGENITANIKTIASVPL
RLRVSNPPKLIEVRGEVYIPKADFEAYNARARELGEKTFANPRNAAAGSLRQLNPEISASRPLAIYCYSIGACEDYKLPN
SHLEQLNLLKEFGFRVSPETRRAIGVEGCLDYYQYMLAKRNQLPFEIDGVVYKIDSISLQQQLGYVSRAPRFACAHKFPA
TEEMTRLIAVDFQVGRTGAVTPVARLEPVSVGGVTVSNATLHNFDEITRKDIRIGDTVIIRRAGDVIPEVVSVILEKRPI
NARKIELPKNCPVCGSEVVREADEAIARCIGGLYCKAQLKRMMWHFASRKAMYIEGLGSVLIDQLVDEGIVHHLADLYEL
DLQTLANLPRMGEKSAKNLLSALEKSKKTTFNRFLYALGIREIGEAGARVLAEHYCDVESLKSATIEELMTLNDIGPVAA
SHVVHFFAQAHNLEVIDRLLELGIHWPKPEKIQVNQQNPFFGKTVVLTGTLSAMGREEAKAKLLALGAKVSGSVSSKTDY
VIAGSEAGSKLIKATELGVAIIEEDEFLKWVNS

Sequences:

>Translated_673_residues
MNDQGIKESIETLKEQIRKYDYHYYVLDEPLVPDAEYDRCFKALQQYEEQYPQFLSPDSPTQRVSGTPSDAFMPVAHKQP
MLSLSNVFTIDELKAFIKRAIEKLDEPNQQLVFACEPKLDGLAVNMTYEGGILTHAATRGDGAVGENITANIKTIASVPL
RLRVSNPPKLIEVRGEVYIPKADFEAYNARARELGEKTFANPRNAAAGSLRQLNPEISASRPLAIYCYSIGACEDYKLPN
SHLEQLNLLKEFGFRVSPETRRAIGVEGCLDYYQYMLAKRNQLPFEIDGVVYKIDSISLQQQLGYVSRAPRFACAHKFPA
TEEMTRLIAVDFQVGRTGAVTPVARLEPVSVGGVTVSNATLHNFDEITRKDIRIGDTVIIRRAGDVIPEVVSVILEKRPI
NARKIELPKNCPVCGSEVVREADEAIARCIGGLYCKAQLKRMMWHFASRKAMYIEGLGSVLIDQLVDEGIVHHLADLYEL
DLQTLANLPRMGEKSAKNLLSALEKSKKTTFNRFLYALGIREIGEAGARVLAEHYCDVESLKSATIEELMTLNDIGPVAA
SHVVHFFAQAHNLEVIDRLLELGIHWPKPEKIQVNQQNPFFGKTVVLTGTLSAMGREEAKAKLLALGAKVSGSVSSKTDY
VIAGSEAGSKLIKATELGVAIIEEDEFLKWVNS
>Mature_673_residues
MNDQGIKESIETLKEQIRKYDYHYYVLDEPLVPDAEYDRCFKALQQYEEQYPQFLSPDSPTQRVSGTPSDAFMPVAHKQP
MLSLSNVFTIDELKAFIKRAIEKLDEPNQQLVFACEPKLDGLAVNMTYEGGILTHAATRGDGAVGENITANIKTIASVPL
RLRVSNPPKLIEVRGEVYIPKADFEAYNARARELGEKTFANPRNAAAGSLRQLNPEISASRPLAIYCYSIGACEDYKLPN
SHLEQLNLLKEFGFRVSPETRRAIGVEGCLDYYQYMLAKRNQLPFEIDGVVYKIDSISLQQQLGYVSRAPRFACAHKFPA
TEEMTRLIAVDFQVGRTGAVTPVARLEPVSVGGVTVSNATLHNFDEITRKDIRIGDTVIIRRAGDVIPEVVSVILEKRPI
NARKIELPKNCPVCGSEVVREADEAIARCIGGLYCKAQLKRMMWHFASRKAMYIEGLGSVLIDQLVDEGIVHHLADLYEL
DLQTLANLPRMGEKSAKNLLSALEKSKKTTFNRFLYALGIREIGEAGARVLAEHYCDVESLKSATIEELMTLNDIGPVAA
SHVVHFFAQAHNLEVIDRLLELGIHWPKPEKIQVNQQNPFFGKTVVLTGTLSAMGREEAKAKLLALGAKVSGSVSSKTDY
VIAGSEAGSKLIKATELGVAIIEEDEFLKWVNS

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=671, Percent_Identity=53.6512667660209, Blast_Score=727, Evalue=0.0,
Organism=Escherichia coli, GI87082305, Length=526, Percent_Identity=22.4334600760456, Blast_Score=114, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_LEGPA (Q5X6E6)

Other databases:

- EMBL:   CR628336
- RefSeq:   YP_123348.1
- HSSP:   P26996
- ProteinModelPortal:   Q5X6E6
- STRING:   Q5X6E6
- GeneID:   3117317
- GenomeReviews:   CR628336_GR
- KEGG:   lpp:lpp1020
- LegioList:   lpp1020
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   CLSK2516597
- BioCyc:   LPNE297246:LPP1020-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 74745; Mature: 74745

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 118-118 BINDING 116-116 BINDING 139-139 BINDING 176-176 BINDING 293-293 BINDING 317-317

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDQGIKESIETLKEQIRKYDYHYYVLDEPLVPDAEYDRCFKALQQYEEQYPQFLSPDSP
CCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCHHHCCCCCC
TQRVSGTPSDAFMPVAHKQPMLSLSNVFTIDELKAFIKRAIEKLDEPNQQLVFACEPKLD
CHHCCCCCCCCCCCCCCCCCCHHHHHHEEHHHHHHHHHHHHHHHCCCCCEEEEEECCCCC
GLAVNMTYEGGILTHAATRGDGAVGENITANIKTIASVPLRLRVSNPPKLIEVRGEVYIP
CEEEEEEECCCEEEEECCCCCCCCCCCCEECHHHHCCCCEEEEECCCCCEEEECCCEEEC
KADFEAYNARARELGEKTFANPRNAAAGSLRQLNPEISASRPLAIYCYSIGACEDYKLPN
CCCCHHHHHHHHHHHHHHCCCCCCHHHCHHHHCCCCCCCCCCEEEEEEEECCCCCCCCCH
SHLEQLNLLKEFGFRVSPETRRAIGVEGCLDYYQYMLAKRNQLPFEIDGVVYKIDSISLQ
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCEEEEECCHHHH
QQLGYVSRAPRFACAHKFPATEEMTRLIAVDFQVGRTGAVTPVARLEPVSVGGVTVSNAT
HHHHHHHCCCCHHHHCCCCCHHHHHHHHHEEEEECCCCCCCCHHHCCCEEECCEEECCCC
LHNFDEITRKDIRIGDTVIIRRAGDVIPEVVSVILEKRPINARKIELPKNCPVCGSEVVR
HHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHH
EADEAIARCIGGLYCKAQLKRMMWHFASRKAMYIEGLGSVLIDQLVDEGIVHHLADLYEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLQTLANLPRMGEKSAKNLLSALEKSKKTTFNRFLYALGIREIGEAGARVLAEHYCDVES
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
LKSATIEELMTLNDIGPVAASHVVHFFAQAHNLEVIDRLLELGIHWPKPEKIQVNQQNPF
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCEEECCCCCC
FGKTVVLTGTLSAMGREEAKAKLLALGAKVSGSVSSKTDYVIAGSEAGSKLIKATELGVA
CCCEEEEEECHHHHCHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHCCEE
IIEEDEFLKWVNS
EECCHHHHHHHCC
>Mature Secondary Structure
MNDQGIKESIETLKEQIRKYDYHYYVLDEPLVPDAEYDRCFKALQQYEEQYPQFLSPDSP
CCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCHHHCCCCCC
TQRVSGTPSDAFMPVAHKQPMLSLSNVFTIDELKAFIKRAIEKLDEPNQQLVFACEPKLD
CHHCCCCCCCCCCCCCCCCCCHHHHHHEEHHHHHHHHHHHHHHHCCCCCEEEEEECCCCC
GLAVNMTYEGGILTHAATRGDGAVGENITANIKTIASVPLRLRVSNPPKLIEVRGEVYIP
CEEEEEEECCCEEEEECCCCCCCCCCCCEECHHHHCCCCEEEEECCCCCEEEECCCEEEC
KADFEAYNARARELGEKTFANPRNAAAGSLRQLNPEISASRPLAIYCYSIGACEDYKLPN
CCCCHHHHHHHHHHHHHHCCCCCCHHHCHHHHCCCCCCCCCCEEEEEEEECCCCCCCCCH
SHLEQLNLLKEFGFRVSPETRRAIGVEGCLDYYQYMLAKRNQLPFEIDGVVYKIDSISLQ
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCEEEEECCHHHH
QQLGYVSRAPRFACAHKFPATEEMTRLIAVDFQVGRTGAVTPVARLEPVSVGGVTVSNAT
HHHHHHHCCCCHHHHCCCCCHHHHHHHHHEEEEECCCCCCCCHHHCCCEEECCEEECCCC
LHNFDEITRKDIRIGDTVIIRRAGDVIPEVVSVILEKRPINARKIELPKNCPVCGSEVVR
HHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHH
EADEAIARCIGGLYCKAQLKRMMWHFASRKAMYIEGLGSVLIDQLVDEGIVHHLADLYEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLQTLANLPRMGEKSAKNLLSALEKSKKTTFNRFLYALGIREIGEAGARVLAEHYCDVES
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
LKSATIEELMTLNDIGPVAASHVVHFFAQAHNLEVIDRLLELGIHWPKPEKIQVNQQNPF
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCEEECCCCCC
FGKTVVLTGTLSAMGREEAKAKLLALGAKVSGSVSSKTDYVIAGSEAGSKLIKATELGVA
CCCEEEEEECHHHHCHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHCCEE
IIEEDEFLKWVNS
EECCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA