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The map label for this gene is kipI [H]

Identifier: 54027627

GI number: 54027627

Start: 5993050

End: 5993742

Strand: Reverse

Name: kipI [H]

Synonym: nfa56530

Alternate gene names: 54027627

Gene position: 5993742-5993050 (Counterclockwise)

Preceding gene: 54027628

Following gene: 54027626

Centisome position: 99.54

GC content: 73.16

Gene sequence:

>693_bases
GTGACGGGGACCGGTGCCGTGGCGAAGGACGCCGAACAGTGGTGGATCCGGGCCGCCGGTGACCGCGCCTGGCTGCTGTA
TCCCGGGAGTGCGATCACCGCGGCGCGCGTTGCCGCGCAACTGCGGGAGCGGCCGATCGAGGGTGTGCAGGATGTCCTGC
CCGCGGCGGAAACGGTGCTGGTGACGGTGCTGTCGCCCGCGCACGCCGAGCCGGTCCGGCGCGCGTTGGCGGCGCTGCTG
ACGCCGGGCGGCGCCGTTTCCCGTGAAACAGTCGGCGTGGCAAGTCTTTCCGACTCCGAAACGGCACCTGAGCCGGTCGA
GATCCCGGTGCGATACGACGGCGCCGACCTCGCGCACGTGGCCGAACTGCTCGGTACGAGCGTGCCCGCGGTGATCGAGG
CGCATACCGGCACCGTGTGGCGCTGCGGATTCGTCGGCTTCGCACCGGGATTCGGCTACCTGGAAGCACCGGACCGACGC
CTGACCGTCCCGCGTCGCGACCGCGCCCGCACCGCCATCCCGGCCGGCGCGGTCGCCCTGGCCGGCGGGTACTCGGCGGT
GTACCCACGCAGCACCCCGGGTGGCTGGCAGCTGATCGGGCACACCGACGCCGTGCTGTGGGACGTCGATCGCGATCCGC
CCGCGCTGATCCGCGCGGGCTCGGCGGTCCGGTTCGTGGCGGTCGACCGATGA

Upstream 100 bases:

>100_bases
GGCGACACCCCCGCCGCGGTCGAGATGGCGCGGCGGATCCGGGACGCGCTGGCCGCCGCCGGTGTGGTCGTCGAGGCGTT
CGTGTGAGCGCGCCGCGGGC

Downstream 100 bases:

>100_bases
TGCGGGTGCTGGCGGTCGGTCCGGCGGCCACGGTGCAGGACCTCGGCAGACCCGGGTGGTTCCATTCCGGGGTCGGCGTG
GCGGGCGCCGCCGATCGCGG

Product: hypothetical protein

Products: NA

Alternate protein names: Sporulation inhibitor kipI [H]

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MTGTGAVAKDAEQWWIRAAGDRAWLLYPGSAITAARVAAQLRERPIEGVQDVLPAAETVLVTVLSPAHAEPVRRALAALL
TPGGAVSRETVGVASLSDSETAPEPVEIPVRYDGADLAHVAELLGTSVPAVIEAHTGTVWRCGFVGFAPGFGYLEAPDRR
LTVPRRDRARTAIPAGAVALAGGYSAVYPRSTPGGWQLIGHTDAVLWDVDRDPPALIRAGSAVRFVAVDR

Sequences:

>Translated_230_residues
MTGTGAVAKDAEQWWIRAAGDRAWLLYPGSAITAARVAAQLRERPIEGVQDVLPAAETVLVTVLSPAHAEPVRRALAALL
TPGGAVSRETVGVASLSDSETAPEPVEIPVRYDGADLAHVAELLGTSVPAVIEAHTGTVWRCGFVGFAPGFGYLEAPDRR
LTVPRRDRARTAIPAGAVALAGGYSAVYPRSTPGGWQLIGHTDAVLWDVDRDPPALIRAGSAVRFVAVDR
>Mature_229_residues
TGTGAVAKDAEQWWIRAAGDRAWLLYPGSAITAARVAAQLRERPIEGVQDVLPAAETVLVTVLSPAHAEPVRRALAALLT
PGGAVSRETVGVASLSDSETAPEPVEIPVRYDGADLAHVAELLGTSVPAVIEAHTGTVWRCGFVGFAPGFGYLEAPDRRL
TVPRRDRARTAIPAGAVALAGGYSAVYPRSTPGGWQLIGHTDAVLWDVDRDPPALIRAGSAVRFVAVDR

Specific function: Is a potent inhibitor of the autophosphorylation reaction of kinase A and its reverse reaction, but does not inhibit phosphate transfer to the Spo0F response regulator once kinase A is phosphorylated. KipI is an inhibitor of the catalytic domain of kinase

COG id: COG2049

COG function: function code E; Allophanate hydrolase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786929, Length=124, Percent_Identity=49.1935483870968, Blast_Score=113, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003833
- InterPro:   IPR020899
- InterPro:   IPR010016
- InterPro:   IPR002130 [H]

Pfam domain/function: PF02682 AHS1 [H]

EC number: NA

Molecular weight: Translated: 24156; Mature: 24024

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGTGAVAKDAEQWWIRAAGDRAWLLYPGSAITAARVAAQLRERPIEGVQDVLPAAETVL
CCCCCCCHHCHHHHEEEECCCEEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHEEE
VTVLSPAHAEPVRRALAALLTPGGAVSRETVGVASLSDSETAPEPVEIPVRYDGADLAHV
EEEECCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCEEEEEEECCCCHHHH
AELLGTSVPAVIEAHTGTVWRCGFVGFAPGFGYLEAPDRRLTVPRRDRARTAIPAGAVAL
HHHHCCCCCEEEEECCCCEEEECEEECCCCCCCCCCCCCEECCCCCCCCCCCCCCCCEEE
AGGYSAVYPRSTPGGWQLIGHTDAVLWDVDRDPPALIRAGSAVRFVAVDR
ECCCEEECCCCCCCCEEEEECCCEEEEECCCCCHHHEECCCEEEEEEECC
>Mature Secondary Structure 
TGTGAVAKDAEQWWIRAAGDRAWLLYPGSAITAARVAAQLRERPIEGVQDVLPAAETVL
CCCCCCHHCHHHHEEEECCCEEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHEEE
VTVLSPAHAEPVRRALAALLTPGGAVSRETVGVASLSDSETAPEPVEIPVRYDGADLAHV
EEEECCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCEEEEEEECCCCHHHH
AELLGTSVPAVIEAHTGTVWRCGFVGFAPGFGYLEAPDRRLTVPRRDRARTAIPAGAVAL
HHHHCCCCCEEEEECCCCEEEECEEECCCCCCCCCCCCCEECCCCCCCCCCCCCCCCEEE
AGGYSAVYPRSTPGGWQLIGHTDAVLWDVDRDPPALIRAGSAVRFVAVDR
ECCCEEECCCCCCCCEEEEECCCEEEEECCCCCHHHEECCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377; 10568751 [H]