Click here to switch to the map view.

The map label for this gene is hetN [H]

Identifier: 54027500

GI number: 54027500

Start: 5853388

End: 5854080

Strand: Reverse

Name: hetN [H]

Synonym: nfa55260

Alternate gene names: 54027500

Gene position: 5854080-5853388 (Counterclockwise)

Preceding gene: 54027504

Following gene: 54027499

Centisome position: 97.22

GC content: 72.73

Gene sequence:

>693_bases
GTGACGAGCCTGCCGAAGCCCACCGCGTTGATCACCGGCGCCAGCCGCGGTCTCGGCGCCGCCATCGCGCGCGAACTCGC
GCCGACCCACGACCTGCTGCTCGGCGCCCGCACCGCCGACTCGCTGCGCGCGGTGCTCGACGAACTGCCCGGCGCGAGCG
CCTGGCCGGTGGAACTGACCGACTACGAAGCGGTCGCCGCCGCCACCGAACCCATCCGCCGCCTCGATGTGCTCGTGCAC
AACGCGGGCGTCGCCGACCTCGGCACCATCGCCGAATCCTCGGTGGGGCAATGGCGTACCACGCTGGAGGCCAACCTGAT
CGCGGTCGCCGAATTGACCAGGCTGCTGCTGCCCGCCCTGCGCGCGGCCGAAGGCCATGTGGTGCTGATCAATTCGGGTG
CCGGGCTGCGCGCCAACGCGGGCTGGGGCTCCTACGCCGCGAGCAAATTCGGCCTGCGCGCCTTCGGTGACGCGCTGCGA
CTGGAGGAACCGACGCTGCGGGTCACCTCGGTGCATCCGGGCCGCATCGACACCGACATGCAGCGCGCGATCGTCGCCGA
CGAGGGCCGCGAATACCGACCCGAGGAATTCCTGACCCCCGAGACCGTCGCCCGCGCCGTCCGCCACGCGGTGGAGACCC
CGCGCGACGGGCATCCCACCGAGATCGTGCTCCGCCCGACGCCGCCGCGCTGA

Upstream 100 bases:

>100_bases
CCCCGTCCCTGGCGATGGATTGGTTCCGGTATCCGCATCGTCGGGCGGCGCCGCGGTGCTACGCGATCGGCACACTACCG
TGGGGCCCATGACGACCGCT

Downstream 100 bases:

>100_bases
CTCGACACCGCCGGATAACCGGATGGTTGCGCGAAATCCGCCACCGGCTCACCTATCATCCGTACCGTGCCGCGGGACCG
GTTTTGAACCCCGGCGCGCC

Product: short chain dehydrogenase

Products: (3R)-hydroxyacyl-[acyl-carrier-protein]; NADP; NADPH; Proton; beta-ketoacyl-ACP [C]

Alternate protein names: NA

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MTSLPKPTALITGASRGLGAAIARELAPTHDLLLGARTADSLRAVLDELPGASAWPVELTDYEAVAAATEPIRRLDVLVH
NAGVADLGTIAESSVGQWRTTLEANLIAVAELTRLLLPALRAAEGHVVLINSGAGLRANAGWGSYAASKFGLRAFGDALR
LEEPTLRVTSVHPGRIDTDMQRAIVADEGREYRPEEFLTPETVARAVRHAVETPRDGHPTEIVLRPTPPR

Sequences:

>Translated_230_residues
MTSLPKPTALITGASRGLGAAIARELAPTHDLLLGARTADSLRAVLDELPGASAWPVELTDYEAVAAATEPIRRLDVLVH
NAGVADLGTIAESSVGQWRTTLEANLIAVAELTRLLLPALRAAEGHVVLINSGAGLRANAGWGSYAASKFGLRAFGDALR
LEEPTLRVTSVHPGRIDTDMQRAIVADEGREYRPEEFLTPETVARAVRHAVETPRDGHPTEIVLRPTPPR
>Mature_229_residues
TSLPKPTALITGASRGLGAAIARELAPTHDLLLGARTADSLRAVLDELPGASAWPVELTDYEAVAAATEPIRRLDVLVHN
AGVADLGTIAESSVGQWRTTLEANLIAVAELTRLLLPALRAAEGHVVLINSGAGLRANAGWGSYAASKFGLRAFGDALRL
EEPTLRVTSVHPGRIDTDMQRAIVADEGREYRPEEFLTPETVARAVRHAVETPRDGHPTEIVLRPTPPR

Specific function: May be involved in repressing heterocyst differentiation and may be essential for preventing all vegetative cells from differentiating [H]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI20149619, Length=206, Percent_Identity=33.9805825242718, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI109715829, Length=243, Percent_Identity=27.9835390946502, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI44680136, Length=185, Percent_Identity=30.8108108108108, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI44680133, Length=185, Percent_Identity=30.8108108108108, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI17738292, Length=185, Percent_Identity=30.8108108108108, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI7657478, Length=200, Percent_Identity=30, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI19743808, Length=199, Percent_Identity=31.6582914572864, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI7706318, Length=203, Percent_Identity=29.064039408867, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI150247226, Length=192, Percent_Identity=32.2916666666667, Blast_Score=66, Evalue=2e-11,
Organism=Homo sapiens, GI7705925, Length=183, Percent_Identity=29.5081967213115, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI1787335, Length=195, Percent_Identity=32.3076923076923, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1786701, Length=237, Percent_Identity=32.4894514767932, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI87082100, Length=234, Percent_Identity=30.3418803418803, Blast_Score=80, Evalue=8e-17,
Organism=Escherichia coli, GI1787820, Length=216, Percent_Identity=32.8703703703704, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1787526, Length=213, Percent_Identity=28.6384976525822, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1786812, Length=195, Percent_Identity=28.7179487179487, Blast_Score=60, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI71997402, Length=232, Percent_Identity=31.4655172413793, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17570069, Length=229, Percent_Identity=29.2576419213974, Blast_Score=84, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI71994600, Length=197, Percent_Identity=34.010152284264, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI193203117, Length=227, Percent_Identity=30.8370044052863, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI193203115, Length=227, Percent_Identity=30.8370044052863, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17560676, Length=226, Percent_Identity=28.7610619469027, Blast_Score=70, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17555706, Length=194, Percent_Identity=31.4432989690722, Blast_Score=70, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17508895, Length=231, Percent_Identity=29.004329004329, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17562906, Length=203, Percent_Identity=31.5270935960591, Blast_Score=64, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI71994604, Length=196, Percent_Identity=30.1020408163265, Blast_Score=63, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6322227, Length=215, Percent_Identity=31.6279069767442, Blast_Score=92, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6322226, Length=184, Percent_Identity=30.9782608695652, Blast_Score=82, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6323882, Length=252, Percent_Identity=30.1587301587302, Blast_Score=76, Evalue=4e-15,
Organism=Drosophila melanogaster, GI23397609, Length=199, Percent_Identity=37.6884422110553, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI28571526, Length=234, Percent_Identity=31.6239316239316, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21357041, Length=234, Percent_Identity=30.3418803418803, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24644339, Length=184, Percent_Identity=35.3260869565217, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI116007236, Length=242, Percent_Identity=27.6859504132231, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24641388, Length=242, Percent_Identity=30.5785123966942, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24641232, Length=242, Percent_Identity=30.1652892561983, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI28571387, Length=239, Percent_Identity=30.1255230125523, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI116007234, Length=242, Percent_Identity=29.7520661157025, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24648944, Length=238, Percent_Identity=29.8319327731092, Blast_Score=80, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24663911, Length=249, Percent_Identity=29.718875502008, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24648693, Length=242, Percent_Identity=29.7520661157025, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI21355889, Length=242, Percent_Identity=29.7520661157025, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI21358495, Length=207, Percent_Identity=28.9855072463768, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI281364494, Length=250, Percent_Identity=28.4, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24651139, Length=195, Percent_Identity=28.7179487179487, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI116007232, Length=174, Percent_Identity=32.183908045977, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24584752, Length=222, Percent_Identity=27.4774774774775, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100 [C]

Molecular weight: Translated: 24505; Mature: 24374

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSLPKPTALITGASRGLGAAIARELAPTHDLLLGARTADSLRAVLDELPGASAWPVELT
CCCCCCCCEEEECCCCCHHHHHHHHHCCHHHHHEECCCHHHHHHHHHHCCCCCCCCEEEC
DYEAVAAATEPIRRLDVLVHNAGVADLGTIAESSVGQWRTTLEANLIAVAELTRLLLPAL
CHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAAEGHVVLINSGAGLRANAGWGSYAASKFGLRAFGDALRLEEPTLRVTSVHPGRIDTDM
HCCCCCEEEEECCCCCEECCCCCCHHHHHHCHHHHHHHHCCCCCCEEEEEECCCCCCHHH
QRAIVADEGREYRPEEFLTPETVARAVRHAVETPRDGHPTEIVLRPTPPR
HHHHHHCCCCCCCCHHCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCC
>Mature Secondary Structure 
TSLPKPTALITGASRGLGAAIARELAPTHDLLLGARTADSLRAVLDELPGASAWPVELT
CCCCCCCEEEECCCCCHHHHHHHHHCCHHHHHEECCCHHHHHHHHHHCCCCCCCCEEEC
DYEAVAAATEPIRRLDVLVHNAGVADLGTIAESSVGQWRTTLEANLIAVAELTRLLLPAL
CHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAAEGHVVLINSGAGLRANAGWGSYAASKFGLRAFGDALRLEEPTLRVTSVHPGRIDTDM
HCCCCCEEEEECCCCCEECCCCCCHHHHHHCHHHHHHHHCCCCCCEEEEEECCCCCCHHH
QRAIVADEGREYRPEEFLTPETVARAVRHAVETPRDGHPTEIVLRPTPPR
HHHHHHCCCCCCCCHHCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Oxoacyl-[acyl-carrier-protein]; NADPH; D-3-hydroxy-acyl-ACP; NADP [C]

Specific reaction: Oxoacyl-[acyl-carrier-protein] + NADPH = (3R)-hydroxyacyl-[acyl-carrier-protein] + NADP+ D-3-hydroxy-acyl-ACP + NADP = NADPH + Proton + beta-ketoacyl-ACP [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8157596; 11759840 [H]