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The map label for this gene is echA22 [H]

Identifier: 54027418

GI number: 54027418

Start: 5760527

End: 5761291

Strand: Reverse

Name: echA22 [H]

Synonym: nfa54440

Alternate gene names: 54027418

Gene position: 5761291-5760527 (Counterclockwise)

Preceding gene: 54027419

Following gene: 54027417

Centisome position: 95.68

GC content: 71.37

Gene sequence:

>765_bases
ATGAGCGACACCCTGCGGGTCGAGCGCCGCGAGGCGGTCACCGTGCTCACCGTCGACCGTCCACGGGCGCGCAACGCCAT
CGACCTGGCGACCGCGCAGGCCATCGAGGCGGCCGTCGACGCCTTCGAAGCTGACGCCGACGCGCGTGTTCTCGTGCTCA
CCGGTTCGGGCGGAACATTCAGTGCTGGAATGGATCTCATCGCCGCGAGCAAGGGTGAGATGCCGATCACGCAACGGCGC
GGCCCACTCGGCATCACCGCCGCGCCGCCGTCCAAACCGATGATCTCGGCCGTCGAAGGGTATGCGCTGGCGGGCGGTTT
CGAACTCGCGATGGCCGGGGATCTCATCGTCGCCGCTCGCGACGCCCAGTTCGGGCTGCCCGAGGTCCGGCGCGGCCTGG
TCGCCGCCGCCGGTGGCGTGCTGCGGTTGACCCAGCGGCTGCCCCGGCCGATCGCGGCCGAACTCGCGCTCACCGGCGGG
CGGATCGGTGCCGAGCGGCTGTATCAGCTCGGGATGGTCAACCGGATCACCGAACCCGGTGAAGCGTTGGCGACGGCGCT
CGGGCTGGCCGCCGAGATCGCTTCCGCCGCACCACTGGCGGTCGCGGCGAGCAAGCGGATCATCGACGAATCGCCGGACT
GGTCGTCGGGCGAGGCGTTCGCCAAGCAGGGCGAGGTGGCGTTGCCCGCACTGGTGTCGAAGGACGCCGCTGAGGGCGCG
CTCGCGTTCGCCGAGAAGCGCGAACCCCGCTGGCAGGGACGCTGA

Upstream 100 bases:

>100_bases
ACCCCGGAATCATCGCCGCGTTCACCGAAACCGACCCGCTCGGCGCGAAGATCGATATCGTCGCCGCGGACCGGATCACC
CTGTCGGAAAGGACTGCCCG

Downstream 100 bases:

>100_bases
TCCGGTGCCCGAGACTCAGGCGCGGCGCCGGGCGCGCACCCGCGCGCGGCTGCTCGACGCGGCCGTGGCGAGCCTGGTCG
AAGTCGGCTACGCGGGCACC

Product: enoyl-CoA hydratase

Products: L-Carnitine.; L-carnitinyl-CoA [C]

Alternate protein names: Crotonobetainyl-CoA hydratase [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MSDTLRVERREAVTVLTVDRPRARNAIDLATAQAIEAAVDAFEADADARVLVLTGSGGTFSAGMDLIAASKGEMPITQRR
GPLGITAAPPSKPMISAVEGYALAGGFELAMAGDLIVAARDAQFGLPEVRRGLVAAAGGVLRLTQRLPRPIAAELALTGG
RIGAERLYQLGMVNRITEPGEALATALGLAAEIASAAPLAVAASKRIIDESPDWSSGEAFAKQGEVALPALVSKDAAEGA
LAFAEKREPRWQGR

Sequences:

>Translated_254_residues
MSDTLRVERREAVTVLTVDRPRARNAIDLATAQAIEAAVDAFEADADARVLVLTGSGGTFSAGMDLIAASKGEMPITQRR
GPLGITAAPPSKPMISAVEGYALAGGFELAMAGDLIVAARDAQFGLPEVRRGLVAAAGGVLRLTQRLPRPIAAELALTGG
RIGAERLYQLGMVNRITEPGEALATALGLAAEIASAAPLAVAASKRIIDESPDWSSGEAFAKQGEVALPALVSKDAAEGA
LAFAEKREPRWQGR
>Mature_253_residues
SDTLRVERREAVTVLTVDRPRARNAIDLATAQAIEAAVDAFEADADARVLVLTGSGGTFSAGMDLIAASKGEMPITQRRG
PLGITAAPPSKPMISAVEGYALAGGFELAMAGDLIVAARDAQFGLPEVRRGLVAAAGGVLRLTQRLPRPIAAELALTGGR
IGAERLYQLGMVNRITEPGEALATALGLAAEIASAAPLAVAASKRIIDESPDWSSGEAFAKQGEVALPALVSKDAAEGAL
AFAEKREPRWQGR

Specific function: Catalyzes the reversible dehydration of L-carnitinyl-CoA to crotonobetainyl-CoA [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=248, Percent_Identity=31.0483870967742, Blast_Score=118, Evalue=4e-27,
Organism=Homo sapiens, GI70995211, Length=271, Percent_Identity=30.9963099630996, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI31542718, Length=244, Percent_Identity=27.4590163934426, Blast_Score=88, Evalue=7e-18,
Organism=Homo sapiens, GI4502327, Length=269, Percent_Identity=30.8550185873606, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI150378541, Length=253, Percent_Identity=30.0395256916996, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI37594471, Length=187, Percent_Identity=28.3422459893048, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI37594469, Length=187, Percent_Identity=28.3422459893048, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI68989263, Length=165, Percent_Identity=32.7272727272727, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI20127408, Length=180, Percent_Identity=30.5555555555556, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI221142681, Length=263, Percent_Identity=43.3460076045627, Blast_Score=177, Evalue=4e-46,
Organism=Escherichia coli, GI1787659, Length=256, Percent_Identity=33.984375, Blast_Score=137, Evalue=6e-34,
Organism=Escherichia coli, GI87082183, Length=218, Percent_Identity=33.4862385321101, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1787660, Length=252, Percent_Identity=29.3650793650794, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1790281, Length=184, Percent_Identity=29.3478260869565, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788597, Length=259, Percent_Identity=24.7104247104247, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1788682, Length=213, Percent_Identity=31.924882629108, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17554946, Length=254, Percent_Identity=33.0708661417323, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI25145438, Length=243, Percent_Identity=32.0987654320988, Blast_Score=127, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17560910, Length=262, Percent_Identity=32.824427480916, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17536985, Length=265, Percent_Identity=24.9056603773585, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17558304, Length=200, Percent_Identity=30, Blast_Score=84, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17540714, Length=214, Percent_Identity=28.9719626168224, Blast_Score=84, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17508951, Length=205, Percent_Identity=32.6829268292683, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17508953, Length=205, Percent_Identity=32.6829268292683, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25144276, Length=205, Percent_Identity=32.6829268292683, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17534483, Length=257, Percent_Identity=24.124513618677, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17540306, Length=228, Percent_Identity=26.7543859649123, Blast_Score=77, Evalue=9e-15,
Organism=Drosophila melanogaster, GI20129971, Length=241, Percent_Identity=32.7800829875519, Blast_Score=124, Evalue=8e-29,
Organism=Drosophila melanogaster, GI24653477, Length=241, Percent_Identity=32.7800829875519, Blast_Score=124, Evalue=8e-29,
Organism=Drosophila melanogaster, GI21357171, Length=250, Percent_Identity=32.8, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24653139, Length=262, Percent_Identity=34.3511450381679, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24650670, Length=208, Percent_Identity=35.5769230769231, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI19922422, Length=257, Percent_Identity=25.6809338521401, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19920382, Length=225, Percent_Identity=29.3333333333333, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24583077, Length=187, Percent_Identity=29.4117647058824, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI24583079, Length=187, Percent_Identity=29.4117647058824, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI19921000, Length=187, Percent_Identity=29.4117647058824, Blast_Score=64, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022852
- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.- [C]

Molecular weight: Translated: 26291; Mature: 26160

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTLRVERREAVTVLTVDRPRARNAIDLATAQAIEAAVDAFEADADARVLVLTGSGGTF
CCCCCEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCC
SAGMDLIAASKGEMPITQRRGPLGITAAPPSKPMISAVEGYALAGGFELAMAGDLIVAAR
HHCCHHEECCCCCCCHHCCCCCEEEECCCCCCCHHHHHCCEEEECCEEEEEECCEEEEEC
DAQFGLPEVRRGLVAAAGGVLRLTQRLPRPIAAELALTGGRIGAERLYQLGMVNRITEPG
CCCCCCHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCH
EALATALGLAAEIASAAPLAVAASKRIIDESPDWSSGEAFAKQGEVALPALVSKDAAEGA
HHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHHCCCEEHHHHHCCCCHHHH
LAFAEKREPRWQGR
HHHHHHCCCCCCCC
>Mature Secondary Structure 
SDTLRVERREAVTVLTVDRPRARNAIDLATAQAIEAAVDAFEADADARVLVLTGSGGTF
CCCCEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCC
SAGMDLIAASKGEMPITQRRGPLGITAAPPSKPMISAVEGYALAGGFELAMAGDLIVAAR
HHCCHHEECCCCCCCHHCCCCCEEEECCCCCCCHHHHHCCEEEECCEEEEEECCEEEEEC
DAQFGLPEVRRGLVAAAGGVLRLTQRLPRPIAAELALTGGRIGAERLYQLGMVNRITEPG
CCCCCCHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCH
EALATALGLAAEIASAAPLAVAASKRIIDESPDWSSGEAFAKQGEVALPALVSKDAAEGA
HHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHHCCCEEHHHHHCCCCHHHH
LAFAEKREPRWQGR
HHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-Carnitine; D-carnitinyl-CoA [C]

Specific reaction: D-Carnitine = L-Carnitine. D-carnitinyl-CoA = L-carnitinyl-CoA [C]

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA