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The map label for this gene is metZ [H]

Identifier: 54027333

GI number: 54027333

Start: 5663886

End: 5665100

Strand: Reverse

Name: metZ [H]

Synonym: nfa53590

Alternate gene names: 54027333

Gene position: 5665100-5663886 (Counterclockwise)

Preceding gene: 54027334

Following gene: 54027328

Centisome position: 94.09

GC content: 70.21

Gene sequence:

>1215_bases
GTGATCACCGGCGGCGCGTTCGACAAGCCGCTGCCCGAGGGCGTCGGTCCCGCGACCCTCGGGGTGCGCGGCGGGCTGCG
CCGGTCCGGCTTCGAGGAGACCGCCGAGGCGCTCTACCTGACCTCCGGTTTCGTCTACGAGAGCGCCGAGGCCGCCGAGG
CCGCGTTCACCGGCGAGGTGGAGCACTTCGTCTACTCCCGGTACGGCAATCCGACCGTGGCGATGTTCGAGGAACGCATC
CGCCTGATGGACGGCGCCGAGGCCGCGTTCGCGACCGCCAGCGGTATGTCGGCGGTGTTCACCGCGCTCGGCGCGCTGCT
CGGCGCCGGCGACCGGCTGGTCGCGGCCCGCAGCCTGTTCGGGTCGTGTTTCGTGGTCTGCAACGAGATCCTGCCGCGCT
GGGGCGTGGAGACGGTGTTCGTCGACGGCGAGGACCTCGACCAGTGGGAGCGGGCACTGTCGGTGCCCACCGCCGCGGTG
TTCTTCGAGACGCCGGCCAACCCGATGCAGACCCTGGTCGACGTGCGCCGGGTCACCGAGCTCGCGCACGCCGCGGGCGC
GAAAGTGGTGCTGGACAACGTGTTCGCCACACCGCTGCTGCAGAAGGGCTTCGATCTCGGCGCCGACGTGGTCGTCTACT
CCGGCACCAAACACATCGACGGCCAGGGCCGGGTGCTCGGCGGTGCGATCCTCGGCGACCGCGAGTACATCGACGGCCCG
GTGAAGACCCTCATGCGCCACACCGGCCCGGCGCTGAGCCCGTTCAACGCCTGGACGCTGCTCAAGGGCCTCGAGACGAT
GCCGCTGCGCGTGCGGCACTCCACCGAGTCGGCGCTGCGGATCGCCCGCTTCCTCGAGTCGAACCCGGCGGTGAGCTGGG
TGAAATACCCGTTCCTCGAATCACATCCGCAGTACGACCTGGCCCGGGCTCAGATGTCCGGTGGCGGCACCGTGGTCACC
TTCGAGCTGAAGGCGGCCGAGGGCGAGGCCAAGAAGCGCGCGTTCGAGGTGCTCGACCGGCTGCGGATCATCGACATCTC
CAACAACCTCGGCGATGCCAAGACCCTCATCACCCACCCCGCCACCACCACCCATCGCGCGATGGGCCCCGAGGGCCGGG
CCGGTATCGGCCTGACCGACGGGGTGGTGCGCATCTCGGTGGGGCTGGAGGACGTCGACGACCTGCTCAGCGATCTCGAG
CACGCGCTGAGCTGA

Upstream 100 bases:

>100_bases
CGTCCTACAACGTGATCGAGGGCTTCGAGGGTTCGCTGGATGCCAACGGGCACCGCGGCGGCACCGGTTGGCGGGCCGAG
GGGCTGCCCTGGAGGCAGTC

Downstream 100 bases:

>100_bases
CCGGCTCATCCGGCCGGCCGCAGCAGTTCCGCGAGGTCGGCCGGGATCGGCATCGGCCGGCGCAGACCGACATTCGCGCG
CCCGGTGTTGCCCTCCGGAT

Product: O-succinylhomoserine sulfhydrylase

Products: Cystathionine; succinate; o-succinyl-L-homoserine; H2O [C]

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MITGGAFDKPLPEGVGPATLGVRGGLRRSGFEETAEALYLTSGFVYESAEAAEAAFTGEVEHFVYSRYGNPTVAMFEERI
RLMDGAEAAFATASGMSAVFTALGALLGAGDRLVAARSLFGSCFVVCNEILPRWGVETVFVDGEDLDQWERALSVPTAAV
FFETPANPMQTLVDVRRVTELAHAAGAKVVLDNVFATPLLQKGFDLGADVVVYSGTKHIDGQGRVLGGAILGDREYIDGP
VKTLMRHTGPALSPFNAWTLLKGLETMPLRVRHSTESALRIARFLESNPAVSWVKYPFLESHPQYDLARAQMSGGGTVVT
FELKAAEGEAKKRAFEVLDRLRIIDISNNLGDAKTLITHPATTTHRAMGPEGRAGIGLTDGVVRISVGLEDVDDLLSDLE
HALS

Sequences:

>Translated_404_residues
MITGGAFDKPLPEGVGPATLGVRGGLRRSGFEETAEALYLTSGFVYESAEAAEAAFTGEVEHFVYSRYGNPTVAMFEERI
RLMDGAEAAFATASGMSAVFTALGALLGAGDRLVAARSLFGSCFVVCNEILPRWGVETVFVDGEDLDQWERALSVPTAAV
FFETPANPMQTLVDVRRVTELAHAAGAKVVLDNVFATPLLQKGFDLGADVVVYSGTKHIDGQGRVLGGAILGDREYIDGP
VKTLMRHTGPALSPFNAWTLLKGLETMPLRVRHSTESALRIARFLESNPAVSWVKYPFLESHPQYDLARAQMSGGGTVVT
FELKAAEGEAKKRAFEVLDRLRIIDISNNLGDAKTLITHPATTTHRAMGPEGRAGIGLTDGVVRISVGLEDVDDLLSDLE
HALS
>Mature_404_residues
MITGGAFDKPLPEGVGPATLGVRGGLRRSGFEETAEALYLTSGFVYESAEAAEAAFTGEVEHFVYSRYGNPTVAMFEERI
RLMDGAEAAFATASGMSAVFTALGALLGAGDRLVAARSLFGSCFVVCNEILPRWGVETVFVDGEDLDQWERALSVPTAAV
FFETPANPMQTLVDVRRVTELAHAAGAKVVLDNVFATPLLQKGFDLGADVVVYSGTKHIDGQGRVLGGAILGDREYIDGP
VKTLMRHTGPALSPFNAWTLLKGLETMPLRVRHSTESALRIARFLESNPAVSWVKYPFLESHPQYDLARAQMSGGGTVVT
FELKAAEGEAKKRAFEVLDRLRIIDISNNLGDAKTLITHPATTTHRAMGPEGRAGIGLTDGVVRISVGLEDVDDLLSDLE
HALS

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=332, Percent_Identity=32.5301204819277, Blast_Score=194, Evalue=9e-50,
Organism=Homo sapiens, GI299473759, Length=332, Percent_Identity=30.1204819277108, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI25453487, Length=331, Percent_Identity=28.0966767371601, Blast_Score=144, Evalue=2e-34,
Organism=Escherichia coli, GI1790375, Length=387, Percent_Identity=31.0077519379845, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1789383, Length=366, Percent_Identity=24.8633879781421, Blast_Score=114, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17560056, Length=337, Percent_Identity=34.1246290801187, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17538055, Length=336, Percent_Identity=34.2261904761905, Blast_Score=197, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI71980708, Length=346, Percent_Identity=31.7919075144509, Blast_Score=177, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6323333, Length=410, Percent_Identity=32.1951219512195, Blast_Score=209, Evalue=5e-55,
Organism=Saccharomyces cerevisiae, GI6319307, Length=348, Percent_Identity=33.9080459770115, Blast_Score=184, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6321254, Length=407, Percent_Identity=25.3071253071253, Blast_Score=133, Evalue=6e-32,
Organism=Saccharomyces cerevisiae, GI6321904, Length=344, Percent_Identity=25.5813953488372, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321170, Length=267, Percent_Identity=27.3408239700375, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI19922554, Length=348, Percent_Identity=35.0574712643678, Blast_Score=219, Evalue=3e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.48 [C]

Molecular weight: Translated: 43313; Mature: 43313

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITGGAFDKPLPEGVGPATLGVRGGLRRSGFEETAEALYLTSGFVYESAEAAEAAFTGEV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHEEHCCCCEECCCHHHHHHHHHHH
EHFVYSRYGNPTVAMFEERIRLMDGAEAAFATASGMSAVFTALGALLGAGDRLVAARSLF
HHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHH
GSCFVVCNEILPRWGVETVFVDGEDLDQWERALSVPTAAVFFETPANPMQTLVDVRRVTE
HHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHH
LAHAAGAKVVLDNVFATPLLQKGFDLGADVVVYSGTKHIDGQGRVLGGAILGDREYIDGP
HHHHCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCEEECCCCEEEEEEEECCHHHCCHH
VKTLMRHTGPALSPFNAWTLLKGLETMPLRVRHSTESALRIARFLESNPAVSWVKYPFLE
HHHHHHHCCCCCCCCHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHCCCCCEEEECCCCC
SHPQYDLARAQMSGGGTVVTFELKAAEGEAKKRAFEVLDRLRIIDISNNLGDAKTLITHP
CCCCHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHEEEEEECCCCCCCHHEEECC
ATTTHRAMGPEGRAGIGLTDGVVRISVGLEDVDDLLSDLEHALS
CCCCHHCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MITGGAFDKPLPEGVGPATLGVRGGLRRSGFEETAEALYLTSGFVYESAEAAEAAFTGEV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHEEHCCCCEECCCHHHHHHHHHHH
EHFVYSRYGNPTVAMFEERIRLMDGAEAAFATASGMSAVFTALGALLGAGDRLVAARSLF
HHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHH
GSCFVVCNEILPRWGVETVFVDGEDLDQWERALSVPTAAVFFETPANPMQTLVDVRRVTE
HHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHH
LAHAAGAKVVLDNVFATPLLQKGFDLGADVVVYSGTKHIDGQGRVLGGAILGDREYIDGP
HHHHCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCEEECCCCEEEEEEEECCHHHCCHH
VKTLMRHTGPALSPFNAWTLLKGLETMPLRVRHSTESALRIARFLESNPAVSWVKYPFLE
HHHHHHHCCCCCCCCHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHCCCCCEEEECCCCC
SHPQYDLARAQMSGGGTVVTFELKAAEGEAKKRAFEVLDRLRIIDISNNLGDAKTLITHP
CCCCHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHEEEEEECCCCCCCHHEEECC
ATTTHRAMGPEGRAGIGLTDGVVRISVGLEDVDDLLSDLEHALS
CCCCHHCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 460 [C]

Specific activity: 10

Km value (mM): 0.33 {O-succinyl-L-homoserine}} [C]

Substrates: O-succinyl-L-homoserine; @CYST01.txt*L-cysteine!; 2-oxobutanoate; succinate; NH3 [C]

Specific reaction: O-succinyl-L-homoserine + @CYST01.txt*L-cysteine! = cystathionine + succinate 2-oxobutanoate + succinate + NH3 = o-succinyl-L-homoserine + H2O [C]

General reaction: Elimination; Gamma-replacement [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]