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The map label for this gene is aceA [H]

Identifier: 54027204

GI number: 54027204

Start: 5525226

End: 5526515

Strand: Reverse

Name: aceA [H]

Synonym: nfa52300

Alternate gene names: 54027204

Gene position: 5526515-5525226 (Counterclockwise)

Preceding gene: 54027207

Following gene: 54027203

Centisome position: 91.78

GC content: 67.29

Gene sequence:

>1290_bases
ATGTCGACCACCGGCACCCCGAAGACCGCTGAGGAGATCCAGAAGGATTGGGACACCAACCCTCGCTGGAAGGGCGTCAC
CCGTAACTACACCGCCGAGCAGGTGGTTGCGCTTCAGGGCAACGTCGTCGAGGAGCACACCCTCGCCCGTCGCGGCTCGG
AGATCCTGTGGGACCTCGTCAACAACGAGGACTACATCAACTCGCTGGGCGCCCTCACCGGCAACCAGGCCGTGCAGCAG
GTCCGCGCCGGCCTGAAGGCCATCTACCTGTCCGGCTGGCAGGTCGCCGGTGACGCGAACCTCTCGGGTCACACCTACCC
CGACCAGTCGCTGTACCCGGCCAACTCGGTTCCGGCCGTGGTCCGCCGCATCAACAACGCGCTGCTGCGCGCCGACGAGA
TCGCCAAGATCGAGGGCGACACCTCCGTCGAGAACTGGCTGGCCCCGATCGTGGCCGACGGTGAGGCGGGCTTCGGTGGC
GCGCTCAACGTCTACGAGCTGCAGAAGGCCATGATCGCCGCCGGTGTCGCCGGCTCGCACTGGGAAGACCAGCTGGCCTC
GGAGAAGAAGTGCGGCCACCTGGGCGGCAAGGTGCTCATCCCCACCCAGCAGCACATCCGCACCCTGACCTCCGCGCGTC
TGGCCGCCGACGTGGCCGGTGTGCCGACCGTCGTCATCGCCCGCACCGATGCCGAGGCCGCCACCCTGATCACCTCCGAC
GTGGACGAGCGCGACCGCCCGTTCATCACCGGTGAGCGCACCTCCGAGGGCTTCTACCAGGTCAAGAACGGCATCGAGCC
CTGCATCGCCCGCGCCAAGGCCTACGCGCCCTACGCGGACCTGATCTGGATGGAGACCGGCACCCCGGACCTCGAGCTGG
CCAAGAAGTTCTCCGAGGCCGTGCGCAGCGAGTTCCCGGACCAGCTGCTGGCCTACAACTGCTCGCCGTCGTTCAACTGG
TCGGCGCACCTGGACGACAGCACCATCGCCAAGTTCCAGAAGGAGCTGGGCGCGATGGGCTTCAAGTTCCAGTTCATCAC
CCTGGCGGGCTTCCACTCGCTCAACTACGGCATGTTCGACCTGGCCTACGGCTACGCCCGCGAGGGCATGACCGCCTTCG
TCGACCTGCAGAACCGCGAGTTCAAGGCCGCCGAGGAGCGTGGCTTCACCGCCATCAAGCACCAGCGCGAGGTCGGCGCG
GGCTACTTCGACGCCATCGCCACCACCGTCGACCCGAACACCTCGACGGCCGCGCTGAAGGGCTCCACCGAAGAGGGTCA
GTTCCACTGA

Upstream 100 bases:

>100_bases
ACACCCCGAAGGCATAGCAAGCCTGCAAAACGCCGCTCCAGCAGTTCGAGCGTCACGCTCGGCCTCGCGGCACCGACCGA
CCAAGGAAGTGGAGTCAGAG

Downstream 100 bases:

>100_bases
GCCGAAACGGCCGGTGACTACCGGGTTTTCCGCTAGTCCCCTACGCCGGTAGGGGTGTACCGCCCTCCCTCGCTGAACAG
CCCCGGCGAGGGAGGGCATC

Product: isocitrate lyase

Products: NA

Alternate protein names: ICL; Isocitrase; Isocitratase [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSTTGTPKTAEEIQKDWDTNPRWKGVTRNYTAEQVVALQGNVVEEHTLARRGSEILWDLVNNEDYINSLGALTGNQAVQQ
VRAGLKAIYLSGWQVAGDANLSGHTYPDQSLYPANSVPAVVRRINNALLRADEIAKIEGDTSVENWLAPIVADGEAGFGG
ALNVYELQKAMIAAGVAGSHWEDQLASEKKCGHLGGKVLIPTQQHIRTLTSARLAADVAGVPTVVIARTDAEAATLITSD
VDERDRPFITGERTSEGFYQVKNGIEPCIARAKAYAPYADLIWMETGTPDLELAKKFSEAVRSEFPDQLLAYNCSPSFNW
SAHLDDSTIAKFQKELGAMGFKFQFITLAGFHSLNYGMFDLAYGYAREGMTAFVDLQNREFKAAEERGFTAIKHQREVGA
GYFDAIATTVDPNTSTAALKGSTEEGQFH

Sequences:

>Translated_429_residues
MSTTGTPKTAEEIQKDWDTNPRWKGVTRNYTAEQVVALQGNVVEEHTLARRGSEILWDLVNNEDYINSLGALTGNQAVQQ
VRAGLKAIYLSGWQVAGDANLSGHTYPDQSLYPANSVPAVVRRINNALLRADEIAKIEGDTSVENWLAPIVADGEAGFGG
ALNVYELQKAMIAAGVAGSHWEDQLASEKKCGHLGGKVLIPTQQHIRTLTSARLAADVAGVPTVVIARTDAEAATLITSD
VDERDRPFITGERTSEGFYQVKNGIEPCIARAKAYAPYADLIWMETGTPDLELAKKFSEAVRSEFPDQLLAYNCSPSFNW
SAHLDDSTIAKFQKELGAMGFKFQFITLAGFHSLNYGMFDLAYGYAREGMTAFVDLQNREFKAAEERGFTAIKHQREVGA
GYFDAIATTVDPNTSTAALKGSTEEGQFH
>Mature_428_residues
STTGTPKTAEEIQKDWDTNPRWKGVTRNYTAEQVVALQGNVVEEHTLARRGSEILWDLVNNEDYINSLGALTGNQAVQQV
RAGLKAIYLSGWQVAGDANLSGHTYPDQSLYPANSVPAVVRRINNALLRADEIAKIEGDTSVENWLAPIVADGEAGFGGA
LNVYELQKAMIAAGVAGSHWEDQLASEKKCGHLGGKVLIPTQQHIRTLTSARLAADVAGVPTVVIARTDAEAATLITSDV
DERDRPFITGERTSEGFYQVKNGIEPCIARAKAYAPYADLIWMETGTPDLELAKKFSEAVRSEFPDQLLAYNCSPSFNWS
AHLDDSTIAKFQKELGAMGFKFQFITLAGFHSLNYGMFDLAYGYAREGMTAFVDLQNREFKAAEERGFTAIKHQREVGAG
YFDAIATTVDPNTSTAALKGSTEEGQFH

Specific function: Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle. May be involved in the assimilation of one-carbon compounds via the isocitrate lyase- positive serine pathway [H]

COG id: COG2224

COG function: function code C; Isocitrate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1790445, Length=428, Percent_Identity=61.9158878504673, Blast_Score=528, Evalue=1e-151,
Organism=Escherichia coli, GI1786525, Length=273, Percent_Identity=27.8388278388278, Blast_Score=80, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI71982926, Length=414, Percent_Identity=61.1111111111111, Blast_Score=519, Evalue=1e-148,
Organism=Caenorhabditis elegans, GI17561814, Length=414, Percent_Identity=61.1111111111111, Blast_Score=519, Evalue=1e-147,
Organism=Saccharomyces cerevisiae, GI6320908, Length=272, Percent_Identity=36.0294117647059, Blast_Score=162, Evalue=9e-41,
Organism=Saccharomyces cerevisiae, GI6325263, Length=251, Percent_Identity=35.8565737051793, Blast_Score=140, Evalue=5e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006254
- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.1 [H]

Molecular weight: Translated: 46809; Mature: 46677

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTGTPKTAEEIQKDWDTNPRWKGVTRNYTAEQVVALQGNVVEEHTLARRGSEILWDLV
CCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHEEEECCCCHHHHHHHHCCCHHHHHHH
NNEDYINSLGALTGNQAVQQVRAGLKAIYLSGWQVAGDANLSGHTYPDQSLYPANSVPAV
CCHHHHHHHHHCCHHHHHHHHHHHHHEEEECCEEEECCCCCCCCCCCCCCCCCCCCHHHH
VRRINNALLRADEIAKIEGDTSVENWLAPIVADGEAGFGGALNVYELQKAMIAAGVAGSH
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHCCCCCC
WEDQLASEKKCGHLGGKVLIPTQQHIRTLTSARLAADVAGVPTVVIARTDAEAATLITSD
HHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHEEEECC
VDERDRPFITGERTSEGFYQVKNGIEPCIARAKAYAPYADLIWMETGTPDLELAKKFSEA
CCCCCCCEEECCCCCCHHHHHHCCHHHHHHHHHHCCCHHHEEEEECCCCHHHHHHHHHHH
VRSEFPDQLLAYNCSPSFNWSAHLDDSTIAKFQKELGAMGFKFQFITLAGFHSLNYGMFD
HHHHCCCHHEEEECCCCCCCEECCCHHHHHHHHHHHCCCCEEEEEEEEECHHHCCCCHHH
LAYGYAREGMTAFVDLQNREFKAAEERGFTAIKHQREVGAGYFDAIATTVDPNTSTAALK
HHHHHHHCCCEEEEEECCCCCHHHHHCCCHHHHHHHHHCCCHHHHHHEECCCCCCCEEEE
GSTEEGQFH
CCCCCCCCC
>Mature Secondary Structure 
STTGTPKTAEEIQKDWDTNPRWKGVTRNYTAEQVVALQGNVVEEHTLARRGSEILWDLV
CCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHEEEECCCCHHHHHHHHCCCHHHHHHH
NNEDYINSLGALTGNQAVQQVRAGLKAIYLSGWQVAGDANLSGHTYPDQSLYPANSVPAV
CCHHHHHHHHHCCHHHHHHHHHHHHHEEEECCEEEECCCCCCCCCCCCCCCCCCCCHHHH
VRRINNALLRADEIAKIEGDTSVENWLAPIVADGEAGFGGALNVYELQKAMIAAGVAGSH
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHCCCCCC
WEDQLASEKKCGHLGGKVLIPTQQHIRTLTSARLAADVAGVPTVVIARTDAEAATLITSD
HHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHEEEECC
VDERDRPFITGERTSEGFYQVKNGIEPCIARAKAYAPYADLIWMETGTPDLELAKKFSEA
CCCCCCCEEECCCCCCHHHHHHCCHHHHHHHHHHCCCHHHEEEEECCCCHHHHHHHHHHH
VRSEFPDQLLAYNCSPSFNWSAHLDDSTIAKFQKELGAMGFKFQFITLAGFHSLNYGMFD
HHHHCCCHHEEEECCCCCCCEECCCHHHHHHHHHHHCCCCEEEEEEEEECHHHCCCCHHH
LAYGYAREGMTAFVDLQNREFKAAEERGFTAIKHQREVGAGYFDAIATTVDPNTSTAALK
HHHHHHHCCCEEEEEECCCCCHHHHHCCCHHHHHHHHHCCCHHHHHHEECCCCCCCEEEE
GSTEEGQFH
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8045408 [H]