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The map label for this gene is fadB5 [H]

Identifier: 54027203

GI number: 54027203

Start: 5524197

End: 5525066

Strand: Reverse

Name: fadB5 [H]

Synonym: nfa52290

Alternate gene names: 54027203

Gene position: 5525066-5524197 (Counterclockwise)

Preceding gene: 54027204

Following gene: 54027202

Centisome position: 91.76

GC content: 67.13

Gene sequence:

>870_bases
GTGAGCAGCGAGAAGATTCAGCGCGTCGGAGTCATCGGCGCCGGACAGATGGGCGCCGGAATCGCGGAAGTGTGCGCGCG
TGCACACGTCGACGTGCTCGTCTACGAACAGACCAGGGAACTGGCCGCCGCAGGCCGCGCCCGCATCCTGCGTTCGCTGG
ATCGTGGCGTCAGCAGCGGCAAGATCACCGAGCGCGAGCGCGAGCAGGCCGCCTGGCGGCTGCGCTTCACCTCCGACCTC
GGCGATTTCGCCGACCGCCAGCTGGTGGTCGAGGCCGTGCTGGAGAACGAGGAGGTCAAGACCTCGATCTTCGCCGAGCT
GGACAAGGTCGTCACCGACCCGAACGCGGTGCTGGCCTCCAACACCTCCTCCATTCCGATCATGAAGATCGCCGTCGCCA
CCGCCAATCCCGAGCGCGTGGTCGGCATGCACTTCTTCAACCCGGTGCCGGTGCTGCCGCTGGTCGAGCTGGTGACCACG
CTCAAGACCAGCGAAGCGGTGTCCGCGCGGGCCGAGGCGTTCGCCGGTGACGTGCTGGGCAAGCAGGTCGTGCGCTCGGC
CGACCGCTCCGGTTTCGTGGTCAACGCGCTGCTGGTGCCCTACCTGCTCTCGGCGATCCGGATGGTCGAATCCGGTTTCG
CCACCAAGGAAGACGTCGACAAGGCGATGGTGCTCGGCTGCGCCCACCCGATGGGCCCGCTCGCGCTGACCGACCTGGTC
GGCCTCGACACCGTCAAGTCCATCGCCGACTCCATGTACGCCGAGTTCAAGGAACCGCTGTACTCGGCCCCGCCGCTGCT
GATGCGCATGGTCGAGGCGGGCCTGCTCGGCAAGAAGACCGGCGCGGGCTTCTACCAGTACAACAAGTAA

Upstream 100 bases:

>100_bases
CCGCCCTCCCTCGCTGAACAGCCCCGGCGAGGGAGGGCATCCCTATACCTTGGACCAGAACCAGCCACCGCAAGCCAGAC
CAGAACAGCAGGAGCGGGAC

Downstream 100 bases:

>100_bases
CCACCTTCCGGTCCCCGGGGTTGCGCACACCCCGTTACTGCACGGCGCGGTAGCGGGGTGTTTCGCACCGGTGCTTCGCG
GTGCTCCCCGCGGCGGCCGA

Product: 3-hydroxybutyryl-CoA dehydrogenase

Products: NA

Alternate protein names: Beta-hydroxybutyryl-CoA dehydrogenase; BHBD [H]

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MSSEKIQRVGVIGAGQMGAGIAEVCARAHVDVLVYEQTRELAAAGRARILRSLDRGVSSGKITEREREQAAWRLRFTSDL
GDFADRQLVVEAVLENEEVKTSIFAELDKVVTDPNAVLASNTSSIPIMKIAVATANPERVVGMHFFNPVPVLPLVELVTT
LKTSEAVSARAEAFAGDVLGKQVVRSADRSGFVVNALLVPYLLSAIRMVESGFATKEDVDKAMVLGCAHPMGPLALTDLV
GLDTVKSIADSMYAEFKEPLYSAPPLLMRMVEAGLLGKKTGAGFYQYNK

Sequences:

>Translated_289_residues
MSSEKIQRVGVIGAGQMGAGIAEVCARAHVDVLVYEQTRELAAAGRARILRSLDRGVSSGKITEREREQAAWRLRFTSDL
GDFADRQLVVEAVLENEEVKTSIFAELDKVVTDPNAVLASNTSSIPIMKIAVATANPERVVGMHFFNPVPVLPLVELVTT
LKTSEAVSARAEAFAGDVLGKQVVRSADRSGFVVNALLVPYLLSAIRMVESGFATKEDVDKAMVLGCAHPMGPLALTDLV
GLDTVKSIADSMYAEFKEPLYSAPPLLMRMVEAGLLGKKTGAGFYQYNK
>Mature_288_residues
SSEKIQRVGVIGAGQMGAGIAEVCARAHVDVLVYEQTRELAAAGRARILRSLDRGVSSGKITEREREQAAWRLRFTSDLG
DFADRQLVVEAVLENEEVKTSIFAELDKVVTDPNAVLASNTSSIPIMKIAVATANPERVVGMHFFNPVPVLPLVELVTTL
KTSEAVSARAEAFAGDVLGKQVVRSADRSGFVVNALLVPYLLSAIRMVESGFATKEDVDKAMVLGCAHPMGPLALTDLVG
LDTVKSIADSMYAEFKEPLYSAPPLLMRMVEAGLLGKKTGAGFYQYNK

Specific function: Catalyzes the NAD-dependent dehydration of beta- hydroxybutyryl-CoA to acetoacetyl-CoA in vitro at pH 10. Also catalyzes the reverse reaction albeit in a lower pH range of 5.5- 6.5. The reverse reaction is able to use NADPH as well as NADH [H]

COG id: COG1250

COG function: function code I; 3-hydroxyacyl-CoA dehydrogenase

Gene ontology:

Cell location: Mitochondria or Peroxisomes [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyacyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI296179429, Length=290, Percent_Identity=42.7586206896552, Blast_Score=212, Evalue=3e-55,
Organism=Homo sapiens, GI296179427, Length=307, Percent_Identity=40.3908794788274, Blast_Score=202, Evalue=2e-52,
Organism=Homo sapiens, GI20127408, Length=285, Percent_Identity=33.6842105263158, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI261878539, Length=298, Percent_Identity=30.8724832214765, Blast_Score=122, Evalue=3e-28,
Organism=Homo sapiens, GI68989263, Length=298, Percent_Identity=30.8724832214765, Blast_Score=122, Evalue=3e-28,
Organism=Escherichia coli, GI1787661, Length=285, Percent_Identity=41.0526315789474, Blast_Score=181, Evalue=7e-47,
Organism=Escherichia coli, GI1788682, Length=235, Percent_Identity=36.1702127659575, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1790281, Length=246, Percent_Identity=34.9593495934959, Blast_Score=129, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17549919, Length=291, Percent_Identity=39.5189003436426, Blast_Score=205, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI17553560, Length=292, Percent_Identity=37.3287671232877, Blast_Score=199, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI17563036, Length=294, Percent_Identity=38.7755102040816, Blast_Score=185, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17508951, Length=283, Percent_Identity=33.5689045936396, Blast_Score=137, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI25144276, Length=283, Percent_Identity=33.5689045936396, Blast_Score=137, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI17508953, Length=283, Percent_Identity=33.5689045936396, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17558304, Length=283, Percent_Identity=32.1554770318021, Blast_Score=133, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI71985923, Length=285, Percent_Identity=29.4736842105263, Blast_Score=117, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI71985930, Length=289, Percent_Identity=28.0276816608997, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI115533614, Length=202, Percent_Identity=30.6930693069307, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI193203623, Length=202, Percent_Identity=30.6930693069307, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19921000, Length=284, Percent_Identity=36.6197183098592, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24583077, Length=284, Percent_Identity=36.6197183098592, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24583079, Length=284, Percent_Identity=36.6197183098592, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI45549573, Length=236, Percent_Identity=27.1186440677966, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022694
- InterPro:   IPR006176
- InterPro:   IPR006108
- InterPro:   IPR008927
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00725 3HCDH; PF02737 3HCDH_N [H]

EC number: =1.1.1.157 [H]

Molecular weight: Translated: 31098; Mature: 30967

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSEKIQRVGVIGAGQMGAGIAEVCARAHVDVLVYEQTRELAAAGRARILRSLDRGVSSG
CCCHHHHHHCCEECCCCCCCHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHCCCCCC
KITEREREQAAWRLRFTSDLGDFADRQLVVEAVLENEEVKTSIFAELDKVVTDPNAVLAS
CCCHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEC
NTSSIPIMKIAVATANPERVVGMHFFNPVPVLPLVELVTTLKTSEAVSARAEAFAGDVLG
CCCCCCEEEEEEEECCCCCEEEEHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQVVRSADRSGFVVNALLVPYLLSAIRMVESGFATKEDVDKAMVLGCAHPMGPLALTDLV
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
GLDTVKSIADSMYAEFKEPLYSAPPLLMRMVEAGLLGKKTGAGFYQYNK
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SSEKIQRVGVIGAGQMGAGIAEVCARAHVDVLVYEQTRELAAAGRARILRSLDRGVSSG
CCHHHHHHCCEECCCCCCCHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHCCCCCC
KITEREREQAAWRLRFTSDLGDFADRQLVVEAVLENEEVKTSIFAELDKVVTDPNAVLAS
CCCHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEC
NTSSIPIMKIAVATANPERVVGMHFFNPVPVLPLVELVTTLKTSEAVSARAEAFAGDVLG
CCCCCCEEEEEEEECCCCCEEEEHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQVVRSADRSGFVVNALLVPYLLSAIRMVESGFATKEDVDKAMVLGCAHPMGPLALTDLV
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
GLDTVKSIADSMYAEFKEPLYSAPPLLMRMVEAGLLGKKTGAGFYQYNK
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]