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The map label for this gene is thcD [H]

Identifier: 54027045

GI number: 54027045

Start: 5370325

End: 5371548

Strand: Reverse

Name: thcD [H]

Synonym: nfa50710

Alternate gene names: 54027045

Gene position: 5371548-5370325 (Counterclockwise)

Preceding gene: 54027048

Following gene: 54027044

Centisome position: 89.21

GC content: 74.59

Gene sequence:

>1224_bases
ATGTCGCCGAGGGAAGGGACCACCGCAATGAGCGCTCCGATCGTCATCGTGGGAGCCGGGCTGGCCGGACTGCGGACCGC
CGAGGAACTGCGCCGCGCCGGGTACGAGGGCGACCTCGTGCTGCTGGGCGACGAGGCGCGCCTGCCCTACGACCGCCCGC
CGCTGTCGAAGCAGTTCGTGCGCGGCGAGACCGACGACACCACGTTGCGCCCGGCCGAGTTCTTCACCGACAAGCGCATC
GAGCTGCGGCTGGGCACCACCGCGACCGGAGTGGACACCGCCACCCGCCGGGTCCTGCTCGCGGACGGCTCCGCGCTCGC
CTACGACCATCTCGTCATCGCGACCGGCCTGCGGCCCCGCACGCTTCCCGGGCTGCCGACGCCGGCCGGCGTGCACGTGT
TGCGCGACCATGCCGACGCCACCGCGTTGCGCGACGAATCAGCTTCCGCCACAGCGGCTCTGGTGATCGGCGCGGGCTTC
ATCGGCTGCGAGGTGGCGGCCAGCTTCCGGGCGCGCGGGCTGGACGTGGTGCTGGTCGAGCCGCAGCCGACGCCGTTGGC
CTCGGTGCTCGGCGAACAGGTCGGCGGGCTGGTCGCGCGCATGCACCGCGCCGAGGGGGTCGACCTGCGCTGCGGCACCG
GGGTGCGGACCCTGCTGTCCGACGACCGGGGCCGGGTGCGTGGGGCGCTGCTCTCCGACGGCGCGGAGGTGCGCGCCGAC
CTGGTCGTCCTGGGGGTGGGCTCGCGGCCCGCCGTCGAGTGGCTGGCCGATTCCGGTATCGCGCTGGCCGAGCAGGCGGC
CGGCGGTGGCGTACTGGCCGACGAGGTGGGCCGCACGTCGGTCGAGCGGGTCTGGGCGGTCGGCGACGTGGCGGCCTGGC
GGCACGAGACCGGCGCACAGCAGCGGGTCGAGCACTGGACCAACGCGGGGGAGCAGGCCAAGCTGGTGGCCTGTGCGCTG
CTCGGCGCCGAACCGCCGACGGCCGCGCGGGTGCCGTACTTCTGGAGCGATCAGTACGACGTGAAGATCCAGGCGCTCGG
CACCCCGAGCGCCGACGACGACGTCTCGGTGGCGGCCGACGACGGCCGCAAGTTCCTCGCGTACTACTCGCGCGCGGGCG
CCCTCACCGCGGTGGTCGGCGCGGGCATGACGGCGCAGGTCATGAAGGCCCGCGCCAAGGTGGCCGCGGGCGCTCCGGTG
GCCGATCTGCTGGCCACCACCTGA

Upstream 100 bases:

>100_bases
ATGGATACGATTGCGCGGCGAAGTGCGATACGCCCGCACCGGGTGCATCGCGCTCGGAAAGGAGCGGTCCCACGATCGAC
CAAGCGCTTGCTAGGGTGAG

Downstream 100 bases:

>100_bases
GCCGAGCACCGCGGAGACCGATTCGCGTGCCCGCGTGGTGGCCTCGCGCCGGCGACCCCGCTCGGGCGAATTCGTGGCCG
CGGGGCCCTGTAATACGGTG

Product: putative ferredoxin reductase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MSPREGTTAMSAPIVIVGAGLAGLRTAEELRRAGYEGDLVLLGDEARLPYDRPPLSKQFVRGETDDTTLRPAEFFTDKRI
ELRLGTTATGVDTATRRVLLADGSALAYDHLVIATGLRPRTLPGLPTPAGVHVLRDHADATALRDESASATAALVIGAGF
IGCEVAASFRARGLDVVLVEPQPTPLASVLGEQVGGLVARMHRAEGVDLRCGTGVRTLLSDDRGRVRGALLSDGAEVRAD
LVVLGVGSRPAVEWLADSGIALAEQAAGGGVLADEVGRTSVERVWAVGDVAAWRHETGAQQRVEHWTNAGEQAKLVACAL
LGAEPPTAARVPYFWSDQYDVKIQALGTPSADDDVSVAADDGRKFLAYYSRAGALTAVVGAGMTAQVMKARAKVAAGAPV
ADLLATT

Sequences:

>Translated_407_residues
MSPREGTTAMSAPIVIVGAGLAGLRTAEELRRAGYEGDLVLLGDEARLPYDRPPLSKQFVRGETDDTTLRPAEFFTDKRI
ELRLGTTATGVDTATRRVLLADGSALAYDHLVIATGLRPRTLPGLPTPAGVHVLRDHADATALRDESASATAALVIGAGF
IGCEVAASFRARGLDVVLVEPQPTPLASVLGEQVGGLVARMHRAEGVDLRCGTGVRTLLSDDRGRVRGALLSDGAEVRAD
LVVLGVGSRPAVEWLADSGIALAEQAAGGGVLADEVGRTSVERVWAVGDVAAWRHETGAQQRVEHWTNAGEQAKLVACAL
LGAEPPTAARVPYFWSDQYDVKIQALGTPSADDDVSVAADDGRKFLAYYSRAGALTAVVGAGMTAQVMKARAKVAAGAPV
ADLLATT
>Mature_406_residues
SPREGTTAMSAPIVIVGAGLAGLRTAEELRRAGYEGDLVLLGDEARLPYDRPPLSKQFVRGETDDTTLRPAEFFTDKRIE
LRLGTTATGVDTATRRVLLADGSALAYDHLVIATGLRPRTLPGLPTPAGVHVLRDHADATALRDESASATAALVIGAGFI
GCEVAASFRARGLDVVLVEPQPTPLASVLGEQVGGLVARMHRAEGVDLRCGTGVRTLLSDDRGRVRGALLSDGAEVRADL
VVLGVGSRPAVEWLADSGIALAEQAAGGGVLADEVGRTSVERVWAVGDVAAWRHETGAQQRVEHWTNAGEQAKLVACALL
GAEPPTAARVPYFWSDQYDVKIQALGTPSADDDVSVAADDGRKFLAYYSRAGALTAVVGAGMTAQVMKARAKVAAGAPVA
DLLATT

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=403, Percent_Identity=32.7543424317618, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI65787454, Length=403, Percent_Identity=32.7543424317618, Blast_Score=181, Evalue=2e-45,
Organism=Homo sapiens, GI226437568, Length=403, Percent_Identity=32.7543424317618, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI22202629, Length=347, Percent_Identity=26.2247838616715, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI4757732, Length=347, Percent_Identity=26.2247838616715, Blast_Score=99, Evalue=6e-21,
Organism=Escherichia coli, GI1788892, Length=386, Percent_Identity=30.8290155440415, Blast_Score=141, Evalue=7e-35,
Organism=Escherichia coli, GI1789065, Length=284, Percent_Identity=27.8169014084507, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1789765, Length=291, Percent_Identity=25.7731958762887, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17559934, Length=381, Percent_Identity=27.0341207349081, Blast_Score=137, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI32564386, Length=335, Percent_Identity=27.7611940298507, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24585130, Length=396, Percent_Identity=28.5353535353535, Blast_Score=154, Evalue=8e-38,
Organism=Drosophila melanogaster, GI281359715, Length=375, Percent_Identity=28, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI281359713, Length=375, Percent_Identity=28, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24639250, Length=375, Percent_Identity=28, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI18543267, Length=375, Percent_Identity=28, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24639252, Length=375, Percent_Identity=28, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24639257, Length=375, Percent_Identity=28, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI28573993, Length=362, Percent_Identity=25.6906077348066, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24581020, Length=362, Percent_Identity=25.6906077348066, Blast_Score=102, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 42425; Mature: 42294

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPREGTTAMSAPIVIVGAGLAGLRTAEELRRAGYEGDLVLLGDEARLPYDRPPLSKQFV
CCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCHHHH
RGETDDTTLRPAEFFTDKRIELRLGTTATGVDTATRRVLLADGSALAYDHLVIATGLRPR
CCCCCCCCCCCHHHHCCCEEEEEECCCCCCCCHHHCEEEEECCCEEEECEEEEEECCCCC
TLPGLPTPAGVHVLRDHADATALRDESASATAALVIGAGFIGCEVAASFRARGLDVVLVE
CCCCCCCCCCHHHHHCCCCHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHCCCEEEEEC
PQPTPLASVLGEQVGGLVARMHRAEGVDLRCGTGVRTLLSDDRGRVRGALLSDGAEVRAD
CCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHCCCCCCEEEHHHCCCCCEEEE
LVVLGVGSRPAVEWLADSGIALAEQAAGGGVLADEVGRTSVERVWAVGDVAAWRHETGAQ
EEEEECCCCCHHHHHHCCCCEEEHHCCCCCEEHHHHCCHHHHHHHHHHHHHHHHHHCCHH
QRVEHWTNAGEQAKLVACALLGAEPPTAARVPYFWSDQYDVKIQALGTPSADDDVSVAAD
HHHHHHHCCCCCHHEEEEEECCCCCCCCCCCCEEECCCEEEEEEEECCCCCCCCCEEECC
DGRKFLAYYSRAGALTAVVGAGMTAQVMKARAKVAAGAPVADLLATT
CCHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHCC
>Mature Secondary Structure 
SPREGTTAMSAPIVIVGAGLAGLRTAEELRRAGYEGDLVLLGDEARLPYDRPPLSKQFV
CCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCHHHH
RGETDDTTLRPAEFFTDKRIELRLGTTATGVDTATRRVLLADGSALAYDHLVIATGLRPR
CCCCCCCCCCCHHHHCCCEEEEEECCCCCCCCHHHCEEEEECCCEEEECEEEEEECCCCC
TLPGLPTPAGVHVLRDHADATALRDESASATAALVIGAGFIGCEVAASFRARGLDVVLVE
CCCCCCCCCCHHHHHCCCCHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHCCCEEEEEC
PQPTPLASVLGEQVGGLVARMHRAEGVDLRCGTGVRTLLSDDRGRVRGALLSDGAEVRAD
CCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHCCCCCCEEEHHHCCCCCEEEE
LVVLGVGSRPAVEWLADSGIALAEQAAGGGVLADEVGRTSVERVWAVGDVAAWRHETGAQ
EEEEECCCCCHHHHHHCCCCEEEHHCCCCCEEHHHHCCHHHHHHHHHHHHHHHHHHCCHH
QRVEHWTNAGEQAKLVACALLGAEPPTAARVPYFWSDQYDVKIQALGTPSADDDVSVAAD
HHHHHHHCCCCCHHEEEEEECCCCCCCCCCCCEEECCCEEEEEEEECCCCCCCCCEEECC
DGRKFLAYYSRAGALTAVVGAGMTAQVMKARAKVAAGAPVADLLATT
CCHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]