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The map label for this gene is murI [H]

Identifier: 54027044

GI number: 54027044

Start: 5369457

End: 5370218

Strand: Reverse

Name: murI [H]

Synonym: nfa50700

Alternate gene names: 54027044

Gene position: 5370218-5369457 (Counterclockwise)

Preceding gene: 54027045

Following gene: 54027043

Centisome position: 89.19

GC content: 74.67

Gene sequence:

>762_bases
GTGATCGTCGCGCTCATCGACTCGGGTCTGGGGCTGCTGCCAACGGCCGCGTGGCTGCGCAAGCTGCGCCCGGATGTGGA
TCTGCTGCTGCAACTGGACCCCGACGGCGCGCCCTGGGGGCCCAAGCCGGAGCAGTGGACGATCGAGCGGGTGGTGGCGG
CGGCGCGTATCTCCATCGCCGCCGGGGCCGAGGTGATCGTGCTGCCCTGCAACACCGCCAGCGTGACCGCGCTCGAGCAG
GTACGCGCCGAGGTCGGGCCCGAGGTGCCGGTGATCGGCACGGTGCCCGCGATCAAGCCCGCCGCCGCGGCCTGCCGCCG
GGTGGCGGTGTGGGCGACGGCCGCGACCACGGCCAGCCGCTATCAGGCGGAGCTGATCGAGAAGTTCGGTGGTGACGCCG
AGGTGGTCGGGGTGGCCTGCCACGGCCTCGCCGACGCCATCGACCGCGGTGACCTCACCGCGGCCACCGACGCGATCGCC
CGCGCGGCCGCGCAGACCCCGGCGGGCACCGAGGGCGTGGTTCTGGGCTGCACGCACTATCCGCTGGTCGTCGACGCCAT
CGTCGCCGCGCTGCCCGCGGGGGTGCGGCTGTTCGACAGTGCGCAGGCCGTGGCGGCGCAGACCATCCGCAAGATGGAGG
GGCTCGGCAGGCCGACCACCGGGGAGGGCGTGGTCCGCGTCCTCAACAGCGGCAGGCCGGGCACGCTGCCCCCCAGCGCC
GCCGCCTATGAACCGGGACGCATCCTCGGCGCCCAGCCCTGA

Upstream 100 bases:

>100_bases
CACCGCGGAGACCGATTCGCGTGCCCGCGTGGTGGCCTCGCGCCGGCGACCCCGCTCGGGCGAATTCGTGGCCGCGGGGC
CCTGTAATACGGTGGTCGAT

Downstream 100 bases:

>100_bases
CACCGCGGTCGGCGGCCGCCCGCCGATTAGCCTTGCGCGAGATGTGTCGAGGAGGTCCGCGATGAGTCCATCGGTGTCCA
CCGCCGAGCAGGTCCGCGCC

Product: putative glutamate racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MIVALIDSGLGLLPTAAWLRKLRPDVDLLLQLDPDGAPWGPKPEQWTIERVVAAARISIAAGAEVIVLPCNTASVTALEQ
VRAEVGPEVPVIGTVPAIKPAAAACRRVAVWATAATTASRYQAELIEKFGGDAEVVGVACHGLADAIDRGDLTAATDAIA
RAAAQTPAGTEGVVLGCTHYPLVVDAIVAALPAGVRLFDSAQAVAAQTIRKMEGLGRPTTGEGVVRVLNSGRPGTLPPSA
AAYEPGRILGAQP

Sequences:

>Translated_253_residues
MIVALIDSGLGLLPTAAWLRKLRPDVDLLLQLDPDGAPWGPKPEQWTIERVVAAARISIAAGAEVIVLPCNTASVTALEQ
VRAEVGPEVPVIGTVPAIKPAAAACRRVAVWATAATTASRYQAELIEKFGGDAEVVGVACHGLADAIDRGDLTAATDAIA
RAAAQTPAGTEGVVLGCTHYPLVVDAIVAALPAGVRLFDSAQAVAAQTIRKMEGLGRPTTGEGVVRVLNSGRPGTLPPSA
AAYEPGRILGAQP
>Mature_253_residues
MIVALIDSGLGLLPTAAWLRKLRPDVDLLLQLDPDGAPWGPKPEQWTIERVVAAARISIAAGAEVIVLPCNTASVTALEQ
VRAEVGPEVPVIGTVPAIKPAAAACRRVAVWATAATTASRYQAELIEKFGGDAEVVGVACHGLADAIDRGDLTAATDAIA
RAAAQTPAGTEGVVLGCTHYPLVVDAIVAALPAGVRLFDSAQAVAAQTIRKMEGLGRPTTGEGVVRVLNSGRPGTLPPSA
AAYEPGRILGAQP

Specific function: Provides the (R)-glutamate required for cell wall biosynthesis [H]

COG id: COG0796

COG function: function code M; Glutamate racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartate/glutamate racemases family [H]

Homologues:

Organism=Escherichia coli, GI87082355, Length=224, Percent_Identity=36.1607142857143, Blast_Score=98, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015942
- InterPro:   IPR001920
- InterPro:   IPR018187
- InterPro:   IPR004391 [H]

Pfam domain/function: PF01177 Asp_Glu_race [H]

EC number: =5.1.1.3 [H]

Molecular weight: Translated: 25864; Mature: 25864

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00923 ASP_GLU_RACEMASE_1 ; PS00924 ASP_GLU_RACEMASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVALIDSGLGLLPTAAWLRKLRPDVDLLLQLDPDGAPWGPKPEQWTIERVVAAARISIA
CEEEEECCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHEE
AGAEVIVLPCNTASVTALEQVRAEVGPEVPVIGTVPAIKPAAAACRRVAVWATAATTASR
CCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
YQAELIEKFGGDAEVVGVACHGLADAIDRGDLTAATDAIARAAAQTPAGTEGVVLGCTHY
HHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCH
PLVVDAIVAALPAGVRLFDSAQAVAAQTIRKMEGLGRPTTGEGVVRVLNSGRPGTLPPSA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCC
AAYEPGRILGAQP
CCCCCCCCCCCCC
>Mature Secondary Structure
MIVALIDSGLGLLPTAAWLRKLRPDVDLLLQLDPDGAPWGPKPEQWTIERVVAAARISIA
CEEEEECCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHEE
AGAEVIVLPCNTASVTALEQVRAEVGPEVPVIGTVPAIKPAAAACRRVAVWATAATTASR
CCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
YQAELIEKFGGDAEVVGVACHGLADAIDRGDLTAATDAIARAAAQTPAGTEGVVLGCTHY
HHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCH
PLVVDAIVAALPAGVRLFDSAQAVAAQTIRKMEGLGRPTTGEGVVRVLNSGRPGTLPPSA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCC
AAYEPGRILGAQP
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA