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The map label for this gene is cmek

Identifier: 54026875

GI number: 54026875

Start: 5115992

End: 5116915

Strand: Reverse

Name: cmek

Synonym: nfa49010

Alternate gene names: 54026875

Gene position: 5116915-5115992 (Counterclockwise)

Preceding gene: 54026877

Following gene: 54026874

Centisome position: 84.98

GC content: 74.68

Gene sequence:

>924_bases
GTGCTGTCAGTTGTGCCCAGTCCGGTTCTCGTGCGCGCGCCCTCGAAGGTGAACCTGCACCTCGGTGTCGGCGACCTGCG
CCCCGACGGCTACCACGACCTGACCACGGTGTTCCAGGCGCTGTCCCTCGGCGACGACCTGCGGATCTCGCCCGCCTCGG
CGCTCACGGTGAAGGTGAACGGCGAGGGCGCCGCCGAGGTGCCCACCGATCGCACGAATCTGGTGTGGAAGGCCGCGGTG
CGCTTGGCCCACCTGGTCGGGCGGGCGCCACTGGTGGAGATCGTCATCGACAAGGGCATCCCGGTGGCGGGCGGGATGGC
CGGCGGCAGCGCCGACGCCGCCGCCGCGCTGGTCGGCCTCAACGAGCTGTGGGACATGGGCCTGGGCCGTGACGAATTGT
CCGCGCTCGCCGCCGAACTCGGCAGCGACGTGCCCTTTGCCCTGCACGGCGGCACCGCGCTGGGCACCGGCCGGGGCGAG
CGATTGCTTCCGGTGCTGTCGCGCAACACCTTTCACTGGGTGCTCGCCTTCGCCAAGGGCGGGCTGTCCACCCCGGCGGT
GTTCGCCGAACTCGACCGGCTGCGCGAGCACGGCGACCCGCCGCGCCTCGGCGACCCGCAGGCCCTGATGCAGGCGCTGG
CCTCCGGCGACCCCGCCCAGCTCGCGCCGCTGCTCGGCAACGACCTACAGGCCGCGGCGGTCTCGTTGAAGCCCGAGCTG
CGCCGCACGCTGCGCGCGGGCGTCACCGCGGGCGCGCTGGCCGGGCTGGTCTCCGGCTCCGGACCCACCTGTGCGTTCCT
GTGCGAGAGCGCGGACGCCGCCGTCGCGGTCGCGGCGGAGCTGGCCGGGGCGGGCGTGGCCCGCAGCGTGCGCACCGCGA
GCGGGCCGGTGCCCGGCGCGCGCGTCATCGATCCGGAGAGCTGA

Upstream 100 bases:

>100_bases
GTACGCAAGGCGGTGCGCGCATCGGGACGCGGCGGACACCGCCGTCCCGGCCGCTCGTGAGCCCGGCGGGTGTCGGGGCC
AGCGGATAGGCTTGGGCCTC

Downstream 100 bases:

>100_bases
CCAGGTCCGACGAACCGGCCCCCGACCCTGCCTCGGTACCCTGGCTCGGGGCCCTGCCGGGCCCGCGGCACCGGTATGTC
GGAAGGATCCATGTCCAACC

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MLSVVPSPVLVRAPSKVNLHLGVGDLRPDGYHDLTTVFQALSLGDDLRISPASALTVKVNGEGAAEVPTDRTNLVWKAAV
RLAHLVGRAPLVEIVIDKGIPVAGGMAGGSADAAAALVGLNELWDMGLGRDELSALAAELGSDVPFALHGGTALGTGRGE
RLLPVLSRNTFHWVLAFAKGGLSTPAVFAELDRLREHGDPPRLGDPQALMQALASGDPAQLAPLLGNDLQAAAVSLKPEL
RRTLRAGVTAGALAGLVSGSGPTCAFLCESADAAVAVAAELAGAGVARSVRTASGPVPGARVIDPES

Sequences:

>Translated_307_residues
MLSVVPSPVLVRAPSKVNLHLGVGDLRPDGYHDLTTVFQALSLGDDLRISPASALTVKVNGEGAAEVPTDRTNLVWKAAV
RLAHLVGRAPLVEIVIDKGIPVAGGMAGGSADAAAALVGLNELWDMGLGRDELSALAAELGSDVPFALHGGTALGTGRGE
RLLPVLSRNTFHWVLAFAKGGLSTPAVFAELDRLREHGDPPRLGDPQALMQALASGDPAQLAPLLGNDLQAAAVSLKPEL
RRTLRAGVTAGALAGLVSGSGPTCAFLCESADAAVAVAAELAGAGVARSVRTASGPVPGARVIDPES
>Mature_307_residues
MLSVVPSPVLVRAPSKVNLHLGVGDLRPDGYHDLTTVFQALSLGDDLRISPASALTVKVNGEGAAEVPTDRTNLVWKAAV
RLAHLVGRAPLVEIVIDKGIPVAGGMAGGSADAAAALVGLNELWDMGLGRDELSALAAELGSDVPFALHGGTALGTGRGE
RLLPVLSRNTFHWVLAFAKGGLSTPAVFAELDRLREHGDPPRLGDPQALMQALASGDPAQLAPLLGNDLQAAAVSLKPEL
RRTLRAGVTAGALAGLVSGSGPTCAFLCESADAAVAVAAELAGAGVARSVRTASGPVPGARVIDPES

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=188, Percent_Identity=36.1702127659575, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1786184, Length=279, Percent_Identity=26.8817204301075, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_NOCFA (Q5YPY8)

Other databases:

- EMBL:   AP006618
- RefSeq:   YP_121117.1
- ProteinModelPortal:   Q5YPY8
- SMR:   Q5YPY8
- GeneID:   3109844
- GenomeReviews:   AP006618_GR
- KEGG:   nfa:nfa49010
- NMPDR:   fig|247156.1.peg.4714
- HOGENOM:   HBG734593
- OMA:   RIYNGLR
- ProtClustDB:   PRK03188
- BioCyc:   NFAR247156:NFA49010-MONOMER
- BRENDA:   2.7.1.148
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 31124; Mature: 31124

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: ACT_SITE 16-16 ACT_SITE 143-143

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSVVPSPVLVRAPSKVNLHLGVGDLRPDGYHDLTTVFQALSLGDDLRISPASALTVKVN
CCCCCCCCEEEECCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCEECCCCEEEEEEC
GEGAAEVPTDRTNLVWKAAVRLAHLVGRAPLVEIVIDKGIPVAGGMAGGSADAAAALVGL
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHH
NELWDMGLGRDELSALAAELGSDVPFALHGGTALGTGRGERLLPVLSRNTFHWVLAFAKG
HHHHHCCCCHHHHHHHHHHHCCCCCEEEECCCEECCCCCCEEHHHHHCCCCEEEEEECCC
GLSTPAVFAELDRLREHGDPPRLGDPQALMQALASGDPAQLAPLLGNDLQAAAVSLKPEL
CCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHCCHHH
RRTLRAGVTAGALAGLVSGSGPTCAFLCESADAAVAVAAELAGAGVARSVRTASGPVPGA
HHHHHHCCHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCHHHHHHHHCCCCCCCC
RVIDPES
EECCCCC
>Mature Secondary Structure
MLSVVPSPVLVRAPSKVNLHLGVGDLRPDGYHDLTTVFQALSLGDDLRISPASALTVKVN
CCCCCCCCEEEECCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCEECCCCEEEEEEC
GEGAAEVPTDRTNLVWKAAVRLAHLVGRAPLVEIVIDKGIPVAGGMAGGSADAAAALVGL
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHH
NELWDMGLGRDELSALAAELGSDVPFALHGGTALGTGRGERLLPVLSRNTFHWVLAFAKG
HHHHHCCCCHHHHHHHHHHHCCCCCEEEECCCEECCCCCCEEHHHHHCCCCEEEEEECCC
GLSTPAVFAELDRLREHGDPPRLGDPQALMQALASGDPAQLAPLLGNDLQAAAVSLKPEL
CCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHCCHHH
RRTLRAGVTAGALAGLVSGSGPTCAFLCESADAAVAVAAELAGAGVARSVRTASGPVPGA
HHHHHHCCHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCHHHHHHHHCCCCCCCC
RVIDPES
EECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA