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The map label for this gene is ksgA

Identifier: 54026877

GI number: 54026877

Start: 5117444

End: 5118325

Strand: Reverse

Name: ksgA

Synonym: nfa49030

Alternate gene names: 54026877

Gene position: 5118325-5117444 (Counterclockwise)

Preceding gene: 54026878

Following gene: 54026875

Centisome position: 85.0

GC content: 74.49

Gene sequence:

>882_bases
GTGTCCGAAGCCGATGTCACCGCCCGCGGCGATGCCGCGTTGCTCGGGCCCGCCGAGGTGCGGGCGCTGGCCGAGCGGTT
CGGCGTGCGGCCGACCAAGCAGCTCGGGCAGAACTTCGTCCACGACGCGAACACGGTGCGTCGCATCGTCACCGCGGCGG
GCGTCGGCCGCGCCGACACGGTGCTGGAGGTCGGTCCCGGCCTCGGTTCGCTGACGCTGGCCCTGCTCGACGTGGTCGAT
TCGGTGGTGGCCGTCGAGATCGATCCGGTGCTCGCCGAGCATCTCCCGCGCACGGTGGCCGATCGCGCGCCCGCGTTGGC
GGGGCGGTTGCGGGTGGTGCGGGACGACGCGCTGCGGGTGCGGGCAGCCGACCTGCCCGCGAGCCCGACCGCGCTGGTGG
CCAATCTGCCCTACAACGTGGCCGTGCCGGTGCTGTTGCATCTGCTGGCCGAGTTGCCCGGCCTGCGTACGGCGCTGGTG
ATGGTGCAGGCCGAGGTGGCCGACCGGCTGGCTGCCGAGCCGGGCAGCCGGGTCTACGGTGTGCCCAGCGTGAAGGCCGG
ATTCTTCGGCACGGTGCGGCGGGCCGGTGCGGTGGGCACGCAGGTCTTCTGGCCGGTGCCGCGGGTGGAATCGGGACTGG
TGCGGGTGGAGCGCTACGTCGAGCCGCCGTGGCCGATGGACGAGCAGCACCGCCGCCGGGTCTTCGAGATCATCGATGCG
GCCTTCGCGCAGCGGCGCAAGACGCTGCGCGCGGCGCTGGCGGGCTGGGCGGGCAGTCCCGCCGAGGCCGAACGGCGGCT
GCTGGCCGCGGGGATCGATCCCACGGCCCGGGGCGAGACGCTCGACACCGCCGCCTATGTGCGGCTGGCGGCGCAGGCCT
GA

Upstream 100 bases:

>100_bases
TGACCACGGAGGCCGCAGCGGGGCGCCATCCGTCGGGGGTGGCGCCCCGTTCCGTGCCCGTGCCGGGCCGCGCGGTCCGG
CGGATTGGTAGGTTCGTCCG

Downstream 100 bases:

>100_bases
CCGCGCGTTGTCCGAGAGGGACAACGGTATTCGCCCGGGCCGTCGAGCGATCGGTGTCGCGGCGGCGGCGCCGGGTCCGC
CGAACGAACCCGGCGGCCGC

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MSEADVTARGDAALLGPAEVRALAERFGVRPTKQLGQNFVHDANTVRRIVTAAGVGRADTVLEVGPGLGSLTLALLDVVD
SVVAVEIDPVLAEHLPRTVADRAPALAGRLRVVRDDALRVRAADLPASPTALVANLPYNVAVPVLLHLLAELPGLRTALV
MVQAEVADRLAAEPGSRVYGVPSVKAGFFGTVRRAGAVGTQVFWPVPRVESGLVRVERYVEPPWPMDEQHRRRVFEIIDA
AFAQRRKTLRAALAGWAGSPAEAERRLLAAGIDPTARGETLDTAAYVRLAAQA

Sequences:

>Translated_293_residues
MSEADVTARGDAALLGPAEVRALAERFGVRPTKQLGQNFVHDANTVRRIVTAAGVGRADTVLEVGPGLGSLTLALLDVVD
SVVAVEIDPVLAEHLPRTVADRAPALAGRLRVVRDDALRVRAADLPASPTALVANLPYNVAVPVLLHLLAELPGLRTALV
MVQAEVADRLAAEPGSRVYGVPSVKAGFFGTVRRAGAVGTQVFWPVPRVESGLVRVERYVEPPWPMDEQHRRRVFEIIDA
AFAQRRKTLRAALAGWAGSPAEAERRLLAAGIDPTARGETLDTAAYVRLAAQA
>Mature_292_residues
SEADVTARGDAALLGPAEVRALAERFGVRPTKQLGQNFVHDANTVRRIVTAAGVGRADTVLEVGPGLGSLTLALLDVVDS
VVAVEIDPVLAEHLPRTVADRAPALAGRLRVVRDDALRVRAADLPASPTALVANLPYNVAVPVLLHLLAELPGLRTALVM
VQAEVADRLAAEPGSRVYGVPSVKAGFFGTVRRAGAVGTQVFWPVPRVESGLVRVERYVEPPWPMDEQHRRRVFEIIDAA
FAQRRKTLRAALAGWAGSPAEAERRLLAAGIDPTARGETLDTAAYVRLAAQA

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI7657198, Length=223, Percent_Identity=36.322869955157, Blast_Score=124, Evalue=8e-29,
Organism=Homo sapiens, GI156415992, Length=295, Percent_Identity=29.8305084745763, Blast_Score=95, Evalue=7e-20,
Organism=Escherichia coli, GI1786236, Length=261, Percent_Identity=31.8007662835249, Blast_Score=133, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI25146882, Length=218, Percent_Identity=33.0275229357798, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI25141369, Length=303, Percent_Identity=26.0726072607261, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324989, Length=191, Percent_Identity=33.5078534031414, Blast_Score=98, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21358017, Length=229, Percent_Identity=33.1877729257642, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI21357273, Length=288, Percent_Identity=27.7777777777778, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_NOCFA (Q5YPY6)

Other databases:

- EMBL:   AP006618
- RefSeq:   YP_121119.1
- ProteinModelPortal:   Q5YPY6
- SMR:   Q5YPY6
- GeneID:   3111203
- GenomeReviews:   AP006618_GR
- KEGG:   nfa:nfa49030
- NMPDR:   fig|247156.1.peg.4716
- HOGENOM:   HBG319664
- OMA:   TPIIMKL
- ProtClustDB:   PRK00274
- BioCyc:   NFAR247156:NFA49030-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 31176; Mature: 31045

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 38-38 BINDING 40-40 BINDING 65-65 BINDING 86-86 BINDING 116-116 BINDING 135-135

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEADVTARGDAALLGPAEVRALAERFGVRPTKQLGQNFVHDANTVRRIVTAAGVGRADT
CCCCCCCCCCCEEEECHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
VLEVGPGLGSLTLALLDVVDSVVAVEIDPVLAEHLPRTVADRAPALAGRLRVVRDDALRV
HEEECCCHHHHHHHHHHHHHHHHHEEECHHHHHHCCHHHHHHCHHHHHHHHHEEHHHHEE
RAADLPASPTALVANLPYNVAVPVLLHLLAELPGLRTALVMVQAEVADRLAAEPGSRVYG
EECCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEC
VPSVKAGFFGTVRRAGAVGTQVFWPVPRVESGLVRVERYVEPPWPMDEQHRRRVFEIIDA
CCCCCHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
AFAQRRKTLRAALAGWAGSPAEAERRLLAAGIDPTARGETLDTAAYVRLAAQA
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
SEADVTARGDAALLGPAEVRALAERFGVRPTKQLGQNFVHDANTVRRIVTAAGVGRADT
CCCCCCCCCCEEEECHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
VLEVGPGLGSLTLALLDVVDSVVAVEIDPVLAEHLPRTVADRAPALAGRLRVVRDDALRV
HEEECCCHHHHHHHHHHHHHHHHHEEECHHHHHHCCHHHHHHCHHHHHHHHHEEHHHHEE
RAADLPASPTALVANLPYNVAVPVLLHLLAELPGLRTALVMVQAEVADRLAAEPGSRVYG
EECCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEC
VPSVKAGFFGTVRRAGAVGTQVFWPVPRVESGLVRVERYVEPPWPMDEQHRRRVFEIIDA
CCCCCHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
AFAQRRKTLRAALAGWAGSPAEAERRLLAAGIDPTARGETLDTAAYVRLAAQA
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA