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The map label for this gene is glyA

Identifier: 54026787

GI number: 54026787

Start: 5027484

End: 5028779

Strand: Reverse

Name: glyA

Synonym: nfa48130

Alternate gene names: 54026787

Gene position: 5028779-5027484 (Counterclockwise)

Preceding gene: 54026788

Following gene: 54026783

Centisome position: 83.52

GC content: 69.98

Gene sequence:

>1296_bases
GTGACGCAGACGACCGCCTCTGTCAATACCCAGTCTCTCGGTGAGCTCGATCCCGAGCTCGCGGCCGCGATGGCAGGCGA
ACTCGCCCGCGAGCGCGACACTCTCGAGATGATCGCCTCGGAGAACTTCGTGCCGCGCGCGGTGCTGCAGGCCCAGGGCA
GCGTGCTGACCAACAAGTACGCCGAGGGTTACCCGGGGCGTCGCTACTACGGCGGTTGCGAGAACGTCGACGTCGTCGAG
AACCTGGCCCGCGAGCGCGCCAAGGAACTGTTCGGCGCCGAATTCGCCAACGTGCAGCCGCATTCCGGCGCGCAGGCCAA
CGCGGCGGTGCTGATGTCGCTGATGGATCCCGGCGACCGGCTGCTCGGCCTCGACCTCGCGCACGGCGGCCATCTCACCC
ACGGCATGCGGCTGAACTTCTCCGGCAAGCTCTACGAGGTGCACTCCTACGGGGTGAGCAAGGAAGACCACCGCGTCGAC
ATGGACGAGGTGCGCACGATCGCGCTCGGCGCCAAGCCGAAGGTGATCGTCGCGGGCTGGTCGGCCTACCCGCGTCACCA
GGACTTCGCCGCCTTCCGCGCGATCGCCGACGAGGTGGGTGCCTACCTGTGGGTGGACATGGCGCACTTCGCCGGTCTCG
TCGCCGCGGGCCTGCACCCCTCGCCGGTGCCCTACGCCGATGTCGTCTCCTCCACCGTGCACAAGACCCTCGGCGGTCCC
CGCTCCGGCCTGATCCTGGCCAAGCAGGAGTTCGCCAAGAAGATCAACAGCGCGGTCTTCCCCGGCCAGCAGGGCGGTCC
GCTCATGCACGCGATCGCGGCCAAGGCGGTGGCCTTCAAGATCGCGGGCACCGAGGAGTTCCGCGACCGCCAGCAGCGCA
CCCTGTCGGGCGCGAAGATCCTGGCCGAGCGGCTCACCGGCGCCGACGTCGCCGACAAGGGCATCAGCGTGCTCACCGGC
GGCACCGACGTGCACCTGGTGCTGGTCGATCTGCGCAACTCCCAGCTCGACGGTCAGCAGGGCGAGGACCTGCTGCACGA
GATCGGCATCACCGTCAACCGCAACGCCGTGCCGTTCGACCCCCGGCCGCCGATGGTCACCTCCGGTCTGCGGATCGGCA
CCGCCGCCCTGGCCACCCGCGGCTTCGGCGACGCCGAGTTCACCGAGGTCGCCGACATCATCGCCACCGCGCTGGCCGGC
GGCTCCGATGCGGAGACGCTGCGCGGTCGGGTGCGCGCGCTGGCTCAGCGCGTGCCGCTCTACCAGGGCCTGGAGGATTG
GCACCTGCTCGGCTGA

Upstream 100 bases:

>100_bases
GTGGCGGAGAATCTGCGCGTGCTGCTGGCCGCGGAGACGGCGGGGCCGGGCCGGTAACCCCGTGCGCCGCACGCCACCGG
CGCCCCCATAGACTGAGGCC

Downstream 100 bases:

>100_bases
GCCACGCACCGACGACCGTCGTCCGAGGCGCGGCGGTGCCGCCGCCCCGCTCGGGCAGCTCGACAACGCGAACGGCCCGG
TCCGCACCCCTCGTCCGAGG

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MTQTTASVNTQSLGELDPELAAAMAGELARERDTLEMIASENFVPRAVLQAQGSVLTNKYAEGYPGRRYYGGCENVDVVE
NLARERAKELFGAEFANVQPHSGAQANAAVLMSLMDPGDRLLGLDLAHGGHLTHGMRLNFSGKLYEVHSYGVSKEDHRVD
MDEVRTIALGAKPKVIVAGWSAYPRHQDFAAFRAIADEVGAYLWVDMAHFAGLVAAGLHPSPVPYADVVSSTVHKTLGGP
RSGLILAKQEFAKKINSAVFPGQQGGPLMHAIAAKAVAFKIAGTEEFRDRQQRTLSGAKILAERLTGADVADKGISVLTG
GTDVHLVLVDLRNSQLDGQQGEDLLHEIGITVNRNAVPFDPRPPMVTSGLRIGTAALATRGFGDAEFTEVADIIATALAG
GSDAETLRGRVRALAQRVPLYQGLEDWHLLG

Sequences:

>Translated_431_residues
MTQTTASVNTQSLGELDPELAAAMAGELARERDTLEMIASENFVPRAVLQAQGSVLTNKYAEGYPGRRYYGGCENVDVVE
NLARERAKELFGAEFANVQPHSGAQANAAVLMSLMDPGDRLLGLDLAHGGHLTHGMRLNFSGKLYEVHSYGVSKEDHRVD
MDEVRTIALGAKPKVIVAGWSAYPRHQDFAAFRAIADEVGAYLWVDMAHFAGLVAAGLHPSPVPYADVVSSTVHKTLGGP
RSGLILAKQEFAKKINSAVFPGQQGGPLMHAIAAKAVAFKIAGTEEFRDRQQRTLSGAKILAERLTGADVADKGISVLTG
GTDVHLVLVDLRNSQLDGQQGEDLLHEIGITVNRNAVPFDPRPPMVTSGLRIGTAALATRGFGDAEFTEVADIIATALAG
GSDAETLRGRVRALAQRVPLYQGLEDWHLLG
>Mature_430_residues
TQTTASVNTQSLGELDPELAAAMAGELARERDTLEMIASENFVPRAVLQAQGSVLTNKYAEGYPGRRYYGGCENVDVVEN
LARERAKELFGAEFANVQPHSGAQANAAVLMSLMDPGDRLLGLDLAHGGHLTHGMRLNFSGKLYEVHSYGVSKEDHRVDM
DEVRTIALGAKPKVIVAGWSAYPRHQDFAAFRAIADEVGAYLWVDMAHFAGLVAAGLHPSPVPYADVVSSTVHKTLGGPR
SGLILAKQEFAKKINSAVFPGQQGGPLMHAIAAKAVAFKIAGTEEFRDRQQRTLSGAKILAERLTGADVADKGISVLTGG
TDVHLVLVDLRNSQLDGQQGEDLLHEIGITVNRNAVPFDPRPPMVTSGLRIGTAALATRGFGDAEFTEVADIIATALAGG
SDAETLRGRVRALAQRVPLYQGLEDWHLLG

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI261862352, Length=408, Percent_Identity=44.8529411764706, Blast_Score=323, Evalue=2e-88,
Organism=Homo sapiens, GI261862350, Length=408, Percent_Identity=44.8529411764706, Blast_Score=323, Evalue=2e-88,
Organism=Homo sapiens, GI261862348, Length=408, Percent_Identity=44.8529411764706, Blast_Score=323, Evalue=2e-88,
Organism=Homo sapiens, GI19923315, Length=408, Percent_Identity=44.8529411764706, Blast_Score=322, Evalue=3e-88,
Organism=Homo sapiens, GI261862346, Length=411, Percent_Identity=44.7688564476886, Blast_Score=314, Evalue=1e-85,
Organism=Homo sapiens, GI22547186, Length=408, Percent_Identity=43.6274509803922, Blast_Score=299, Evalue=4e-81,
Organism=Homo sapiens, GI22547189, Length=393, Percent_Identity=41.2213740458015, Blast_Score=266, Evalue=4e-71,
Organism=Escherichia coli, GI1788902, Length=416, Percent_Identity=53.6057692307692, Blast_Score=419, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI25144732, Length=406, Percent_Identity=44.0886699507389, Blast_Score=316, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI25144729, Length=406, Percent_Identity=44.0886699507389, Blast_Score=316, Evalue=2e-86,
Organism=Saccharomyces cerevisiae, GI6319739, Length=431, Percent_Identity=42.4593967517401, Blast_Score=335, Evalue=6e-93,
Organism=Saccharomyces cerevisiae, GI6323087, Length=413, Percent_Identity=40.4358353510896, Blast_Score=304, Evalue=2e-83,
Organism=Drosophila melanogaster, GI24640005, Length=420, Percent_Identity=45.9523809523809, Blast_Score=351, Evalue=7e-97,
Organism=Drosophila melanogaster, GI221329721, Length=420, Percent_Identity=45.9523809523809, Blast_Score=350, Evalue=1e-96,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_NOCFA (Q5YQ76)

Other databases:

- EMBL:   AP006618
- RefSeq:   YP_121029.1
- ProteinModelPortal:   Q5YQ76
- GeneID:   3109606
- GenomeReviews:   AP006618_GR
- KEGG:   nfa:nfa48130
- NMPDR:   fig|247156.1.peg.4627
- HOGENOM:   HBG301263
- OMA:   MILTNHE
- ProtClustDB:   PRK00011
- BioCyc:   NFAR247156:NFA48130-MONOMER
- BRENDA:   2.1.2.1
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 46061; Mature: 45930

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQTTASVNTQSLGELDPELAAAMAGELARERDTLEMIASENFVPRAVLQAQGSVLTNKY
CCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHH
AEGYPGRRYYGGCENVDVVENLARERAKELFGAEFANVQPHSGAQANAAVLMSLMDPGDR
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCHHHHHHHHHHCCCCC
LLGLDLAHGGHLTHGMRLNFSGKLYEVHSYGVSKEDHRVDMDEVRTIALGAKPKVIVAGW
EEEEEECCCCCCCCCEEECCCCCEEEEECCCCCCCCCCCCHHHHHEEEECCCCCEEEECC
SAYPRHQDFAAFRAIADEVGAYLWVDMAHFAGLVAAGLHPSPVPYADVVSSTVHKTLGGP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCC
RSGLILAKQEFAKKINSAVFPGQQGGPLMHAIAAKAVAFKIAGTEEFRDRQQRTLSGAKI
CCCEEEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHH
LAERLTGADVADKGISVLTGGTDVHLVLVDLRNSQLDGQQGEDLLHEIGITVNRNAVPFD
HHHHHCCCCHHCCCCEEEECCCEEEEEEEEECCCCCCCCCHHHHHHHCCCEECCCCCCCC
PRPPMVTSGLRIGTAALATRGFGDAEFTEVADIIATALAGGSDAETLRGRVRALAQRVPL
CCCCCEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCHH
YQGLEDWHLLG
HCCCCHHHCCC
>Mature Secondary Structure 
TQTTASVNTQSLGELDPELAAAMAGELARERDTLEMIASENFVPRAVLQAQGSVLTNKY
CCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHH
AEGYPGRRYYGGCENVDVVENLARERAKELFGAEFANVQPHSGAQANAAVLMSLMDPGDR
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCHHHHHHHHHHCCCCC
LLGLDLAHGGHLTHGMRLNFSGKLYEVHSYGVSKEDHRVDMDEVRTIALGAKPKVIVAGW
EEEEEECCCCCCCCCEEECCCCCEEEEECCCCCCCCCCCCHHHHHEEEECCCCCEEEECC
SAYPRHQDFAAFRAIADEVGAYLWVDMAHFAGLVAAGLHPSPVPYADVVSSTVHKTLGGP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCC
RSGLILAKQEFAKKINSAVFPGQQGGPLMHAIAAKAVAFKIAGTEEFRDRQQRTLSGAKI
CCCEEEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHH
LAERLTGADVADKGISVLTGGTDVHLVLVDLRNSQLDGQQGEDLLHEIGITVNRNAVPFD
HHHHHCCCCHHCCCCEEEECCCEEEEEEEEECCCCCCCCCHHHHHHHCCCEECCCCCCCC
PRPPMVTSGLRIGTAALATRGFGDAEFTEVADIIATALAGGSDAETLRGRVRALAQRVPL
CCCCCEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCHH
YQGLEDWHLLG
HCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA