Click here to switch to the map view.

The map label for this gene is folP2 [H]

Identifier: 54026711

GI number: 54026711

Start: 4947722

End: 4948663

Strand: Reverse

Name: folP2 [H]

Synonym: nfa47370

Alternate gene names: 54026711

Gene position: 4948663-4947722 (Counterclockwise)

Preceding gene: 54026712

Following gene: 54026710

Centisome position: 82.19

GC content: 72.19

Gene sequence:

>942_bases
GTGGTCATGTTCAGCCCGCCCGCAGCGCCGCTGCCCACCCTGTGCGGCAAGCCGGTCGCGACGGACCGGGCGCTGGTGAT
GGCCATCGTCAACCGCACCCCGGACTCGTTCTACGACCGCGGCGCCACCTTCACCGACGAGGCCGCGATGGCCGCCGTCG
ACCGCGCGGTCGCCGAGGGCGCCGACCTGGTGGACATCGGCGGGGTGAAGGCGGGCCCGGGCAGCGAGGTCGACGCCGCC
GTGGACATCGGCGGGGTGAAGGCGGGCCCGGGCAGCGAGGTCGACGCCGCCGAGGAGACGCGGCGGGTGGTGCCGTTCGT
GGCCGCCATCCGCGCCGCCTATCCCGACCTGCTGATCAGCGTCGACACCTGGCGTTCGGAGGTCGCGCGGGCCGCCGTGG
CCGAGGGCGCCGACCTGATCAACGACACCTGGGCCGGCGCCGACCCGGAACTGGTGCGGGTGGCCGCCGAACACGGTGCG
GGCATCGTGTGCAGCCACACCGGCGGCGCCGTTCCGCGCACCCGCCCGCACCGCGTCCGCTACGCCGACGTGGTGGCCGA
GGTCACCGAGACCGTCGTGGCCGCCGCCGAGCGGGCGGCCGCGGCGGGCGTGCGCACGGACTCGATCCTCATCGATCCGA
CCCACGATTTCGGCAAGAACACCTATCACGGGCTCGAACTGTTGCGGGGATTGGACGTTCTTGTAAACAGCGGGTGGCCG
GTCCTGATGGCGCTGAGCAACAAGGACTTCATCGGGGAGACTCTAGGTGTCGGTCTTTCCGAACGACTGGAGGGCACATT
GGCAGCAACGGCGTGGTCCGCCGCCGCGGGCGCCCGGGTCTTCCGCGTTCACGAGGTCGCGCACACGCGGCGGGTGGTGG
ACATGATCGCCGCGATCCAGGGCATCCGGCCCCCCGCACGAACCCTGCGAGGTCTGGTATGA

Upstream 100 bases:

>100_bases
CCGGACGGACCGACGGCTACGTGCCCGCCTACGACGACTACCCCGCCGAGGTGGCGGCCGGGCGCGCGCCGAAGAACTGA
TCGGGGCCCTCGGTACAGTC

Downstream 100 bases:

>100_bases
AAATCCAACACCGCGAGTCCGCCTGGGCGAGCACGAATACCTGGGACAACCCCGAATGGGCGGTCGAGGAGCTGATCGCC
GCCAAGAACGGGCGCACCGT

Product: putative dihydropteroate synthase

Products: diphosphate; dihydropteroate [C]

Alternate protein names: DHPS 2; Dihydropteroate pyrophosphorylase 2 [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MVMFSPPAAPLPTLCGKPVATDRALVMAIVNRTPDSFYDRGATFTDEAAMAAVDRAVAEGADLVDIGGVKAGPGSEVDAA
VDIGGVKAGPGSEVDAAEETRRVVPFVAAIRAAYPDLLISVDTWRSEVARAAVAEGADLINDTWAGADPELVRVAAEHGA
GIVCSHTGGAVPRTRPHRVRYADVVAEVTETVVAAAERAAAAGVRTDSILIDPTHDFGKNTYHGLELLRGLDVLVNSGWP
VLMALSNKDFIGETLGVGLSERLEGTLAATAWSAAAGARVFRVHEVAHTRRVVDMIAAIQGIRPPARTLRGLV

Sequences:

>Translated_313_residues
MVMFSPPAAPLPTLCGKPVATDRALVMAIVNRTPDSFYDRGATFTDEAAMAAVDRAVAEGADLVDIGGVKAGPGSEVDAA
VDIGGVKAGPGSEVDAAEETRRVVPFVAAIRAAYPDLLISVDTWRSEVARAAVAEGADLINDTWAGADPELVRVAAEHGA
GIVCSHTGGAVPRTRPHRVRYADVVAEVTETVVAAAERAAAAGVRTDSILIDPTHDFGKNTYHGLELLRGLDVLVNSGWP
VLMALSNKDFIGETLGVGLSERLEGTLAATAWSAAAGARVFRVHEVAHTRRVVDMIAAIQGIRPPARTLRGLV
>Mature_313_residues
MVMFSPPAAPLPTLCGKPVATDRALVMAIVNRTPDSFYDRGATFTDEAAMAAVDRAVAEGADLVDIGGVKAGPGSEVDAA
VDIGGVKAGPGSEVDAAEETRRVVPFVAAIRAAYPDLLISVDTWRSEVARAAVAEGADLINDTWAGADPELVRVAAEHGA
GIVCSHTGGAVPRTRPHRVRYADVVAEVTETVVAAAERAAAAGVRTDSILIDPTHDFGKNTYHGLELLRGLDVLVNSGWP
VLMALSNKDFIGETLGVGLSERLEGTLAATAWSAAAGARVFRVHEVAHTRRVVDMIAAIQGIRPPARTLRGLV

Specific function: Has very low affinity for the DHPS substrate 6- hydroxymethyl-7,8-dihydropterin-pyrophosphate, but can bind the inhibitor dapsone. Seems to lack dihydropteroate synthase activity, and does probably not function in folate metabolism [H]

COG id: COG0294

COG function: function code H; Dihydropteroate synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082224, Length=272, Percent_Identity=36.0294117647059, Blast_Score=151, Evalue=5e-38,
Organism=Saccharomyces cerevisiae, GI6324073, Length=326, Percent_Identity=26.9938650306748, Blast_Score=95, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006390
- InterPro:   IPR011005
- InterPro:   IPR000489 [H]

Pfam domain/function: PF00809 Pterin_bind [H]

EC number: 2.5.1.15 [C]

Molecular weight: Translated: 32608; Mature: 32608

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS50972 PTERIN_BINDING ; PS00792 DHPS_1 ; PS00793 DHPS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVMFSPPAAPLPTLCGKPVATDRALVMAIVNRTPDSFYDRGATFTDEAAMAAVDRAVAEG
CEEECCCCCCCHHHCCCCCCCCHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHCC
ADLVDIGGVKAGPGSEVDAAVDIGGVKAGPGSEVDAAEETRRVVPFVAAIRAAYPDLLIS
CCEEEECCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHEEE
VDTWRSEVARAAVAEGADLINDTWAGADPELVRVAAEHGAGIVCSHTGGAVPRTRPHRVR
HHHHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHH
YADVVAEVTETVVAAAERAAAAGVRTDSILIDPTHDFGKNTYHGLELLRGLDVLVNSGWP
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHCCCHHHHHHHHHHHHHHHCCCCC
VLMALSNKDFIGETLGVGLSERLEGTLAATAWSAAAGARVFRVHEVAHTRRVVDMIAAIQ
EEEEECCCCHHHHHHCCCHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
GIRPPARTLRGLV
CCCCHHHHHCCCC
>Mature Secondary Structure
MVMFSPPAAPLPTLCGKPVATDRALVMAIVNRTPDSFYDRGATFTDEAAMAAVDRAVAEG
CEEECCCCCCCHHHCCCCCCCCHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHCC
ADLVDIGGVKAGPGSEVDAAVDIGGVKAGPGSEVDAAEETRRVVPFVAAIRAAYPDLLIS
CCEEEECCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHEEE
VDTWRSEVARAAVAEGADLINDTWAGADPELVRVAAEHGAGIVCSHTGGAVPRTRPHRVR
HHHHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHH
YADVVAEVTETVVAAAERAAAAGVRTDSILIDPTHDFGKNTYHGLELLRGLDVLVNSGWP
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHCCCHHHHHHHHHHHHHHHCCCCC
VLMALSNKDFIGETLGVGLSERLEGTLAATAWSAAAGARVFRVHEVAHTRRVVDMIAAIQ
EEEEECCCCHHHHHHCCCHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
GIRPPARTLRGLV
CCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0015 {4-aminobenzoate}} [C]

Substrates: @AMIN04*Amino-hydroxy-hydroxymethyldihydropteridinediphosphate!; aminobenzoate [C]

Specific reaction: @AMIN04.txt*Amino-hydroxy-hydroxymethyldihydropteridinediphosphate! + aminobenzoate = diphosphate + dihydropteroate [C]

General reaction: Aryl group transfer [C]

Inhibitor: Sulfaguanidine; Sulfanilamide; Sulfathiazole; Sulfonamides [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]