Click here to switch to the map view.

The map label for this gene is hpaB [H]

Identifier: 54026336

GI number: 54026336

Start: 4527382

End: 4529001

Strand: Reverse

Name: hpaB [H]

Synonym: nfa43650

Alternate gene names: 54026336

Gene position: 4529001-4527382 (Counterclockwise)

Preceding gene: 54026337

Following gene: 54026335

Centisome position: 75.22

GC content: 66.23

Gene sequence:

>1620_bases
ATGACCACCGTGGAAACCCGGGACGGCGGCCTGTCCGTCGATCCGAGCGTCGCGAATCCCGCCGCCGACCATCCGGCCAA
CAACCGGAAGAATTTCGCTTCTCGCCCGATGACCGGCGCGGAGTACATCGAGTCGTTGCGGGACGGGCGCGAGATCTGGC
TGCACGGCGAGCGCGTCGCCGACGTCACCACCCATCCGGCGTTCCGCAACCCGATCCGGATGACCGCGCGCCTGTACGAT
TCGCTGCATTCGGGCCCGCACACCGACGTGGTGACCGCGCCCACCGACACCGGCAACGGCGGCGTCACCATGCCGTTCTT
CAAGACCCCGACCAGCGCCGCCGACCTGGTGCGCGAACGCGACGCCATCGCCGCCTGGGCGCGGATGACCTACGGCTGGA
TGGGCCGCTCGCCCGACTACAAGGCCAGCTTCCTGGGCACGCTGCACGCCAACAAGGAGCTGTACGCGCCGTTCCAGGAC
AACGCCGAGCGGTGGTACCGGGAGTCGCAGGAGAAGGTCCTGTACTGGAACCACGCGATCATCAACCCGCCGGTGGACCG
GCAGCTGCCGCCCGACGAGGTGGGCGATGTGTTCATGAAGGTGGAGAAGGAGACCGACGCCGGGCTGATCGTGTCCGGGG
CGAAGGTGGTGGCCACCGGCTCGGCCATCACCAACTACAACTTCATCGCCCACTACGGCCTGCCGATCAAGAAGAAGGAA
TTCGCCCTGATCTGCACGGTGCCGATGGATGCGCCCGGGGTGAAACTGATCTGCCGCTCCTCCTACACCCAGCACGCGGC
GGTGATGGGCAGCCCGTTCGACTACCCGCTGTCGAGCCGGATGGACGAGAACGACACCATTTTCGTCTTCGACAAGGTGC
TGGTGCCGTGGGAGAACGTCTTCATGTACGGGGACGTGGACAAGATCAACGCCTTCTTCCCGCAGTCGGGGTTCCTGCCG
CGGTTCACCTTCCAGGGCTGCACCCGCTTGGCGGTCAAGCTCGACTTCATCGCCGGGCTGCTGCTCAAGGCCCTCGACGC
CACCGGCGCCGGCGGGTTCCGTGGCGTGCAGACCCGGGTCGGCGAGGTGATCGGCTGGCGCAACCTGTTCTGGACGCTCA
CCGACGCGATGGCCAACAACCCCGAACCGTGGATCGGTGACACCGTGATCCCGCGCCTCGAATACGGGCTCACCTACCGG
ATGTTCATGATCCAGGGCTATCCGCGGATCAAGGAGATCATCGAGCAGGACGTGGCCTCGGGCCTGATCTACCTGCCGTC
CTCGGCCGCCGATTTCAAGAGCCCCGAGGTGCGGCCGTACCTGGACAAGTACGTGCGCGGCTCGGACGGCATCACCGCCG
TGGACCGGGTCAAGGTCATGAAGGCCCTCTGGGATTCGATCGGCTCGGAATTCGGTGGGCGCCATGAACTCTACGAGCGC
AACTACTCGGGCAACCACGAGAACGTCAAGGCCGAACTGTTGTTCGCGGCGCAGAACCGTGGCGGGGTGAGCCGGATGCG
CGGGCTGGCCGAGCAGTGCCTGGCCGAGTACGACCTCGACGGCTGGACGGTGCCGGACCTGATCGGCAACGAGGACGTCA
GCTACTTCGGCGCCCGCTGA

Upstream 100 bases:

>100_bases
CTCGATGGACGACCCGCACACCGGCTGGTTCGGCGCCGACGCGGTGCTCGCGGCGCAACCGCTGGGCTGACAGCTTTCCC
CTCACACCGAAGGAGTTCTG

Downstream 100 bases:

>100_bases
CGGTGGTGCCCGGCGGTCCGGGTCGACAACGACGTCGTCGGTGGCAACGGCGCCACCCCGGACCGCCGAGCCGAAGAACA
TCGGAAACTAGGGTTGGTTC

Product: putative phenol hydroxylase large subunit

Products: NA

Alternate protein names: 4-HPA 3-hydroxylase; 4-HPA 3-monooxygenase large component [H]

Number of amino acids: Translated: 539; Mature: 538

Protein sequence:

>539_residues
MTTVETRDGGLSVDPSVANPAADHPANNRKNFASRPMTGAEYIESLRDGREIWLHGERVADVTTHPAFRNPIRMTARLYD
SLHSGPHTDVVTAPTDTGNGGVTMPFFKTPTSAADLVRERDAIAAWARMTYGWMGRSPDYKASFLGTLHANKELYAPFQD
NAERWYRESQEKVLYWNHAIINPPVDRQLPPDEVGDVFMKVEKETDAGLIVSGAKVVATGSAITNYNFIAHYGLPIKKKE
FALICTVPMDAPGVKLICRSSYTQHAAVMGSPFDYPLSSRMDENDTIFVFDKVLVPWENVFMYGDVDKINAFFPQSGFLP
RFTFQGCTRLAVKLDFIAGLLLKALDATGAGGFRGVQTRVGEVIGWRNLFWTLTDAMANNPEPWIGDTVIPRLEYGLTYR
MFMIQGYPRIKEIIEQDVASGLIYLPSSAADFKSPEVRPYLDKYVRGSDGITAVDRVKVMKALWDSIGSEFGGRHELYER
NYSGNHENVKAELLFAAQNRGGVSRMRGLAEQCLAEYDLDGWTVPDLIGNEDVSYFGAR

Sequences:

>Translated_539_residues
MTTVETRDGGLSVDPSVANPAADHPANNRKNFASRPMTGAEYIESLRDGREIWLHGERVADVTTHPAFRNPIRMTARLYD
SLHSGPHTDVVTAPTDTGNGGVTMPFFKTPTSAADLVRERDAIAAWARMTYGWMGRSPDYKASFLGTLHANKELYAPFQD
NAERWYRESQEKVLYWNHAIINPPVDRQLPPDEVGDVFMKVEKETDAGLIVSGAKVVATGSAITNYNFIAHYGLPIKKKE
FALICTVPMDAPGVKLICRSSYTQHAAVMGSPFDYPLSSRMDENDTIFVFDKVLVPWENVFMYGDVDKINAFFPQSGFLP
RFTFQGCTRLAVKLDFIAGLLLKALDATGAGGFRGVQTRVGEVIGWRNLFWTLTDAMANNPEPWIGDTVIPRLEYGLTYR
MFMIQGYPRIKEIIEQDVASGLIYLPSSAADFKSPEVRPYLDKYVRGSDGITAVDRVKVMKALWDSIGSEFGGRHELYER
NYSGNHENVKAELLFAAQNRGGVSRMRGLAEQCLAEYDLDGWTVPDLIGNEDVSYFGAR
>Mature_538_residues
TTVETRDGGLSVDPSVANPAADHPANNRKNFASRPMTGAEYIESLRDGREIWLHGERVADVTTHPAFRNPIRMTARLYDS
LHSGPHTDVVTAPTDTGNGGVTMPFFKTPTSAADLVRERDAIAAWARMTYGWMGRSPDYKASFLGTLHANKELYAPFQDN
AERWYRESQEKVLYWNHAIINPPVDRQLPPDEVGDVFMKVEKETDAGLIVSGAKVVATGSAITNYNFIAHYGLPIKKKEF
ALICTVPMDAPGVKLICRSSYTQHAAVMGSPFDYPLSSRMDENDTIFVFDKVLVPWENVFMYGDVDKINAFFPQSGFLPR
FTFQGCTRLAVKLDFIAGLLLKALDATGAGGFRGVQTRVGEVIGWRNLFWTLTDAMANNPEPWIGDTVIPRLEYGLTYRM
FMIQGYPRIKEIIEQDVASGLIYLPSSAADFKSPEVRPYLDKYVRGSDGITAVDRVKVMKALWDSIGSEFGGRHELYERN
YSGNHENVKAELLFAAQNRGGVSRMRGLAEQCLAEYDLDGWTVPDLIGNEDVSYFGAR

Specific function: Utilizes FADH(2) supplied by hpaC or by another flavin reductase, to catalyze the hydroxylation of 4-hydroxyphenylacetic acid, leading to the production of 3,4-DHPA. Can also oxidize phenol to catechol, and hydroxylate other phenol derivatives [H]

COG id: COG2368

COG function: function code Q; Aromatic ring hydroxylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FADH(2)-utilizing monooxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR009100
- InterPro:   IPR004925
- InterPro:   IPR012688 [H]

Pfam domain/function: PF03241 HpaB [H]

EC number: NA

Molecular weight: Translated: 60108; Mature: 59976

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTVETRDGGLSVDPSVANPAADHPANNRKNFASRPMTGAEYIESLRDGREIWLHGERVA
CCEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHCCCEEEEECCEEE
DVTTHPAFRNPIRMTARLYDSLHSGPHTDVVTAPTDTGNGGVTMPFFKTPTSAADLVRER
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCEECCEECCCCHHHHHHHHH
DAIAAWARMTYGWMGRSPDYKASFLGTLHANKELYAPFQDNAERWYRESQEKVLYWNHAI
HHHHHHHHHHHHCCCCCCCCCHHEEEEEECCCEEECCCCCCHHHHHHCCCCEEEEEECEE
INPPVDRQLPPDEVGDVFMKVEKETDAGLIVSGAKVVATGSAITNYNFIAHYGLPIKKKE
ECCCCCCCCCHHHHHHHHEEEECCCCCCEEEECCEEEEECCCCCCCCEEEECCCCCCCCC
FALICTVPMDAPGVKLICRSSYTQHAAVMGSPFDYPLSSRMDENDTIFVFDKVLVPWENV
EEEEEECCCCCCCCEEEECCCCCHHHHHCCCCCCCCHHCCCCCCCEEEEEEEEECCCCCE
FMYGDVDKINAFFPQSGFLPRFTFQGCTRLAVKLDFIAGLLLKALDATGAGGFRGVQTRV
EEECCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
GEVIGWRNLFWTLTDAMANNPEPWIGDTVIPRLEYGLTYRMFMIQGYPRIKEIIEQDVAS
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHC
GLIYLPSSAADFKSPEVRPYLDKYVRGSDGITAVDRVKVMKALWDSIGSEFGGRHELYER
CEEECCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHC
NYSGNHENVKAELLFAAQNRGGVSRMRGLAEQCLAEYDLDGWTVPDLIGNEDVSYFGAR
CCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCHHCCCC
>Mature Secondary Structure 
TTVETRDGGLSVDPSVANPAADHPANNRKNFASRPMTGAEYIESLRDGREIWLHGERVA
CEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHCCCEEEEECCEEE
DVTTHPAFRNPIRMTARLYDSLHSGPHTDVVTAPTDTGNGGVTMPFFKTPTSAADLVRER
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCEECCEECCCCHHHHHHHHH
DAIAAWARMTYGWMGRSPDYKASFLGTLHANKELYAPFQDNAERWYRESQEKVLYWNHAI
HHHHHHHHHHHHCCCCCCCCCHHEEEEEECCCEEECCCCCCHHHHHHCCCCEEEEEECEE
INPPVDRQLPPDEVGDVFMKVEKETDAGLIVSGAKVVATGSAITNYNFIAHYGLPIKKKE
ECCCCCCCCCHHHHHHHHEEEECCCCCCEEEECCEEEEECCCCCCCCEEEECCCCCCCCC
FALICTVPMDAPGVKLICRSSYTQHAAVMGSPFDYPLSSRMDENDTIFVFDKVLVPWENV
EEEEEECCCCCCCCEEEECCCCCHHHHHCCCCCCCCHHCCCCCCCEEEEEEEEECCCCCE
FMYGDVDKINAFFPQSGFLPRFTFQGCTRLAVKLDFIAGLLLKALDATGAGGFRGVQTRV
EEECCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
GEVIGWRNLFWTLTDAMANNPEPWIGDTVIPRLEYGLTYRMFMIQGYPRIKEIIEQDVAS
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHC
GLIYLPSSAADFKSPEVRPYLDKYVRGSDGITAVDRVKVMKALWDSIGSEFGGRHELYER
CEEECCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHC
NYSGNHENVKAELLFAAQNRGGVSRMRGLAEQCLAEYDLDGWTVPDLIGNEDVSYFGAR
CCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8077235; 8550403; 10653707 [H]