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The map label for this gene is yfiK [H]

Identifier: 54026217

GI number: 54026217

Start: 4396845

End: 4397537

Strand: Reverse

Name: yfiK [H]

Synonym: nfa42460

Alternate gene names: 54026217

Gene position: 4397537-4396845 (Counterclockwise)

Preceding gene: 54026218

Following gene: 54026216

Centisome position: 73.03

GC content: 71.57

Gene sequence:

>693_bases
GTGACGGCCGCGCACGGGGCCGACGACCGCCCGCTGACCGTCCTGGTCGTCGACGATCAGGAACTGGTGCGCGGCGGACT
ACGCCGCATCCTGCGCCGCCGCGACGGCTTCGTCGTGCACGAATGCGCCGACGGGGACGAGGTGTTCGCCGCCGTCGCCA
CCACCGATCCCGATGTGGTGCTGATGGATCTGCGGATGAAACGCCTCGGCGGCATCGACGCCACCCGTCAGCTGCGCTCG
CGGCCCGGTGCGCCGCCCGTGCTCGTGCTCACCACGTTCGACGACGACCAGCTGCTCTCGGGCGCGCTGCGGGCGGGCGC
CGCAGGCTTCCTGCTCAAGGACTCACCCGCCGAGGACCTGATCCGCGCCGTGCGGACCGTGGCCGGTGGTGGCGCCTGGC
TCGACCCGGCGGTCACCGGCCGGGTGCTCTCGGCCTACCGCACCGTGCGGCCGGCGCCCGCCCGGCGCGACGACCGGCTC
GCCGAGCTGACCGCCCGCGAGTACGAGGTGCTGGAGCTGATCGGCCGGGGCCGGGTGAACGCGGAGATCGCCACCGAACT
CGGGATCTCCGAGGTCACCGTGAAAAGTCATGTGGGCCATATCTTCGGCAAGCTCGACCTGCGCGATCGCGCCGCCGCCA
TCGTCTTCGCGTTTGACCACGGGGTGGTCTCACCAGGAGAATCCAACATCTGA

Upstream 100 bases:

>100_bases
CCGCGGGTCCGGTCGACGACGGGTGGAGCGTGCGCGCCCGGTTCCCGCTGGCCGGTGGGCGCGCCTGGCCGCTGTGCTCG
GGCGAGAGCGGGGAGCGCGG

Downstream 100 bases:

>100_bases
CGGGCGGCTGGAAGCCCACGCCGCGGCCCGAGACTTGGAGGTTGCACGGCGGTGGACGGACCTGGACGGCGAGTCGGTAC
CCGATTCGGCCCGTACGAGC

Product: putative two-component system response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MTAAHGADDRPLTVLVVDDQELVRGGLRRILRRRDGFVVHECADGDEVFAAVATTDPDVVLMDLRMKRLGGIDATRQLRS
RPGAPPVLVLTTFDDDQLLSGALRAGAAGFLLKDSPAEDLIRAVRTVAGGGAWLDPAVTGRVLSAYRTVRPAPARRDDRL
AELTAREYEVLELIGRGRVNAEIATELGISEVTVKSHVGHIFGKLDLRDRAAAIVFAFDHGVVSPGESNI

Sequences:

>Translated_230_residues
MTAAHGADDRPLTVLVVDDQELVRGGLRRILRRRDGFVVHECADGDEVFAAVATTDPDVVLMDLRMKRLGGIDATRQLRS
RPGAPPVLVLTTFDDDQLLSGALRAGAAGFLLKDSPAEDLIRAVRTVAGGGAWLDPAVTGRVLSAYRTVRPAPARRDDRL
AELTAREYEVLELIGRGRVNAEIATELGISEVTVKSHVGHIFGKLDLRDRAAAIVFAFDHGVVSPGESNI
>Mature_229_residues
TAAHGADDRPLTVLVVDDQELVRGGLRRILRRRDGFVVHECADGDEVFAAVATTDPDVVLMDLRMKRLGGIDATRQLRSR
PGAPPVLVLTTFDDDQLLSGALRAGAAGFLLKDSPAEDLIRAVRTVAGGGAWLDPAVTGRVLSAYRTVRPAPARRDDRLA
ELTAREYEVLELIGRGRVNAEIATELGISEVTVKSHVGHIFGKLDLRDRAAAIVFAFDHGVVSPGESNI

Specific function: Member of the two-component regulatory system yfiJ/yfiK [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=214, Percent_Identity=30.8411214953271, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1788521, Length=211, Percent_Identity=35.0710900473934, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1790102, Length=199, Percent_Identity=33.6683417085427, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1787473, Length=211, Percent_Identity=33.6492890995261, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1788712, Length=206, Percent_Identity=23.3009708737864, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1786747, Length=209, Percent_Identity=23.444976076555, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1788550, Length=100, Percent_Identity=35, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 24749; Mature: 24617

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAAHGADDRPLTVLVVDDQELVRGGLRRILRRRDGFVVHECADGDEVFAAVATTDPDVV
CCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEECCCCCEE
LMDLRMKRLGGIDATRQLRSRPGAPPVLVLTTFDDDQLLSGALRAGAAGFLLKDSPAEDL
EEEHHHHHHCCCCHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHCCCCCEEECCCCHHHH
IRAVRTVAGGGAWLDPAVTGRVLSAYRTVRPAPARRDDRLAELTAREYEVLELIGRGRVN
HHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
AEIATELGISEVTVKSHVGHIFGKLDLRDRAAAIVFAFDHGVVSPGESNI
HHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
TAAHGADDRPLTVLVVDDQELVRGGLRRILRRRDGFVVHECADGDEVFAAVATTDPDVV
CCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEECCCCCEE
LMDLRMKRLGGIDATRQLRSRPGAPPVLVLTTFDDDQLLSGALRAGAAGFLLKDSPAEDL
EEEHHHHHHCCCCHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHCCCCCEEECCCCHHHH
IRAVRTVAGGGAWLDPAVTGRVLSAYRTVRPAPARRDDRLAELTAREYEVLELIGRGRVN
HHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
AEIATELGISEVTVKSHVGHIFGKLDLRDRAAAIVFAFDHGVVSPGESNI
HHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8973323; 9384377 [H]