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The map label for this gene is ilvB [H]

Identifier: 54026203

GI number: 54026203

Start: 4378985

End: 4380928

Strand: Reverse

Name: ilvB [H]

Synonym: nfa42320

Alternate gene names: 54026203

Gene position: 4380928-4378985 (Counterclockwise)

Preceding gene: 54026206

Following gene: 54026202

Centisome position: 72.76

GC content: 70.22

Gene sequence:

>1944_bases
GTGAGCGCACCAACCGCACGACCCGGGCCCTCGGCCCGCAGGTCAGCGCCTTCGACGAATCAGCAGGCCACGCCTGCTGC
CGCTACCACGGCGAACCGCCGCCAGGTTCCGCCCGAGCGAGTGACCGGCGCCCAGTCGGTCGTGCGCTCGCTCGAGGAGC
TCGGCGTCGACACCGTCTTCGGCATTCCCGGCGGCGCGATCCTGCCCGTCTACGACCCGCTCTTCGACTCCACCAAGGTG
CGTCACGTGCTGGTCCGCCACGAGCAGGGCGCAGGCCACGCCGCGACCGGCTACGCGCAGGCCACCGGCAAGGTCGGTGT
GTGCATGGCCACATCAGGGCCCGGCGCGACCAACCTGGTGACCCCGATCGCCGACGCCCAGATGGACTCGGTGCCGATCG
TCGCCATCACCGGGCAGGTCGGCCGCGGCCTGATCGGCACCGACGCCTTCCAGGAAGCCGACATCTCCGGCATCACCATG
CCGATCACCAAGCACAACTTCCTGATCACCGACGGCATCGACATCCCGCGCATCATCGCCGAGGCGTTCTACCTGGCCGC
CTCCGGCCGCCCCGGCGCGGTGCTGGTCGACATCCCCAAGGACGTGCTGCAGGCGCAGACCACCTTCAGCTGGCCGCCGG
AGATGCGGCTGCCCGGCTACCGTCCGGTCACCAAGCCGCACGGCAAGCAGGTGCGCGAGGCCGCGCGGATGATCCTCGAG
TCCAAGGCCCCCGTGCTCTACGTGGGCGGCGGCGTCATCAAGGCCGACGCCTCGGCCGAGCTGCTGGAGCTGGCCGAACT
CACCGGCATCCCGGTGGTCACCACCCTGATGGCGCGCGGCGCGTTCCCCGACAGCCACCAGCTCAACATGGGCATGCCCG
GCATGCACGGCACCGTCGGCGCGGTCGCCGCGCTGCAGAAGGCCGACCTGCTCATCACCCTCGGCGCGCGGTTCGACGAC
CGCGTCACCGGGCAGCTGGACTCCTTCGCACCCGGCGCCAAGGTCATCCACGCCGACATCGACCCGGCCGAGATCGGCAA
GAACCGGCACGCCGACGTGCCGATCGTGGGCGACTGCCGCGAGGTGATCGCCGAGCTCATCGAGACGCTCAAGGCCGAGC
CGACCGCGGGCGAGGCCCCGCTGCTGAACCTCACCGAGTGGTGGACCTACCTCGACGGCGTGCGCAAGCAGTACCCGCTG
GGCTGGACCCCGCCCAGCGACGGCTCGCTCTCGCCCGAGTTCGTCATCGAGGCGCTCGGCCGCCTGGCCGGCCCCGATGC
CATCTACTGCGCGGGCGTCGGCCAGCACCAGATGTGGGCGGCCCAGTTCATCAAGTACGAGAAGCCGCGCACCTGGCTCA
ACTCCGGCGGCCTCGGCACCATGGGCTACGCCGTGCCCGCCGCGATGGGCGCCAAGATGGGCATGCCCGACCGCGAGGTC
TGGGCGGTCGACGGCGACGGCTGCTTCCAGATGACCAACCAGGAGCTGGCCACCTGCGCGGTGGAAGGCGTGCCGATCAA
GGTCGCGCTGATCAACAACGGCAACCTCGGCATGGTCCGCCAGTGGCAGACCCTGTTCTACGAGGAGCGCTACTCCAACA
CCGACCTGGGCACCCACACCCTGCGCATCCCCGATTTCGTCAAGCTCGCCGAAGCGCTCGGCTGCCACGGCATCCGGGTC
GAGAAGGAAGAGGACGTCGAGGCCGCGATCCGCGAGGCGCAGTCCATCAACGACCGCCCCGTCGTCATCGACTTCATCGT
CGGCAAGGACGCGCAGGTGTGGCCGATGGTCGCCGCGGGCACCAGCAACGACGAGATCATGGCCGCGCGCGGCATCCGGC
CGCTCTTCGACGAGGACGAGCAGGCCGCCGAGCCCGCCGTCATCCACGAGGCGATGACCCACGGAGATCGCGCGGCCGAG
CAGAACAAGGGGACCGAAGAATGA

Upstream 100 bases:

>100_bases
TGTTCGGACATCGTCCGGCACGGCGGGTCAGCGGACGCCGGTCGTGCCAGGCACACAAGTGTTGTCCGGGTTCGGAACGA
CAAGTGAGGAAACGAAGACG

Downstream 100 bases:

>100_bases
GCAGCACCCACACCCTGAGCGTCCTGGTCGAGGACAAGCCGGGCGTGCTGGCGCGGGTGGCGAGCCTGTTCTCCCGCCGC
GGGTTCAACATCGAATCGCT

Product: acetolactate synthase 1 catalytic subunit

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase large subunit; AHAS [H]

Number of amino acids: Translated: 647; Mature: 646

Protein sequence:

>647_residues
MSAPTARPGPSARRSAPSTNQQATPAAATTANRRQVPPERVTGAQSVVRSLEELGVDTVFGIPGGAILPVYDPLFDSTKV
RHVLVRHEQGAGHAATGYAQATGKVGVCMATSGPGATNLVTPIADAQMDSVPIVAITGQVGRGLIGTDAFQEADISGITM
PITKHNFLITDGIDIPRIIAEAFYLAASGRPGAVLVDIPKDVLQAQTTFSWPPEMRLPGYRPVTKPHGKQVREAARMILE
SKAPVLYVGGGVIKADASAELLELAELTGIPVVTTLMARGAFPDSHQLNMGMPGMHGTVGAVAALQKADLLITLGARFDD
RVTGQLDSFAPGAKVIHADIDPAEIGKNRHADVPIVGDCREVIAELIETLKAEPTAGEAPLLNLTEWWTYLDGVRKQYPL
GWTPPSDGSLSPEFVIEALGRLAGPDAIYCAGVGQHQMWAAQFIKYEKPRTWLNSGGLGTMGYAVPAAMGAKMGMPDREV
WAVDGDGCFQMTNQELATCAVEGVPIKVALINNGNLGMVRQWQTLFYEERYSNTDLGTHTLRIPDFVKLAEALGCHGIRV
EKEEDVEAAIREAQSINDRPVVIDFIVGKDAQVWPMVAAGTSNDEIMAARGIRPLFDEDEQAAEPAVIHEAMTHGDRAAE
QNKGTEE

Sequences:

>Translated_647_residues
MSAPTARPGPSARRSAPSTNQQATPAAATTANRRQVPPERVTGAQSVVRSLEELGVDTVFGIPGGAILPVYDPLFDSTKV
RHVLVRHEQGAGHAATGYAQATGKVGVCMATSGPGATNLVTPIADAQMDSVPIVAITGQVGRGLIGTDAFQEADISGITM
PITKHNFLITDGIDIPRIIAEAFYLAASGRPGAVLVDIPKDVLQAQTTFSWPPEMRLPGYRPVTKPHGKQVREAARMILE
SKAPVLYVGGGVIKADASAELLELAELTGIPVVTTLMARGAFPDSHQLNMGMPGMHGTVGAVAALQKADLLITLGARFDD
RVTGQLDSFAPGAKVIHADIDPAEIGKNRHADVPIVGDCREVIAELIETLKAEPTAGEAPLLNLTEWWTYLDGVRKQYPL
GWTPPSDGSLSPEFVIEALGRLAGPDAIYCAGVGQHQMWAAQFIKYEKPRTWLNSGGLGTMGYAVPAAMGAKMGMPDREV
WAVDGDGCFQMTNQELATCAVEGVPIKVALINNGNLGMVRQWQTLFYEERYSNTDLGTHTLRIPDFVKLAEALGCHGIRV
EKEEDVEAAIREAQSINDRPVVIDFIVGKDAQVWPMVAAGTSNDEIMAARGIRPLFDEDEQAAEPAVIHEAMTHGDRAAE
QNKGTEE
>Mature_646_residues
SAPTARPGPSARRSAPSTNQQATPAAATTANRRQVPPERVTGAQSVVRSLEELGVDTVFGIPGGAILPVYDPLFDSTKVR
HVLVRHEQGAGHAATGYAQATGKVGVCMATSGPGATNLVTPIADAQMDSVPIVAITGQVGRGLIGTDAFQEADISGITMP
ITKHNFLITDGIDIPRIIAEAFYLAASGRPGAVLVDIPKDVLQAQTTFSWPPEMRLPGYRPVTKPHGKQVREAARMILES
KAPVLYVGGGVIKADASAELLELAELTGIPVVTTLMARGAFPDSHQLNMGMPGMHGTVGAVAALQKADLLITLGARFDDR
VTGQLDSFAPGAKVIHADIDPAEIGKNRHADVPIVGDCREVIAELIETLKAEPTAGEAPLLNLTEWWTYLDGVRKQYPLG
WTPPSDGSLSPEFVIEALGRLAGPDAIYCAGVGQHQMWAAQFIKYEKPRTWLNSGGLGTMGYAVPAAMGAKMGMPDREVW
AVDGDGCFQMTNQELATCAVEGVPIKVALINNGNLGMVRQWQTLFYEERYSNTDLGTHTLRIPDFVKLAEALGCHGIRVE
KEEDVEAAIREAQSINDRPVVIDFIVGKDAQVWPMVAAGTSNDEIMAARGIRPLFDEDEQAAEPAVIHEAMTHGDRAAEQ
NKGTEE

Specific function: Catalyzes the conversion of 2 pyruvate molecules into acetolactate in the first common step of the biosynthetic pathway of the branched-amino acids such as leucine, isoleucine, and valine. Also involved in condensing pyruvate and 2-ketobutyrate to form 2-

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=569, Percent_Identity=26.1862917398945, Blast_Score=172, Evalue=6e-43,
Organism=Homo sapiens, GI21361361, Length=597, Percent_Identity=27.1356783919598, Blast_Score=151, Evalue=2e-36,
Organism=Escherichia coli, GI87081685, Length=579, Percent_Identity=45.4231433506045, Blast_Score=514, Evalue=1e-147,
Organism=Escherichia coli, GI1790104, Length=584, Percent_Identity=44.6917808219178, Blast_Score=466, Evalue=1e-132,
Organism=Escherichia coli, GI1786717, Length=569, Percent_Identity=36.7311072056239, Blast_Score=356, Evalue=2e-99,
Organism=Escherichia coli, GI1787096, Length=563, Percent_Identity=29.3072824156306, Blast_Score=214, Evalue=2e-56,
Organism=Escherichia coli, GI1788716, Length=487, Percent_Identity=27.5154004106776, Blast_Score=154, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17531299, Length=597, Percent_Identity=26.2981574539363, Blast_Score=147, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17531301, Length=597, Percent_Identity=26.2981574539363, Blast_Score=147, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17542570, Length=519, Percent_Identity=26.3969171483622, Blast_Score=134, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6323755, Length=630, Percent_Identity=42.2222222222222, Blast_Score=455, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6320816, Length=499, Percent_Identity=24.6492985971944, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6323163, Length=499, Percent_Identity=22.8456913827655, Blast_Score=85, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6323073, Length=502, Percent_Identity=23.7051792828685, Blast_Score=84, Evalue=5e-17,
Organism=Drosophila melanogaster, GI19922626, Length=581, Percent_Identity=26.6781411359725, Blast_Score=182, Evalue=7e-46,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 68983; Mature: 68852

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPTARPGPSARRSAPSTNQQATPAAATTANRRQVPPERVTGAQSVVRSLEELGVDTVF
CCCCCCCCCCCHHCCCCCCCCCCCCCHHCCCCCCCCCHHHHCCHHHHHHHHHHCCCCEEE
GIPGGAILPVYDPLFDSTKVRHVLVRHEQGAGHAATGYAQATGKVGVCMATSGPGATNLV
CCCCCCEEECCCCCCCCHHHEEEEEEECCCCCCCCCCHHCCCCCEEEEEECCCCCCCCCC
TPIADAQMDSVPIVAITGQVGRGLIGTDAFQEADISGITMPITKHNFLITDGIDIPRIIA
CCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHCCCCCEEEEEECCCEEEECCCCHHHHHH
EAFYLAASGRPGAVLVDIPKDVLQAQTTFSWPPEMRLPGYRPVTKPHGKQVREAARMILE
HHHHHEECCCCCEEEEECCHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
SKAPVLYVGGGVIKADASAELLELAELTGIPVVTTLMARGAFPDSHQLNMGMPGMHGTVG
CCCCEEEECCEEEECCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHH
AVAALQKADLLITLGARFDDRVTGQLDSFAPGAKVIHADIDPAEIGKNRHADVPIVGDCR
HHHHHHHCCEEEEECCCCCCCCCCCHHCCCCCCEEEEECCCHHHHCCCCCCCCCEECCHH
EVIAELIETLKAEPTAGEAPLLNLTEWWTYLDGVRKQYPLGWTPPSDGSLSPEFVIEALG
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
RLAGPDAIYCAGVGQHQMWAAQFIKYEKPRTWLNSGGLGTMGYAVPAAMGAKMGMPDREV
HHCCCCEEEEECCCCHHHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHCCCCCCCCEE
WAVDGDGCFQMTNQELATCAVEGVPIKVALINNGNLGMVRQWQTLFYEERYSNTDLGTHT
EEECCCCCEEECCHHHHHHEECCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCEE
LRIPDFVKLAEALGCHGIRVEKEEDVEAAIREAQSINDRPVVIDFIVGKDAQVWPMVAAG
EECCHHHHHHHHHCCCCEEECCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEEEEC
TSNDEIMAARGIRPLFDEDEQAAEPAVIHEAMTHGDRAAEQNKGTEE
CCCCCEEEECCCCCCCCCCHHHCCHHHHHHHHHCCHHHHHCCCCCCC
>Mature Secondary Structure 
SAPTARPGPSARRSAPSTNQQATPAAATTANRRQVPPERVTGAQSVVRSLEELGVDTVF
CCCCCCCCCCHHCCCCCCCCCCCCCHHCCCCCCCCCHHHHCCHHHHHHHHHHCCCCEEE
GIPGGAILPVYDPLFDSTKVRHVLVRHEQGAGHAATGYAQATGKVGVCMATSGPGATNLV
CCCCCCEEECCCCCCCCHHHEEEEEEECCCCCCCCCCHHCCCCCEEEEEECCCCCCCCCC
TPIADAQMDSVPIVAITGQVGRGLIGTDAFQEADISGITMPITKHNFLITDGIDIPRIIA
CCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHCCCCCEEEEEECCCEEEECCCCHHHHHH
EAFYLAASGRPGAVLVDIPKDVLQAQTTFSWPPEMRLPGYRPVTKPHGKQVREAARMILE
HHHHHEECCCCCEEEEECCHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
SKAPVLYVGGGVIKADASAELLELAELTGIPVVTTLMARGAFPDSHQLNMGMPGMHGTVG
CCCCEEEECCEEEECCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHH
AVAALQKADLLITLGARFDDRVTGQLDSFAPGAKVIHADIDPAEIGKNRHADVPIVGDCR
HHHHHHHCCEEEEECCCCCCCCCCCHHCCCCCCEEEEECCCHHHHCCCCCCCCCEECCHH
EVIAELIETLKAEPTAGEAPLLNLTEWWTYLDGVRKQYPLGWTPPSDGSLSPEFVIEALG
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
RLAGPDAIYCAGVGQHQMWAAQFIKYEKPRTWLNSGGLGTMGYAVPAAMGAKMGMPDREV
HHCCCCEEEEECCCCHHHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHCCCCCCCCEE
WAVDGDGCFQMTNQELATCAVEGVPIKVALINNGNLGMVRQWQTLFYEERYSNTDLGTHT
EEECCCCCEEECCHHHHHHEECCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCEE
LRIPDFVKLAEALGCHGIRVEKEEDVEAAIREAQSINDRPVVIDFIVGKDAQVWPMVAAG
EECCHHHHHHHHHCCCCEEECCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEEEEC
TSNDEIMAARGIRPLFDEDEQAAEPAVIHEAMTHGDRAAEQNKGTEE
CCCCCEEEECCCCCCCCCCHHHCCHHHHHHHHHCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]